This Molecular Biology Quiz covers key techniques such as Southern and Northern blotting, and DNA sequencing. It assesses understanding of molecular genetics, specifically DNA and RNA analysis, and genetic variation through practical applications of these techniques.
III, VI, II, V, I, IV
II, IV, V, VI, III, I
IV, I, V, II, VI, III
III, I, V, VI, II, IV
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DNA only.
RNA only.
Proteins only.
DNA and RNA only.
DNA and Proteins only.
RNA and Proteins only.
DNA, RNA, and Proteins.
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Northern Blots do not require restriction endonucleases, but the substance being studied must be denatured in heated formaldehyde, in stead of SDS.
Southern Blots do not require restriction endonucleases, but the substance being studied must be denatured in heated formaldehyde, in stead of SDS.
Northern Blots require restriction endonucleases, and the substance being studied must be denatured in heated formaldehyde, in stead of SDS.
Southern Blots require restriction endonucleases, and the substance being studied must be denatured in heated formaldehyde, in stead of SDS.
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Is an improvement on Sanger method
Allows automation.
Includes "454" and "illumina" methods.
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Syntehsizes new strand of DNA in vitro.
Includes dideoxy nucleotides (ddNTP) that terminate DNA synthesis.
Each tube has known dideoxy nt (ddATP, ddGTP, ddTTP, or ddCTP).
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DNA labeled with fluorescent dyes instead of (^35)S.
Dyes are on Dideoxynucleotides(ddNTPs).
DNA is electrophoresed in polyacrylamide gel.
DNA labeled with (^35)S instead of fluorescent dyes.
Dyes are on deoxynucleotides (dNTPs).
DNA is electrophoresed in agarose gel.
Detected by exciting dye with laser.
Not detected by exciting dye with laser.
Uses one color, and four lanes, one for each nucleotide, for detection.
Uses one lane, and four colors, one for each nucleotide, for detection.
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"2nd Generation" Sequencing includes "454" Pyrosequencing and Illumina.
"2nd Generation" Sequencing includes "454" Pyrosequencing only.
"2nd Generation" Sequencing includes Illumina only.
"3rd Generation" Sequencing includes "454" Pyrosequencing and Illumina.
"3rd Generation" Sequencing includes "454" Pyrosequencing only.
"3rd Generation" Sequencing includes Illumina only.
"2nd Generation" Sequencing includes single molecule sequencing.
"3rd Generation" Sequencing includes single molecule sequencing.
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Cost by size of molecule being sequenced is lowering.
Speed of sequencing is rising.
Cost by size of molecule being sequenced is rising.
Speed of sequencing is lowering.
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It involves a combustion reaction at a molecular level.
Based on the release of "Pyrophosphate" (P-P) molecule.
Generates relatively long "reads"(~400bp)
Generates relatively short "reads"(~40bp)
Can sequence ~500Mbp DNA/day
Can sequence ~1000Mbp DNA/day
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"Synthetic" and used to study function of regulatory sequences (promoters, RNA stability, translation regulation, etc.).
"Natural" and used to study function of regulatory sequences (promoters, RNA stability, translation regulation, etc.).
Includes "regulatory sequence" from gene of interest.
Add a coding region for specific "Reporter Protein," one that is the same with an experiment.
Add a coding region for random "Reporter Protein," one that is the same with an experiment.
Add a coding region for specific "Reporter Protein," one that is different with an experiment.
Add a coding region for random "Reporter Protein," one that is different with an experiment.
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