Know About Electrophoresis- The Laboratory Quiz

17 Questions | Total Attempts: 65

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Know About Electrophoresis- The Laboratory Quiz

Electrophoresis is a laboratory technique used to separate DNA, RNA, or protein molecules based on the size and electrical charge. An electric current is used to move around molecules to be separated through a gel. Learn new facts about this interesting topic in this quiz about Electrophoresis.


Questions and Answers
  • 1. 
    Migration of small ions is known as
    • A. 

      Electropherogram

    • B. 

      Iontophoresis

    • C. 

      Zone electrophoresis

  • 2. 
    Charged molecules migrate as zones, usually in a porous supporting medium (agarose gel film) after the sample is mixed with a buffer solution is referred to as
    • A. 

      Iontophoresis

    • B. 

      Zone electrophoresis

    • C. 

      Electropherogram

  • 3. 
    The isoelectric point (pI) of an ampholytes molecule is
    • A. 

      The pH at which the molecule has no electrical charge & does not migrate within an electrical field

    • B. 

      The pH at which the molecule has electrical charge & does not migrate within an electrical field

  • 4. 
    A ampholyte molecule takes on a -ve charge in a solution that is more acidic than its pI & migrates toward the cathode
    • A. 

      True

    • B. 

      False

  • 5. 
    In a more alkaline solution, an ampholyte molecule is negatively ionized & migrates toward the anode
    • A. 

      True

    • B. 

      False

  • 6. 
    The speed of migration of ions in electric field depends upon:
    • A. 

      Shape and size of molecule

    • B. 

      Magnitude of charge and shape of molecule

    • C. 

      Magnitude of charge and mass of molecule

    • D. 

      Magnitude of charge shape and mass of molecule

  • 7. 
    Which of the following statements is true about Electrophoretic mobility or migration of biomolecules?
    • A. 

      The rate of migration is directly proportional to the resistance of medium

    • B. 

      Rate of migration is inversely proportional to current

    • C. 

      Rate of migration is directly proportional to net charge & inversely proportional to molecular size & viscosity

  • 8. 
    Other actors that affect Electrophoretic mobility include endosmotic flow and wick flow
    • A. 

      True

    • B. 

      False

  • 9. 
    Carries the applied current and Determines the electrical charge on the solute
    • A. 

      Buffer

    • B. 

      Support medium

  • 10. 
    The buffer’s ionic strength influences
    • A. 

      The conductance of the support

    • B. 

      The thickness of the ionic cloud (buffer & non buffer ions) surrounding a charged molecule

    • C. 

      The rate of its migration

    • D. 

      The sharpness of the electrophoretic zones

    • E. 

      All the above

  • 11. 
    Provides the matrix in which separation takes place
    • A. 

      Buffer

    • B. 

      Support media

  • 12. 
    1st material used as a support medium that Separate macromolecules (surface charge & molecular size)
    • A. 

      Starch gel

    • B. 

      Cellulose acetate

    • C. 

      Agarose

    • D. 

      Polyacrylamide

  • 13. 
    Which of the following support media is used  for the separation of a variety of analytes such as Serum, urine, or CSF proteins, Hemoglobin variants, Isoenzymes and Lipoproteins
    • A. 

      Starch gel  

    • B. 

      Cellulose acetate

    • C. 

      Agarose

    • D. 

      Polyacrylamide

  • 14. 
    What does the electrophoresis apparatus consist of?
    • A. 

       Gel, buffer chamber and fire pack

    • B. 

      Buffer chamber and electrophoresis unit

    • C. 

      Power pack and electrophoresis unit

  • 15. 
     what is agarose gel ?
    • A. 

      Cross linkage molecules

    • B. 

      Purified unchange poly saccharide

    • C. 

      It is prepared by dissolving 0.5% agarose in boiling water and allowing it to cool to 40°C

    • D. 

       All of the above

  • 16. 
    The average pore size in a typical 7.5% polyacrylamide gel is about 5 nm (50 Å)
    • A. 

      True

    • B. 

      False

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