Quiz: Real-Time PCR
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- 1.Who received the Nobel Prize in Chemistry in 1993 for inventing PCR?
- A.
David Baltimore
- B.
Kary Mullis
- C.
Robert Weinberg
- D.
John Nash
- 2.What does the acronym PCR stand for?
- A.
Polymerase Chain Reaction
- B.
Polymerase Copying Reaction
- C.
Polymerase Copied Repeats
- D.
Polymerase Chain Restoration
- 3.This is the sequence order of RT-PCR: 1. Denature 2. Anneal 3. Detect 4. Extend
- A.
True
- B.
False
- 4.Nucleotide triphosphates (dNTPs) are added to the growing DNA strand during the _______________ phase.
- A.
Extension/elongation
- B.
Annealing
- C.
Denaturation/separation
- D.
Preparation
- 5.
- A.
Incorporate dyes that bind double-stranded DNA.
- B.
Incorporate an internal hydrolysis probe.
- C.
Be performed at single temperature with no specialized instrumentation required.
- D.
Be interpreted as a plus / minus result or as a quantitative result.
- 6.Choose the statement that correctly finishes the sentence: “A PCR reaction that contains only one copy of the target sequence (1 copy /reaction)…
- A.
Is typically amplified in a highly repeatable manner.”
- B.
May amplify but is detection is not likely to be highly repeatable.”
- C.
Can be precisely and accurately quantified using quantitative PCR.”
- D.
All of the above.
- 7.RNA is copied into complementary DNA (cDNA) by:
- A.
Taq DNA polymerase.
- B.
RNA polymerase II.
- C.
Reverse transcriptase.
- D.
Uracil-N-Glycosylase.
- 8.The reverse transcriptase reaction can be primed by:
- A.
Target sequence specific primers.
- B.
Random hexamers.
- C.
Oligo dT primers.
- D.
All of the above.
- 9.Select the application of RT-PCR.
- A.
Drug therapy efficacy.
- B.
Verification of microarray results.
- C.
Allelic discrimination assays or SNP genotyping.
- D.
Quantitative mRNA expression studies.
- E.
DNA replication efficiency.
- 10.Human errors limit the accuracy of the real-time PCR results:
- A.
Incorrect data analysis.
- B.
Improper assay development.
- C.
Destroy of DNA.
- D.
Unwarranted conclusions.
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