Quiz Online 5 Chapter 6 Cloning Strategies

20 Questions

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Quiz Online 5 Chapter 6 Cloning Strategies

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Questions and Answers
  • 1. 
    The term ‘clone’ comes from the colonies of identical host cells produced during amplification of the cloned fragments.
    • A. 

      TRUE

    • B. 

      FALSE

  • 2. 
    Gene cloning is sometimes referred to as ‘molecular cloning’ to distinguish the process from the cloning of whole organisms.
    • A. 

      TRUE

    • B. 

      FALSE

  • 3. 
    Possible methods available for cloning procedure are the generation of DNA fragments, joining to a vector, and introducing the recombinant DNA into a host cell.
    • A. 

      TRUE

    • B. 

      FALSE

  • 4. 
    • A. 

      TRUE

    • B. 

      FALSE

  • 5. 
    Linkers are synthesised chemically and can be added to cDNA by blunt-end ligation.
    • A. 

      TRUE

    • B. 

      FALSE

  • 6. 
    • A. 

      An oligo(dT) primer

    • B. 

      Complementary DNA

    • C. 

      Reverse transcriptase

    • D. 

      Flush-ended molecule

    • E. 

      The 3-OH group

  • 7. 
    • A. 

      An oligo(dT) primer

    • B. 

      Complementary DNA

    • C. 

      Reverse transcriptase

    • D. 

      Flush-ended molecule

    • E. 

      The 3-OH group

  • 8. 
    • A. 

      Complementary DNA

    • B. 

      An oligo(dT) primer

    • C. 

      Reverse transcriptase

    • D. 

      Flush-ended molecule

    • E. 

      The 3-OH group

  • 9. 
    • A. 

      The 3-OH group

    • B. 

      Complementary DNA

    • C. 

      Flush-ended molecule

    • D. 

      An oligo(dT) primer

    • E. 

      Reverse transcriptase

  • 10. 
    • A. 

      The 3-OH group

    • B. 

      Complementary DNA

    • C. 

      Reverse transcriptase

    • D. 

      An oligo(dT) primer

    • E. 

      Flush-ended molecule

  • 11. 
    A colony of identical organisms; often used to describe a cell carrying a recombinant DNA fragment.
    • A. 

      Genomic library

    • B. 

      Homopolymer tailing

    • C. 

      Adaptor

    • D. 

      Linker

    • E. 

      Clone

  • 12. 
    The attachment of a sequence of identical nucleotides (e.g. AAAAA) to the end of a nucleic acid molecule, usually referring to the synthesis of single-stranded homopolymer extensions on the ends of a double-stranded DNA molecule.
    • A. 

      Genomic library

    • B. 

      Homopolymer tailing

    • C. 

      Adaptor

    • D. 

      Linker

    • E. 

      Clone

  • 13. 
    A collection of clones prepared from the mRNA of a given cell or tissue type, representing the genetic information expressed by such cells.
    • A. 

      Concatemer

    • B. 

      CDNA

    • C. 

      CDNA library

    • D. 

      Genomic library

  • 14. 
    A DNA molecule composed of a number of individual pieces joined together via cohesive ends.
    • A. 

      Concatemer

    • B. 

      CDNA

    • C. 

      CDNA library

    • D. 

      Genomic library

  • 15. 
    A collection of clones that together represent the entire genome of an organism.
    • A. 

      Concatemer

    • B. 

      CDNA

    • C. 

      CDNA library

    • D. 

      Genomic library

  • 16. 
    Artificial chromosomes such as YACs or BACs can be used to clone large fragments of DNA (in excess of several hundred kb).
    • A. 

      TRUE

    • B. 

      FALSE

  • 17. 
    Regulatory control of the expression of cloned genes can be achieved by selecting appropriate vector/host systems.
    • A. 

      TRUE

    • B. 

      FALSE

  • 18. 
    The use of a suitable promoter is a key element in achieving the expression of a gene by means of a cloned cDNA sequence.
    • A. 

      TRUE

    • B. 

      FALSE

  • 19. 
    Cloned cDNA can be used to express genes by enabling the synthesis of the protein in a suitable host system.
    • A. 

      TRUE

    • B. 

      FALSE

  • 20. 
    Amplification of genomic libraries is often used to generate copies of the original primary clones.
    • A. 

      TRUE

    • B. 

      FALSE