Cloning Strategies Quiz: Exam!
(197).jpg "Cloning Strategies Quiz: Exam!")
- 1.The term ‘clone’ comes from the colonies of identical host cells produced during the amplification of the cloned fragments.
- A.
TRUE
- B.
FALSE
- 2.Gene cloning is sometimes referred to as ‘molecular cloning’ to distinguish the process from the cloning of whole organisms.
- A.
TRUE
- B.
FALSE
- 3.Possible methods available for cloning procedure are the generation of DNA fragments, joining to a vector, and introducing the recombinant DNA into a host cell.
- A.
TRUE
- B.
FALSE
- 4.A short oligo(dT) primer is annealed to the poly(A) tail on the mRNA, which provides the 3’-OH group for reverse transcriptase to begin copying the mRNA.
- A.
TRUE
- B.
FALSE
- 5.Linkers are synthesised chemically and can be added to cDNA by blunt-end ligation.
- A.
TRUE
- B.
FALSE
- 6.
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- A.
An oligo(dT) primer
- B.
Complementary DNA
- C.
Reverse transcriptase
- D.
Flush-ended molecule
- E.
The 3-OH group
- 7.
![- ProProfs]()
- A.
An oligo(dT) primer
- B.
Complementary DNA
- C.
Reverse transcriptase
- D.
Flush-ended molecule
- E.
The 3-OH group
- 8.
![- ProProfs]()
- A.
Complementary DNA
- B.
An oligo(dT) primer
- C.
Reverse transcriptase
- D.
Flush-ended molecule
- E.
The 3-OH group
- 9.
![- ProProfs]()
- A.
The 3-OH group
- B.
Complementary DNA
- C.
Flush-ended molecule
- D.
An oligo(dT) primer
- E.
Reverse transcriptase
- 10.
![- ProProfs]()
- A.
The 3-OH group
- B.
Complementary DNA
- C.
Reverse transcriptase
- D.
An oligo(dT) primer
- E.
Flush-ended molecule
- 11.A colony of identical organisms; often used to describe a cell carrying a recombinant DNA fragment.
- A.
Genomic library
- B.
Homopolymer tailing
- C.
Adaptor
- D.
Linker
- E.
Clone
- 12.The attachment of a sequence of identical nucleotides (e.g. AAAAA) to the end of a nucleic acid molecule, usually referring to the synthesis of single-stranded homopolymer extensions on the ends of a double-stranded DNA molecule.
- A.
Genomic library
- B.
Homopolymer tailing
- C.
Adaptor
- D.
Linker
- E.
Clone
- 13.A collection of clones prepared from the mRNA of a given cell or tissue type, representing the genetic information expressed by such cells.
- A.
Concatemer
- B.
CDNA
- C.
CDNA library
- D.
Genomic library
- 14.A DNA molecule composed of a number of individual pieces joined together via cohesive ends.
- A.
Concatemer
- B.
CDNA
- C.
CDNA library
- D.
Genomic library
- 15.A collection of clones that together represent the entire genome of an organism.
- A.
Concatemer
- B.
CDNA
- C.
CDNA library
- D.
Genomic library
- 16.Artificial chromosomes such as YACs or BACs can be used to clone large fragments of DNA (in excess of several hundred kb).
- A.
TRUE
- B.
FALSE
- 17.Regulatory control of the expression of cloned genes can be achieved by selecting appropriate vector/host systems.
- A.
TRUE
- B.
FALSE
- 18.The use of a suitable promoter is a key element in achieving the expression of a gene by means of a cloned cDNA sequence.
- A.
TRUE
- B.
FALSE
- 19.Cloned cDNA can be used to express genes by enabling the synthesis of the protein in a suitable host system.
- A.
TRUE
- B.
FALSE
- 20.Amplification of genomic libraries is often used to generate copies of the original primary clones.
- A.
TRUE
- B.
FALSE
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