Quiz: Real-Time PCR

10 Questions | By Xinhuey94vv | Last updated: May 14, 2021 | Total Attempts: 5259

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1.

Who received the Nobel Prize in Chemistry in 1993 for inventing PCR?
- A.
  
  David Baltimore
- B.
  
  Kary Mullis
- C.
  
  Robert Weinberg
- D.
  
  John Nash
2.

What does the acronym PCR stand for?
- A.
  
  Polymerase Chain Reaction
- B.
  
  Polymerase Copying Reaction
- C.
  
  Polymerase Copied Repeats
- D.
  
  Polymerase Chain Restoration
3.

This is the sequence order of RT-PCR: 1. Denature 2. Anneal 3. Detect 4. Extend
- A.
  
  True
- B.
  
  False
4.

Nucleotide triphosphates (dNTPs) are added to the growing DNA strand during the _______________ phase.
- A.
  
  Extension/elongation
- B.
  
  Annealing
- C.
  
  Denaturation/separation
- D.
  
  Preparation
5.

Which is/are the property of real-time PCR assays?

Discuss
- A.
  
  Incorporate dyes that bind double-stranded DNA.
- B.
  
  Incorporate an internal hydrolysis probe.
- C.
  
  Be performed at single temperature with no specialized instrumentation required.
- D.
  
  Be interpreted as a plus / minus result or as a quantitative result.
6.

Choose the statement that correctly finishes the sentence: “A PCR reaction that contains only one copy of the target sequence (1 copy /reaction)…
- A.
  
  Is typically amplified in a highly repeatable manner.”
- B.
  
  May amplify but is detection is not likely to be highly repeatable.”
- C.
  
  Can be precisely and accurately quantified using quantitative PCR.”
- D.
  
  All of the above.
7.

RNA is copied into complementary DNA (cDNA) by:
- A.
  
  Taq DNA polymerase.
- B.
  
  RNA polymerase II.
- C.
  
  Reverse transcriptase.
- D.
  
  Uracil-N-Glycosylase.
8.

The reverse transcriptase reaction can be primed by:
- A.
  
  Target sequence specific primers.
- B.
  
  Random hexamers.
- C.
  
  Oligo dT primers.
- D.
  
  All of the above.
9.

Select the application of RT-PCR.
- A.
  
  Drug therapy efficacy.
- B.
  
  Verification of microarray results.
- C.
  
  Allelic discrimination assays or SNP genotyping.
- D.
  
  Quantitative mRNA expression studies.
- E.
  
  DNA replication efficiency.
10.

Human errors limit the accuracy of the real-time PCR results:
- A.
  
  Incorrect data analysis.
- B.
  
  Improper assay development.
- C.
  
  Destroy of DNA.
- D.
  
  Unwarranted conclusions.