Flow Cytometry Training Quiz

13 Questions | Total Attempts: 764

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Flow Cytometry Training Quiz

This quiz will test your knowledge of basic 4-color flow cytometry principles.


Questions and Answers
  • 1. 
    What does light emitted as forward scatter (FSc) measure?
    • A. 

      Cell size

    • B. 

      Cell granularity/complexity

    • C. 

      Cell surface marker fluorescence

  • 2. 
    What does light emitted as side scatter (SSc) measure?
    • A. 

      Cell size

    • B. 

      Cell granularity/complexity

    • C. 

      Cell surface marker fluorescence

  • 3. 
    The FL1 channel measures emission in the green range. What fluorophores does channel 1 (FL1) measure? \ FL1 - Green emission (filter: 530/30)
    • A. 

      FITC, Alexa488, CFSE

    • B. 

      Phycoerythrin (PE)

    • C. 

      Allophycocyannin (APC)

  • 4. 
    The FL2 channel measures emission in the re/orange range. What fluorophores does channel 2 (FL2) measure? FL2 - Red/Orange emission (filter: 585/42)
    • A. 

      FITC, Alexa488, CFSE

    • B. 

      Phycoerythrin (PE)

    • C. 

      PerCP

  • 5. 
    The FL3 channel measures emission in the red range. What fluorophores does channel 3 (FL3) measure? FL3 - Red emission (filter: 650 LP)
    • A. 

      Allophycocyannin (APC)

    • B. 

      PerCP

    • C. 

      FITC, Alexa488, CFSE

  • 6. 
    The FL4 channel measures emission in the far red range. What fluorophores does channel 4 (FL4) measure? FL4 - Far Red emission (filter: - 661 nm)
    • A. 

      FITC, Alexa488, CFSE

    • B. 

      Allophycocyannin (APC)

    • C. 

      PerCP

  • 7. 
    What is the order of brightest fluorophore to weakest fluorophore?
    • A. 

      FL1>FL2>FL3>FL4

    • B. 

      FL4>FL2>FL1>FL3

    • C. 

      FL2>FL3>FL4>FL1

  • 8. 
    What do you have to adjust to make sure emission of fluorescence from a single fluorophore does not bleed into multiple channels?
    • A. 

      Compensation

    • B. 

      Threshold

    • C. 

      Voltage

  • 9. 
    What do you have to adjust to make sure different sized (extremely large or extremely small) cells can be visualized during acquisition?
    • A. 

      Compensation

    • B. 

      Threshold

    • C. 

      Voltage

  • 10. 
    This setting has been previously adjusted on the machine to remove cellular debris from the graph settings during acquisition.
    • A. 

      Compensation

    • B. 

      Threshold

    • C. 

      Voltage

  • 11. 
    What 2 lasers are included in the 4 color BD FACScalibur?
    • A. 

      An argon blue 488nm laser and a red 635 nm diode laser

    • B. 

      An argon red 488nm laser and a blue 635 nm diode laser

    • C. 

      A blue diode 488nm laser and a red 635nm argon laser

  • 12. 
    Place these items in order from first to last for machine shut down. A) Make sure machine is in stand-by mode B) De-pressurize machine using black toggle C) insert tube of ddH2O and put holder all the way to the side for 1 minute D)  insert tube of 10% bleach and put holder all the way to the side for 1 minute E) place holder under tube of bleach and leave for 5 minutes F) place holder under tube of ddH20 and leave for 5 minutes G) leave machine on for 15 minutes in standby mode to let lasers cool down H) remove protective shield from sheath fluid tank I) turn off FACScalibur J) replace protective shield on sheath fluid tank K) set machine to HI setting L) Empty waster container and fill sheath fluid tank
    • A. 

      A>D>E>C>F>K>B>H>L>J>G>I

    • B. 

      K>D>C>E>F>A>B>H>L>J>G>I

    • C. 

      K>D>E>C>F>A>B>H>L>J>G>I

  • 13. 
    What type of graph(s) is used to visualize cells during acquisition?
    • A. 

      Dot plot

    • B. 

      Histogram

    • C. 

      Dot plot and histogram

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