Molecular Biology Practice Test

75 Questions | Total Attempts: 684

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Molecular Biology Practice Test - Quiz

Do you wish to practice molecular biology? Take this test and see how well you understand molecular biology or if you need to learn more about it. We have got a question about molecular biology that will help you recall whatever you have learned and prepare for any upcoming exams. This exam cover chapters 14,15,17, 18 and 24. It will be an easy quiz for you, as your concepts of molecular biology are already clear. All the best! Even if you miss out on some question, we are here to help you with the correct answer.


Questions and Answers
  • 1. 
    Which of the following experiments was useful in determining the existence of introns in the adenovirus genome?
    • A. 

      DNA/DNA hybridization

    • B. 

      RNA/RNA hybridization

    • C. 

      R-looping experiments

    • D. 

      RT-PCR

    • E. 

      CDNA cloning experiments

  • 2. 
    Which enzyme is used in the unwinding of DNA?
    • A. 

      Exonuclease

    • B. 

      Helicase

    • C. 

      Topoisomerase

    • D. 

      Ligase

  • 3. 
    You have isolated a new species of mRNA using an oligo dT column. You then perform an R-looping experiment to determine if the gene encoding for your mRNA contains introns. Below is a representation of the electron micrograph results. Which of the following can you conclude from your experiment? 
    • A. 

      The gene contains no introns.

    • B. 

      The gene contains one intron.

    • C. 

      The gene contains two introns.

    • D. 

      The gene contains three intron.

  • 4. 
    The function of EF-Tu is to 
    • A. 

      Properly fold proteins

    • B. 

      Ensure proper ribosome assembly

    • C. 

      Protect rRNA from the degradation of RNase

    • D. 

      Escort aminoacyl-tRNA to the ribosome

    • E. 

      Stabilize mRNA within the ribosome

  • 5. 
    You have isolated a new species of mRNA using an oligo-dT column. You then perform an R-looping experiment to determine if the gene encoding for your mRNA contains introns. Below is a representation of the electron micrograph results. Which of the following can you conclude from your experiment?
    • A. 

      The gene contains no introns

    • B. 

      The gene contains one intron

    • C. 

      The gene contains two introns

    • D. 

      The gene contains three introns

    • E. 

      The results are inconclusive

  • 6. 
    The first two bases and the last two bases in the splicing signal consensus sequence are
    • A. 

      GT-AG

    • B. 

      GU-AG

    • C. 

      CU-AG

    • D. 

      GU-AC

    • E. 

      GT-TG

  • 7. 
    Which of the following is the name for the YEAST 40s particle where mRNA splicing takes place?
    • A. 

      Ribosome

    • B. 

      Nucleolus

    • C. 

      Spliceosome

    • D. 

      HnRNA

    • E. 

      R-loop

  • 8. 
    "Snurps" or snRNPs are composed of
    • A. 

      RNA

    • B. 

      Protein

    • C. 

      DNA

    • D. 

      RNA and protein

    • E. 

      DNA and RNA

  • 9. 
    The catalytic center of the spliceosome appears to include
    • A. 

      Mg2+

    • B. 

      U2 and U6 snRNP

    • C. 

      The branch point region of the intron

    • D. 

      Mg2+ and the branch point region of the intron

    • E. 

      Mg2+, U2, and U6 snRNP, and the branch point region of the intron

  • 10. 
    Which of the following is the first snRNP to bind during the assembly stage of the spliceosome cycle?
    • A. 

      U1

    • B. 

      U2

    • C. 

      U4

    • D. 

      U5

    • E. 

      U6

  • 11. 
    Which of the following snRNP is mismatched with its function?
    • A. 

      U1: base pairs with 5' splice site of mRNA

    • B. 

      U2: base pairs with the conserved sequence at splicing branch point

    • C. 

      U4: base pairs with 3' splice site of mRNA

    • D. 

      U5: associates with the last nucleotide in one exon and the first nucleotide in the next exon.

    • E. 

      U6: base pairs with 5' end of the intron

  • 12. 
    The binding of which of the following snRNPs to spliceosome requires ATP?
    • A. 

      U1

    • B. 

      U2

    • C. 

      U4

    • D. 

      U5

    • E. 

      U6

  • 13. 
    Spliceosomal lariat is
    • A. 

      Cowboy's lasso

    • B. 

      Texan lariat

    • C. 

      Hairpin RNA

    • D. 

      Stem and loop DNA

    • E. 

      Splicing intermediate

  • 14. 
    Please put the following steps of Cap synthesis in the correct order. (1) N7 of the capping guanine is methylated (2) The terminal phosphate is removed from the pre-mRNA(3) A capping GMP is added to the pre-mRNA(4) The 2'-O-methyl group of the penultimate nucleotide is methylated
    • A. 

      1,2,3,4

    • B. 

      1,4,3,2

    • C. 

      2,4,1,3

    • D. 

      2,3,1,4

    • E. 

      4,3,2,1

  • 15. 
    Which of the following is NOT a function of the mRNA Cap?
    • A. 

      Protects the mRNA from degradation

    • B. 

      Enhances translatability of the mRNA

    • C. 

      Enhances the transport of the mRNA to the cytoplasm

    • D. 

      Enhances splicing of the mRNA

    • E. 

      Helps regulate the expression of the mRNA from the promoter

  • 16. 
    Although snRNA U1 functions in the nucleus, it receives a 5' Cap to enhance its
    • A. 

      Binding to snRNPs

    • B. 

      Export to the cytoplasm

    • C. 

      Export to the ER lumen

    • D. 

      Export out of the cell

    • E. 

      Binding to the mature mRNA

  • 17. 
    Which of the following is the mRNA polyadenylation signal motif found in mammalian cells?
    • A. 

      AAGAAA

    • B. 

      AAUAAA

    • C. 

      AAUGGG

    • D. 

      UUAUUU

    • E. 

      GGAUUU

  • 18. 
    When does the capping of the pre-mRNA occur?
    • A. 

      It is the first nucleotide added by RNA polymerase.

    • B. 

      It occurs before the mRNA reaches a chain length of 30 nt.

    • C. 

      It occurs after the poly (A) tail has been added.

    • D. 

      It occurs after splicing has occurred.

    • E. 

      It occurs after the export of the mRNA into the cytoplasm.

  • 19. 
    Which of the following is not required for mammalian cell polyadenylation of pre-mRNA?
    • A. 

      PAP

    • B. 

      CPSF

    • C. 

      PABII

    • D. 

      DNA polymerase I

    • E. 

      RNA polymerase II

  • 20. 
    Uncapped mRNA is much more stable than capped mRNA.
    • A. 

      True

    • B. 

      False

  • 21. 
    The 5' cap of mRNA has no effect on the translatability of the mRNA.
    • A. 

      True

    • B. 

      False

  • 22. 
    Poly (A) tails are added to the 5' end of the mRNA molecule.
    • A. 

      True

    • B. 

      False

  • 23. 
    The addition of poly (A) to mRNA seems to be important for its translatability.
    • A. 

      True

    • B. 

      False

  • 24. 
    RNA polymerase II transcription of a gene usually stops at the polyadenylation site.
    • A. 

      True

    • B. 

      False

  • 25. 
    Pre-mRNA must be cleaved before it is polyadenylated.
    • A. 

      True

    • B. 

      False

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