Pre-uts Quiz In Molecular Biology 2009

23 Questions | By Fabiounsoed | Last updated: Dec 31, 2012 | Total Attempts: 275

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Insstruction for question 1 - 15:Each question below contains four suggested responses. Please choose by clicking the one best response to each questionIntruction for question 16-25: Powerful molecular biology techniques now allow us to amplify, analyze, and compare genes. Review these methods by matching each phrase on the left with a term on the right. Terms may be used MORE THAN ONCE or NOT AT ALL.

Questions and Answers

1.

Which of the following statement is CORRECT concerning a genome?
- A.
  
  The totality of genetic information belonging to a cell or an organism
- B.
  
  The science of studying the DNA sequences and properties of entire genomes.
- C.
  
  The genome present in the mitochondria of a eukaryotic cell.
- D.
  
  The DNA molecules present in the nucleus of a eukaryotic cell.
2.

Which of the following is NOT a component of nucleotide of DNA?
- A.
  
  2′-deoxyribose
- B.
  
  A nitrogenous base
- C.
  
  A phosphate group
- D.
  
  Ribose
3.

Which of the following is an accurate statement concerning the differences between DNA and RNA?
- A.
  
  RNA is usually double-stranded, but DNA is usually single-stranded.
- B.
  
  RNA has the sugar deoxyribose, but DNA has the sugar ribose.
- C.
  
  RNA contains three different nucleotides, but DNA contains four different nucleotides.
- D.
  
  RNA lacks the base thymine (which is found in DNA) and has uracil instead.
4.

RNA polynucleotides contain 3′–5′ phosphodiester bonds, but these phosphodiester bonds are less stable than those in a DNA polynucleotide. This phenomenon is caused by the indirect effect of the hydroxyl group at _______
- A.
  
  The 3′-position of the sugar
- B.
  
  The 2′-position of the sugar
- C.
  
  The 1′-position of the sugar
- D.
  
  The 5′-position of the sugar
5.

Who carried out the base ratio studies of DNA?
- A.
  
  Erwin Chargaff
- B.
  
  Colin MacLeod and Maclyn McCarty
- C.
  
  Alfred Hershey and Martha Chase
- D.
  
  Watson and Crick
6.

Which of the following is INCORRECT concerning the genome size?
- A.
  
  Fungi having the smallest genomes
- B.
  
  Vertebrates and flowering plants having the largest genomes
- C.
  
  Escherichia coli K12 genome is smaller than Drosophila melanogaster genome.
- D.
  
  Saccharomyces cerevisiae genome is smaller than E. coli K12 genome
7.

Which of the following is INCORRECT concerning the gene categories in a eukaryotic genome?
- A.
  
  The genes can be classified according to their function
- B.
  
  The genes can be categorized according to the protein domains that they specify.
- C.
  
  Th protein structure classification can be applied to genes whose functions are not known.
- D.
  
  Th gene function classification can encompass a larger proportion of the set of genes in a genome.
8.

Which of the following type of repetitive DNA have arisen by transposition?
- A.
  
  Tandemly repeated DNA
- B.
  
  Interspersed repeats
- C.
  
  Minisatellite
- D.
  
  Microsatellites
9.

The mode of transposition that involves an RNA intermediate, is known as ___________
- A.
  
  Retrotransposition
- B.
  
  Replicative transposition
- C.
  
  Conservative transposition
- D.
  
  Replication slippage
10.

What is the initial product of genome expression?
- A.
  
  Transcriptome
- B.
  
  Proteome
- C.
  
  Genome
- D.
  
  Metagenome
11.

All of the following statements about the genetic content of organelle genomes are true, EXCEPT
- A.
  
  Organelle genomes are much smaller than their nuclear genomes
- B.
  
  All mitochondrial genomes contain genes for the non-coding rRNAs
- C.
  
  Organelle genomes specify all of the proteins found in the organelle
- D.
  
  Most chloroplast genomes possess gen for code rRNAs and tRNAs
12.

Which of the following is ‘satellite' DNA that formed by clusters up to 20 kb in length, with repeat units up to 25 bp?
- A.
  
  Minisatellite
- B.
  
  Microsatellites
- C.
  
  Macrosatellites
- D.
  
  Nanosatellites
13.

Which of the following is NOT an correct reason concerning the importance of the Human Genome Project?
- A.
  
  To make the human gene catalog
- B.
  
  To understanding how the information contained in the genome is utilized by the cell.
- C.
  
  To understanding the organization of the intergenic DNA in human genomes
- D.
  
  To deduce the double helix structure
14.

Powerful molecular biology techniques now allow us to amplify, analyze, and compare genes. Review these methods by matching each phrase on the left with a term on the right. Terms may be used MORE THAN ONCE or NOT AT ALL. 16 This plasmid carries genes coding for enzymes that enable the host bacterium to withstand the growth-inhibitory effects of two antibiotics: ampicillin and tetracycline A. Alkaline phosphatase B. DNA polymerases C. pBR322 D. PCR E. Chemical degradation sequencing F. Primer G. Cloning vector H. Sequencing I. Ligases J. Restriction endonuclease K. Sticky or cohesive ends L. Nucleases M. Chain termination method N. Selectable markers O.Template 17 An enzyme that cuts DNA molecules at a limited number of specific nucleotide sequences. 18 Enzymes that join DNA molecules together by synthesizing phosphodiester bonds between nucleotides 19 A technique that results in exponential amplification of a selected region of a DNA molecule 20 A oligonucleotides that is used in a polymerization reaction to produce a complementary DNA or RNA strand 21 The linear ends of a dsDNA molecule that have some single-stranded bases on their ends 22 A gene carried by a vector and conferring a recognizable characteristic on a cell containing the vector or a recombinant DNA molecule derived from the vector. 23 A DNA molecule that is able to replicate inside a host cell and therefore can be used to clone other fragments of DNA 24 The methods for sequencing DNA that was developed by Fred Sanger 25 A DNA sequencing method that involves enzymatic synthesis of polynucleotide chains that terminate at specific nucleotide positions.
15.

Powerful molecular biology techniques now allow us to amplify, analyze, and compare genes. Review these methods by matching each phrase on the left with a term on the right. Terms may be used MORE THAN ONCE or NOT AT ALL. 16 This plasmid carries genes coding for enzymes that enable the host bacterium to withstand the growth-inhibitory effects of two antibiotics: ampicillin and tetracycline A. Alkaline phosphatase B. DNA polymerases C. pBR322 D. PCR E. Chemical degradation sequencing F. Primer G. Cloning vector H. Sequencing I. Ligases J. Restriction endonuclease K. Sticky or cohesive ends L. Nucleases M. Chain termination method N. Selectable markers O.Template 17 An enzyme that cuts DNA molecules at a limited number of specific nucleotide sequences. 18 Enzymes that join DNA molecules together by synthesizing phosphodiester bonds between nucleotides 19 A technique that results in exponential amplification of a selected region of a DNA molecule 20 A oligonucleotides that is used in a polymerization reaction to produce a complementary DNA or RNA strand 21 The linear ends of a dsDNA molecule that have some single-stranded bases on their ends 22 A gene carried by a vector and conferring a recognizable characteristic on a cell containing the vector or a recombinant DNA molecule derived from the vector. 23 A DNA molecule that is able to replicate inside a host cell and therefore can be used to clone other fragments of DNA 24 The methods for sequencing DNA that was developed by Fred Sanger 25 A DNA sequencing method that involves enzymatic synthesis of polynucleotide chains that terminate at specific nucleotide positions.
16.

Powerful molecular biology techniques now allow us to amplify, analyze, and compare genes. Review these methods by matching each phrase on the left with a term on the right. Terms may be used MORE THAN ONCE or NOT AT ALL. 16 This plasmid carries genes coding for enzymes that enable the host bacterium to withstand the growth-inhibitory effects of two antibiotics: ampicillin and tetracycline A. Alkaline phosphatase B. DNA polymerases C. pBR322 D. PCR E. Chemical degradation sequencing F. Primer G. Cloning vector H. Sequencing I. Ligases J. Restriction endonuclease K. Sticky or cohesive ends L. Nucleases M. Chain termination method N. Selectable markers O.Template 17 An enzyme that cuts DNA molecules at a limited number of specific nucleotide sequences. 18 Enzymes that join DNA molecules together by synthesizing phosphodiester bonds between nucleotides 19 A technique that results in exponential amplification of a selected region of a DNA molecule 20 A oligonucleotides that is used in a polymerization reaction to produce a complementary DNA or RNA strand 21 The linear ends of a dsDNA molecule that have some single-stranded bases on their ends 22 A gene carried by a vector and conferring a recognizable characteristic on a cell containing the vector or a recombinant DNA molecule derived from the vector. 23 A DNA molecule that is able to replicate inside a host cell and therefore can be used to clone other fragments of DNA 24 The methods for sequencing DNA that was developed by Fred Sanger 25 A DNA sequencing method that involves enzymatic synthesis of polynucleotide chains that terminate at specific nucleotide positions.
17.

Powerful molecular biology techniques now allow us to amplify, analyze, and compare genes. Review these methods by matching each phrase on the left with a term on the right. Terms may be used MORE THAN ONCE or NOT AT ALL. 16 This plasmid carries genes coding for enzymes that enable the host bacterium to withstand the growth-inhibitory effects of two antibiotics: ampicillin and tetracycline A. Alkaline phosphatase B. DNA polymerases C. pBR322 D. PCR E. Chemical degradation sequencing F. Primer G. Cloning vector H. Sequencing I. Ligases J. Restriction endonuclease K. Sticky or cohesive ends L. Nucleases M. Chain termination method N. Selectable markers O.Template 17 An enzyme that cuts DNA molecules at a limited number of specific nucleotide sequences. 18 Enzymes that join DNA molecules together by synthesizing phosphodiester bonds between nucleotides 19 A technique that results in exponential amplification of a selected region of a DNA molecule 20 A oligonucleotides that is used in a polymerization reaction to produce a complementary DNA or RNA strand 21 The linear ends of a dsDNA molecule that have some single-stranded bases on their ends 22 A gene carried by a vector and conferring a recognizable characteristic on a cell containing the vector or a recombinant DNA molecule derived from the vector. 23 A DNA molecule that is able to replicate inside a host cell and therefore can be used to clone other fragments of DNA 24 The methods for sequencing DNA that was developed by Fred Sanger 25 A DNA sequencing method that involves enzymatic synthesis of polynucleotide chains that terminate at specific nucleotide positions.
18.

Powerful molecular biology techniques now allow us to amplify, analyze, and compare genes. Review these methods by matching each phrase on the left with a term on the right. Terms may be used MORE THAN ONCE or NOT AT ALL. 16 This plasmid carries genes coding for enzymes that enable the host bacterium to withstand the growth-inhibitory effects of two antibiotics: ampicillin and tetracycline A. Alkaline phosphatase B. DNA polymerases C. pBR322 D. PCR E. Chemical degradation sequencing F. Primer G. Cloning vector H. Sequencing I. Ligases J. Restriction endonuclease K. Sticky or cohesive ends L. Nucleases M. Chain termination method N. Selectable markers O.Template 17 An enzyme that cuts DNA molecules at a limited number of specific nucleotide sequences. 18 Enzymes that join DNA molecules together by synthesizing phosphodiester bonds between nucleotides 19 A technique that results in exponential amplification of a selected region of a DNA molecule 20 A oligonucleotides that is used in a polymerization reaction to produce a complementary DNA or RNA strand 21 The linear ends of a dsDNA molecule that have some single-stranded bases on their ends 22 A gene carried by a vector and conferring a recognizable characteristic on a cell containing the vector or a recombinant DNA molecule derived from the vector. 23 A DNA molecule that is able to replicate inside a host cell and therefore can be used to clone other fragments of DNA 24 The methods for sequencing DNA that was developed by Fred Sanger 25 A DNA sequencing method that involves enzymatic synthesis of polynucleotide chains that terminate at specific nucleotide positions.
19.

Powerful molecular biology techniques now allow us to amplify, analyze, and compare genes. Review these methods by matching each phrase on the left with a term on the right. Terms may be used MORE THAN ONCE or NOT AT ALL. 16 This plasmid carries genes coding for enzymes that enable the host bacterium to withstand the growth-inhibitory effects of two antibiotics: ampicillin and tetracycline A. Alkaline phosphatase B. DNA polymerases C. pBR322 D. PCR E. Chemical degradation sequencing F. Primer G. Cloning vector H. Sequencing I. Ligases J. Restriction endonuclease K. Sticky or cohesive ends L. Nucleases M. Chain termination method N. Selectable markers O.Template 17 An enzyme that cuts DNA molecules at a limited number of specific nucleotide sequences. 18 Enzymes that join DNA molecules together by synthesizing phosphodiester bonds between nucleotides 19 A technique that results in exponential amplification of a selected region of a DNA molecule 20 A oligonucleotides that is used in a polymerization reaction to produce a complementary DNA or RNA strand 21 The linear ends of a dsDNA molecule that have some single-stranded bases on their ends 22 A gene carried by a vector and conferring a recognizable characteristic on a cell containing the vector or a recombinant DNA molecule derived from the vector. 23 A DNA molecule that is able to replicate inside a host cell and therefore can be used to clone other fragments of DNA 24 The methods for sequencing DNA that was developed by Fred Sanger 25 A DNA sequencing method that involves enzymatic synthesis of polynucleotide chains that terminate at specific nucleotide positions.
20.

Powerful molecular biology techniques now allow us to amplify, analyze, and compare genes. Review these methods by matching each phrase on the left with a term on the right. Terms may be used MORE THAN ONCE or NOT AT ALL. 16 This plasmid carries genes coding for enzymes that enable the host bacterium to withstand the growth-inhibitory effects of two antibiotics: ampicillin and tetracycline A. Alkaline phosphatase B. DNA polymerases C. pBR322 D. PCR E. Chemical degradation sequencing F. Primer G. Cloning vector H. Sequencing I. Ligases J. Restriction endonuclease K. Sticky or cohesive ends L. Nucleases M. Chain termination method N. Selectable markers O.Template 17
21.

Powerful molecular biology techniques now allow us to amplify, analyze, and compare genes. Review these methods by matching each phrase on the left with a term on the right. Terms may be used MORE THAN ONCE or NOT AT ALL. 16 This plasmid carries genes coding for enzymes that enable the host bacterium to withstand the growth-inhibitory effects of two antibiotics: ampicillin and tetracycline A. Alkaline phosphatase B. DNA polymerases C. pBR322 D. PCR E. Chemical degradation sequencing F. Primer G. Cloning vector H. Sequencing I. Ligases J. Restriction endonuclease K. Sticky or cohesive ends L. Nucleases M. Chain termination method N. Selectable markers O.Template 17 An enzyme that cuts DNA molecules at a limited number of specific nucleotide sequences. 18 Enzymes that join DNA molecules together by synthesizing phosphodiester bonds between nucleotides 19 A technique that results in exponential amplification of a selected region of a DNA molecule 20 A oligonucleotides that is used in a polymerization reaction to produce a complementary DNA or RNA strand 21 The linear ends of a dsDNA molecule that have some single-stranded bases on their ends 22 A gene carried by a vector and conferring a recognizable characteristic on a cell containing the vector or a recombinant DNA molecule derived from the vector. 23 A DNA molecule that is able to replicate inside a host cell and therefore can be used to clone other fragments of DNA 24 The methods for sequencing DNA that was developed by Fred Sanger 25 A DNA sequencing method that involves enzymatic synthesis of polynucleotide chains that terminate at specific nucleotide positions.
22.

Powerful molecular biology techniques now allow us to amplify, analyze, and compare genes. Review these methods by matching each phrase on the left with a term on the right. Terms may be used MORE THAN ONCE or NOT AT ALL. 16 This plasmid carries genes coding for enzymes that enable the host bacterium to withstand the growth-inhibitory effects of two antibiotics: ampicillin and tetracycline A. Alkaline phosphatase B. DNA polymerases C. pBR322 D. PCR E. Chemical degradation sequencing F. Primer G. Cloning vector H. Sequencing I. Ligases J. Restriction endonuclease K. Sticky or cohesive ends L. Nucleases M. Chain termination method N. Selectable markers O.Template 17 An enzyme that cuts DNA molecules at a limited number of specific nucleotide sequences. 18 Enzymes that join DNA molecules together by synthesizing phosphodiester bonds between nucleotides 19 A technique that results in exponential amplification of a selected region of a DNA molecule 20 A oligonucleotides that is used in a polymerization reaction to produce a complementary DNA or RNA strand 21 The linear ends of a dsDNA molecule that have some single-stranded bases on their ends 22 A gene carried by a vector and conferring a recognizable characteristic on a cell containing the vector or a recombinant DNA molecule derived from the vector. 23 A DNA molecule that is able to replicate inside a host cell and therefore can be used to clone other fragments of DNA 24 The methods for sequencing DNA that was developed by Fred Sanger 25 A DNA sequencing method that involves enzymatic synthesis of polynucleotide chains that terminate at specific nucleotide positions.
23.

Powerful molecular biology techniques now allow us to amplify, analyze, and compare genes. Review these methods by matching each phrase on the left with a term on the right. Terms may be used MORE THAN ONCE or NOT AT ALL. 16 This plasmid carries genes coding for enzymes that enable the host bacterium to withstand the growth-inhibitory effects of two antibiotics: ampicillin and tetracycline A. Alkaline phosphatase B. DNA polymerases C. pBR322 D. PCR E. Chemical degradation sequencing F. Primer G. Cloning vector H. Sequencing I. Ligases J. Restriction endonuclease K. Sticky or cohesive ends L. Nucleases M. Chain termination method N. Selectable markers O.Template 17 An enzyme that cuts DNA molecules at a limited number of specific nucleotide sequences. 18 Enzymes that join DNA molecules together by synthesizing phosphodiester bonds between nucleotides 19 A technique that results in exponential amplification of a selected region of a DNA molecule 20 A oligonucleotides that is used in a polymerization reaction to produce a complementary DNA or RNA strand 21 The linear ends of a dsDNA molecule that have some single-stranded bases on their ends 22 A gene carried by a vector and conferring a recognizable characteristic on a cell containing the vector or a recombinant DNA molecule derived from the vector. 23 A DNA molecule that is able to replicate inside a host cell and therefore can be used to clone other fragments of DNA 24 The methods for sequencing DNA that was developed by Fred Sanger 25 A DNA sequencing method that involves enzymatic synthesis of polynucleotide chains that terminate at specific nucleotide positions.