1 to 6C
1 to 10C
20 to 24C
37C
Citrate-phosphate-dextrose (CPD): 35 days
Additive solution (AS): 47 days
Citrate-phosphate-dextrose-adenine (CPDA)-1: 21 days
Acid-citrate-dextrose (ACD): 35 days
Citrate-phosphate-dextrose-dextrose (CP2D): 21 days
Percentage of viable cells at 24 hours after transfusion decreases to 71%
Supernatant K+ (potassium ion) concentration decreases
Supernatant pH increases
Red cell 2, 3-DPG increases
Supernatant hemoglobin decreases
10 minutes
30 minutes
1 hour
4 hours
6 hours
4 hours
6 hours
24 hours
21 days
28 days
Thawing and deglycerolizing a frozen unit
Filtering using a leukocyte-reduction filter
Irradiation
Centrifugation
Washing
5%
10%
20%
40%
65%
Glycerol is not approved by the FDA
Glycerol is toxic to kidneys
Glycerol can cause hemolysis
Glycerol can cause anaphylaxis
Glycerol can cause thrombocytopenia
Inadequate deglycerolization
Bacterial contamination
Insufficient anticoagulant
Inadvertent use of hypotonic saline for washing
Red cells from a donor with sickle cell trait
Refrigerate the units until the expiration time and discard if not clinically needed
Advise the medical staff to transfuse the units because they are rare
Document the value of the antigen-negative units and refreeze for up to another 10 years
Release the rare units so that they can be made available for another patient
1 hour
4 hours
8 hours
24 hours
36 hours
5.5 x 10^ 9
5.5 x 10 ^ 10
5.5 x 10 ^11
3 x 10 ^ 10
3 x 10 ^ 11
4.2
5.2
6.2
7.2
8.2
Decreased H+ concentration
Platelet activation
Change in shape from round to discoid
Increased swirling effect
Increased expression of glycoprotein Ib and glycoprotein IIb/IIIa
1000 to 5000 /uL
3000 to 5000 /uL
3000 to 12000 /uL
5000 to 10000/ uL
30000 to 40000 .uL
Decreased donor exposure for infectious disease transmission
Decreased risk of TRALI
Decreased donor exposure for HLA alloimmunization
Decreased risk of septic transfusion reaction
All of the above
4 hours
6 hours
12 hours
18 hours
24 hours
To prepare FFP plasma must be separated from red cells within 24 hours
If an additive solution is used, the expiration date for RBCs stored at 1 to 6 C is 42 days after phlebotomy
To prepare Cryo FFP is thawed at 20 to 24 C
Platelets derived from a unit of whole blood must contain 3 x 10 ^ 11 platelets in 75% of units released
The expiration date of RBCs that are frozen and stored at
Group O FFP
Group B, Rh positive cryo
Group A FFP
Group AB FFP
Group A, Rh negative cryo
Cryo can be made from cryo-reduced plasma (CRP) or FFP
CRP can not be used in manufacturing albumin and immunoglobulins
CRP and cryo can not be prepared from FFP collected by apheresis
CRP is rich in fibrinogen, factor VIII, and vWF
CRP is deficient in ADAMTS13
1 to 6 C
20 to 22C
30 C
37C
42C
37C
20 to 24 C
1 to 6 C
-18 C
70
80
100
120
150
Factor XI
Protein C
Protein S
Factor XIII
Factor XII
Is prepared from FFP thawed at 20 to 24C
Once thawed is stored at 1 to 6 C and given within 6 hours of thawing or 4 hours of pooling
It is prepared by filtering thawed FFP at 1 to 6 C
It is prepared by centrifuging thawed FFP at 1 to 6 C
Thawed cryo is stored at 1 to 6 C during transport