Quiz Online 5 Chapter 6 Cloning Strategies

20 Questions | By Faunsoed | Last updated: Jan 2, 2013

Settings

or Create Online Exam

- +

Quiz Online 5 Chapter 6 Cloning Strategies

Gardens are not made by sitting in the shadeTo enlarge, please click FULL SCREEN

More Online Exam Quizzes

Slu Cultural Anthropology Online Quiz 7

Animal Farm Chaptes 5-7 Quiz

Quiz Online 6 Chapter 7 The Polymerase Chain Reaction

Featured Quizzes

What Will I Look Like When I Get Older?

Will The Real Slim Shady Please Take The Quiz?

Which Female Celebrity Do You Look Like?

Related Topics

Questions and Answers

1.

The term ‘clone’ comes from the colonies of identical host cells produced during amplification of the cloned fragments.
- A.
  
  TRUE
- B.
  
  FALSE
2.

Gene cloning is sometimes referred to as ‘molecular cloning’ to distinguish the process from the cloning of whole organisms.
- A.
  
  TRUE
- B.
  
  FALSE
3.

Possible methods available for cloning procedure are the generation of DNA fragments, joining to a vector, and introducing the recombinant DNA into a host cell.
- A.
  
  TRUE
- B.
  
  FALSE
4.

A short oligo(dT) primer is annealed to the poly(A) tail on the mRNA, which provides the 3’-OH group for reverse transcriptase to begin copying the mRNA.

Discuss
- A.
  
  TRUE
- B.
  
  FALSE
5.

Linkers are synthesised chemically and can be added to cDNA by blunt-end ligation.
- A.
  
  TRUE
- B.
  
  FALSE
6.
- A.
  
  An oligo(dT) primer
- B.
  
  Complementary DNA
- C.
  
  Reverse transcriptase
- D.
  
  Flush-ended molecule
- E.
  
  The 3-OH group
7.
- A.
  
  An oligo(dT) primer
- B.
  
  Complementary DNA
- C.
  
  Reverse transcriptase
- D.
  
  Flush-ended molecule
- E.
  
  The 3-OH group
8.
- A.
  
  Complementary DNA
- B.
  
  An oligo(dT) primer
- C.
  
  Reverse transcriptase
- D.
  
  Flush-ended molecule
- E.
  
  The 3-OH group
9.
- A.
  
  The 3-OH group
- B.
  
  Complementary DNA
- C.
  
  Flush-ended molecule
- D.
  
  An oligo(dT) primer
- E.
  
  Reverse transcriptase
10.
- A.
  
  The 3-OH group
- B.
  
  Complementary DNA
- C.
  
  Reverse transcriptase
- D.
  
  An oligo(dT) primer
- E.
  
  Flush-ended molecule
11.

A colony of identical organisms; often used to describe a cell carrying a recombinant DNA fragment.
- A.
  
  Genomic library
- B.
  
  Homopolymer tailing
- C.
  
  Adaptor
- D.
  
  Linker
- E.
  
  Clone
12.

The attachment of a sequence of identical nucleotides (e.g. AAAAA) to the end of a nucleic acid molecule, usually referring to the synthesis of single-stranded homopolymer extensions on the ends of a double-stranded DNA molecule.
- A.
  
  Genomic library
- B.
  
  Homopolymer tailing
- C.
  
  Adaptor
- D.
  
  Linker
- E.
  
  Clone
13.

A collection of clones prepared from the mRNA of a given cell or tissue type, representing the genetic information expressed by such cells.
- A.
  
  Concatemer
- B.
  
  CDNA
- C.
  
  CDNA library
- D.
  
  Genomic library
14.

A DNA molecule composed of a number of individual pieces joined together via cohesive ends.
- A.
  
  Concatemer
- B.
  
  CDNA
- C.
  
  CDNA library
- D.
  
  Genomic library
15.

A collection of clones that together represent the entire genome of an organism.
- A.
  
  Concatemer
- B.
  
  CDNA
- C.
  
  CDNA library
- D.
  
  Genomic library
16.

Artificial chromosomes such as YACs or BACs can be used to clone large fragments of DNA (in excess of several hundred kb).
- A.
  
  TRUE
- B.
  
  FALSE
17.

Regulatory control of the expression of cloned genes can be achieved by selecting appropriate vector/host systems.
- A.
  
  TRUE
- B.
  
  FALSE
18.

The use of a suitable promoter is a key element in achieving the expression of a gene by means of a cloned cDNA sequence.
- A.
  
  TRUE
- B.
  
  FALSE
19.

Cloned cDNA can be used to express genes by enabling the synthesis of the protein in a suitable host system.
- A.
  
  TRUE
- B.
  
  FALSE
20.

Amplification of genomic libraries is often used to generate copies of the original primary clones.
- A.
  
  TRUE
- B.
  
  FALSE

Back to top