Know About Electrophoresis- The Laboratory Quiz

17 Questions | By Themes | Updated: Mar 22, 2022 | Attempts: 468

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Know About Electrophoresis- The Laboratory Quiz - Quiz

Electrophoresis is a laboratory technique used to separate DNA, RNA, or protein molecules based on the size and electrical charge. An electric current is used to move around molecules to be separated through a gel. Learn new facts about this interesting topic in this quiz about Electrophoresis.

Questions and Answers

1.

Migration of small ions is known as
- A.
  
  Electropherogram
- B.
  
  Iontophoresis
- C.
  
  Zone electrophoresis
2.

Charged molecules migrate as zones, usually in a porous supporting medium (agarose gel film) after the sample is mixed with a buffer solution is referred to as
- A.
  
  Iontophoresis
- B.
  
  Zone electrophoresis
- C.
  
  Electropherogram
3.

The isoelectric point (pI) of an ampholytes molecule is
- A.
  
  The pH at which the molecule has no electrical charge & does not migrate within an electrical field
- B.
  
  The pH at which the molecule has electrical charge & does not migrate within an electrical field
4.

A ampholyte molecule takes on a -ve charge in a solution that is more acidic than its pI & migrates toward the cathode
- A.
  
  True
- B.
  
  False
5.

In a more alkaline solution, an ampholyte molecule is negatively ionized & migrates toward the anode
- A.
  
  True
- B.
  
  False
6.

The speed of migration of ions in electric field depends upon:
- A.
  
  Shape and size of molecule
- B.
  
  Magnitude of charge and shape of molecule
- C.
  
  Magnitude of charge and mass of molecule
- D.
  
  Magnitude of charge shape and mass of molecule
7.

Which of the following statements is true about Electrophoretic mobility or migration of biomolecules?
- A.
  
  The rate of migration is directly proportional to the resistance of medium
- B.
  
  Rate of migration is inversely proportional to current
- C.
  
  Rate of migration is directly proportional to net charge & inversely proportional to molecular size & viscosity
8.

Other actors that affect Electrophoretic mobility include endosmotic flow and wick flow
- A.
  
  True
- B.
  
  False
9.

Carries the applied current and Determines the electrical charge on the solute
- A.
  
  Buffer
- B.
  
  Support medium
10.

The buffer’s ionic strength influences
- A.
  
  The conductance of the support
- B.
  
  The thickness of the ionic cloud (buffer & non buffer ions) surrounding a charged molecule
- C.
  
  The rate of its migration
- D.
  
  The sharpness of the electrophoretic zones
- E.
  
  All the above
11.

Provides the matrix in which separation takes place
- A.
  
  Buffer
- B.
  
  Support media
12.

1st material used as a support medium that Separate macromolecules (surface charge & molecular size)
- A.
  
  Starch gel
- B.
  
  Cellulose acetate
- C.
  
  Agarose
- D.
  
  Polyacrylamide
13.

Which of the following support media is used for the separation of a variety of analytes such as Serum, urine, or CSF proteins, Hemoglobin variants, Isoenzymes and Lipoproteins
- A.
  
  Starch gel
- B.
  
  Cellulose acetate
- C.
  
  Agarose
- D.
  
  Polyacrylamide
14.

What does the electrophoresis apparatus consist of?
- A.
  
  Gel, buffer chamber and fire pack
- B.
  
  Buffer chamber and electrophoresis unit
- C.
  
  Power pack and electrophoresis unit
15.

what is agarose gel ?
- A.
  
  Cross linkage molecules
- B.
  
  Purified unchange poly saccharide
- C.
  
  It is prepared by dissolving 0.5% agarose in boiling water and allowing it to cool to 40°C
- D.
  
  All of the above
16.

The average pore size in a typical 7.5% polyacrylamide gel is about 5 nm (50 Å)
- A.
  
  True
- B.
  
  False