Molecular Monitoring: Real-Time PCR Quiz

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Molecular Monitoring: Real-Time PCR Quiz - Quiz

Here is a very amazing quiz that we have made for you about Real-Time PCR. This quiz is here to test how well you are aware of Real-time PCR. Do you know well enough to score well on this quiz? If yes, then beat this quiz with an 80 percent score.

Dive deep into the intricate world of real-time PCR with the "Molecular Monitoring: Real-Time PCR Quiz." Real-time PCR, or qPCR, has revolutionized molecular biology, allowing scientists to monitor the amplification of DNA in real time. This quiz is designed to test your understanding of the principles, techniques, and applications Read moreof real-time PCR.

Navigate through questions that explore the basics of qPCR, from the choice of fluorescent dyes to the intricacies of primer design. Delve into the significance of amplification plots and melting curve analysis in molecular monitoring. Challenge yourself with queries on quantification methods, data analysis, and the advantages of real-time PCR over traditional PCR.

Whether you're a seasoned molecular biologist or a budding researcher, this quiz offers a comprehensive exploration of real-time PCR concepts. Test your knowledge and gain insights into how this powerful technique is employed in various scientific disciplines. This will also sharpen your knowledge on this topic. Best of luck!


Questions and Answers
  • 1. 

    In 1993, _________ received the Nobel Prize in Chemistry for inventing PCR?

    • A.

      David Baltimore

    • B.

      Kary Mullis

    • C.

      Robert Weinberg

    • D.

      John Nash

    Correct Answer
    B. Kary Mullis
    Explanation
    Kary Mullis received the Nobel Prize in Chemistry in 1993 for inventing PCR, which stands for Polymerase Chain Reaction. PCR is a technique used in molecular biology to amplify a specific segment of DNA. Mullis' invention revolutionized the field of genetics and had a profound impact on various scientific disciplines. His work has enabled advancements in DNA sequencing, genetic testing, forensic analysis, and disease diagnosis, making him a deserving recipient of the Nobel Prize in Chemistry.

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  • 2. 

     During which phase are Nucleotide triphosphates (dNTPs) added to the growing DNA strand? 

    • A.

      Extension/elongation

    • B.

      Annealing

    • C.

      Denaturation/separation

    • D.

      Preparation

    Correct Answer
    A. Extension/elongation
    Explanation
    During the extension/elongation phase of DNA replication, nucleotide triphosphates (dNTPs) are added to the growing DNA strand. This phase occurs after the denaturation/separation phase, where the DNA strands are separated, and before the annealing phase, where the primers bind to the template DNA. The extension/elongation phase involves the DNA polymerase enzyme synthesizing a new DNA strand by adding complementary nucleotides to the template strand. This results in the elongation and replication of the DNA molecule. Therefore, the correct answer is extension/elongation.

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  • 3. 

    The acronym PCR stands for

    • A.

      Polymerase Chain Reaction

    • B.

      Polymerase Copying Reaction

    • C.

      Polymerase Copied Repeats

    • D.

      Polymerase Chain Restoration

    Correct Answer
    A. Polymerase Chain Reaction
    Explanation
    PCR stands for Polymerase Chain Reaction. This technique is used to amplify a specific segment of DNA, making multiple copies of it. It involves a series of heating and cooling cycles that allow DNA to denature, anneal primers, and extend the DNA chain using a heat-stable DNA polymerase enzyme. PCR is widely used in various fields such as genetics, forensics, and medical research for applications such as DNA sequencing, diagnosis of genetic diseases, and identification of pathogens.

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  • 4. 

    The sequence order of RT-PCR is: 1. Denature 2. Anneal 3. Detect 4. Extend

    • A.

      True

    • B.

      False

    Correct Answer
    B. False
    Explanation
    The correct answer is False. The sequence order of RT-PCR is as follows: 1. Denature, 2. Anneal, 3. Extend, and 4. Detect. In the denature step, the double-stranded DNA template is heated to separate the strands. In the anneal step, the primers bind to their complementary sequences on the template. In the extend step, the enzyme synthesizes new DNA strands using the primers as a starting point. Finally, in the detect step, the amplified DNA is detected using a fluorescent probe or other detection method.

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  • 5. 

    Which of these is/are the property of real-time PCR assays?

    • A.

      Incorporate dyes that bind double-stranded DNA.

    • B.

      Incorporate an internal hydrolysis probe.

    • C.

      Be performed at a single temperature with no specialized instrumentation required.

    • D.

      Be interpreted as a plus/minus result or as a quantitative result.

    Correct Answer(s)
    A. Incorporate dyes that bind double-stranded DNA.
    B. Incorporate an internal hydrolysis probe.
    D. Be interpreted as a plus/minus result or as a quantitative result.
    Explanation
    Real-time PCR assays incorporate dyes that bind double-stranded DNA, which allows for the detection and quantification of the DNA amplification in real-time. They also incorporate an internal hydrolysis probe, which further enhances the specificity and accuracy of the assay. These assays can be performed at a single temperature, eliminating the need for specialized instrumentation. The results of real-time PCR assays can be interpreted as a plus/minus result or as a quantitative result, providing flexibility in data analysis.

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  • 6. 

    “A PCR reaction that has only one copy of the target sequence (1 copy /reaction)…

    • A.

      Is typically amplified in a highly repeatable manner.”

    • B.

      May amplify but is detection is not likely to be highly repeatable.”

    • C.

      Can be precisely and accurately quantified using quantitative PCR.”

    • D.

      All of the above.

    Correct Answer
    B. May amplify but is detection is not likely to be highly repeatable.”
    Explanation
    The correct answer is "may amplify but its detection is not likely to be highly repeatable." This is because when there is only one copy of the target sequence in a PCR reaction, it may still undergo amplification, but the detection of this amplified product may not be consistent or reliable. This is because the low starting copy number makes it more prone to errors and variations during the amplification process, leading to less reproducible results.

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  • 7. 

    By what is RNA copied into complementary DNA (cDNA)?

    • A.

      Taq DNA polymerase.

    • B.

      RNA polymerase II.

    • C.

      Reverse transcriptase.

    • D.

      Uracil-N-Glycosylase.

    Correct Answer
    C. Reverse transcriptase.
    Explanation
    Reverse transcriptase is the correct answer because it is an enzyme that can synthesize a complementary DNA (cDNA) strand from an RNA template. This process is called reverse transcription and is commonly used in molecular biology research to study gene expression. Reverse transcriptase is derived from retroviruses and is capable of converting RNA into DNA, allowing researchers to study RNA molecules using DNA-based techniques.

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  • 8. 

    By what can the reverse transcriptase reaction be primed? 

    • A.

      Target sequence-specific primers.

    • B.

      Random hexamers.

    • C.

      Oligo dT primers.

    • D.

      All of the above.

    Correct Answer
    D. All of the above.
    Explanation
    The reverse transcriptase reaction can be primed by target sequence-specific primers, random hexamers, or oligo dT primers. Target sequence-specific primers are designed to anneal to a specific target sequence, providing a specific starting point for reverse transcription. Random hexamers are short, random sequences that can bind to any RNA molecule, allowing for the initiation of reverse transcription at multiple sites. Oligo dT primers are designed to bind to the polyadenine tail present on most eukaryotic mRNA molecules, providing a starting point for reverse transcription. Therefore, all of these primers can be used to prime the reverse transcriptase reaction.

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  • 9. 

    Check the boxes with the application of RT-PCR.

    • A.

      Drug therapy efficacy.

    • B.

      Verification of microarray results.

    • C.

      Allelic discrimination assays or SNP genotyping.

    • D.

      Quantitative mRNA expression studies.

    • E.

      DNA replication efficiency.

    Correct Answer(s)
    A. Drug therapy efficacy.
    B. Verification of microarray results.
    C. Allelic discrimination assays or SNP genotyping.
    D. Quantitative mRNA expression studies.
    Explanation
    RT-PCR (Reverse Transcription Polymerase Chain Reaction) is a molecular biology technique used to amplify and detect specific RNA sequences. It is commonly used in various applications, including drug therapy efficacy, verification of microarray results, allelic discrimination assays or SNP genotyping, and quantitative mRNA expression studies. In drug therapy efficacy, RT-PCR can be used to determine the effectiveness of a drug by measuring the expression levels of specific target genes. It can also be used to validate the results obtained from microarray experiments, which is a high-throughput method for gene expression analysis. Additionally, RT-PCR can be utilized for allelic discrimination assays or SNP genotyping to identify genetic variations. Lastly, it is commonly used for quantitative mRNA expression studies to measure the expression levels of specific genes.

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  • 10. 

    Which human errors limit the accuracy of the real-time PCR results: 

    • A.

      Incorrect data analysis.

    • B.

      Improper assay development.

    • C.

      DNA destroyed

    • D.

      Unwarranted conclusions.

    Correct Answer(s)
    A. Incorrect data analysis.
    B. Improper assay development.
    D. Unwarranted conclusions.
    Explanation
    The accuracy of real-time PCR results can be limited by several human errors. Incorrect data analysis can lead to misinterpretation of the results and incorrect conclusions. Improper assay development can result in inaccurate measurements and unreliable data. Unwarranted conclusions can be drawn when there is a lack of proper analysis and interpretation of the results. These errors can all contribute to inaccuracies in the real-time PCR results, affecting the overall reliability and validity of the findings.

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  • Current Version
  • Nov 24, 2023
    Quiz Edited by
    ProProfs Editorial Team
  • Dec 05, 2016
    Quiz Created by
    Xinhuey94vv
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