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Advanced Microbiology Laboratory Techniques Quiz

Explore essential laboratory techniques in microbiology, focusing on practical skills and experimental methods. This educational tool is designed to enhance understanding of microbial cultures, staining procedures, and...

Questions: 10 | Attempts: 10 | Last updated: Aug 4, 2025

Sample Question 1
What is the Aerotolerance OCD formula for plate count dilution factor?

Aerotolerance Oxygen requirements for growthbrain heart infusion agar used Obligate Anaerobe: Growth on top onlyFacultative Org: growth Top-middle-bottomInf in a puncture wound: Aerotolerant bacteriaMicrophile requires a small concentration of oxygen for growth.

Average colony size multiplied by the dilution factor and volume plated

Total number of colonies multiplied by the dilution factor used

Number of colonies counted divided by the total volume of the original sample

Sample Question 2
What type of agar is Macconkey Agar?

Enrichment media

Indicator media

Blood agar

Plate count

Sample Question 3
What is the purpose of negative stain?

Aseptic Technique: a method used to avoid contamination of cultures and sterile materials in the laboratory.

Positive Stain: used to visualize structures that are difficult to see with a regular microscope.

Mannitol Salt Agar Selective and differential mediaUsed for DX Pathogenic S. AureusThe Org surrounded by yellow is considered positive The yellow represente the acidic waste products from the fermetation of a carbohydrate.The dark pink represente the alkaline wate products from the breakdown of preteins The selective agent is NaClThe differntial agent is MANNITOLBacteria that are capable to grow in this media are non-halophilic and gram negatives

Gram Staining: a technique used to differentiate bacterial species into two groups based on their cell wall composition.