Microbial Diversity Methods Review Test

10 Questions | Total Attempts: 38

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Psychometrics Quizzes & Trivia

This quiz is designed to allow you to judge whether you have a basic knowledge of the methods to assess microbial diversity and community structure in soil.


Questions and Answers
  • 1. 
    All of these are examples of biochemical techniques that are used to assess microbial diversity and community structure in soil:
    • A. 

      DNA Microarrays

    • B. 

      PLFA

    • C. 

      Plating counts

    • D. 

      DGGE

    • E. 

      CLPP

  • 2. 
    This biochemical technique has been the traditional method to assess soil microbial diversity, but only a small proportion of microorganisms are determined (1%).
    • A. 

      Plating counts

    • B. 

      PLFA

    • C. 

      CLPP

    • D. 

      DGGE

    • E. 

      T-RFLP

  • 3. 
    These are some of the reasons why phospholipids are potentially useful signature molecules to assess microbial diversity:
    • A. 

      Important components of membranes of viable microorganisms

    • B. 

      Important in respiration process

    • C. 

      Phospholipid fatty acids are rapidly metabolized following cell death

    • D. 

      They are involved in nutrient mineralization and cycling

    • E. 

      They make up a fairly constant proportion of the biomass of organisms

  • 4. 
    This quantitative molecular method is useful when a coarse level of resolution is meaningful. Hint: it is not influenced by PCR biases
    • A. 

      CLPP

    • B. 

      DNA Microarrays

    • C. 

      DGGE

    • D. 

      C+G analysis

    • E. 

      T-RFLP

  • 5. 
    Molecular methods that are subject to PCR biases
    • A. 

      DGGE/TGGE

    • B. 

      T-RFLP

    • C. 

      RISA

    • D. 

      SSCP

    • E. 

      DNA Microarrays

  • 6. 
    This molecular technique relies on DNA polymorphisms and allows detection of only the labelled terminal restriction fragment.
    • A. 

      T-RFLP

    • B. 

      RISA

    • C. 

      DNA-Microarrays

    • D. 

      DGGE/TGGE

    • E. 

      G+C analysis

  • 7. 
    This molecular technique consists on  the amplification of  PCR  products of the intergenic spacer. 
    • A. 

      RISA

    • B. 

      C+G analysis

    • C. 

      T-RFLP

    • D. 

      DGGE/TGGE

    • E. 

      SSCP

  • 8. 
    This  method  relies  on   the ability of single-stranded DNA molecules to fold into unique secondary structures
    • A. 

      DNA Microarrays

    • B. 

      T-RFLP

    • C. 

      DGGE/TGGE

    • D. 

      SSCP

    • E. 

      RISA

  • 9. 
    This method consists on the separation of DNA clones, which is based on changes in the electrophoretic mobility of DNA fragments as they migrate through a gel containing a linearly increasing gradient of DNA denaturants.
    • A. 

      RISA

    • B. 

      SSCP

    • C. 

      T-RFLP

    • D. 

      DGGE/TGGE

    • E. 

      DNA Microarrays

  • 10. 
    This molecular technique allows analyzing thousands of genes. It is not influenced by PCR biases, but it  is only  accurate in low diversity systems
    • A. 

      SSCP

    • B. 

      DGGE/TGGE

    • C. 

      DNA Microarrays

    • D. 

      T-RFLP

    • E. 

      C+G analysis