Quiz Genomic Libraries & Cdna Libraries
10 Questions
| Total Attempts: 1730
Presenter: GENOMIC LIBRARIES & cDNA LIBRARIES Kelompok 9 & 10 Penyanggah: GENOMIC LIBRARIES & cDNA LIBRARIES Kelompok 11 & 12
Questions and Answers
- 1.Gene libraries made from genomic DNA are called genomic libraries and those made from complementary DNA are known as cDNA libraries.
- A.
TRUE
- B.
FALSE
- 2.Engineered plasmids are used extensively as vectors for cloning.
- A.
TRUE
- B.
FALSE
- 3.This vector can be used to construct genomic libraries.
- A.
Plasmid
- B.
Phage
- C.
Cosmid
- D.
BAC
- E.
All of the above
- 4.MRNA can be readily isolated from lysed eukaryotic cells by adding magnetic beads which have _______ covalently attached.
- A.
Oligo(dA)
- B.
Oligo(dT)
- C.
Oligo(dG)
- D.
Oligo(dC)
- E.
All of the above
- 5.This enzyme is used to dephosphorylate the vector.
- A.
Terminal transferase
- B.
Alkaline phosphatase
- C.
Klenow enzyme
- D.
Reverse transcriptase
- E.
DNA ligase
- 6.Generally, cDNA libraries are made using prokaryotic mRNA.
- A.
TRUE
- B.
FALSE
- 7.Usually, special nucleic acid linkers are added to create sticky ends for cloning.
- A.
TRUE
- B.
FALSE
- 8.Making cDNA libraries from eukaryotic mRNA is very useful because the cDNAs have no gene sequences.
- A.
TRUE
- B.
FALSE
- 9.Since cDNAs are derived from the mRNA, cDNAs represent the transcribed parts of the genome.
- A.
TRUE
- B.
FALSE
- 10.In eukaryotes, most mRNAs are polyadenylated and this 3’-tail of about 200 thymine residues provides a useful method for isolating eukaryotic mRNA.
- A.
TRUE
- B.
FALSE
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