Quiz Online 2 Working With Nucleic Acids

18 Questions | Total Attempts: 280

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Quiz Online 2 Working With Nucleic Acids - Quiz


Questions and Answers
  • 1. 
    The biology of gene manipulation requires facilities for the growth, containment, and processing of different types of cells and organisms.
    • A. 

      TRUE

    • B. 

      FALSE

  • 2. 
    Which of the following is NOT basic requirements for isolating nucleic acids?
    • A. 

      Opening the cells in the sample to expose the nucleic acids for further processing

    • B. 

      Separation of the nucleic acids from other cell components

    • C. 

      Recovery of the nucleic acid in purified form

    • D. 

      Labelling of nucleic acids

  • 3. 
    If a DNA preparation is required, which of the following enzyme can be used to digest the RNA in the preparation?
    • A. 

      Ribonuclease (RNase)

    • B. 

      Polynucleotide kinase

    • C. 

      DNA polymerase I

    • D. 

      Deoxyribonuclease I (DNase I)

  • 4. 
    The concentration of a solution of nucleic acid can be determined by measuring the absorbance at ________.
    • A. 

      280 nm, using a spectrophotometer

    • B. 

      260 nm, using a spectrophotometer

    • C. 

      260 nm, using a DNA sequencers

    • D. 

      280 nm, using a DNA sequencers

  • 5. 
    Which of the following chemical is required for a deproteinisation stage?
    • A. 

      Phenol

    • B. 

      Ethanol

    • C. 

      Methanol

    • D. 

      Isopropanol

  • 6. 
    The technique of gradient centrifugation is often used to prepare ____________.
    • A. 

      MRNA

    • B. 

      TRNA

    • C. 

      CDNA

    • D. 

      DNA

  • 7. 
    The concentration of DNA can be estimated by monitoring the fluorescence of _________ that binds between the DNA bases (intercalates).
    • A. 

      Ethidium bromide (EtBr)

    • B. 

      Calcium chloride (CaCl2)

    • C. 

      Sulphur-35 (35S)

    • D. 

      Phosphorus-32 (32P)

  • 8. 
    In the end labelling technique, the enzyme __________ is used to transfer the terminal phosphate group of ATP onto 5’-hydroxyl termini of nucleic acid molecules.
    • A. 

      Ribonuclease (RNase)

    • B. 

      Polynucleotide kinase

    • C. 

      DNA polymerase I

    • D. 

      Deoxyribonuclease I (DNase I)

  • 9. 
    Nick translation relies on the ability of the enzyme ______________ to translate (move along the DNA) a nick created in the phosphodiester backbone of the DNA double helix
    • A. 

      Ribonuclease (RNase)

    • B. 

      Polynucleotide kinase

    • C. 

      DNA polymerase I

    • D. 

      Deoxyribonuclease I (DNase I)

  • 10. 
    Precipitation of nucleic acids is an essential technique that is used in a variety of applications. The two most commonly used precipitants are ­­­­­­­­­­­­­_______________
    • A. 

      Phenol and methanol

    • B. 

      Phenol and isopropanol

    • C. 

      Alcohol and methanol

    • D. 

      Isopropanol and ethanol

  • 11. 
    Separation of charged molecules are separated based on varying rates of migration through a solid matrix when subjected to an electric field.
    • A. 

      Affinity chromatography

    • B. 

      Spectrophotometer

    • C. 

      Automated DNA sequencers

    • D. 

      Gel electrophoresis

  • 12. 
    There are several variants of the basic DNA sequencing procedure, but the most widely used techniques are based on ___________
    • A. 

      Affinity chromatography

    • B. 

      Spectrophotometer

    • C. 

      The enzymatic method

    • D. 

      The chemical method

  • 13. 
    Separation of the DNA fragments created in sequencing reactions is achieved by________
    • A. 

      Affinity chromatography

    • B. 

      Electrophoresis

    • C. 

      PAGE

    • D. 

      SAGE

  • 14. 
    When added to a DNA solution in a ratio, by volume, of 2:1 in the presence of 0.2 M salt, ethanol causes the nucleic acids to come out of solution.
    • A. 

      TRUE

    • B. 

      FALSE

  • 15. 
    Nicks may occur naturally or may be caused by a low concentration of the nuclease DNase I in the reaction mixture.
    • A. 

      TRUE

    • B. 

      FALSE

  • 16. 
    DNA polymerase I catalyses a strand-replacement reaction that incorporates new dNTPs into the DNA chain.
    • A. 

      TRUE

    • B. 

      FALSE

  • 17. 
    Labelling by primer extension refers to a technique that uses random oligonucleotides to prime synthesis of a DNA strand by DNA polymerase.
    • A. 

      TRUE

    • B. 

      FALSE

  • 18. 
    Application of radiolabelling is in the production of highly radioactive nucleic acid molecules for use in hybridisation experiments.
    • A. 

      TRUE

    • B. 

      FALSE

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