Gene Expression I: Transcription And Related Stuff

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Gene Expression I: Transcription And Related Stuff

The molecular biology of transcription.


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Questions and Answers
  • 1. 
    RNA polymerase's job is:
    • A. 

      9-5

    • B. 

      Part time

    • C. 

      Weekends only

    • D. 

      Second shift

    • E. 

      Transcription

  • 2. 
    E. coli's RNA polymerase core enzyme does NOT include the subunit called _______.
  • 3. 
    The σ (sigma) subunit of E. coli's RNA polymerase is responsible for RNA polymerase binding to the site on the DNA called the _______.
  • 4. 
    In eukaryotes, there are several RNA polymerases and the one that makes pre-mRNA is RNA polymerase _______. (We didn't cover this one this year, 2010).
  • 5. 
    In eukaryotes, proteins other than RNA polymerase are needed for transcription to occur. Some of these are needed for the transcription of any gene and are called _______ transcription factors. (We didn't cover this one this year, 2010).
  • 6. 
    In eukaryotes, proteins other than RNA polymerase are needed for transcription to occur. Some of these are needed for the transcription of specific genes and are called _______ transcription factors. (We didn't cover this one this year, 2010).
  • 7. 
    In eukaryotes, _______ makes the larger rRNAs. (be specific) (We didn't cover this one this year, 2010).
  • 8. 
    In eukaryotes, _______ makes the smallest rRNAs and the tRNAs. (be specific) (We didn't cover this one this year, 2010).
  • 9. 
    In eukaryotes, the large 45S pre-rRNA molecule is cleaved and produces the ____S rRNA, the 18S rRNA, and the 5.8S rRNA. (We didn't cover this one this year, 2010).
  • 10. 
    In eukaryotes, after about 20-30 nucleotides of the pre-mRNA have been synthesized, a(n) _______ is added to the 5' end of the pre-mRNA.
  • 11. 
    The process of adding about 200 A ribonucleotides onto the 3' end of the pre-mRNA is called ________.
  • 12. 
    _______ of the pre-mRNA stimulates the process of transcription termination. (We didn't cover this one this year, 2010).
  • 13. 
    Splicing removes segments called _______ from the pre-mRNA.
  • 14. 
    As a result of splicing, two segments called _______, which are located on opposite sides of an intron, are joined together.
  • 15. 
    The photo at the top is evidence for the process of _______.
  • 16. 
    RNA processing includes two processes: __________ and __________ (type in both words separated by a space, either order).