Microbiology Questions Quiz

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Microbiology Questions Quiz

The study of microbiology covers the area of science that deals with microscopic organisms, best defined as organisms so small that you typically cannot see them with the naked eye. Can you identify the different kinds of microorganisms out there – bacteria, fungi, protozoa, viruses – and tell us all about them and how they’re studied? Take the following quiz to find out just how much you know about them! Good luck!


Questions and Answers
  • 1. 
    Who proved that microorganisms cause disease?
    • A. 

      Pasteur

    • B. 

      Van Leeuwenhoek

    • C. 

      Koch

    • D. 

      Hooke

  • 2. 
    Nitrogen bases are building blocks of:
    • A. 

      Lipids

    • B. 

      Deoxyribose

    • C. 

      Nucleotides

    • D. 

      Ribosomes

    • E. 

      None of these

  • 3. 
    Proteins are formed by amino acids connected by:
    • A. 

      Alpha 1,4-glycosidic bond

    • B. 

      Phosphodiester linkage

    • C. 

      Hydrogen bonds

    • D. 

      Peptide bonds

  • 4. 
    Which of the following is not a kingdom in the five-kingdom system?
    • A. 

      Protista

    • B. 

      Algae

    • C. 

      Plant

    • D. 

      Animal

    • E. 

      Monera

  • 5. 
    The best use of a negative stain is:
    • A. 

      To determine cell shape

    • B. 

      To see endospore

    • C. 

      To determine Gram reaction

    • D. 

      To determine motility

  • 6. 
    • A. 

      Substrate level phosphorylation

    • B. 

      Oxidative level phosphorylation

  • 7. 
    Place the following steps in the correct sequence: 1 - Staining; 2- Making a smear; 3 - Fixing
    • A. 

      1-2-3

    • B. 

      1-3-2

    • C. 

      3-2-1

    • D. 

      2-3-1

  • 8. 
    Which of the following chemicals is found only in the outer membrane of Gram-negative bacteria?
    • A. 

      Plasmid

    • B. 

      Teichoic acid

    • C. 

      Flagella

    • D. 

      Lipopolysaccharide

    • E. 

      Peptidoglycan

  • 9. 
    • A. 

      The cell wall of G+ is much thicker than G-

    • B. 

      In Gram stain, G- are blue (purple) and G+ are pink (red)

    • C. 

      G+ and NOT G- contain teichoic acid in their cell wall

    • D. 

      In Gram stain, G+ are blue (purple) and G- are pink (red)

  • 10. 
    • A. 

      Gram positive cells have more peptidoglycan

    • B. 

      Gram positive cells have endotoxin

    • C. 

      Gram positive cells are sensitive to penicillin

    • D. 

      Gram positive cells are decolorized by alcohol-acetone

  • 11. 
    Which of the following statements are about a gram-negative cell wall is not true?
    • A. 

      It has teichoic acids

    • B. 

      It contains an endotoxin

    • C. 

      It protects the cell from osmotic lysis

    • D. 

      It maintains the shape of the cell

  • 12. 
    Which of the following is not a kingdom in the five-kingdom system?
    • A. 

      Animal

    • B. 

      Fungi

    • C. 

      Monera

    • D. 

      Plant

    • E. 

      Virus

  • 13. 
    Which of the following chemical bonds is the strongest?
    • A. 

      Ionic bond

    • B. 

      Covalent bond

    • C. 

      Hydrogen Bond

    • D. 

      They all have the same strength

  • 14. 
    Bacterial cell membrane DOES NOT contain:
    • A. 

      Phospholipids

    • B. 

      Proteins

    • C. 

      Glycoproteins

    • D. 

      Lipids

    • E. 

      Nucleic acids

  • 15. 
    All of the following can be found in mitochondria except:
    • A. 

      Circular DNA

    • B. 

      Ribosome

    • C. 

      ATP-generating mechanism

    • D. 

      Electron transport system

    • E. 

      Cell wall

  • 16. 
    This microscope uses an ultraviolet light source.
    • A. 

      Dark field microscope

    • B. 

      Fluorescence microscope

    • C. 

      Electron microscope

    • D. 

      Compound light microscope

  • 17. 
    These molecules are the primary building blocks of the plasma membrane in cells
    • A. 

      Carbohydrates

    • B. 

      Nucleic acids

    • C. 

      Proteins

    • D. 

      Lipids

  • 18. 
    Which of the following does not contribute to the hydrophobic interaction?
    • A. 

      Ionic interaction between hydrophobic molecules

    • B. 

      Proximity of hydrophobic molecules

    • C. 

      Increase entropy

    • D. 

      Exclusion of water

  • 19. 
    These are the primary molecules making up plasma membrane in cells
    • A. 

      Nucleic acids

    • B. 

      Carbohydrates

    • C. 

      Proteins

    • D. 

      Lipids

  • 20. 
    This is not a major bioelement
    • A. 

      Sulfur

    • B. 

      Nitrogen

    • C. 

      Hydrogen

    • D. 

      Magnesium

    • E. 

      Phosphate

  • 21. 
    A protein's function depends upon its unique conformation. Which level of protein folding achieves this conformation?
    • A. 

      Primary structure

    • B. 

      Beta sheet

    • C. 

      Alpha helix

    • D. 

      Secondary structure

    • E. 

      Tertiary structure

  • 22. 
    Which forces/bonds are involved in quaternary structure of proteins?
    • A. 

      Hydrogen bond, S-S bridges, Van der Waals forces, peptide bonds

    • B. 

      Hydrogen bond, S-S bridges, Van der Waals forces, peptide bonds, and hydrophobic interactions

    • C. 

      Hydrogen bond, S-S bridges, peptide bonds

    • D. 

      Hydrogen bond, S-S bridges, Van der Waals forces, hydrophobic interactions

  • 23. 
    This is not an internal structure of a bacteria
    • A. 

      Ribosome

    • B. 

      Plasmid

    • C. 

      Fimbriae

    • D. 

      Nucleoid

  • 24. 
    You can find nucleotides in
    • A. 

      DNA

    • B. 

      Amino acids

    • C. 

      Fatty acids

    • D. 

      Proteins

    • E. 

      Carbohydrates

  • 25. 
    Which are of microbiolgy studies fungi?
    • A. 

      Mycology

    • B. 

      Parasitology

    • C. 

      Biotechnology

    • D. 

      Immunology

    • E. 

      Bioremediation

  • 26. 
    Which of the following compounds has the greatest amount of energy for a cell?
    • A. 

      O2

    • B. 

      Glucose

    • C. 

      CO2

    • D. 

      ATP

    • E. 

      Pyruvate

  • 27. 
    Which of the following is not part of the cell membrane?
    • A. 

      Fatty acids

    • B. 

      Nucleic acids

    • C. 

      Sugars

    • D. 

      Amino acids

  • 28. 
    Cell division occurs in prokaryotes by:
    • A. 

      Budding

    • B. 

      Meiosis

    • C. 

      Binary Fission

    • D. 

      Mitosis

  • 29. 
    Which of the following mechanisms requires energy?
    • A. 

      Simple diffusion

    • B. 

      Osmosis

    • C. 

      Facilitated diffusion

    • D. 

      Active transport

  • 30. 
    Cells are made of mostly
    • A. 

      Lipids

    • B. 

      Proteins

    • C. 

      RNA

    • D. 

      DNA

    • E. 

      Carbohydrates

  • 31. 
    • A. 

      Pink

    • B. 

      Red

    • C. 

      Green

    • D. 

      Purple

    • E. 

      Orange

  • 32. 
    Eukarya
    • A. 

      Have a membrane around their DNA

    • B. 

      Contain outer membrane and cell membrane

    • C. 

      Consist of only multicellular organisms

    • D. 

      All of these are correct

    • E. 

      None of these are correct

  • 33. 
    The type of bond holding Na+ and Cl- ions in NaCl is a(n):
    • A. 

      Covalent bond

    • B. 

      Double bond

    • C. 

      Ionic bond

    • D. 

      Hydrogen bond

  • 34. 
    Loss of electrons means:
    • A. 

      Phosphorylation

    • B. 

      Reduction

    • C. 

      Oxidation

  • 35. 
    S-S agar inhibits gram-positive bacteria. On this medium, bacteria that ferment lactose to produce red colonies, and bacteria that do not ferment lactose to produce colorless colonies. This medium is:
    • A. 

      Both selective and differential

    • B. 

      Differential

    • C. 

      Enriched

    • D. 

      Selective

  • 36. 
    Which of the following is not a characteristic of cell membrane
    • A. 

      Selective permeability

    • B. 

      Composed of Phospholipid bilayer

    • C. 

      Contains proteins

    • D. 

      Maintain cell shape

    • E. 

      Present in all cells

  • 37. 
    Which of the following mechanisms generate ATP using an electron transport chain?
    • A. 

      Fermentation

    • B. 

      Glycolysis

    • C. 

      Oxidative phosphorylation

    • D. 

      Substrate level phosphorylation

    • E. 

      Krebs cycle

  • 38. 
    An example of bioremediation is the use of:
    • A. 

      Bacillus thuringiensis to kill crop-eating insects

    • B. 

      Cowpox virus to vaccinate against smallpox

    • C. 

      Saccharomyces cerevisie to make wine

    • D. 

      Pseudomonas to remove uranium from oil at weapons depots

  • 39. 
    This microscope achieves the highest magnification and greatest resolution.
    • A. 

      Flurorescence microscope

    • B. 

      Phase-contrast microscope

    • C. 

      Electron microscope

    • D. 

      Darkfield microscope

    • E. 

      Compound light microscope

  • 40. 
    All of the following organisms are eukaryotes except:
    • A. 

      Plants

    • B. 

      Archaea

    • C. 

      Protozoa

    • D. 

      Fungi

    • E. 

      All of these are eukaryotes

  • 41. 
    Which molecule is composed of a chain of amino acids?
    • A. 

      Carbohydrates

    • B. 

      Proteins

    • C. 

      Lipids

    • D. 

      Nucleic acids

  • 42. 
    The most common solidifying agent added to culture media is:
    • A. 

      Starch

    • B. 

      Silica gel

    • C. 

      Gelatin

    • D. 

      Agar

  • 43. 
    The best definition of biotechnology is:
    • A. 

      The use of living organisms to make desired products

    • B. 

      Curing diseases

    • C. 

      The use of microorganisms in sewage treatment

    • D. 

      The development of genetic engineering

  • 44. 
    Peptidoglycan is made of mostly:
    • A. 

      Proteins

    • B. 

      Lipids

    • C. 

      Nucleic acids

    • D. 

      Carbohydrates

  • 45. 
    Which of the following is not a product of TCA cycle?
    • A. 

      CO2

    • B. 

      Acetyle-CoA

    • C. 

      NADH

    • D. 

      GTP (ATP)

    • E. 

      FADH

  • 46. 
    Which of the following is a scientific name?
    • A. 

      Legionnaires' Disease

    • B. 

      Legionella Pneumophila

    • C. 

      Legionnares' bacterium

    • D. 

      Philadelphia fever

  • 47. 
    You have isolated a cell that lacks a membrane enclosed nucleus. You would find all of the following structures except  which one?
    • A. 

      A ribosome

    • B. 

      A chromosome

    • C. 

      Peptidoglycan

    • D. 

      A lysosome

  • 48. 
    This microscope is used to observe a specimen that emits light when illuminated with an ultra violet light and is an excellent diagnostic tool.
    • A. 

      Electron microscope

    • B. 

      Compound light microscope

    • C. 

      Fluorescence microscope

    • D. 

      Darkfield microscope

  • 49. 
    Which of the following statements about bacteria is not true?
    • A. 

      Bacteria are essential to the presence of life on Earth

    • B. 

      Everyone has bacteria in and on his body

    • C. 

      Bacteria are used to make some foods

    • D. 

      The only good bacterium is a dead bacterium

  • 50. 
    A denatured protein maintains its:
    • A. 

      Structural integrity (3D), but it loses its functions

    • B. 

      Tertiary structure

    • C. 

      Primary structure

    • D. 

      Quarternary structure

    • E. 

      Secondary structure

  • 51. 
    Cells containing the peptidoglycan layer are susceptible to penicillin. Which part of this molecule does this antibiotic affect?
    • A. 

      Formation of peptide bridge

    • B. 

      Synthesis of amino acids

    • C. 

      Synthesis of Muramic acid

    • D. 

      Synthesis of glucoseamine

  • 52. 
    The appearance of gram-negative bacteria after completing Gram stan is:
    • A. 

      Red

    • B. 

      Brown

    • C. 

      Purple

    • D. 

      Colorless

  • 53. 
    The idea of spontaneous generation postulated that:
    • A. 

      Living organisms could arise from non-living material

    • B. 

      Organisms could spontaneously evolve into more advance organisms

    • C. 

      Organisms could evolve into next generation of organisms

    • D. 

      Organisms could spontaneously arise from other living organisms

  • 54. 
    Assume you have bacterial culture that requires teichoic acid in its growth medium. If teichoic acid is not provided, the bacteria will not be able to make:
    • A. 

      Cell wall

    • B. 

      Capsule

    • C. 

      Cell membrane

    • D. 

      Enzymes

  • 55. 
    Cocci are:
    • A. 

      Round to oval bacteria

    • B. 

      Rod bacteria

    • C. 

      Cylindrical shape bacteria

    • D. 

      Bacillus type bacteria

    • E. 

      Spiral

  • 56. 
    Which of the following scientists and their discoveries is not correctly matched?
    • A. 

      Jenner-vaccination

    • B. 

      Lister-antibiotics

    • C. 

      Koch-germ theory of disease

    • D. 

      Ehrlich-chemotheraphy

  • 57. 
    A chemical bond formed by two atoms when they share pairs of electrons to fill their outer shells is a/an:
    • A. 

      Hydrophilic interaction

    • B. 

      Covalent bond

    • C. 

      Hydrogen bond

    • D. 

      Ionic bond

    • E. 

      Romantic bond

  • 58. 
    Which of the following organelles most closely resembles a prokaryotic cell?
    • A. 

      MItochondrion

    • B. 

      Cell wall

    • C. 

      Golgi complex

    • D. 

      Nucleus

    • E. 

      Vacuole

  • 59. 
    A molecule that contains both hydrophobic and hydrophilic groups is called:
    • A. 

      Amphipathic

    • B. 

      Fluid mosaic

    • C. 

      Isoelentric

    • D. 

      Amphoteric

    • E. 

      Isotonic

  • 60. 
    The most important environmental factor affecting growth is:
    • A. 

      Moisture

    • B. 

      PH

    • C. 

      Oxygen

    • D. 

      Temperature

  • 61. 
    • A. 

      Pasteur

    • B. 

      Koch

    • C. 

      Hooke

    • D. 

      Van Leeuwenhoek

  • 62. 
    At which growth temperture bacteria grow at the fastest rate?
    • A. 

      Maximum temperature

    • B. 

      Optimum temperature

    • C. 

      Minimum temperature

  • 63. 
    A peptide bond: (1) is a covalent bond (2) holds the primary structure of protein together (3) is between two hydrogen groups (4) is between carbon and nitrogen (5) is between two sugar molecules
    • A. 

      1 2 3 4

    • B. 

      1 2 3 4 5

    • C. 

      1 4 5

    • D. 

      2 3 4

    • E. 

      1 2 4

  • 64. 
    • A. 

      To keep bacteria under control

    • B. 

      To increase pH

    • C. 

      To decrease pH

    • D. 

      To separate microorganisms from one another

    • E. 

      To maintain pH at a certain level

  • 65. 
    The difference between simple diffusion and facilitated diffusion is that facilitated diffusion:
    • A. 

      Requires transporter proteins (carriers)

    • B. 

      Does not require ATP (energy)

    • C. 

      Requires ATP (energy)

    • D. 

      Can move materials from a higher to a lower concentration

    • E. 

      Can move materials from a lower to a higher concentration

  • 66. 
    By which of the following mechanisms can a cell transport a substance from a lower to a higher concentration?
    • A. 

      Simple diffusion

    • B. 

      Extracellular enzymes

    • C. 

      Facilitated diffusion

    • D. 

      Active transport

  • 67. 
    All of the following statements about cellulase are true except which one?
    • A. 

      Some bacteria make it

    • B. 

      It is a carbohydrate

    • C. 

      It is used to degrade cellulose

    • D. 

      It is a protein

  • 68. 
    Which of the following statements is true?
    • A. 

      Endospores are for reproduction

    • B. 

      A cell can produce many endospores

    • C. 

      Endospores allow cell to survive environmental changes

    • D. 

      A cell produces one endospore and keeps growing

    • E. 

      Endospores are easily stained in Gram stain

  • 69. 
    All of the following are true about starch and cellulose except they are:
    • A. 

      Polymers of glucose

    • B. 

      Digested by animals

    • C. 

      Digested by many bacteria and fungi

    • D. 

      Produced by plants

  • 70. 
    A medium having higher concentration of solutes than interior of the cell is a ______ ______, which causes cell to _________
    • A. 

      Hypertonic solution, shrivel

    • B. 

      Isotonic solution, swell

    • C. 

      Hypertonic solution, swell

    • D. 

      Hypotonic solution, shrivel

    • E. 

      Hypotonic solution, swell

  • 71. 
    The fluid mosaic model describes
    • A. 

      Cytoplasm

    • B. 

      Cell membrane

    • C. 

      Cell wall

    • D. 

      Gram negative outer membrane

  • 72. 
    Which of the following is not related to bacterial cell wall
    • A. 

      Made of peptidoglycan

    • B. 

      Controls water balance

    • C. 

      Not all bacteria have cell wall

    • D. 

      Preserve shape of bacteria

    • E. 

      All of these are related to bacterial cell wall

  • 73. 
    Name the two macromolecules that are considered essence of life
    • A. 

      Proteins and lipids

    • B. 

      Carbohydrates and lipids

    • C. 

      Carbohydrates and proteins

    • D. 

      Nucleic acids and proteins

    • E. 

      Nucleic acids and lipids

  • 74. 
    Bacterial cell wall is made of:
    • A. 

      Cellulose

    • B. 

      Lipopolysaccharides

    • C. 

      Murein

    • D. 

      Phospholipids

    • E. 

      Proteins

  • 75. 
    Bioremediation refers to:
    • A. 

      Using microbe to study diseases

    • B. 

      Using microbe in vaccine development

    • C. 

      Using microbes to clean up pollution

    • D. 

      Rehabilitating wayward bacteria

  • 76. 
    Eukaryotes include all BUT
    • A. 

      Algae

    • B. 

      Fungi

    • C. 

      Plants

    • D. 

      Animals

    • E. 

      Bacteria

  • 77. 
    In the name of escherichia coli, coli is the:
    • A. 

      Specific epithet family

    • B. 

      Genus

    • C. 

      Species

    • D. 

      Kingdom

  • 78. 
    • A. 

      b and d

    • B. 

      B and c

    • C. 

      A and c

    • D. 

      C and d

    • E. 

      A and b

  • 79. 
    All of the following statements are true about the plasma membrane except which?
    • A. 

      The lipids and proteins move across the membrane

    • B. 

      Small ions can easily cross the membrane

    • C. 

      Integral proteins make channels in the membrane

    • D. 

      Peripheral proteins catalyze chemical reactions

  • 80. 
    Which of the following pairs are mismatched?
    • A. 

      Ehrlich - chemoterarphy

    • B. 

      Jenner - vaccination

    • C. 

      Pasteur - proof of biogenesis

    • D. 

      Koch - aseptic surgery

  • 81. 
    Robert koch established his postulates using the disease
    • A. 

      Gonorrhea

    • B. 

      Tubeculosis

    • C. 

      Diptheria

    • D. 

      Anthrax

    • E. 

      Syphilis

  • 82. 
    Which one makes the most ATP; aerobic respiration, anaerobic respiration or fermentation?
    • A. 

      Anaerobic respiration

    • B. 

      Fermentation

    • C. 

      Aerobic respiration

    • D. 

      They all basically make the same number of ATP

  • 83. 
    Which of the following pairs is mismatched?
    • A. 

      Leeuwenhoek - microscope

    • B. 

      Pasteur - proof of biogenesis

    • C. 

      Jenner - vaccination

    • D. 

      Koch - aseptic surgery