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David Baltimore
Kary Mullis
Robert Weinberg
John Nash
Extension/elongation
Annealing
Denaturation/separation
Preparation
Polymerase Chain Reaction
Polymerase Copying Reaction
Polymerase Copied Repeats
Polymerase Chain Restoration
True
False
Incorporate dyes that bind double-stranded DNA.
Incorporate an internal hydrolysis probe.
Be performed at a single temperature with no specialized instrumentation required.
Be interpreted as a plus/minus result or as a quantitative result.
Is typically amplified in a highly repeatable manner.”
May amplify but is detection is not likely to be highly repeatable.”
Can be precisely and accurately quantified using quantitative PCR.”
All of the above.
Taq DNA polymerase.
RNA polymerase II.
Reverse transcriptase.
Uracil-N-Glycosylase.
Target sequence-specific primers.
Random hexamers.
Oligo dT primers.
All of the above.
Drug therapy efficacy.
Verification of microarray results.
Allelic discrimination assays or SNP genotyping.
Quantitative mRNA expression studies.
DNA replication efficiency.
Incorrect data analysis.
Improper assay development.
DNA destroyed
Unwarranted conclusions.
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