Quiz Online 5 Chapter 6 Cloning Strategies

20 Questions | By Faunsoed | Last updated: Jan 2, 2013

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Quiz Online 5 Chapter 6 Cloning Strategies

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Questions and Answers

1.

The term ‘clone’ comes from the colonies of identical host cells produced during amplification of the cloned fragments.
- A.
  
  TRUE
- B.
  
  FALSE
2.

Gene cloning is sometimes referred to as ‘molecular cloning’ to distinguish the process from the cloning of whole organisms.
- A.
  
  TRUE
- B.
  
  FALSE
3.

Possible methods available for cloning procedure are the generation of DNA fragments, joining to a vector, and introducing the recombinant DNA into a host cell.
- A.
  
  TRUE
- B.
  
  FALSE
4.

A short oligo(dT) primer is annealed to the poly(A) tail on the mRNA, which provides the 3’-OH group for reverse transcriptase to begin copying the mRNA.

Discuss
- A.
  
  TRUE
- B.
  
  FALSE
5.

Linkers are synthesised chemically and can be added to cDNA by blunt-end ligation.
- A.
  
  TRUE
- B.
  
  FALSE
6.
- A.
  
  An oligo(dT) primer
- B.
  
  Complementary DNA
- C.
  
  Reverse transcriptase
- D.
  
  Flush-ended molecule
- E.
  
  The 3-OH group
7.
- A.
  
  An oligo(dT) primer
- B.
  
  Complementary DNA
- C.
  
  Reverse transcriptase
- D.
  
  Flush-ended molecule
- E.
  
  The 3-OH group
8.
- A.
  
  Complementary DNA
- B.
  
  An oligo(dT) primer
- C.
  
  Reverse transcriptase
- D.
  
  Flush-ended molecule
- E.
  
  The 3-OH group
9.
- A.
  
  The 3-OH group
- B.
  
  Complementary DNA
- C.
  
  Flush-ended molecule
- D.
  
  An oligo(dT) primer
- E.
  
  Reverse transcriptase
10.
- A.
  
  The 3-OH group
- B.
  
  Complementary DNA
- C.
  
  Reverse transcriptase
- D.
  
  An oligo(dT) primer
- E.
  
  Flush-ended molecule
11.

A colony of identical organisms; often used to describe a cell carrying a recombinant DNA fragment.
- A.
  
  Genomic library
- B.
  
  Homopolymer tailing
- C.
  
  Adaptor
- D.
  
  Linker
- E.
  
  Clone
12.

The attachment of a sequence of identical nucleotides (e.g. AAAAA) to the end of a nucleic acid molecule, usually referring to the synthesis of single-stranded homopolymer extensions on the ends of a double-stranded DNA molecule.
- A.
  
  Genomic library
- B.
  
  Homopolymer tailing
- C.
  
  Adaptor
- D.
  
  Linker
- E.
  
  Clone
13.

A collection of clones prepared from the mRNA of a given cell or tissue type, representing the genetic information expressed by such cells.
- A.
  
  Concatemer
- B.
  
  CDNA
- C.
  
  CDNA library
- D.
  
  Genomic library
14.

A DNA molecule composed of a number of individual pieces joined together via cohesive ends.
- A.
  
  Concatemer
- B.
  
  CDNA
- C.
  
  CDNA library
- D.
  
  Genomic library
15.

A collection of clones that together represent the entire genome of an organism.
- A.
  
  Concatemer
- B.
  
  CDNA
- C.
  
  CDNA library
- D.
  
  Genomic library
16.

Artificial chromosomes such as YACs or BACs can be used to clone large fragments of DNA (in excess of several hundred kb).
- A.
  
  TRUE
- B.
  
  FALSE
17.

Regulatory control of the expression of cloned genes can be achieved by selecting appropriate vector/host systems.
- A.
  
  TRUE
- B.
  
  FALSE
18.

The use of a suitable promoter is a key element in achieving the expression of a gene by means of a cloned cDNA sequence.
- A.
  
  TRUE
- B.
  
  FALSE
19.

Cloned cDNA can be used to express genes by enabling the synthesis of the protein in a suitable host system.
- A.
  
  TRUE
- B.
  
  FALSE
20.

Amplification of genomic libraries is often used to generate copies of the original primary clones.
- A.
  
  TRUE
- B.
  
  FALSE

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