Electrophoresis Blood Quiz Questions
- 1.While synthesizing a new blue pigment, a chemist noticesthat the new compound congregates between an aqueous(water) environment and a hydrophobic environment.When added to a mixture of oil and water, the pigmentcreates a blue ring around the droplets of oil. Which of thefollowing statements best describes this new pigment?
- A.
The pigment is amphipathic, having polar and nonpolar regions.
- B.
The pigment is probably hydrophobic and is attempting to bond with the oil.
- C.
The pigment is hydrophilic and will not form hydrophobic bonds with the oil.
- D.
The pigment is a polar molecule and is forming hydrogen bonds with both the water and oil molecules.
- E.
The pigment is neither polar or nonpolar, but is apolar.
- 2.An enzyme synthesized in the laboratory is found to havelittle activity when compared to the same enzyme extractedfrom cell culture. Both enzymes were examined and haveidentical amino acid composition. What is the bestexplanation for the lack of activity of the synthesizedenzyme?
- A.
The synthetic enzyme was not made of amino acids
- B.
Denaturation of the synthesized enzyme was not complete.
- C.
The synthesized enzyme was not folded correctly
- D.
The van der Waals radius was altered during laboratory synthesis.
- E.
The ATP required for self-assembly was present in the cell extract but not in the laboratory synthesis.
- 3.Why is the decrease in the surface area/volume ratio as cellsincrease in size a problem for cells?
- A.
The ability of a cell to obtain a sufficient amount of nutrients from the environment is hampered
- B.
The ability of a cell to get rid of wastes at a high enough rate is compromised by the lower ratio.
- C.
The ability of a cell to get rid of nutrients to the environment is hampered
- D.
The ability of a cell to build up wastes at a high enough rate is compromised by the lower ratio.
- E.
A and b
- 4.Membrane proteins may function in
- A.
Temperature control.
- B.
Information storage
- C.
Amphipathic modification
- D.
Protein manufacture.
- E.
Receiving extracellular signals.
- 5.Disulfide bonds are often found to stabilize which of thefollowing levels of protein structure?
- A.
Secondary
- B.
Primary
- C.
Tertiary
- D.
All of the above
- E.
None of the above
- 6.Which of the following is NOT true of phospholipids?
- A.
Serine is a molecule that may be part of a phosphoglyceride.
- B.
Phospholipids are important in membrane structure.
- C.
Phosphatidic acid contains two fatty acids and a phosphate group.
- D.
Sphingolipids are the predominant phospholipid in membranes.
- E.
Phospholipids are amphipathic.
- 7.The term amphoteric describes the characteristic of somemolecules that have ________.
- A.
Only a single polar region
- B.
Two polar regions
- C.
Can donate or accept electrons
- D.
Can accept or donate protons
- E.
Both a polar and a nonpolar region
- 8.Which of the following is NOT a function of membranes?
- A.
Define cell boundaries
- B.
Regulation of transport
- C.
Compartmentalization
- D.
Cell-cell communication
- E.
Information storage
- 9.HCompounds that react with free hydrogen or hydroxyl ions,thereby resisting changes in pH, _______.
- A.
Are called buffers
- B.
Protect cells and organisms from fluctuations in salinity
- C.
Protect cells and organisms from pH stability
- D.
A and b
- E.
A and c
- 10.With respect to the outer and inner faces of the lipid bilayerof the cell membrane, the composition of lipids is
- A.
A mirror image
- B.
Asymmetrical.
- C.
Not identical, but symmetrical.
- D.
Highly random and varies throughout the cell.
- E.
Identical.
- 11.Which of the following is NOT a fundamental property ofcarbon? 1
- A.
Carbon-containing molecules form stereoisomers.
- B.
Carbon-containing molecules are stable.
- C.
Carbon-containing molecules are diverse.
- D.
Carbon atoms are most likely to form ionic bonds with one another.
- E.
Carbon has a valence of 4.
- 12.Why is a selectively permeable membrane so important toliving things?
- A.
The membrane may absorb several times its weight in cholesterol.
- B.
It provides a good barrier between the inside and outside of the cell.
- C.
It allows cells to attach to adjacent tissues.
- D.
Proteins will avoid a selectively permeable membrane.
- E.
All of the above
- 13.An organism is isolated from a glacier, where it has beenobserved to be thriving. Based on your knowledge of theplasma membrane, you would expect to find apredominance of
- A.
Equal amounts of saturated and unsaturated fatty acids, as long as the chain was close to 12 carbons in length.
- B.
Largely saturated fatty acids.
- C.
Cell wall to define the cell, as the cell membrane would not be present.
- D.
Largely unsaturated fatty acids.
- E.
None of the above
- 14.Based on your knowledge of the chemistry of the cellmembrane, which of the following compounds would youcouple with a new drug in order to achieve better uptake bycells?
- A.
Several mannose-6-phosphate monomers
- B.
Carbohydrates
- C.
Any hydrophilic substance
- D.
Polar amino acids
- E.
Lipids
- 15.Cholesterol
- A.
Destabilizes the cell membrane.
- B.
Is found only in plants.
- C.
Intercalates between the fatty acid chain within the bilayer.
- D.
Is found in abundance in prokaryotic cell membranes.
- E.
Hincreases permeability for small cations.
- 16.Based on the following amino acid sequence, whichregion(s) is/are hydrophobic?1[Lysine-Lysine-Glutamine] - 2[Alanine-Glycine-Tryptophan-Valine] - 3[Tyrosine-Cysteine-Serine-Arginine] - 4[Histidine-Glutamic acid-Cysteine-Tryptophane]
- A.
Region 1
- B.
Region 3
- C.
Region 2
- D.
Region 4
- 17.With respect to the outer and inner faces of the lipid bilayerof the cell membrane, the composition of proteins is
- A.
Asymmetrical.
- B.
Identical.
- C.
Not identical, but symmetrical.
- D.
A mirror image.
- E.
Highly random and varies throughout the cell.
- 18.The technique that allows various proteins to bedifferentiated based on size, once the proteins have beendenatured, is
- A.
Ferritin conjugated lectins.
- B.
SDS-PAGE.
- C.
Photobleaching.
- D.
Liposome formation.
- E.
The freeze fracture technique.
- 19.Which amino acid is most likely to be found in the core ofa protein?
- A.
Phenylalanine
- B.
Asparagine
- C.
Serine
- D.
Threonine
- E.
Glutamic acid
- 20.Chaperones were first identified as_______________proteins and they informationfor protein folding. They ______________ interactions tooccur
- A.
Heat-shock, do. Hydrophobic
- B.
Heat- shock, do. Hydrophilic
- C.
Heat shock, do not. Hydrophilic
- D.
Heat shock, do not. Hydrophobic
- 21.The á-helix is characterized by orientation of ______ themolecular axis.
- A.
H bonds perpendicular to
- B.
Ionic bonds parallel to
- C.
Ionic bonds perpendicular to
- D.
Peptide bonds perpendicular to
- E.
H bonds parallel to
- 22.What level of structure in proteins is held together byintramolecular R group interactions?
- A.
Primary structure
- B.
Secondary structure
- C.
Tertiary structure
- D.
Quaternary structure
- E.
C and d
- 23.What kind of protein is associated with either theextracellular or cytoplasmic surface using a lipid tail?
- A.
Integral membrane proteins
- B.
Peripheral proteins
- C.
GPI-anchor proteins
- D.
Chaperones
- 24.What kind of proteins can be obtained by treating cells withhigh-salt solutions?
- A.
GPI-peripheral proteins
- B.
Peripheral proteins
- C.
Transmembrane proteins
- D.
A and c
- E.
B and c
- 25.While culturing some cells, you lower the temperature ofthe culture. What happens immediately to the membranefluidity?
- A.
Nothing happens
- B.
The membrane becomes more fluid
- C.
The membrane becomes less fulid
- D.
The membrane fluidity fluctuates back and forth from high to low fluidity
- E.
The membrane fluidity fluctuates back and forth from low to high
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