Quiz | Real-time Pcr
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After read through about real-time PCR in our website, we would like to invite you to take this quiz!What after reading is understanding! Take this quiz now to see how far you understand about real-time PCR technique!Start my 10 multiple choice questions. All the best!Sincerely from,The authors (Ching Xin Huey & Yeo Zhin Leng)
- 1.Who received the Nobel Prize in Chemistry in 1993 for inventing PCR?
- A.
David Baltimore
- B.
Kary Mullis
- C.
Robert Weinberg
- D.
John Nash
- 2.What does the acronym PCR stand for?
- A.
Polymerase Chain Reaction
- B.
Polymerase Copying Reaction
- C.
Polymerase Copied Repeats
- D.
Polymerase Chain Restoration
- 3.This is the sequence order of RT-PCR.1. Denature2. Anneal3. Detect4. Extend
- A.
True
- B.
False
- 4.Nucleotide triphosphates (dNTPs) are added to the growing DNA strand during the _______________ phase.
- A.
Extension/elongation
- B.
Annealing
- C.
Denaturation/separation
- D.
Preparation
- 5.
- A.
Incorporate dyes that bind double-stranded DNA
- B.
Incorporate an internal hydrolysis probe
- C.
Be performed at single temperature with no specialized instrumentation required
- D.
Be interpreted as a plus / minus result or as a quantitative result
- 6.
- A.
Is typically amplified in a highly repeatable manner”
- B.
May amplify but is detection is not likely to be highly repeatable”
- C.
Can be precisely and accurately quantified using quantitative PCR”
- D.
All of the above
- 7.RNA is copied into complementary DNA (cDNA) by:
- A.
Taq DNA polymerase
- B.
RNA polymerase II
- C.
Reverse transcriptase
- D.
Uracil-N-Glycosylase
- 8.The reverse transcriptase reaction can be primed by
- A.
Target sequence specific primers
- B.
Random hexamers
- C.
Oligo dT primers
- D.
All of the above
- 9.Select the application of RT-PCR.
- A.
Drug therapy efficacy
- B.
Verification of microarray results
- C.
Allelic discrimination assays or SNP genotyping
- D.
Quantitative mRNA expression studies
- E.
DNA replication efficiency
- 10.Human errors limits the accuracy of the real-time PCR results:
- A.
Incorrect data analysis
- B.
improper assay development
- C.
Destroy of DNA
- D.
Unwarranted conclusions
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