Final Molecular Exam II

50 Questions | Total Attempts: 101

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Molecule Quizzes & Trivia

Exam II answers


Questions and Answers
  • 1. 
    Which of the conditions does NOt lead to a change in gene expression patterns in bacterial cells?
    • A. 

      Heat shock

    • B. 

      Nutrient availability

    • C. 

      Sporultaion

    • D. 

      Nitrogen deprivation

    • E. 

      None of the choices are correct

  • 2. 
    Which of the following is involved in maintaining lysogeny in phage?
    • A. 

      P RE

    • B. 

      P RO

    • C. 

      P RL

    • D. 

      P RM

    • E. 

      All choices correct

  • 3. 
    One method used by researchers to demonstrate the existence of nucleosome free DNA regions is 
    • A. 

      Nuclear run on experiments

    • B. 

      Reporter gene experiments

    • C. 

      DNase hypersensitivity experiment

    • D. 

      RNA polymerase run off experiments

    • E. 

      Anion exchange chromotography

  • 4. 
    The histone code states that the 
    • A. 

      Lysines are the only amino acids found in histones that can be acetylated and deacylated

    • B. 

      Combination of histone modification on a given nucleosome near a gene's control region affects the efficiency of transcription of that gene

    • C. 

      Combination of histone modification on a given nucleosome near a gene's control region affects the efficiency of transcription of all the nearby genes

    • D. 

      Histones are found in all living cells

  • 5. 
    Following are typical features of transcription activators in eukaryotes?
    • A. 

      Kinase domain

    • B. 

      Transcription-activation domain and kinase domain

    • C. 

      Trasncription- activation domain

    • D. 

      Transcription- activation domain and DNA- binding domain

    • E. 

      DNA-binding domain

  • 6. 
    The glucocortoicoid receptor is activated by binding to 
    • A. 

      Its hormone partner

    • B. 

      DNA and then to its hormone ligand

    • C. 

      Its hormone ligand then moving to the nucleus

    • D. 

      None of the choices are correct

  • 7. 
    The following is not present in the core RNA polymerase of bacteria
    • A. 

      B

    • B. 

      Sigma

    • C. 

      'B

    • D. 

      None of the choices are correct

  • 8. 
    Exp designed to obtain nonspecific transcription from both strands of a DNA molecule in bacteria. which of the following strategies would bee most effective in achieving this?
    • A. 

      Use the core enzyme of RNA polymerase

    • B. 

      Enrich the preparation with sigma subunit

    • C. 

      Include the RNA holoenzyme in the reaction

    • D. 

      Use intact DNA

  • 9. 
    The following is the correct order of addition of general transcription factors to the initiation complex in eukaryotes
    • A. 

      TFIIIA+TFIID, TFIIIIB, TFIIF+pol II, TFIIE, TFIIH

    • B. 

      TFIIA+TFIID, TFIIB, TFIIH,TFIIF +pol II, TFIIE

    • C. 

      TFIIA+TFIID, TFIIB, TFIIE, TFIIF +pol II, TFIIH

    • D. 

      TFIIA+TFIID, TFIIH, TFIIIIB, TFIIF +pol II, TFIIE

  • 10. 
    THE FOLLOWING PLASMIDS COULD BE USED TO RESTORE INDUCIBLE regulation of B galactosidase in this mutant I+Oc Z- Y+ A+
    • A. 

      I+ Oc Z- Y+ A+

    • B. 

      I- O+ Z+ Y+ A+

    • C. 

      I- Oc Z+ Y+ A+

    • D. 

      I+ Oc Z- Y+ A+

  • 11. 
    Which statements is true about a lac operon with this genotype I- d O+ Z+ Y- A-
    • A. 

      Operon is repressible

    • B. 

      Mutation is only cis- dominant

    • C. 

      Operon is uninducible

    • D. 

      Operon is nonrepressible

    • E. 

      None of the choices is correct

  • 12. 
    You are planning an experiment to look at the specific binding of the TATA box binding proteins to the promoter. which would be a suitable negative control?
    • A. 

      DNA sequence made with multiple cytosines and a DNA strand with TATA box linked to multiple adenine

    • B. 

      DNA strand containing the TATA box linked to multiple guanines

    • C. 

      DNA strand containing the TATA box linked to multiple adenine

    • D. 

      A DNA sequence made with multiple cytosines

    • E. 

      All likely

  • 13. 
    Which of the following is true about the holoenzyme of bacterial RNA polymerase in an open complex of bacteria?
    • A. 

      Region 2.4 of the sigma factor binds to the 35 region

    • B. 

      There no interaction between the sigma factor and the 10 region

    • C. 

      DNA is bound mainly to the sigma subunit

    • D. 

      There are two Na+ ions in the core enzyme

  • 14. 
    Which of the following would most likely occur in bacteria if an in vitro transcription assay is performed using cell extract containing an alpha subunit without the C-terminal domain
    • A. 

      RNA polymerase would become tightly associated with the promoter.

    • B. 

      RNA polymerase complex would be loosely associated with the promoter

    • C. 

      RNA polymerase would become more susceptible to proteolysis

    • D. 

      RNA polymerase complex would be loosely associated with the operon

  • 15. 
    Determine which of the following would most likely lead to a dramatic reduction in eukaryotic transcription
    • A. 

      Removal of some TAFs

    • B. 

      Removal of TAFII250

    • C. 

      Absence of the TATA box

    • D. 

      Absence of the TATA box removal of some TAFs

    • E. 

      None are correct

  • 16. 
    New mutant cell line was developed  and was found to be defective in ploymerase III activity of eukaryotes. which is likely to observe the cell line?
    • A. 

      There will be an overabundance of secreted proteins

    • B. 

      There will be overproduction of 7 SL RNA

    • C. 

      Splicing function is impaired

    • D. 

      All likely

  • 17. 
    Which would you choose ti study the role of polymerase subunits in elongation of eukaryotes
    • A. 

      Epitope tagging

    • B. 

      Western blotting

    • C. 

      In vitro transcription

    • D. 

      DNase footprinting

  • 18. 
    Which technique would you use to study role of BRE in eukayrotic promoter region
    • A. 

      Linker scanning mutations

    • B. 

      S1 nuclease protection analysis

    • C. 

      X-Ray crystallography

    • D. 

      Linker scanning mutations and X ray crystallography

  • 19. 
    Predict possible effect of deleting the enhancers region of the polymerase I gene in eukaryotes
    • A. 

      Reduction in production of Rpb 1

    • B. 

      Reduction in amount of rRNA made

    • C. 

      Reduction in production of Rpb2

    • D. 

      Reduced transcription of ribosomes

  • 20. 
    Which of the followin is the correct order chromatin folding
    • A. 

      Nucleosome formation, 30 nM fiber formation, radial loop structure

    • B. 

      Nucelosome formation, radial loop formation, nucleosome formation

    • C. 

      Nucleosome formation, radial loop structure, 30 nM fiber formation

    • D. 

      30 nM fiber formation, nucleosome formation, radial loop formation

  • 21. 
    Studying Xenopus transcription of somatic and oocyte 5S rRNA genes patterns in two types of cells could be seen are between
    • A. 

      RNA Pol III and repressors

    • B. 

      Histones and enhancer elements

    • C. 

      Histones and DNA gyrase

    • D. 

      Histones and transcription factors

  • 22. 
    Which sigma specificity factors is involved in middle gene transcription during SPO I phage infection of bacteria
    • A. 

      Gp33

    • B. 

      Gp34

    • C. 

      Gp43

    • D. 

      Gp24

    • E. 

      Gp28

  • 23. 
    Which is true of early phase of T7 infection of bacteria
    • A. 

      Class I genes are the first to be transcribed

    • B. 

      Class II genes are the first to be transcribed

    • C. 

      Host polymerase transcribes class II genes

    • D. 

      All are correct

  • 24. 
    Which operators is a target of cl gene production of the phage
    • A. 

      O R1

    • B. 

      O L1

    • C. 

      O L2

    • D. 

      O R2

    • E. 

      All are correct

  • 25. 
    Which is true about interaction with repressor and RNA polymerase at P RM?
    • A. 

      Interaction between repressor and polymerase is essential for activation of transcription and interaction involves region of sigma factor

    • B. 

      Interaction involves region 4 sigma factor

    • C. 

      Interaction can be disrupted by binding of CIII

    • D. 

      Interaction between repressor and polymerase is essential for activation of transcription

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