This quiz covers key aspects of working with nucleic acids, focusing on gene manipulation, isolation requirements, enzymatic treatments, and analytical techniques.
TRUE
FALSE
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TRUE
FALSE
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Ribonuclease (RNase)
Polynucleotide kinase
DNA polymerase I
Deoxyribonuclease I (DNase I)
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TRUE
FALSE
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TRUE
FALSE
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Ethidium bromide (EtBr)
Calcium chloride (CaCl2)
Sulphur-35 (35S)
Phosphorus-32 (32P)
Affinity chromatography
Spectrophotometer
Automated DNA sequencers
Gel electrophoresis
Opening the cells in the sample to expose the nucleic acids for further processing
Separation of the nucleic acids from other cell components
Recovery of the nucleic acid in purified form
Labelling of nucleic acids
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Ribonuclease (RNase)
Polynucleotide kinase
DNA polymerase I
Deoxyribonuclease I (DNase I)
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FALSE
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280 nm, using a spectrophotometer
260 nm, using a spectrophotometer
260 nm, using a DNA sequencers
280 nm, using a DNA sequencers
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Ribonuclease (RNase)
Polynucleotide kinase
DNA polymerase I
Deoxyribonuclease I (DNase I)
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Phenol
Ethanol
Methanol
Isopropanol
Affinity chromatography
Spectrophotometer
The enzymatic method
The chemical method
MRNA
TRNA
CDNA
DNA
Phenol and methanol
Phenol and isopropanol
Alcohol and methanol
Isopropanol and ethanol
Affinity chromatography
Electrophoresis
PAGE
SAGE
Quiz Review Timeline (Updated): Mar 18, 2023 +
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