Quiz Online 2 Working With Nucleic Acids

18 Questions | By Faunsoed | Last updated: Mar 18, 2022 | Total Attempts: 280

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1.

The biology of gene manipulation requires facilities for the growth, containment, and processing of different types of cells and organisms.
- A.
  
  TRUE
- B.
  
  FALSE
2.

Which of the following is NOT basic requirements for isolating nucleic acids?
- A.
  
  Opening the cells in the sample to expose the nucleic acids for further processing
- B.
  
  Separation of the nucleic acids from other cell components
- C.
  
  Recovery of the nucleic acid in purified form
- D.
  
  Labelling of nucleic acids
3.

If a DNA preparation is required, which of the following enzyme can be used to digest the RNA in the preparation?
- A.
  
  Ribonuclease (RNase)
- B.
  
  Polynucleotide kinase
- C.
  
  DNA polymerase I
- D.
  
  Deoxyribonuclease I (DNase I)
4.

The concentration of a solution of nucleic acid can be determined by measuring the absorbance at ________.
- A.
  
  280 nm, using a spectrophotometer
- B.
  
  260 nm, using a spectrophotometer
- C.
  
  260 nm, using a DNA sequencers
- D.
  
  280 nm, using a DNA sequencers
5.

Which of the following chemical is required for a deproteinisation stage?
- A.
  
  Phenol
- B.
  
  Ethanol
- C.
  
  Methanol
- D.
  
  Isopropanol
6.

The technique of gradient centrifugation is often used to prepare ____________.
- A.
  
  MRNA
- B.
  
  TRNA
- C.
  
  CDNA
- D.
  
  DNA
7.

The concentration of DNA can be estimated by monitoring the fluorescence of _________ that binds between the DNA bases (intercalates).
- A.
  
  Ethidium bromide (EtBr)
- B.
  
  Calcium chloride (CaCl2)
- C.
  
  Sulphur-35 (35S)
- D.
  
  Phosphorus-32 (32P)
8.

In the end labelling technique, the enzyme __________ is used to transfer the terminal phosphate group of ATP onto 5’-hydroxyl termini of nucleic acid molecules.
- A.
  
  Ribonuclease (RNase)
- B.
  
  Polynucleotide kinase
- C.
  
  DNA polymerase I
- D.
  
  Deoxyribonuclease I (DNase I)
9.

Nick translation relies on the ability of the enzyme ______________ to translate (move along the DNA) a nick created in the phosphodiester backbone of the DNA double helix
- A.
  
  Ribonuclease (RNase)
- B.
  
  Polynucleotide kinase
- C.
  
  DNA polymerase I
- D.
  
  Deoxyribonuclease I (DNase I)
10.

Precipitation of nucleic acids is an essential technique that is used in a variety of applications. The two most commonly used precipitants are _______________
- A.
  
  Phenol and methanol
- B.
  
  Phenol and isopropanol
- C.
  
  Alcohol and methanol
- D.
  
  Isopropanol and ethanol
11.

Separation of charged molecules are separated based on varying rates of migration through a solid matrix when subjected to an electric field.
- A.
  
  Affinity chromatography
- B.
  
  Spectrophotometer
- C.
  
  Automated DNA sequencers
- D.
  
  Gel electrophoresis
12.

There are several variants of the basic DNA sequencing procedure, but the most widely used techniques are based on ___________
- A.
  
  Affinity chromatography
- B.
  
  Spectrophotometer
- C.
  
  The enzymatic method
- D.
  
  The chemical method
13.

Separation of the DNA fragments created in sequencing reactions is achieved by________
- A.
  
  Affinity chromatography
- B.
  
  Electrophoresis
- C.
  
  PAGE
- D.
  
  SAGE
14.

When added to a DNA solution in a ratio, by volume, of 2:1 in the presence of 0.2 M salt, ethanol causes the nucleic acids to come out of solution.
- A.
  
  TRUE
- B.
  
  FALSE
15.

Nicks may occur naturally or may be caused by a low concentration of the nuclease DNase I in the reaction mixture.
- A.
  
  TRUE
- B.
  
  FALSE
16.

DNA polymerase I catalyses a strand-replacement reaction that incorporates new dNTPs into the DNA chain.
- A.
  
  TRUE
- B.
  
  FALSE
17.

Labelling by primer extension refers to a technique that uses random oligonucleotides to prime synthesis of a DNA strand by DNA polymerase.
- A.
  
  TRUE
- B.
  
  FALSE
18.

Application of radiolabelling is in the production of highly radioactive nucleic acid molecules for use in hybridisation experiments.
- A.
  
  TRUE
- B.
  
  FALSE