Microbiology Lab Exam 3

61 Questions | Total Attempts: 254

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Microbiology Lab Quizzes & Trivia

For the micro lab exam 3


Questions and Answers
  • 1. 
    ___ ___ is an example of an enriched, differential media that contains trypticase soy agar with 5 or 10% ___ ___ added to the agar
  • 2. 
    This is the change of appearance of the agar surrounding individual colonies when bacteria are able to utilize RBCs
  • 3. 
    The COMPLETE destruction ofhemoglobin within the RBCs, the media may look yellow
  • 4. 
    The PARTIAL destruction of the hemoglobin within the RBC's, the media appears green/brown around the individual colonies
  • 5. 
    NO destruction of the hemoglobin within the RBC's, the media appears red around the individual colonies
  • 6. 
    True or False: both Staphylococcus and Streptococcus species have the ability to break down hemoglobin, thus other methods ofdifferentiation of these species are required.
  • 7. 
    E.Focalis is an example of
  • 8. 
    In a bile esculin test, a black color represents a ___ reaction while a yellow color represents a ___ reaction
  • 9. 
    In a 6.5% NaCL test (salt) purple indicates ___ while yellow indicates ___
  • 10. 
    In a coagulase test, a clot indicates___ while a liquid indicates ___
  • 11. 
    In Mannitol Salt agar, yellow indicates a ___ use of mannitol while pink indicates a ___ use of mannitol
  • 12. 
    Mannitol Salt Agar inhibits gram ___
  • 13. 
    Mannitol Salt Agar is ___/___ that inhibits the growth of gram ___
  • 14. 
    The high salt content in Mannitol Salt Agar selects for ___
  • 15. 
    Biochemical testing can only be done on ___ cultures
  • 16. 
    This breaks down hydrogen peroxide into water and oxygen
  • 17. 
    ___ produce catalase, but ___ do not
  • 18. 
    ___ is a common component of our skin flora
  • 19. 
    ___ is an enzyme that causes fibrin in plasma to clot
  • 20. 
    ___ ___ is the only staphylococcus species that produces coagulase
  • 21. 
    A coagulase test is used to differentiate ___ from ___
  • 22. 
    The sugar ___ gives mannitol salt agar its differential properties
  • 23. 
    When mannitol is fermented (utilized by bacteria) microorganisms release acidic byproducts that change the PH of the surrounding media.  The PH change is indicated by a change in the PH indicator ___ ___ from pink to yellow
  • 24. 
    ___ is used to identify Group A strep, the cause of strept throat.  Group A strep are the only species group that aresensitive to this antibiotic and can be detected by a zone of inhibition around the __ __ __
  • 25. 
    ___ ___ testing is used to identify ___ which can cause urinary tract as well as opportunist infections
  • 26. 
    True or false: bile esculin testing incorporates two tests: the ability to grow in 40% bile and the ability to hydrolyze esculin that then combines with Fe+3 to form a black precipitate
  • 27. 
    ____ ____ is the only strept species that is sensitive to the drug ____ This can be detected by a zone of inhibition around a ___ ___ ___
  • 28. 
    One can determine whether a culture is positive for Streptococcus pyogenes because streptococcus has cocci and its sensitivity to ___ ___ ___
  • 29. 
    If you wanted to differentiate Enterococcus faecalis and Streptococcus bovis which test would you use?
  • 30. 
    Lysis of RBCs, total utilization of hemoglobin, yellow color of media around colony
  • 31. 
    Lysis of RBCs, partial utilization of hemoglobin, green brown color of media around colony
  • 32. 
    No lysis of RBCs, no utilization of hemoglobin, red around colonies
  • 33. 
    This sugar makes mannitol salt agar differential
  • 34. 
    This has a gamma hemolysis reaction, a positive bile esculin reaction, and a positive 6.5% NaCl reaction
  • 35. 
    This has a gamma hemolysis reaction, a positive bile esculin reaction, and a negative 6.5% NaCl reaction
  • 36. 
    This has a beta hemolysis reaction, and is sensitive to Taxo A bacitrin
  • 37. 
    This has a alpha hemolysis reaction, and is sensitive to Taxo P optochin
  • 38. 
    For a surface to be ___ there must be destruction of all life forms: vegetative cells,spores,and viruses.
  • 39. 
    Sterilization can be easily accomplished with the use of an ___
  • 40. 
    The destruction of pathogens on inanimate objects is the job of a
  • 41. 
    Disinfectants ___ achieve total killing of all life forms (always, never, rarely)
  • 42. 
    If a chemical is given the term ___ it should be able to inhibit or destroy microorganisms on living tissue in an attempt to prevent infection.
  • 43. 
    ___ are less antimicrobial than other chemicals
  • 44. 
    When preparing a culture, the process of rolling broth culture into three directions with overlapping strokes is called making a ___ ___ ___
  • 45. 
    True or False: Over incubating will often result in in overgrowth of the microorganism and show no zone of inhibition
  • 46. 
    Zone of inhibition is measured in
  • 47. 
    Rolling the bacteria out in 3 different directions until the plate is covered is called ___ the plate
  • 48. 
    This is a zone of no apparent growth
  • 49. 
    Does it mean that the bacteria are dead in a zone of inhibition? (Yes or No)
  • 50. 
    The disinfectants we used in lab were found to be more effective in gram ___ bacteria
  • 51. 
    The purpose of exercise 14 is to familiarize the student with the ___ ___ ___ ___ method for testing antimicrobial sensitivities
  • 52. 
    The ___ ___ ___ must be measured in millimeters and then compared with a ___ ___
  • 53. 
    Tetracycline is a gram ___ organism
  • 54. 
    Chloramphenicol is a gram ___ organism
  • 55. 
    In regards to table 14.1, the evaluation of zone of inhibition ___ is the zone size indicated in the chart and any measurement LESS THAN that number
  • 56. 
    In regards to table 14.1, the evaluation of zone of inhibition ___ is the zone size indicated in the chart and any measurement MORE THAN that number
  • 57. 
    Name two antibiotics that are the most effective against Staphylococcus epidermidis
  • 58. 
    Name two antibiotics that are the most effective against E Coli
  • 59. 
    Name an antibiotic would you not use for treating Staphylococcus epidermidis
  • 60. 
    Name an antibiotic you would not use for treating E Coli
  • 61. 
    Which of the antibiotics would be considered broad spectrum (positive and negative