DNA CLONING: AN OVERVIEW
“Instant Notes in Molecular Biology (Turner et al., 2005): 105-109
Nurul Layyin H
Subcloning
Hybridization
Gel electrophoresis
Ligation
The polymerase chain reaction
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DNA ligase
RNase A
Alkaline phosphatase
Reverse transcriptase
Restriction enzyme
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Subcloning
Hybridization
Digestion
Reverse transcription
Ligation
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S1 nuclease
Taq DNA polymerase
DNA polymerase I
Reverse transcriptase
Exonuclease III
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Engineering animals and plants, and gene therapy
Engineering proteins to alter their properties
DNA sequencing
Identification of mutations
All of the above
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Selectable marker.
DNA probe
Recombinant DNA
Ti plasmid
Plasmid
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Cloning vector
Genomic library
CDNA library
DNA probe
Plasmid
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Cloning vectors
Genomic libraries
CDNA libraries
DNA probe
The host organism
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Subcloning
Hybridization
Digestion
Restriction mapping
Transformation
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DNA ligase
DNA probe
Alkaline phosphatase
Selectable marker
Cloning vectors
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DNA ligase
RNase A
Alkaline phosphatase
Reverse transcriptase
Restriction enzyme
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BAC
YAC
Ti plasmid
Plasmid
Phage M13
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SV40
YAC
Ti plasmid
Baculovirus
Phage M13
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S1 nuclease
RNase A
DNA polymerase I
Reverse transcriptase
Terminal transferase
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Cloning vectors
Genomic librariy
CDNA libraries
DNA probe
The host organism
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Cloning vectors
Genomic library
CDNA library
DNA probe
The host organism
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S1 nuclease
RNase A
Alkaline phosphatase
Reverse transcriptase
Terminal transferase
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Subcloning
Hybridization
Digestion
Reverse transcription
Ligation
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S1 nuclease
RNase A
DNA polymerase I
Reverse transcriptase
Terminal transferase
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