Quiz Genomic Libraries & Cdna Libraries

10 Questions | Total Attempts: 1730

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Quiz Genomic Libraries & Cdna Libraries

Presenter: GENOMIC LIBRARIES & cDNA LIBRARIES Kelompok 9 & 10 Penyanggah: GENOMIC LIBRARIES & cDNA LIBRARIES Kelompok 11 & 12


Questions and Answers
  • 1. 
    Gene libraries made from genomic DNA are called genomic libraries and those made from complementary DNA are known as cDNA libraries.
    • A. 

      TRUE

    • B. 

      FALSE

  • 2. 
    Engineered plasmids are used extensively as vectors for cloning.
    • A. 

      TRUE

    • B. 

      FALSE

  • 3. 
    This vector can be used to construct genomic libraries.
    • A. 

      Plasmid

    • B. 

      Phage

    • C. 

      Cosmid

    • D. 

      BAC

    • E. 

      All of the above

  • 4. 
    MRNA can be readily isolated from lysed eukaryotic cells by adding magnetic beads which have _______ covalently attached.
    • A. 

      Oligo(dA)

    • B. 

      Oligo(dT)

    • C. 

      Oligo(dG)

    • D. 

      Oligo(dC)

    • E. 

      All of the above

  • 5. 
    This enzyme is used to  dephosphorylate the vector.
    • A. 

      Terminal transferase

    • B. 

      Alkaline phosphatase

    • C. 

      Klenow enzyme

    • D. 

      Reverse transcriptase

    • E. 

      DNA ligase

  • 6. 
    Generally, cDNA libraries are made using prokaryotic mRNA.
    • A. 

      TRUE

    • B. 

      FALSE

  • 7. 
    Usually, special nucleic acid  linkers are added to create sticky ends for cloning.
    • A. 

      TRUE

    • B. 

      FALSE

  • 8. 
    Making cDNA libraries from eukaryotic mRNA is very useful because the cDNAs have no gene sequences.
    • A. 

      TRUE

    • B. 

      FALSE

  • 9. 
    Since cDNAs are derived from the mRNA, cDNAs represent the transcribed parts of the genome.
    • A. 

      TRUE

    • B. 

      FALSE

  • 10. 
    In eukaryotes, most mRNAs are polyadenylated  and this 3’-tail of about 200 thymine residues provides a useful method for isolating eukaryotic mRNA.
    • A. 

      TRUE

    • B. 

      FALSE

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