Quiz Online 2 Working With Nucleic Acids
- 1.The biology of gene manipulation requires facilities for the growth, containment, and processing of different types of cells and organisms.
- A.
TRUE
- B.
FALSE
- 2.Which of the following is NOT basic requirements for isolating nucleic acids?
- A.
Opening the cells in the sample to expose the nucleic acids for further processing
- B.
Separation of the nucleic acids from other cell components
- C.
Recovery of the nucleic acid in purified form
- D.
Labelling of nucleic acids
- 3.If a DNA preparation is required, which of the following enzyme can be used to digest the RNA in the preparation?
- A.
Ribonuclease (RNase)
- B.
Polynucleotide kinase
- C.
DNA polymerase I
- D.
Deoxyribonuclease I (DNase I)
- 4.The concentration of a solution of nucleic acid can be determined by measuring the absorbance at ________.
- A.
280 nm, using a spectrophotometer
- B.
260 nm, using a spectrophotometer
- C.
260 nm, using a DNA sequencers
- D.
280 nm, using a DNA sequencers
- 5.Which of the following chemical is required for a deproteinisation stage?
- A.
Phenol
- B.
Ethanol
- C.
Methanol
- D.
Isopropanol
- 6.The technique of gradient centrifugation is often used to prepare ____________.
- A.
MRNA
- B.
TRNA
- C.
CDNA
- D.
DNA
- 7.The concentration of DNA can be estimated by monitoring the fluorescence of _________ that binds between the DNA bases (intercalates).
- A.
Ethidium bromide (EtBr)
- B.
Calcium chloride (CaCl2)
- C.
Sulphur-35 (35S)
- D.
Phosphorus-32 (32P)
- 8.In the end labelling technique, the enzyme __________ is used to transfer the terminal phosphate group of ATP onto 5’-hydroxyl termini of nucleic acid molecules.
- A.
Ribonuclease (RNase)
- B.
Polynucleotide kinase
- C.
DNA polymerase I
- D.
Deoxyribonuclease I (DNase I)
- 9.Nick translation relies on the ability of the enzyme ______________ to translate (move along the DNA) a nick created in the phosphodiester backbone of the DNA double helix
- A.
Ribonuclease (RNase)
- B.
Polynucleotide kinase
- C.
DNA polymerase I
- D.
Deoxyribonuclease I (DNase I)
- 10.Precipitation of nucleic acids is an essential technique that is used in a variety of applications. The two most commonly used precipitants are _______________
- A.
Phenol and methanol
- B.
Phenol and isopropanol
- C.
Alcohol and methanol
- D.
Isopropanol and ethanol
- 11.Separation of charged molecules are separated based on varying rates of migration through a solid matrix when subjected to an electric field.
- A.
Affinity chromatography
- B.
Spectrophotometer
- C.
Automated DNA sequencers
- D.
Gel electrophoresis
- 12.There are several variants of the basic DNA sequencing procedure, but the most widely used techniques are based on ___________
- A.
Affinity chromatography
- B.
Spectrophotometer
- C.
The enzymatic method
- D.
The chemical method
- 13.Separation of the DNA fragments created in sequencing reactions is achieved by________
- A.
Affinity chromatography
- B.
Electrophoresis
- C.
PAGE
- D.
SAGE
- 14.When added to a DNA solution in a ratio, by volume, of 2:1 in the presence of 0.2 M salt, ethanol causes the nucleic acids to come out of solution.
- A.
TRUE
- B.
FALSE
- 15.Nicks may occur naturally or may be caused by a low concentration of the nuclease DNase I in the reaction mixture.
- A.
TRUE
- B.
FALSE
- 16.DNA polymerase I catalyses a strand-replacement reaction that incorporates new dNTPs into the DNA chain.
- A.
TRUE
- B.
FALSE
- 17.Labelling by primer extension refers to a technique that uses random oligonucleotides to prime synthesis of a DNA strand by DNA polymerase.
- A.
TRUE
- B.
FALSE
- 18.Application of radiolabelling is in the production of highly radioactive nucleic acid molecules for use in hybridisation experiments.
- A.
TRUE
- B.
FALSE
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