Microbiology: Chapter 9

1. Transformation was discovered by Griffeth in an experiment that eventually led to the idea that DNA is the genetic material.

True

False

2. What is the correct direction from which information flows?

RNA>Proteins>DNA

DNA>Proteins>RNA

DNA>RNA>Proteins

RNA>DNA>Proteins

3. Specialized transduction is

Any bacteriophage can be involved and any genes may be transferred

Only temperate phages may be involved and only the genes adjacent to their integration sites may be transferred.

4. What plasmid was found when conjugation was initially discovered?

D Factor

F Factor

E Factor

A Factor

5. Cells containing the F factor are referred to as what?

F

F+

F-

6. A mix with living rough strain of bacteria with dead smooth strain bacteria, injected into the mouse equals which of these?

No Problem

Minor health problems

Death

7. The smooth strains of bacteria produced a/an __________ in the mice, and rough strains did not. We know this because of the ______.

Increase in health, capsule

Increase in illness, capsule

Decrease in energy, virus

8. If the F factor becomes integrated into the bacterial chromosome, we call that an Hfr strain because when it conjugates it causes a...

Low frequency of recombination of bacterial genes

Slight frequency of recombination of bacterial genes

High frequency of recombination of bacterial genes

9. Generalized transduction is

Any bacteriophage can be involved and any genes may be transferred

Only temperate phages may be involved and only the genes adjacent to their integration sites may be transferred.

10. The transfer of genetic material from one bacterial cell (the donor) to another (the recipient) by direct contact.

Transduction

Conjugation

Transformation

11. The cell containing the F factor can change cells that are?

F-

F+

F

12. Match the word to its definition: Transcription

DNA copies itself before cell division

DNA makes a mRNA copy of itself during

Messenger RNA directs the synthesis of a protein

13. The transfer of bacterial genes from one cell to another mediated by a bacteriophage (a virus that infects bacteria).

Transduction

Conjugation

Transformation

14. Match the word to its definition: Replication

DNA copies itself before cell division

DNA makes a mRNA copy of itself during

Messenger RNA directs the synthesis of a protein

15. During conjugation a plasmid ______, and directs the formation of the pilus, from this the _____moves to a recipient cell

Copies itself, copy of it

Reaches, piece of it

Moves, metabolite

16. Was Griffith right when he reasoned that something from the dead smooth cells of the bacteria was able to transform the rough cells into smooth cells?

Yes

No

No, they did not transform, transduction took place

17. When a virus infects a bacterial cell, it does what?

Replicates its own DNA and the bacteria remains healthy

Replicates itself and destroys bacteria

Replicates its own DNA and degrades the bacterial DNA

18. The uptake of naked DNA by a cell.

Transduction

Conjugation

Transformation

19. Griffiths experiment involved bacteria and mice, with what bacteria?

Streptococcus pneumoniae

Stapholococcus pneumoniae

Bacillicoccus pneumoniae

20. Match the word to its definition: Translation

Messenger RNA directs the synthesis of a protein

DNA makes a mRNA copy of itself during

Messenger RNA directs the synthesis of a protein

21. Can bacteria have sexual relations or sexual iife cycles?

Yes

No

Only sexual life cycles