Final Molecular Exam II

50 Questions | By Imasportydiva24 | Updated: Mar 21, 2022 | Attempts: 132

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Exam II answers

Questions and Answers

1.

Which of the conditions does NOt lead to a change in gene expression patterns in bacterial cells?
- A.
  
  Heat shock
- B.
  
  Nutrient availability
- C.
  
  Sporultaion
- D.
  
  Nitrogen deprivation
- E.
  
  None of the choices are correct
2.

Which of the following is involved in maintaining lysogeny in phage?
- A.
  
  P RE
- B.
  
  P RO
- C.
  
  P RL
- D.
  
  P RM
- E.
  
  All choices correct
3.

One method used by researchers to demonstrate the existence of nucleosome free DNA regions is
- A.
  
  Nuclear run on experiments
- B.
  
  Reporter gene experiments
- C.
  
  DNase hypersensitivity experiment
- D.
  
  RNA polymerase run off experiments
- E.
  
  Anion exchange chromotography
4.

The histone code states that the
- A.
  
  Lysines are the only amino acids found in histones that can be acetylated and deacylated
- B.
  
  Combination of histone modification on a given nucleosome near a gene's control region affects the efficiency of transcription of that gene
- C.
  
  Combination of histone modification on a given nucleosome near a gene's control region affects the efficiency of transcription of all the nearby genes
- D.
  
  Histones are found in all living cells
5.

Following are typical features of transcription activators in eukaryotes?
- A.
  
  Kinase domain
- B.
  
  Transcription-activation domain and kinase domain
- C.
  
  Trasncription- activation domain
- D.
  
  Transcription- activation domain and DNA- binding domain
- E.
  
  DNA-binding domain
6.

The glucocortoicoid receptor is activated by binding to
- A.
  
  Its hormone partner
- B.
  
  DNA and then to its hormone ligand
- C.
  
  Its hormone ligand then moving to the nucleus
- D.
  
  None of the choices are correct
7.

The following is not present in the core RNA polymerase of bacteria
- A.
  
  B
- B.
  
  Sigma
- C.
  
  'B
- D.
  
  None of the choices are correct
8.

Exp designed to obtain nonspecific transcription from both strands of a DNA molecule in bacteria. which of the following strategies would bee most effective in achieving this?
- A.
  
  Use the core enzyme of RNA polymerase
- B.
  
  Enrich the preparation with sigma subunit
- C.
  
  Include the RNA holoenzyme in the reaction
- D.
  
  Use intact DNA
9.

The following is the correct order of addition of general transcription factors to the initiation complex in eukaryotes
- A.
  
  TFIIIA+TFIID, TFIIIIB, TFIIF+pol II, TFIIE, TFIIH
- B.
  
  TFIIA+TFIID, TFIIB, TFIIH,TFIIF +pol II, TFIIE
- C.
  
  TFIIA+TFIID, TFIIB, TFIIE, TFIIF +pol II, TFIIH
- D.
  
  TFIIA+TFIID, TFIIH, TFIIIIB, TFIIF +pol II, TFIIE
10.

THE FOLLOWING PLASMIDS COULD BE USED TO RESTORE INDUCIBLE regulation of B galactosidase in this mutant I+Oc Z- Y+ A+
- A.
  
  I+ Oc Z- Y+ A+
- B.
  
  I- O+ Z+ Y+ A+
- C.
  
  I- Oc Z+ Y+ A+
- D.
  
  I+ Oc Z- Y+ A+
11.

Which statements is true about a lac operon with this genotype I- d O+ Z+ Y- A-
- A.
  
  Operon is repressible
- B.
  
  Mutation is only cis- dominant
- C.
  
  Operon is uninducible
- D.
  
  Operon is nonrepressible
- E.
  
  None of the choices is correct
12.

You are planning an experiment to look at the specific binding of the TATA box binding proteins to the promoter. which would be a suitable negative control?
- A.
  
  DNA sequence made with multiple cytosines and a DNA strand with TATA box linked to multiple adenine
- B.
  
  DNA strand containing the TATA box linked to multiple guanines
- C.
  
  DNA strand containing the TATA box linked to multiple adenine
- D.
  
  A DNA sequence made with multiple cytosines
- E.
  
  All likely
13.

Which of the following is true about the holoenzyme of bacterial RNA polymerase in an open complex of bacteria?
- A.
  
  Region 2.4 of the sigma factor binds to the 35 region
- B.
  
  There no interaction between the sigma factor and the 10 region
- C.
  
  DNA is bound mainly to the sigma subunit
- D.
  
  There are two Na+ ions in the core enzyme
14.

Which of the following would most likely occur in bacteria if an in vitro transcription assay is performed using cell extract containing an alpha subunit without the C-terminal domain
- A.
  
  RNA polymerase would become tightly associated with the promoter.
- B.
  
  RNA polymerase complex would be loosely associated with the promoter
- C.
  
  RNA polymerase would become more susceptible to proteolysis
- D.
  
  RNA polymerase complex would be loosely associated with the operon
15.

Determine which of the following would most likely lead to a dramatic reduction in eukaryotic transcription
- A.
  
  Removal of some TAFs
- B.
  
  Removal of TAFII250
- C.
  
  Absence of the TATA box
- D.
  
  Absence of the TATA box removal of some TAFs
- E.
  
  None are correct
16.

New mutant cell line was developed and was found to be defective in ploymerase III activity of eukaryotes. which is likely to observe the cell line?
- A.
  
  There will be an overabundance of secreted proteins
- B.
  
  There will be overproduction of 7 SL RNA
- C.
  
  Splicing function is impaired
- D.
  
  All likely
17.

Which would you choose ti study the role of polymerase subunits in elongation of eukaryotes
- A.
  
  Epitope tagging
- B.
  
  Western blotting
- C.
  
  In vitro transcription
- D.
  
  DNase footprinting
18.

Which technique would you use to study role of BRE in eukayrotic promoter region
- A.
  
  Linker scanning mutations
- B.
  
  S1 nuclease protection analysis
- C.
  
  X-Ray crystallography
- D.
  
  Linker scanning mutations and X ray crystallography
19.

Predict possible effect of deleting the enhancers region of the polymerase I gene in eukaryotes
- A.
  
  Reduction in production of Rpb 1
- B.
  
  Reduction in amount of rRNA made
- C.
  
  Reduction in production of Rpb2
- D.
  
  Reduced transcription of ribosomes
20.

Which of the followin is the correct order chromatin folding
- A.
  
  Nucleosome formation, 30 nM fiber formation, radial loop structure
- B.
  
  Nucelosome formation, radial loop formation, nucleosome formation
- C.
  
  Nucleosome formation, radial loop structure, 30 nM fiber formation
- D.
  
  30 nM fiber formation, nucleosome formation, radial loop formation
21.

Studying Xenopus transcription of somatic and oocyte 5S rRNA genes patterns in two types of cells could be seen are between
- A.
  
  RNA Pol III and repressors
- B.
  
  Histones and enhancer elements
- C.
  
  Histones and DNA gyrase
- D.
  
  Histones and transcription factors
22.

Which sigma specificity factors is involved in middle gene transcription during SPO I phage infection of bacteria
- A.
  
  Gp33
- B.
  
  Gp34
- C.
  
  Gp43
- D.
  
  Gp24
- E.
  
  Gp28
23.

Which is true of early phase of T7 infection of bacteria
- A.
  
  Class I genes are the first to be transcribed
- B.
  
  Class II genes are the first to be transcribed
- C.
  
  Host polymerase transcribes class II genes
- D.
  
  All are correct
24.

Which operators is a target of cl gene production of the phage
- A.
  
  O R1
- B.
  
  O L1
- C.
  
  O L2
- D.
  
  O R2
- E.
  
  All are correct
25.

Which is true about interaction with repressor and RNA polymerase at P RM?
- A.
  
  Interaction between repressor and polymerase is essential for activation of transcription and interaction involves region of sigma factor
- B.
  
  Interaction involves region 4 sigma factor
- C.
  
  Interaction can be disrupted by binding of CIII
- D.
  
  Interaction between repressor and polymerase is essential for activation of transcription