Flow Cytometry Pre-rotation Test

23 Questions | Total Attempts: 585

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Biology Quizzes & Trivia

This is an assessment of your knowledge of flow cytometry. Take it at the beginning of your flow cytometry rotation and again after one week. You can then take until you get a score of 100%. Send results of each score to me.


Questions and Answers
  • 1. 
    • A. 

      Forward angle light scatter

    • B. 

      Right angle light scatter

    • C. 

      Bound antibody surface flourescence

    • D. 

      None of the above

  • 2. 
    Forward angle light scatter is proportional to:
    • A. 

      Cell size

    • B. 

      Nuclear/cytoplasmic complexity

    • C. 

      Ploidy

  • 3. 
    Right angle side scatter is proportional to
    • A. 

      Cell size

    • B. 

      Nuclear/cytoplasmic complexity

    • C. 

      Ploidy

  • 4. 
    The intensity of CD45 flourescence on lymphoblasts is
    • A. 

      Brighter than on mature lymphocytes

    • B. 

      Weaker thanon mature lymphocytes

    • C. 

      The same as on mature lymphocytes

  • 5. 
    The immunophenotype of B cell chronic lymphocytic leukemia includes all of the following except
    • A. 

      CD19

    • B. 

      CD5

    • C. 

      CD23

    • D. 

      CD10

  • 6. 
    The typical flow immunophenotype of mantle cell lymphoma includes all of the following except
    • A. 

      CD19

    • B. 

      CD5

    • C. 

      CD23

    • D. 

      FMC7

  • 7. 
    The typical flow immunophenotype of Burkitt lymphoma includes all of the following except
    • A. 

      CD19

    • B. 

      CD10

    • C. 

      Monoclonal surface light chain

    • D. 

      CD5

  • 8. 
    The typical flow immunophenotype of follicular lymphoma includes all of the following except
    • A. 

      CD19

    • B. 

      CD10

    • C. 

      Monoclonal surface light chain

    • D. 

      CD5

  • 9. 
  • 10. 
    What antigen do we use in flow cytometric analysis to distinguish acute promyelocytic leukemia from other types of acute myeloid leukemia?
    • A. 

      CD13

    • B. 

      CD33

    • C. 

      CD117

    • D. 

      HLA-DR

  • 11. 
    Bone marrow flow cytometric analysis detects a population with the following profile. What is your diagnosis?Positive: CD19, CD20, CD103, CD123, monoclonal surface light chain, with bright expressionNegative: CD5, CD10, CD138
    • A. 

      B cell lymphoma of follicular origin

    • B. 

      Mantle cell lymphoma

    • C. 

      Hairy cell leukemia

    • D. 

      Margina zone B cell lymphoma

  • 12. 
    List three antigens that can be used to distinguish B cell acute lymphoblastic leukemia from Burkitt lymphoma
  • 13. 
    • A. 

      Burkitt lymphoma

    • B. 

      Small lymphocytic lymphoma/CLL

    • C. 

      Marginal zone B cell lymphoma

    • D. 

      Large B cell lymphoma

  • 14. 
    Flow cytometric analysis of bone marrow yielded a dominant population with the following immunophenotype. What is your diagnosis?Positive: CD45 dim, CD7, CD2, CD5, CD4, CD8Negative:CD19, CD20, surface light chain, CD3, HLA-DR
  • 15. 
    The best immunophenotypic profile for identifying T cell lymphoma by flow cytometric analysis is
    • A. 

      Marked predominance of CD4 versus CD8

    • B. 

      Marked predominance of CD8 versus CD4

    • C. 

      Loss of expression of some but not all T cell antigens

    • D. 

      Expression of HLA-DR

  • 16. 
  • 17. 
    List two antigens used in the flow lab to distinguish megakaryocytic differentiation?
  • 18. 
    Per 2008 WHO monograph, what is the most specific marker for identifying myeloid differentiation in acute leukemia?
    • A. 

      CD33

    • B. 

      CD117

    • C. 

      Cytoplasmic myeloperoxidase

    • D. 

      CD13

  • 19. 
    Per 2008 WHO monograph, what is the most specific marker identifying T lymphocytic differentiation in acute leukemia?
    • A. 

      Surface CD7

    • B. 

      Cytoplasmic CD2

    • C. 

      Cytoplasmic CD3

    • D. 

      Surface CD3

  • 20. 
    Flow cytometric analysis detects a intermediate to large cell population with the following immunophenotype. What is your diagnosisNegative: CD45, CD19, CD20, CD3, CD13, CD33, HLA-DRPositive: CD56 bright
  • 21. 
    What subtype of acute myeloid leukemia often aberrantly co-expresses CD19 and CD56, with down regulation of CD33 and CD13?
  • 22. 
    List two antigens and a fluorescent protein that are used for the diagnosis of paroxysmal nocturnal hemoglobinuria by flow cytometric analysis
  • 23. 
    One can distinguish CLL/SLL from monoclonal B cell lymphocytosis by flow immunophenotype.
    • A. 

      True

    • B. 

      False