DNA Cloning: An Overview

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DNA Cloning: An Overview - Quiz

DNA CLONING: AN OVERVIEW “Instant Notes in Molecular Biology (Turner et al. , 2005): 105-109Nurul Layyin H


Questions and Answers
  • 1. 
    A wide variety of natural replicons have the properties required to allow them to act as ___________
    • A. 

      DNA ligase

    • B. 

      DNA probe

    • C. 

      Alkaline phosphatase

    • D. 

      Selectable marker

    • E. 

      Cloning vectors

  • 2. 
    Most of the routine manipulations involved in gene cloning use Escherichia coli as _______
    • A. 

      Cloning vectors

    • B. 

      Genomic libraries

    • C. 

      CDNA libraries

    • D. 

      DNA probe

    • E. 

      The host organism

  • 3. 
    A gene which allows host cells containing the vector to be selected from amongst those which do not, usually by conferring resistance to a toxin.
    • A. 

      Selectable marker.

    • B. 

      DNA probe

    • C. 

      Recombinant DNA

    • D. 

      Ti plasmid

    • E. 

      Plasmid

  • 4. 
    An enzyme that joins two double-stranded DNAs end to end.
    • A. 

      DNA ligase

    • B. 

      RNase A

    • C. 

      Alkaline phosphatase

    • D. 

      Reverse transcriptase

    • E. 

      Restriction enzyme

  • 5. 
    A set of clones containing DNA fragments derived directly from a genome.
    • A. 

      Cloning vectors

    • B. 

      Genomic librariy

    • C. 

      CDNA libraries

    • D. 

      DNA probe

    • E. 

      The host organism

  • 6. 
    DNA copies (cDNA) are synthesized from the mRNA by _________
    • A. 

      Subcloning

    • B. 

      Hybridization

    • C. 

      Digestion

    • D. 

      Reverse transcription

    • E. 

      Ligation

  • 7. 
    The joining together of artificially separated nucleic acid molecules via hydrogen bonding between complementary bases.
    • A. 

      Subcloning

    • B. 

      Hybridization

    • C. 

      Digestion

    • D. 

      Reverse transcription

    • E. 

      Ligation

  • 8. 
    Enzymes used in DNA cloning  that removes phosphate from 5′-ends of double- or single-stranded DNA or RNA .
    • A. 

      DNA ligase

    • B. 

      RNase A

    • C. 

      Alkaline phosphatase

    • D. 

      Reverse transcriptase

    • E. 

      Restriction enzyme

  • 9. 
    A labelled molecule used in hybridisation procedures.
    • A. 

      Cloning vector

    • B. 

      Genomic library

    • C. 

      CDNA library

    • D. 

      DNA probe

    • E. 

      Plasmid

  • 10. 
    An endonuclease that cuts DNA at sites defined by its recognition sequence.
    • A. 

      DNA ligase

    • B. 

      RNase A

    • C. 

      Alkaline phosphatase

    • D. 

      Reverse transcriptase

    • E. 

      Restriction enzyme

  • 11. 
    An enzyme that hydrolyses (degrades) single-stranded DNA.
    • A. 

      S1 nuclease

    • B. 

      RNase A

    • C. 

      Alkaline phosphatase

    • D. 

      Reverse transcriptase

    • E. 

      Terminal transferase

  • 12. 
    An enzyme that adds nucleotide residues to the 3' terminus of an oligo- or polynucleotide.
    • A. 

      S1 nuclease

    • B. 

      RNase A

    • C. 

      DNA polymerase I

    • D. 

      Reverse transcriptase

    • E. 

      Terminal transferase

  • 13. 
    Applications of DNA cloning , often collected together under the term genetic engineering, are the following:
    • A. 

      Engineering animals and plants, and gene therapy

    • B. 

      Engineering proteins to alter their properties

    • C. 

      DNA sequencing

    • D. 

      Identification of mutations

    • E. 

      All of the above

  • 14. 
    A collection of clones prepared from the mRNA of a given cell or tissue type,re presenting the genetic information expressed by such cells
    • A. 

      Cloning vectors

    • B. 

      Genomic library

    • C. 

      CDNA library

    • D. 

      DNA probe

    • E. 

      The host organism

  • 15. 
    A  vector for cloning very large pieces of DNA in yeast.
    • A. 

      BAC

    • B. 

      YAC

    • C. 

      Ti plasmid

    • D. 

      Plasmid

    • E. 

      Phage M13

  • 16. 
    An RNA-dependent DNA polymerase found in retroviruses, used in vitro for the synthesis of cDNA.
    • A. 

      S1 nuclease

    • B. 

      RNase A

    • C. 

      DNA polymerase I

    • D. 

      Reverse transcriptase

    • E. 

      Terminal transferase

  • 17. 
    The analysis of the fragmentation of the DNA with restriction enzymes.
    • A. 

      Subcloning

    • B. 

      Hybridization

    • C. 

      Digestion

    • D. 

      Restriction mapping

    • E. 

      Transformation

  • 18. 
    Technique for separating nucleic acid molecules on the basis of their movement through a gel matrix under the influence of an electric field.
    • A. 

      Subcloning

    • B. 

      Hybridization

    • C. 

      Gel electrophoresis

    • D. 

      Ligation

    • E. 

      The polymerase chain reaction

  • 19. 
    Plasmid of Agrobacterium tumefaciens that can be used to integrate DNA into the genome.
    • A. 

      SV40

    • B. 

      YAC

    • C. 

      Ti plasmid

    • D. 

      Baculovirus

    • E. 

      Phage M13

  • 20. 
    An enzyme derived from a thermostable bacterium (Thermus aquaticus). Operates at 72°C and is reasonably stable above 90°C. Used in PCR
    • A. 

      S1 nuclease

    • B. 

      Taq DNA polymerase

    • C. 

      DNA polymerase I

    • D. 

      Reverse transcriptase

    • E. 

      Exonuclease III

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