Microbial Diversity Methods Review Test
This quiz is designed to allow you to judge whether you have a basic knowledge of the methods to assess microbial diversity and community structure in soil.
- 1.All of these are examples of biochemical techniques that are used to assess microbial diversity and community structure in soil:
- A.
DNA Microarrays
- B.
PLFA
- C.
Plating counts
- D.
DGGE
- E.
CLPP
- 2.This biochemical technique has been the traditional method to assess soil microbial diversity, but only a small proportion of microorganisms are determined (1%).
- A.
Plating counts
- B.
PLFA
- C.
CLPP
- D.
DGGE
- E.
T-RFLP
- 3.These are some of the reasons why phospholipids are potentially useful signature molecules to assess microbial diversity:
- A.
Important components of membranes of viable microorganisms
- B.
Important in respiration process
- C.
Phospholipid fatty acids are rapidly metabolized following cell death
- D.
They are involved in nutrient mineralization and cycling
- E.
They make up a fairly constant proportion of the biomass of organisms
- 4.This quantitative molecular method is useful when a coarse level of resolution is meaningful. Hint: it is not influenced by PCR biases
- A.
CLPP
- B.
DNA Microarrays
- C.
DGGE
- D.
C+G analysis
- E.
T-RFLP
- 5.Molecular methods that are subject to PCR biases
- A.
DGGE/TGGE
- B.
T-RFLP
- C.
RISA
- D.
SSCP
- E.
DNA Microarrays
- 6.This molecular technique relies on DNA polymorphisms and allows detection of only the labelled terminal restriction fragment.
- A.
T-RFLP
- B.
RISA
- C.
DNA-Microarrays
- D.
DGGE/TGGE
- E.
G+C analysis
- 7.This molecular technique consists on the amplification of PCR products of the intergenic spacer.
- A.
RISA
- B.
C+G analysis
- C.
T-RFLP
- D.
DGGE/TGGE
- E.
SSCP
- 8.This method relies on the ability of single-stranded DNA molecules to fold into unique secondary structures
- A.
DNA Microarrays
- B.
T-RFLP
- C.
DGGE/TGGE
- D.
SSCP
- E.
RISA
- 9.This method consists on the separation of DNA clones, which is based on changes in the electrophoretic mobility of DNA fragments as they migrate through a gel containing a linearly increasing gradient of DNA denaturants.
- A.
RISA
- B.
SSCP
- C.
T-RFLP
- D.
DGGE/TGGE
- E.
DNA Microarrays
- 10.This molecular technique allows analyzing thousands of genes. It is not influenced by PCR biases, but it is only accurate in low diversity systems
- A.
SSCP
- B.
DGGE/TGGE
- C.
DNA Microarrays
- D.
T-RFLP
- E.
C+G analysis
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