Flow Cytometry Quiz
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In the following quiz, we’re going to be learning all about the technological advancement that allows us to analyze the physical and chemical characteristics of particles in a fluid while it passes through a laser – that’s right, we’re talking about the topic of flow cytometry. What do you know about this process and the reason why we use it? Take the quiz now to let us know! Good luck!
- 1.What do you have to adjust to make sure emission of fluorescence from a single fluorophore does not bleed into multiple channels?
- A.
Compensation
- B.
Threshold
- C.
Voltage
- 2.What does light emitted as foward side scatter (FSC) measure?
- A.
Cell size
- B.
Cell granularity / complexity
- C.
Cell surface marker fluorescence
- 3.What are the 2 lasers included in the 4 color BD FACSCalibur?
- A.
An argon 488 nm laser and a red 635 nm diode laser
- B.
An argon red 488 nm laser and a blue 635 nm diode laser
- C.
A blue diode 488 nm laser and a red 635 nm argon laser
- 4.The FL4 channel measure emission in the far red range. What fluorophores does channel 4 (FL4) measure? FL4 - far red emission (filter: 661/12 nm)
- A.
FITC, Alexa488, CFSE
- B.
Allophycocyanin (APC)
- C.
PerCP
- 5.The FL1 channel measure emission in the green range. What fluorophores does channel 1 (FL1) measure? FL1 - green emission (filter: 530/30 nm)
- A.
FITC, Alexa488, CFSE
- B.
Phycoerythrin (PE)
- C.
Allophycocyanin (APC)
- 6.The FL3 channel measure emission in the red range. What fluorophores does channel 3 (FL3) measure? FL3 - red emission (filter: 670 nm LP)
- A.
Allophycocyanin (APC)
- B.
PerCP
- C.
FITC, Alexa488, CFSE
- 7.The FL2 channel measure emission in the red orange range. What fluorophores does channel 2 (FL2) measure? FL2 - red orange emission (filter: 585/42 nm)
- A.
FITC, Alexa488, CFSE
- B.
Phycoerythrin (PE)
- C.
PerCP
- 8.What does light emitted as side scatter (SSC) measure?
- A.
Cell size
- B.
Cell granularity / complexity
- C.
Cell surface marker fluorescence
- 9.Which part of the cytometer brings the cells to the interrogation point where the cells meet the laser?
- A.
Electronics
- B.
Fluidics
- C.
Optics
- 10.Which part of the cytometer consists of the excitation sources and detectors?
- A.
Optics
- B.
Electronics
- C.
Fluidics
- 11.Which part of the cytometer converts the light signal to voltage so it can be interpreted through a computer software?
- A.
Fluidics
- B.
Optics
- C.
Electronics
- 12.To which filters those definitions correpond a) trasmit light in the range of specified wavelength, b) transmit light egal or longer than the specified wavelength and c) transmit light equal or shorter than the sepcified wavelength?
- A.
Shortpass, bandpass, longpass
- B.
Longpass, shortpass, bandpass
- C.
Bandpass, longpass, shortpass
- 13.How do you choose a fluorophore?
- A.
By its excitation and emission wavelengths
- B.
By the laser and detectors/filters present in the cytometer
- C.
All answers
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