Quiz: Real-Time PCR

10 Questions | Total Attempts: 1323

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Quiz: Real-Time PCR

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Questions and Answers
  • 1. 
    Who received the Nobel Prize in Chemistry in 1993 for inventing PCR?
    • A. 

      David Baltimore

    • B. 

      Kary Mullis

    • C. 

      Robert Weinberg

    • D. 

      John Nash

  • 2. 
    What does the acronym PCR stand for?
    • A. 

      Polymerase Chain Reaction

    • B. 

      Polymerase Copying Reaction

    • C. 

      Polymerase Copied Repeats

    • D. 

      Polymerase Chain Restoration

  • 3. 
    This is the sequence order of RT-PCR: 1. Denature 2. Anneal 3. Detect 4. Extend
    • A. 

      True

    • B. 

      False

  • 4. 
    Nucleotide triphosphates (dNTPs) are added to the growing DNA strand during the _______________ phase. 
    • A. 

      Extension/elongation

    • B. 

      Annealing

    • C. 

      Denaturation/separation

    • D. 

      Preparation

  • 5. 
    • A. 

      Incorporate dyes that bind double-stranded DNA.

    • B. 

      Incorporate an internal hydrolysis probe.

    • C. 

      Be performed at single temperature with no specialized instrumentation required.

    • D. 

      Be interpreted as a plus / minus result or as a quantitative result.

  • 6. 
    Choose the statement that correctly finishes the sentence: “A PCR reaction that contains only one copy of the target sequence (1 copy /reaction)…
    • A. 

      Is typically amplified in a highly repeatable manner.”

    • B. 

      May amplify but is detection is not likely to be highly repeatable.”

    • C. 

      Can be precisely and accurately quantified using quantitative PCR.”

    • D. 

      All of the above.

  • 7. 
    RNA is copied into complementary DNA (cDNA) by:
    • A. 

      Taq DNA polymerase.

    • B. 

      RNA polymerase II.

    • C. 

      Reverse transcriptase.

    • D. 

      Uracil-N-Glycosylase.

  • 8. 
    The reverse transcriptase reaction can be primed by: 
    • A. 

      Target sequence specific primers.

    • B. 

      Random hexamers.

    • C. 

      Oligo dT primers.

    • D. 

      All of the above.

  • 9. 
    Select the application of RT-PCR.
    • A. 

      Drug therapy efficacy.

    • B. 

      Verification of microarray results.

    • C. 

      Allelic discrimination assays or SNP genotyping.

    • D. 

      Quantitative mRNA expression studies.

    • E. 

      DNA replication efficiency.

  • 10. 
    Human errors limit the accuracy of the real-time PCR results: 
    • A. 

      Incorrect data analysis.

    • B. 

      Improper assay development.

    • C. 

      Destroy of DNA.

    • D. 

      Unwarranted conclusions.