A Quiz On Microbial Growth

1. Aseptic technique refers to carry out a procedure under controlled conditions in a manner that will minimize the chance of contamination.

True

False

Aseptic technique is a method used to perform procedures in a way that reduces the risk of contamination. This means that the procedure is carried out under controlled conditions, such as using sterile equipment and maintaining a sterile environment. By following aseptic technique, the chances of introducing harmful microorganisms into the procedure are minimized, ensuring a safer and more effective outcome. Therefore, the statement that aseptic technique refers to carrying out a procedure under controlled conditions to minimize contamination is true.

Explanation

Aseptic technique is a method used to perform procedures in a way that reduces the risk of contamination. This means that the procedure is carried out under controlled conditions, such as using sterile equipment and maintaining a sterile environment. By following aseptic technique, the chances of introducing harmful microorganisms into the procedure are minimized, ensuring a safer and more effective outcome. Therefore, the statement that aseptic technique refers to carrying out a procedure under controlled conditions to minimize contamination is true.

2. Chemical growth requirements essential for microorganism growth is.

Water only

Environmental waste.

Product from other bacterial waste

Water,carbon,oxygen,phosphorus,Nitrogen,sulfur and trace element

Basically,microorganism required water,carbon,oxygen,phosphorus,Nitrogen,sulfur and trace element in order for them to grow on a particular environment.

Explanation

Basically,microorganism required water,carbon,oxygen,phosphorus,Nitrogen,sulfur and trace element in order for them to grow on a particular environment.

3. Selective media contain agents that inhibit the growth of certain bacteria while permitting the growth of other.

True

False

Selective media are specifically formulated to promote the growth of certain types of bacteria while inhibiting the growth of others. These media contain substances such as antibiotics, dyes, or chemicals that create an environment unfavorable for the growth of certain bacteria. By selectively inhibiting the growth of unwanted bacteria, selective media allow for the isolation and identification of specific bacterial species or groups. Therefore, the statement that selective media contain agents that inhibit the growth of certain bacteria while permitting the growth of others is true.

Explanation

Selective media are specifically formulated to promote the growth of certain types of bacteria while inhibiting the growth of others. These media contain substances such as antibiotics, dyes, or chemicals that create an environment unfavorable for the growth of certain bacteria. By selectively inhibiting the growth of unwanted bacteria, selective media allow for the isolation and identification of specific bacterial species or groups. Therefore, the statement that selective media contain agents that inhibit the growth of certain bacteria while permitting the growth of others is true.

4. Growth are referring to the number of cells, not the size.

True

False

The increasing of bacterial size does not show the increasing of bacterial growth.

Explanation

The increasing of bacterial size does not show the increasing of bacterial growth.

5. Psychrophiles are those bacteria that able to grow under........

0 – 20ºC

25 – 40ºC

50 – 60 ºC

70 – 110 ºC

Mesophiles : 25 – 40ºC
Moderate thermophiles : 50 – 60 ºC
Extreme thremophiles : 70 – 110 ºC

Explanation

Mesophiles : 25 – 40ºC
Moderate thermophiles : 50 – 60 ºC
Extreme thremophiles : 70 – 110 ºC

6. Bacteria mainly grow via binary fission. Some can grow by

Budding only

Spores only

Budding,spores, or fragmentation

None of the above

Some bacteria able to grow via budding,spores or fragmentation

Explanation

Some bacteria able to grow via budding,spores or fragmentation

7. What are the factor that shifting the phase to the decline phase ?

Condition in the medium become less but cell still have the ability to divide rapidly

Condition in the medium become less and cell lose their ability to divide

Number of live cells increases at a logarithmic rate

Condition in the medium become less because there are too many competition to get the limited nutrient and on top of that, many side product produce by the microorganism cause the condition become unfavorable to grow.So,when this occur,cell will lose their ability to divide and eventually the organism will die.

Explanation

Condition in the medium become less because there are too many competition to get the limited nutrient and on top of that, many side product produce by the microorganism cause the condition become unfavorable to grow.So,when this occur,cell will lose their ability to divide and eventually the organism will die.

8. Tolerance referring to the survival of bacteria under conditions in which they cannot grow.

True

False

Tolerance refers to the ability of bacteria to survive under conditions in which they cannot grow. This means that even though the bacteria may not be able to reproduce or thrive, they are still able to survive in these unfavorable conditions. Therefore, the statement "Tolerance referring to the survival of bacteria under conditions in which they cannot grow" is true.

Explanation

Tolerance refers to the ability of bacteria to survive under conditions in which they cannot grow. This means that even though the bacteria may not be able to reproduce or thrive, they are still able to survive in these unfavorable conditions. Therefore, the statement "Tolerance referring to the survival of bacteria under conditions in which they cannot grow" is true.

9. Mac Conkey's agar is a differential media that contains lactose as substrate and neutral red as an indicator. This agar is used to

Differentiate lactose fermenters from non-lactose fermenters

Differentiate sucrose fermenters with non-sucrose fermenters bacteria.

Differentiate glucose fermenters with non - glucose fermenters

Identify the bacteria that able to degrade peptone into amino acid.

Bacteria that able to ferment lactose in Macconkey agar plate will give red colour on the growth.

Explanation

Bacteria that able to ferment lactose in Macconkey agar plate will give red colour on the growth.

10. Osmosis is one of the processes in passive transportation. What is the movement of the water when there's high solute concentration outside the cell?

Water move from inside to outside cell

Water move from outside to inside cell

There's no net movement of water molecules.

None of the above

Osmosis is the net movement of water molecules from the high concentration gradient to lower concentration gradient ?

Explanation

Osmosis is the net movement of water molecules from the high concentration gradient to lower concentration gradient ?

11. Blood agar is unable to cultivate the fastidious microorganisms

True

False

Blood agar able to cultivate all the fastidious microorganism

Explanation

Blood agar able to cultivate all the fastidious microorganism

12. Microorganism obtained their carbon source from the carbon dioxide are called?

Chemotroph

Autotroph

Heterotroph

Saprophyte

Autotroph obtain their carbon source from the carbon dioxide.

Explanation

Autotroph obtain their carbon source from the carbon dioxide.

13. Organisms do not increase significantly in number and produce large quantities of energy in the form of ATP. Which of the phase in the exponential growth curve explains these statements?

Lag phase

Stationary phase

Log phase

Decline phase

In lag phase, organisms do not increase significantly in number and produce large quantities of energy in the form of ATP

Explanation

In lag phase, organisms do not increase significantly in number and produce large quantities of energy in the form of ATP

14. Microorganisms obtain their carbon source in many different ways. Those that able to get the carbon source from the organic compound are called?

Autotroph

Chemotroph

Heterotroph

Organotroph

Heterotroph get their carbon source from the organic compound.

Explanation

Heterotroph get their carbon source from the organic compound.

15. Which is the following the main requirement(s) for the bacteria to grow?

Oxygen requirement

Nutrient requirement

Oxygen and nutrient requirement

Physical and chemical requirement

physical and chemical requirement are the basic/main requirement required by the bacteria to grow whereas the other is just an example of physical and chemical requirement.

Explanation

physical and chemical requirement are the basic/main requirement required by the bacteria to grow whereas the other is just an example of physical and chemical requirement.