Know About Electrophoresis- The Laboratory Quiz
(269).jpg "Know About Electrophoresis- The Laboratory Quiz")
Electrophoresis is a laboratory technique used to separate DNA, RNA, or protein molecules based on the size and electrical charge. An electric current is used to move around molecules to be separated through a gel. Learn new facts about this interesting topic in this quiz about Electrophoresis.
- 1.Migration of small ions is known as
- A.
Electropherogram
- B.
Iontophoresis
- C.
Zone electrophoresis
- 2.Charged molecules migrate as zones, usually in a porous supporting medium (agarose gel film) after the sample is mixed with a buffer solution is referred to as
- A.
Iontophoresis
- B.
Zone electrophoresis
- C.
Electropherogram
- 3.The isoelectric point (pI) of an ampholytes molecule is
- A.
The pH at which the molecule has no electrical charge & does not migrate within an electrical field
- B.
The pH at which the molecule has electrical charge & does not migrate within an electrical field
- 4.A ampholyte molecule takes on a -ve charge in a solution that is more acidic than its pI & migrates toward the cathode
- A.
True
- B.
False
- 5.In a more alkaline solution, an ampholyte molecule is negatively ionized & migrates toward the anode
- A.
True
- B.
False
- 6.The speed of migration of ions in electric field depends upon:
- A.
Shape and size of molecule
- B.
Magnitude of charge and shape of molecule
- C.
Magnitude of charge and mass of molecule
- D.
Magnitude of charge shape and mass of molecule
- 7.Which of the following statements is true about Electrophoretic mobility or migration of biomolecules?
- A.
The rate of migration is directly proportional to the resistance of medium
- B.
Rate of migration is inversely proportional to current
- C.
Rate of migration is directly proportional to net charge & inversely proportional to molecular size & viscosity
- 8.Other actors that affect Electrophoretic mobility include endosmotic flow and wick flow
- A.
True
- B.
False
- 9.Carries the applied current and Determines the electrical charge on the solute
- A.
Buffer
- B.
Support medium
- 10.The buffer’s ionic strength influences
- A.
The conductance of the support
- B.
The thickness of the ionic cloud (buffer & non buffer ions) surrounding a charged molecule
- C.
The rate of its migration
- D.
The sharpness of the electrophoretic zones
- E.
All the above
- 11.Provides the matrix in which separation takes place
- A.
Buffer
- B.
Support media
- 12.1st material used as a support medium that Separate macromolecules (surface charge & molecular size)
- A.
Starch gel
- B.
Cellulose acetate
- C.
Agarose
- D.
Polyacrylamide
- 13.Which of the following support media is used for the separation of a variety of analytes such as Serum, urine, or CSF proteins, Hemoglobin variants, Isoenzymes and Lipoproteins
- A.
Starch gel
- B.
Cellulose acetate
- C.
Agarose
- D.
Polyacrylamide
- 14.What does the electrophoresis apparatus consist of?
- A.
Gel, buffer chamber and fire pack
- B.
Buffer chamber and electrophoresis unit
- C.
Power pack and electrophoresis unit
- 15.what is agarose gel ?
- A.
Cross linkage molecules
- B.
Purified unchange poly saccharide
- C.
It is prepared by dissolving 0.5% agarose in boiling water and allowing it to cool to 40°C
- D.
All of the above
- 16.The average pore size in a typical 7.5% polyacrylamide gel is about 5 nm (50 Å)
- A.
True
- B.
False
Questions: 9 | Attempts: 7549 | Last updated: Nov 12, 2020
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