Genetics - 20 Difficult Questions

By Kar
Kar, Assistant Professor (Biochemistry)
Karthikeyan Pethusamy is an assistant professor in the Department of Biochemistry at the All India Institute of Medical Sciences in New Delhi
Quizzes Created: 33 | Total Attempts: 43,655
, Assistant Professor (Biochemistry)
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Questions: 20 | Attempts: 2,272

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Genetics - 20 Difficult Questions - Quiz

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Questions and Answers
  • 1. 

    Klenow fragment lacks 

    • A.

      5'→3' polymerase

    • B.

      3'→5' exonuclease

    • C.

      5'→3' exonuclease activity

    • D.

      None

    Correct Answer
    C. 5'→3' exonuclease activity
    Explanation
    Klenow Fragment is a fragment of DNA polymerase I. It was used in PCR

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  • 2. 

    Similarity between DNA polymerase and Glycogen synthase

    • A.

      Both need a primer

    • B.

      Both enzymes are processive in adding substrates

    • C.

      Either of the above

    • D.

      None

    Correct Answer
    C. Either of the above
    Explanation
    processiivity is the ability to continue of continous addition without dissociating from its substrate.

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  • 3. 

    Basic protein that binds to DNA in spermatid

    • A.

      Histone

    • B.

      Protamine

    • C.

      Polyamine

    • D.

      None

    Correct Answer
    B. Protamine
    Explanation
    protamines are small arginine-rich proteins helping in sperm head stabilization in the sperm head

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  • 4. 

    Signal recognition particle contains

    • A.

      28S RNA

    • B.

      60S RNA

    • C.

      40S RNA

    • D.

      7S RNA

    Correct Answer
    D. 7S RNA
    Explanation
    SRP is a ribonucleoprotein that binds to N-terminal signal sequence of the proteins to be associated with ER.

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  • 5. 

    Possibly more than 90% of genome is transcribed. 

    • A.

      True

    • B.

      False

    Correct Answer
    A. True
    Explanation
    Not all of them are translated. Many of them are long non coding RNA, rRNA, snRNA, miRNA, SnoRNA, SiRNA.

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  • 6. 

    Carboxy terminal domain (CTD) of RNA polymerase is phosphorylated by 

    • A.

      TFIID

    • B.

      TFIIA

    • C.

      TFIIB

    • D.

      TFIIH

    Correct Answer
    D. TFIIH
    Explanation
    TFIIH has helicase and kinase activity. CTD phosphorylation is important for the elongation to begin.

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  • 7. 

    Special transcription factors bind to

    • A.

      TATA box

    • B.

      Inr element

    • C.

      DPE

    • D.

      CAAT box

    Correct Answer
    D. CAAT box
    Explanation
    others are cis-elements for binding of trans acting basal transcription factors.

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  • 8. 

    Which is NOT a DNA binding domain ?(Don't worry, No marks for this question)

    • A.

      Zinc finger

    • B.

      Helix-turn-Helix

    • C.

      Helix-loop-helix

    • D.

      Basic domains

    Correct Answer
    C. Helix-loop-helix
    Explanation
    Helix loop helix won't bind to DNA. It is a dimerization domain like leucine zipper. (Very important)
    (ps I'll try to make a video for this topic)

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  • 9. 

    Which is a template independent DNA polymerase ?

    • A.

      Poly A polymerase

    • B.

      CCA adding enzyme

    • C.

      Terminal deoxynucleotidyl transferase

    • D.

      None

    Correct Answer
    C. Terminal deoxynucleotidyl transferase
    Explanation
    Usually DNA & Polymerases need a template.
    But certain polymerases are unique and can add nucleotides without a template.

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  • 10. 

    Sequence of an intron always starts and ends with(Don't worry, No marks for this question)

    • A.

      GU,AG

    • B.

      AG,GU

    • C.

      GA,GU

    • D.

      UG,AG

    Correct Answer
    A. GU,AG
    Explanation
    The correct answer is GU,AG. This is because the sequence of an intron always starts with GU and ends with AG. This is a consensus sequence that is recognized by the spliceosome, which is responsible for removing introns from pre-mRNA during the process of splicing. The GU at the 5' end of the intron is recognized by the U1 snRNP, while the AG at the 3' end is recognized by the U2AF protein. These sequences are essential for proper splicing and the removal of introns from the mRNA transcript.

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  • 11. 

    RNA splicing requires ATP hydrolysis.

    • A.

      True

    • B.

      False

    Correct Answer
    B. False
    Explanation
    As the number of phosphate-ester bond is unchanged, no energy is consumed.

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  • 12. 

    Instead of poly A tail histone pre-mRNA has stem-loop structure.

    • A.

      True

    • B.

      False

    Correct Answer
    A. True
    Explanation
    Fact !

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  • 13. 

    5S RNA is transcribed by

    • A.

      RNA polymerase I

    • B.

      RNA polymerase II

    • C.

      RNA polymerase III

    • D.

      All of the above

    Correct Answer
    C. RNA polymerase III
    Explanation
    All rRNAs are transcribed by RNAP I except 5sRNA.

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  • 14. 

    Self-splicing is mediated by (No marks for this question!!!)

    • A.

      Adenosine

    • B.

      Guanosine

    • C.

      Cytosine

    • D.

      None

    Correct Answer
    B. Guanosine
    Explanation
    Splicesome mediated splicing requires OH group of adenosine.

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  • 15. 

    Promoters of eukaryotic tRNA lie within the gene itself. 

    • A.

      True

    • B.

      False

    Correct Answer
    A. True
    Explanation
    Intergenic promoters.

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  • 16. 

    Which amino acid(s) is/are coded by a single codon

    • A.

      Tryptophan

    • B.

      Methionine

    • C.

      Both

    • D.

      None

    Correct Answer
    C. Both
    Explanation
    AUG for methionine and UGG for tryptophan

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  • 17. 

    ApoB48 & apoB100 production is due to

    • A.

      Alternative splicing

    • B.

      MRNA editing

    • C.

      Alternative polyadenylation

    • D.

      MRNA silencing

    Correct Answer
    B. MRNA editing
    Explanation
    CAA, 2152 nd nucleotide of 4536 long apoB100 mRNA is acted upon by cytosine deaminase to produce UAA, stop codon resulting in production of apoB48.

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  • 18. 

    Mirtrons are miRNAs located in introns

    • A.

      True

    • B.

      False

    Correct Answer
    A. True
    Explanation
    Fact !

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  • 19. 

    All ribonucleases are ribozymes

    • A.

      True

    • B.

      False

    Correct Answer
    B. False
    Explanation
    RNAse P is the only ribozyme among ribonucleases.

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  • 20. 

    During translation, initiator tRNA binds to 

    • A.

      A site

    • B.

      P site

    • C.

      E site

    • D.

      None

    Correct Answer
    B. P site
    Explanation
    initiator tRNA is the only tRNA that comes and binds to P site. All others will bind to A site.

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Kar |Assistant Professor (Biochemistry) |
Karthikeyan Pethusamy is an assistant professor in the Department of Biochemistry at the All India Institute of Medical Sciences in New Delhi

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