Flow Cytometry Quiz: Trivia Test!
(139).jpg "Flow Cytometry Quiz: Trivia Test!")
In the following quiz, we’re going to be learning all about the technological advancement that allows us to analyze the physical and chemical characteristics of particles in a fluid while it passes through a laser – that’s right, we’re talking about the topic of flow cytometry. What do you know about this process and the reason why we use it? Take the quiz now to let us know! Good luck!
- 1.What do you have to adjust to make sure the emission of fluorescence from a single fluorophore does not bleed into multiple channels?
- A.
Compensation
- B.
Threshold
- C.
Voltage
- 2.What does light emit as forward side scatter (FSC) measure?
- A.
Cell size
- B.
Cell granularity / complexity
- C.
Cell surface marker fluorescence
- 3.What are the 2 lasers included in the 4 colors BD FACSCalibur?
- A.
An argon 488 nm laser and a red 635 nm diode laser
- B.
An argon red 488 nm laser and a blue 635 nm diode laser
- C.
A blue diode 488 nm laser and a red 635 nm argon laser
- 4.The FL4 channel measures emission in the far red range. What fluorophores does channel 4 (FL4) measure? FL4 - far-red emission (filter: 661/12 nm)
- A.
FITC, Alexa488, CFSE
- B.
Allophycocyanin (APC)
- C.
PerCP
- 5.The FL1 channel measures emission in the green range. What fluorophores does channel 1 (FL1) measure? FL1 - green emission (filter: 530/30 nm)
- A.
FITC, Alexa488, CFSE
- B.
Phycoerythrin (PE)
- C.
Allophycocyanin (APC)
- 6.The FL3 channel measures emission in the red range. What fluorophores does channel 3 (FL3) measure? FL3 - red emission (filter: 670 nm LP)
- A.
Allophycocyanin (APC)
- B.
PerCP
- C.
FITC, Alexa488, CFSE
- 7.The FL2 channel measures emission in the red-orange range. What fluorophores does channel 2 (FL2) measure? FL2 - red-orange emission (filter: 585/42 nm)
- A.
FITC, Alexa488, CFSE
- B.
Phycoerythrin (PE)
- C.
PerCP
- 8.What does light emit as a side scatter (SSC) measure?
- A.
Cell size
- B.
Cell granularity / complexity
- C.
Cell surface marker fluorescence
- 9.Which part of the cytometer brings the cells to the interrogation point where the cells meet the laser?
- A.
Electronics
- B.
Fluidics
- C.
Optics
- 10.Which part of the cytometer consists of the excitation sources and detectors?
- A.
Optics
- B.
Electronics
- C.
Fluidics
- 11.Which part of the cytometer converts the light signal to voltage so it can be interpreted through computer software?
- A.
Fluidics
- B.
Optics
- C.
Electronics
- 12.To which filters those definitions correspond a) transmit light in the range of specified wavelength, b) transmit light equal to or longer than the specified wavelength, and c) transmit light equal to or shorter than the specified wavelength?
- A.
Shortpass, bandpass, longpass
- B.
Longpass, shortpass, bandpass
- C.
Bandpass, longpass, shortpass
- 13.How do you choose a fluorophore?
- A.
By its excitation and emission wavelengths
- B.
By the laser and detectors/filters present in the cytometer
- C.
All answers
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