Molecular Biology (Lectures 15-17)

25 Questions | Total Attempts: 113

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Molecular Biology Quizzes & Trivia

Questions and Answers
  • 1. 
    What is the process in which fragments of DNA from each gene are "spotted" and immobilized on a glass microscope slide?
    • A. 

      DNA Microarray

    • B. 

      DNA Nanoarray

    • C. 

      SDS-PAGE

    • D. 

      2-D Gel Electrophoresis

  • 2. 
    Point mutation caused by UV light
  • 3. 
    Point Mutation that adds C-H alkyl group to N or O atoms.
  • 4. 
    Point mutation that causes transition mutations of C-G to T-A, by a mechanism where the Amino group spontaneously reacts with H2O to add O and generate ammonium (NH4+) Is ______________ and occurs roughly once every ________ base pairs per cell per day, and C-G is changed to _ - _ bp, then _-_ replicates:  T-A (mutation) and C-G (wild type)
  • 5. 
    Point Mutation in which incorrect bases are added by DNA polymerase, are not corrected by the 3'-5' exonuclease proofreading, and fall into the categories of "transversion" and "transition" mutations.
  • 6. 
    Removes purine bases, forming abasic sites, at a frequency of roughly __________ purines per cell per day
  • 7. 
    Enzyme found in plants, many bacteria, and some animals, that repairs thymine dimers, directly.
  • 8. 
    Which of the folowing are true about excision repair systems?
    • A. 

      Appear to repair the majority of mutations

    • B. 

      Appear to repair relatively few mutations

    • C. 

      Multiple, redundant types

    • D. 

      Only one known version

    • E. 

      Each deals with different kinds of point mutations

  • 9. 
    The type of Excision repair that fixes a damaged, single base is known as....
  • 10. 
    How does base excision repair perform?
  • 11. 
    Check all that are true about nucleotide excision repair (NER)
    • A. 

      Fixes damaged bases

    • B. 

      10-12 nucleotides removed

    • C. 

      Involves uvr genes

    • D. 

      Fixes damaged, single base

  • 12. 
    Which excision repair system fixes mismatches?
  • 13. 
    In humans, what type of repair system manages thymine dimers?
  • 14. 
    People with ___________  _________ (XP) have defective NER systems- at least eight different genes for NER.
  • 15. 
    Which of the following are true about Xeroderma Pigmentosum (XP)?
    • A. 

      They cannot repair bulky DNA damage (T-dimers, abasic sites)

    • B. 

      1000 times more likely to develop skin cancer

    • C. 

      Sun (UV) increases cancer in people with XP (T-dimers)

  • 16. 
    What are the two types of NER forms in Eukaryotes?
  • 17. 
    Both types of NER activate the same __________  _________ repair proteins.
  • 18. 
    ___________   _____  when two non-pairing bases exist on complementary strands, involves mut genes in E.coli, and msh genes (mutS homologes) in eukaryotes.
  • 19. 
    Mismatch Repair Fixing Heteroduplex DNA: Problem: Which strand is correct and which strand has the mutated base? Solution: _________ of __ bases provides cues as to which strand is correct.
  • 20. 
    Some DNA in Bacteria is ____________ Helps in the repair of errors during replication.  "Old" DNA is ___________ (CH3) at A in 5'-GATC-3' About 10-20 min after replication, before "new" DNA strand is ___________ by "maintenance Methylase" Repair systems utilize this "Delay" to select old/correct strand (__________) from the new/defective strand (not  _________)
  • 21. 
    Photolyase is induced by....
  • 22. 
    Inducible repair system in E. coli (by mutagenic stress, T-dimers, etc.)
  • 23. 
    P53 is a ___________  _________  that activates: 1. ______________ 2. ______________ 3. ______________ (programmed cell death)
  • 24. 
    Inducible Repair SOS System in E. coli: DNA damage (________  _____) activates SOS Increases expression of ____ genes (error-prone bypass) Induction involves ______ that acts as protease to destroy _______ (transcription inhibitor)
  • 25. 
    ____-__________ ____ ______ (NHEJ) System: "Ligates" ends of broken linear DNA (chromosomes) Important for repairing DNA breakage (ionizing radiation)