Molecular Biology Quiz (Lectures 11 & 12)

14 Questions | By Jimmyd876 | Updated: Mar 22, 2022 | Attempts: 958

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1.

Southern Blots are used to study...
- A.
  
  DNA only.
- B.
  
  RNA only.
- C.
  
  Proteins only.
- D.
  
  DNA and RNA only.
- E.
  
  DNA and Proteins only.
- F.
  
  RNA and Proteins only.
- G.
  
  DNA, RNA, and Proteins.
2.

Which is the correct order for steps to be taken in a Southern Blot? I. Detect specific fragments by hybridizing DNA probe (radioactive or non-radioactive) to DNA on membrane. II. Denature dsDNA in gel using NaOH (high pH). III. Digest DNA with restriction enzyme. IV. Visualize hybridized bands with P-imager or film. V. Transfer ("blot") ssDNA from gel to membrane. VI. Size fractionate DNA fragments in agarose gel and stain with EtBr to visualize all DNA.
- A.
  
  III, VI, II, V, I, IV
- B.
  
  II, IV, V, VI, III, I
- C.
  
  IV, I, V, II, VI, III
- D.
  
  III, I, V, VI, II, IV
3.

What type of blot is used to detect a Restriction Fragment Length Polymorphism (RFLP), in order to analyze genetic variation?
4.

Northern Blots are used to study...
- A.
  
  DNA only.
- B.
  
  RNA only.
- C.
  
  Proteins only.
- D.
  
  DNA and RNA only.
- E.
  
  DNA and Proteins only.
- F.
  
  RNA and Proteins only.
- G.
  
  DNA, RNA, and Proteins.
5.

What are the major differences (besides the specific substance being studied) between northern and Southern blots?
- A.
  
  Northern Blots do not require restriction endonucleases, but the substance being studied must be denatured in heated formaldehyde, in stead of SDS.
- B.
  
  Southern Blots do not require restriction endonucleases, but the substance being studied must be denatured in heated formaldehyde, in stead of SDS.
- C.
  
  Northern Blots require restriction endonucleases, and the substance being studied must be denatured in heated formaldehyde, in stead of SDS.
- D.
  
  Southern Blots require restriction endonucleases, and the substance being studied must be denatured in heated formaldehyde, in stead of SDS.
6.

Sanger method of Sequencing DNA description: ________, _____ _____________.
7.

Cycle sequencing is...
- A.
  
  Is an improvement on Sanger method
- B.
  
  Allows automation.
- C.
  
  Includes "454" and "illumina" methods.
8.

Check all that are true with regards to Sanger Sequencing.
- A.
  
  Syntehsizes new strand of DNA in vitro.
- B.
  
  Includes dideoxy nucleotides (ddNTP) that terminate DNA synthesis.
- C.
  
  Each tube has known dideoxy nt (ddATP, ddGTP, ddTTP, or ddCTP).
9.

Differences between Automated Sequencing and Sanger Sequencing:
- A.
  
  DNA labeled with fluorescent dyes instead of (^35)S.
- B.
  
  Dyes are on Dideoxynucleotides(ddNTPs).
- C.
  
  DNA is electrophoresed in polyacrylamide gel.
- D.
  
  DNA labeled with (^35)S instead of fluorescent dyes.
- E.
  
  Dyes are on deoxynucleotides (dNTPs).
- F.
  
  DNA is electrophoresed in agarose gel.
- G.
  
  Detected by exciting dye with laser.
- H.
  
  Not detected by exciting dye with laser.
- I.
  
  Uses one color, and four lanes, one for each nucleotide, for detection.
- J.
  
  Uses one lane, and four colors, one for each nucleotide, for detection.
10.

Select those of the following that are true about "Next Generation" sequencing methods:
- A.
  
  "2nd Generation" Sequencing includes "454" Pyrosequencing and Illumina.
- B.
  
  "2nd Generation" Sequencing includes "454" Pyrosequencing only.
- C.
  
  "2nd Generation" Sequencing includes Illumina only.
- D.
  
  "3rd Generation" Sequencing includes "454" Pyrosequencing and Illumina.
- E.
  
  "3rd Generation" Sequencing includes "454" Pyrosequencing only.
- F.
  
  "3rd Generation" Sequencing includes Illumina only.
- G.
  
  "2nd Generation" Sequencing includes single molecule sequencing.
- H.
  
  "3rd Generation" Sequencing includes single molecule sequencing.
11.

The main benefit(s) of improvements on sequencing methods is(are)
- A.
  
  Cost by size of molecule being sequenced is lowering.
- B.
  
  Speed of sequencing is rising.
- C.
  
  Cost by size of molecule being sequenced is rising.
- D.
  
  Speed of sequencing is lowering.
12.

Which of the following are true about "454" Pyrosequencing?
- A.
  
  It involves a combustion reaction at a molecular level.
- B.
  
  Based on the release of "Pyrophosphate" (P-P) molecule.
- C.
  
  Generates relatively long "reads"(~400bp)
- D.
  
  Generates relatively short "reads"(~40bp)
- E.
  
  Can sequence ~500Mbp DNA/day
- F.
  
  Can sequence ~1000Mbp DNA/day
13.

Reporter Genes are:
- A.
  
  "Synthetic" and used to study function of regulatory sequences (promoters, RNA stability, translation regulation, etc.).
- B.
  
  "Natural" and used to study function of regulatory sequences (promoters, RNA stability, translation regulation, etc.).
- C.
  
  Includes "regulatory sequence" from gene of interest.
- D.
  
  Add a coding region for specific "Reporter Protein," one that is the same with an experiment.
- E.
  
  Add a coding region for random "Reporter Protein," one that is the same with an experiment.
- F.
  
  Add a coding region for specific "Reporter Protein," one that is different with an experiment.
- G.
  
  Add a coding region for random "Reporter Protein," one that is different with an experiment.