Molecular Biology Quiz (Lectures 11 & 12)
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- 1.Southern Blots are used to study...
- A.
DNA only.
- B.
RNA only.
- C.
Proteins only.
- D.
DNA and RNA only.
- E.
DNA and Proteins only.
- F.
RNA and Proteins only.
- G.
DNA, RNA, and Proteins.
- 2.Which is the correct order for steps to be taken in a Southern Blot? I. Detect specific fragments by hybridizing DNA probe (radioactive or non-radioactive) to DNA on membrane. II. Denature dsDNA in gel using NaOH (high pH). III. Digest DNA with restriction enzyme. IV. Visualize hybridized bands with P-imager or film. V. Transfer ("blot") ssDNA from gel to membrane. VI. Size fractionate DNA fragments in agarose gel and stain with EtBr to visualize all DNA.
- A.
III, VI, II, V, I, IV
- B.
II, IV, V, VI, III, I
- C.
IV, I, V, II, VI, III
- D.
III, I, V, VI, II, IV
- 3.What type of blot is used to detect a Restriction Fragment Length Polymorphism (RFLP), in order to analyze genetic variation?
- 4.Northern Blots are used to study...
- A.
DNA only.
- B.
RNA only.
- C.
Proteins only.
- D.
DNA and RNA only.
- E.
DNA and Proteins only.
- F.
RNA and Proteins only.
- G.
DNA, RNA, and Proteins.
- 5.
- A.
Northern Blots do not require restriction endonucleases, but the substance being studied must be denatured in heated formaldehyde, in stead of SDS.
- B.
Southern Blots do not require restriction endonucleases, but the substance being studied must be denatured in heated formaldehyde, in stead of SDS.
- C.
Northern Blots require restriction endonucleases, and the substance being studied must be denatured in heated formaldehyde, in stead of SDS.
- D.
Southern Blots require restriction endonucleases, and the substance being studied must be denatured in heated formaldehyde, in stead of SDS.
- 6.Sanger method of Sequencing DNA description: ________, _____ _____________.
- 7.Cycle sequencing is...
- A.
Is an improvement on Sanger method
- B.
Allows automation.
- C.
Includes "454" and "illumina" methods.
- 8.Check all that are true with regards to Sanger Sequencing.
- A.
Syntehsizes new strand of DNA in vitro.
- B.
Includes dideoxy nucleotides (ddNTP) that terminate DNA synthesis.
- C.
Each tube has known dideoxy nt (ddATP, ddGTP, ddTTP, or ddCTP).
- 9.Differences between Automated Sequencing and Sanger Sequencing:
- A.
DNA labeled with fluorescent dyes instead of (^35)S.
- B.
Dyes are on Dideoxynucleotides(ddNTPs).
- C.
DNA is electrophoresed in polyacrylamide gel.
- D.
DNA labeled with (^35)S instead of fluorescent dyes.
- E.
Dyes are on deoxynucleotides (dNTPs).
- F.
DNA is electrophoresed in agarose gel.
- G.
Detected by exciting dye with laser.
- H.
Not detected by exciting dye with laser.
- I.
Uses one color, and four lanes, one for each nucleotide, for detection.
- J.
Uses one lane, and four colors, one for each nucleotide, for detection.
- 10.Select those of the following that are true about "Next Generation" sequencing methods:
- A.
"2nd Generation" Sequencing includes "454" Pyrosequencing and Illumina.
- B.
"2nd Generation" Sequencing includes "454" Pyrosequencing only.
- C.
"2nd Generation" Sequencing includes Illumina only.
- D.
"3rd Generation" Sequencing includes "454" Pyrosequencing and Illumina.
- E.
"3rd Generation" Sequencing includes "454" Pyrosequencing only.
- F.
"3rd Generation" Sequencing includes Illumina only.
- G.
"2nd Generation" Sequencing includes single molecule sequencing.
- H.
"3rd Generation" Sequencing includes single molecule sequencing.
- 11.The main benefit(s) of improvements on sequencing methods is(are)
- A.
Cost by size of molecule being sequenced is lowering.
- B.
Speed of sequencing is rising.
- C.
Cost by size of molecule being sequenced is rising.
- D.
Speed of sequencing is lowering.
- 12.Which of the following are true about "454" Pyrosequencing?
- A.
It involves a combustion reaction at a molecular level.
- B.
Based on the release of "Pyrophosphate" (P-P) molecule.
- C.
Generates relatively long "reads"(~400bp)
- D.
Generates relatively short "reads"(~40bp)
- E.
Can sequence ~500Mbp DNA/day
- F.
Can sequence ~1000Mbp DNA/day
- 13.Reporter Genes are:
- A.
"Synthetic" and used to study function of regulatory sequences (promoters, RNA stability, translation regulation, etc.).
- B.
"Natural" and used to study function of regulatory sequences (promoters, RNA stability, translation regulation, etc.).
- C.
Includes "regulatory sequence" from gene of interest.
- D.
Add a coding region for specific "Reporter Protein," one that is the same with an experiment.
- E.
Add a coding region for random "Reporter Protein," one that is the same with an experiment.
- F.
Add a coding region for specific "Reporter Protein," one that is different with an experiment.
- G.
Add a coding region for random "Reporter Protein," one that is different with an experiment.
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