Molecular Biology Quiz (Lectures 11 & 12)

14 Questions

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Molecular Biology Quizzes & Trivia

Questions and Answers
  • 1. 
    Southern Blots are used to study...
    • A. 

      DNA only.

    • B. 

      RNA only.

    • C. 

      Proteins only.

    • D. 

      DNA and RNA only.

    • E. 

      DNA and Proteins only.

    • F. 

      RNA and Proteins only.

    • G. 

      DNA, RNA, and Proteins.

  • 2. 
    Which is the correct order for steps to be taken in a Southern Blot? I.    Detect specific fragments by hybridizing DNA probe (radioactive or non-radioactive) to DNA on membrane. II.   Denature dsDNA in gel using NaOH (high pH). III.  Digest DNA with restriction enzyme. IV.  Visualize hybridized bands with P-imager or film. V.   Transfer ("blot") ssDNA from gel to membrane. VI.  Size fractionate DNA fragments in agarose gel and stain with EtBr to visualize all DNA.
    • A. 

      III, VI, II, V, I, IV

    • B. 

      II, IV, V, VI, III, I

    • C. 

      IV, I, V, II, VI, III

    • D. 

      III, I, V, VI, II, IV

  • 3. 
    What type of blot is used to detect a Restriction Fragment Length Polymorphism (RFLP), in order to analyze genetic variation?
  • 4. 
    Northern Blots are used to study...
    • A. 

      DNA only.

    • B. 

      RNA only.

    • C. 

      Proteins only.

    • D. 

      DNA and RNA only.

    • E. 

      DNA and Proteins only.

    • F. 

      RNA and Proteins only.

    • G. 

      DNA, RNA, and Proteins.

  • 5. 
    • A. 

      Northern Blots do not require restriction endonucleases, but the substance being studied must be denatured in heated formaldehyde, in stead of SDS.

    • B. 

      Southern Blots do not require restriction endonucleases, but the substance being studied must be denatured in heated formaldehyde, in stead of SDS.

    • C. 

      Northern Blots require restriction endonucleases, and the substance being studied must be denatured in heated formaldehyde, in stead of SDS.

    • D. 

      Southern Blots require restriction endonucleases, and the substance being studied must be denatured in heated formaldehyde, in stead of SDS.

  • 6. 
    Sanger method of Sequencing DNA description:  ________,  _____   _____________.
  • 7. 
    Cycle sequencing is...
    • A. 

      Is an improvement on Sanger method

    • B. 

      Allows automation.

    • C. 

      Includes "454" and "illumina" methods.

  • 8. 
    Check all that are true with regards to Sanger Sequencing.
    • A. 

      Syntehsizes new strand of DNA in vitro.

    • B. 

      Includes dideoxy nucleotides (ddNTP) that terminate DNA synthesis.

    • C. 

      Each tube has known dideoxy nt (ddATP, ddGTP, ddTTP, or ddCTP).

  • 9. 
    Differences between Automated Sequencing and Sanger Sequencing:
    • A. 

      DNA labeled with fluorescent dyes instead of (^35)S.

    • B. 

      Dyes are on Dideoxynucleotides(ddNTPs).

    • C. 

      DNA is electrophoresed in polyacrylamide gel.

    • D. 

      DNA labeled with (^35)S instead of fluorescent dyes.

    • E. 

      Dyes are on deoxynucleotides (dNTPs).

    • F. 

      DNA is electrophoresed in agarose gel.

    • G. 

      Detected by exciting dye with laser.

    • H. 

      Not detected by exciting dye with laser.

    • I. 

      Uses one color, and four lanes, one for each nucleotide, for detection.

    • J. 

      Uses one lane, and four colors, one for each nucleotide, for detection.

  • 10. 
    Select those of the following that are true about "Next Generation" sequencing methods:
    • A. 

      "2nd Generation" Sequencing includes "454" Pyrosequencing and Illumina.

    • B. 

      "2nd Generation" Sequencing includes "454" Pyrosequencing only.

    • C. 

      "2nd Generation" Sequencing includes Illumina only.

    • D. 

      "3rd Generation" Sequencing includes "454" Pyrosequencing and Illumina.

    • E. 

      "3rd Generation" Sequencing includes "454" Pyrosequencing only.

    • F. 

      "3rd Generation" Sequencing includes Illumina only.

    • G. 

      "2nd Generation" Sequencing includes single molecule sequencing.

    • H. 

      "3rd Generation" Sequencing includes single molecule sequencing.

  • 11. 
    The main benefit(s) of improvements on sequencing methods is(are)
    • A. 

      Cost by size of molecule being sequenced is lowering.

    • B. 

      Speed of sequencing is rising.

    • C. 

      Cost by size of molecule being sequenced is rising.

    • D. 

      Speed of sequencing is lowering.

  • 12. 
    Which of the following are true about "454" Pyrosequencing?
    • A. 

      It involves a combustion reaction at a molecular level.

    • B. 

      Based on the release of "Pyrophosphate" (P-P) molecule.

    • C. 

      Generates relatively long "reads"(~400bp)

    • D. 

      Generates relatively short "reads"(~40bp)

    • E. 

      Can sequence ~500Mbp DNA/day

    • F. 

      Can sequence ~1000Mbp DNA/day

  • 13. 
    Reporter Genes are:
    • A. 

      "Synthetic" and used to study function of regulatory sequences (promoters, RNA stability, translation regulation, etc.).

    • B. 

      "Natural" and used to study function of regulatory sequences (promoters, RNA stability, translation regulation, etc.).

    • C. 

      Includes "regulatory sequence" from gene of interest.

    • D. 

      Add a coding region for specific "Reporter Protein," one that is the same with an experiment.

    • E. 

      Add a coding region for random "Reporter Protein," one that is the same with an experiment.

    • F. 

      Add a coding region for specific "Reporter Protein," one that is different with an experiment.

    • G. 

      Add a coding region for random "Reporter Protein," one that is different with an experiment.