Quiz: Real-Time PCR

10 Questions | By Xinhuey94vv | Updated: Mar 22, 2022 | Attempts: 12634

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Here is a very amazing quiz that we have made for you about Real-Time PCR. This quiz is here to test how well you are aware of Real-time PCR. Do you know well enough to score well on this quiz? If yes, then beat this quiz with an 80 percent score. This will also sharpen your knowledge on this topic. Comment the score you get after the test. Best of luck!

Questions and Answers

1.

In 1993, _________ received the Nobel Prize in Chemistry for inventing PCR?
- A.
  
  David Baltimore
- B.
  
  Kary Mullis
- C.
  
  Robert Weinberg
- D.
  
  John Nash
2.

During which phase are Nucleotide triphosphates (dNTPs) added to the growing DNA strand?
- A.
  
  Extension/elongation
- B.
  
  Annealing
- C.
  
  Denaturation/separation
- D.
  
  Preparation
3.

The acronym PCR stand for
- A.
  
  Polymerase Chain Reaction
- B.
  
  Polymerase Copying Reaction
- C.
  
  Polymerase Copied Repeats
- D.
  
  Polymerase Chain Restoration
4.

The sequence order of RT-PCR is: 1. Denature 2. Anneal 3. Detect 4. Extend
- A.
  
  True
- B.
  
  False
5.

Which of these is/are the property of real-time PCR assays?
- A.
  
  Incorporate dyes that bind double-stranded DNA.
- B.
  
  Incorporate an internal hydrolysis probe.
- C.
  
  Be performed at a single temperature with no specialized instrumentation required.
- D.
  
  Be interpreted as a plus/minus result or as a quantitative result.
6.

“A PCR reaction that has only one copy of the target sequence (1 copy /reaction)…
- A.
  
  Is typically amplified in a highly repeatable manner.”
- B.
  
  May amplify but is detection is not likely to be highly repeatable.”
- C.
  
  Can be precisely and accurately quantified using quantitative PCR.”
- D.
  
  All of the above.
7.

By what is RNA copied into complementary DNA (cDNA)?
- A.
  
  Taq DNA polymerase.
- B.
  
  RNA polymerase II.
- C.
  
  Reverse transcriptase.
- D.
  
  Uracil-N-Glycosylase.
8.

By what can the reverse transcriptase reaction be primed?
- A.
  
  Target sequence-specific primers.
- B.
  
  Random hexamers.
- C.
  
  Oligo dT primers.
- D.
  
  All of the above.
9.

Check the boxes with the application of RT-PCR.
- A.
  
  Drug therapy efficacy.
- B.
  
  Verification of microarray results.
- C.
  
  Allelic discrimination assays or SNP genotyping.
- D.
  
  Quantitative mRNA expression studies.
- E.
  
  DNA replication efficiency.
10.

Which human errors limit the accuracy of the real-time PCR results:
- A.
  
  Incorrect data analysis.
- B.
  
  Improper assay development.
- C.
  
  DNA destroyed
- D.
  
  Unwarranted conclusions.