Micro Practice Practical For Perryman Lab

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    MSA stands for

    • Mannitol Salt Algar
    • Media Sulfur Acid
    • My Sweet Alex
    • MultiSellular Actins
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Micro Practice Practical For Perryman Lab - Quiz

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  • 2. 

    Staphylococcus aureus is the only Staph that has

    • Teticohalogen

    • Coagulase

    • Leukocidin

    • Staphylokinase

    Correct Answer
    A. Coagulase
    Explanation
    Coagulase is an enzyme produced by Staphylococcus aureus that is responsible for the formation of fibrin clots. This ability is unique to Staphylococcus aureus and is not found in other staphylococcal species. Coagulase plays a crucial role in the pathogenesis of Staphylococcus aureus infections by helping the bacteria evade the host immune response and form protective barriers.

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  • 3. 

    UreaseTest has a pH indicator of Phenol Red

    • True

    • False

    Correct Answer
    A. True
    Explanation
    The Urease Test is a biochemical test used to determine the ability of an organism to produce the enzyme urease. Urease hydrolyzes urea to produce ammonia and carbon dioxide. In this test, a pH indicator called Phenol Red is used. Phenol Red is red at a neutral pH (around 7), but turns yellow at an acidic pH (below 6.8) and pink at a basic pH (above 8.2). Since the Urease Test is designed to detect the production of ammonia, which is basic, a positive result (indicating the presence of urease) is indicated by a color change from red to pink, confirming that the pH has become more basic. Therefore, the statement that Urease Test has a pH indicator of Phenol Red is true.

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  • 4. 

    Test: Urease Test Results: Media turns Hot pink Interpretation:____________

    • Negative for Urease

    • Positive for Urease

    • Organism not properly innoculated

    • None of the others

    Correct Answer
    A. Positive for Urease
    Explanation
    The interpretation for the given test result is that the organism tested is positive for urease. Urease is an enzyme produced by certain bacteria that breaks down urea into ammonia and carbon dioxide. A positive result in the urease test indicates that the organism is capable of producing urease and can hydrolyze urea. This is supported by the media turning hot pink, which is a characteristic color change associated with a positive urease reaction.

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  • 5. 

    Catalase Test causes what reaction in Staphs?

    • Bubbling

    • Smoking

    • Stringing

    • Color Change

    • None of the others

    Correct Answer
    A. Bubbling
    Explanation
    The catalase test causes bubbling in Staphs. This reaction occurs because catalase is an enzyme that breaks down hydrogen peroxide into water and oxygen. When hydrogen peroxide is added to a sample containing Staphylococci, the catalase enzyme present in the bacteria catalyzes the breakdown of hydrogen peroxide, resulting in the release of oxygen gas. This release of oxygen gas causes bubbling to occur, indicating a positive catalase test.

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  • 6. 

    Test: Hymolysis Result: Colonies grow well but no change in medium. Interpretation: ____________________________

    • Beta

    • Gamma

    • Alpha

    • Delta

    • Gram negative

    • Gram positive

    • Improper inoculation/ contaminated results

    Correct Answer
    A. Gamma
    Explanation
    The interpretation for the given result is that the colonies grow well but there is no change in the medium. This indicates that the test result is gamma.

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  • 7. 

    Triple Sugar Iron Test Read the results(check all that apply): Yellow Slant & Yellow Butt 
    • A- Acid
    • K-Alkaline
    • - - Nuetral

    • A/A

    • K/A

    • K/K

    • K/-

    • -/-

    • H2S

    • CO2

    Correct Answer
    A. A/A
    Explanation
    The given results indicate that both the slant and the butt of the Triple Sugar Iron (TSI) test turned yellow, which corresponds to an acidic environment. Therefore, the correct answer is A/A, indicating an acid production in both glucose and lactose/sucrose fermentation.

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  • 8. 

    Triple Sugar Iron Test Read the results(check all that apply): Pink Slant & Yellow Butt 
    • A- Acid
    • K-Alkaline
    • - - Nuetral

    • A/A

    • K/A

    • K/K

    • K/-

    • -/-

    • H2S

    • CO2

    Correct Answer
    A. K/A
    Explanation
    The result "K/A" indicates that the Pink Slant is alkaline (K) and the Yellow Butt is acidic (A). This result suggests that glucose fermentation has occurred, producing acid in the butt of the tube, while lactose and/or sucrose fermentation has also occurred, producing alkali in the slant of the tube.

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  • 9. 

    Collagenase can be detected by use of Gelatin agar.

    • True

    • False

    Correct Answer
    A. True
    Explanation
    Page 164

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  • 10. 

    Catalase is a fast test to determine whether an organism is a Staph or Strep

    • True

    • False

    Correct Answer
    A. True
    Explanation
    Catalase is indeed a fast test that can be used to differentiate between Staphylococcus and Streptococcus organisms. This test involves adding hydrogen peroxide to a bacterial culture, and if the bacteria produce the enzyme catalase, they will break down the hydrogen peroxide into water and oxygen, resulting in the formation of bubbles. Staphylococcus species produce catalase, while Streptococcus species do not. Therefore, the statement that catalase is a fast test to determine whether an organism is a Staph or Strep is true.

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  • 11. 

    What enzyme hydrolyzes the casein protein?

    • Amylase

    • DNase

    • Collagenase

    • Caseinase

    • Lipase

    Correct Answer
    A. Caseinase
    Explanation
    Page 161

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  • 12. 

    What enzyme hydrolyzes the substrate deoxyribonucleic acid?

    • Amylase

    • DNase

    • Collagenase

    • Caseinase

    • Lipase

    Correct Answer
    A. DNase
    Explanation
    Page 161

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  • 13. 

    What enzyme hydrolyzes the substrate starch?

    • Amylase

    • DNase

    • Collagenase

    • Caseinase

    • Lipase

    Correct Answer
    A. Amylase
    Explanation
    Page 161

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  • 14. 

    What enzyme degrades hydrogen peroxide to water and oxygen gas?

    Correct Answer
    Catalase
    catalase
    catlase
    Catlase
    Catalace
    Catalace
    Explanation
    Catalase is the correct answer for this question. Catalase is an enzyme that is found in cells and helps to break down hydrogen peroxide into water and oxygen gas. It plays a crucial role in protecting cells from the harmful effects of hydrogen peroxide, which is a byproduct of various metabolic processes. By catalyzing the decomposition of hydrogen peroxide, catalase ensures that it is converted into harmless substances, preventing any damage to the cell.

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  • 15. 

    If an organism has growth on a EMB plate it is

    • Gram Positive

    • Gram Negative

    • Staphylococcus

    • Streptococcus

    Correct Answer
    A. Gram Negative
    Explanation
    p 174

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  • 16. 

    MSA identifies mmembers of

    • Streptococcus

    • Staphylococcus

    • Bacillus

    • Gram Positive

    Correct Answer
    A. Staphylococcus
    Explanation
    MSA (Mannitol Salt Agar) is a selective and differential medium used to identify and isolate Staphylococcus species. It contains high salt concentration, which inhibits the growth of most bacteria except for Staphylococcus, which can tolerate high salt levels. The medium also contains mannitol sugar, which is fermented by certain Staphylococcus species, causing a change in the pH of the agar and resulting in a yellow color change. Therefore, the presence of yellow colonies on MSA indicates the presence of Staphylococcus.

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  • 17. 

    Test: Hymolysis Result: Colonies have a clear zone around them. Plate is see through when held up to light. Interpretation: ____________________________

    • Beta

    • Gamma

    • Alpha

    • Delta

    • Gram negative

    • Gram positive

    • Improper inoculation/ contaminated results

    Correct Answer
    A. Beta
    Explanation
    The interpretation for the given test result is that the colonies exhibit beta hemolysis. This is indicated by the presence of a clear zone around the colonies on the plate, which becomes see-through when held up to light.

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  • 18. 

    If an organism has growth on a PEA plate it is

    • Gram Positive

    • Gram Negative

    • Staphylococcus

    • Streptococcus

    Correct Answer
    A. Gram Positive
    Explanation
    p 174

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  • 19. 

    After adding Gram's Iodine starch agar turns purple unless there is a presence of this enzyme

    • Amylase

    • DNase

    • Collagenase

    • Caseinase

    • Lipase

    Correct Answer
    A. Amylase
    Explanation
    Page 163

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  • 20. 

    Media: EMB Plate Results: Organism has growth. Which of the following can you interpreate from the results about the organism?

    • The organism is Gram Negative

    • The organism is Gram Positive

    • The organism is Gram Negative and Ferments Lactose

    • The organism is E.Coli

    • The Organism is Gram Positive and Ferments Lactose

    • None of the others

    Correct Answer
    A. The organism is Gram Negative
    Explanation
    P 174

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  • 21. 

    MSA has a pH indicator, if the agar turns yellow, the results should be interpreted as

    • Positive for Mannitol

    • Negative for Mannitol

    • Positive for NaCl

    • Negative for NaCl

    Correct Answer
    A. Positive for Mannitol
    Explanation
    If the MSA agar turns yellow, it indicates a low pH level. This change in color suggests that the bacteria present on the agar have fermented the mannitol sugar, producing acid as a byproduct. Therefore, the result is interpreted as positive for mannitol fermentation.

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  • 22. 

    Hemolysis is

    • Lysis of red blood cells.

    • Lysis of whit blood cells.

    • Lysis of muscle cells.

    • Lysis of delta cells.

    Correct Answer
    A. Lysis of red blood cells.
    Explanation
    Hemolysis refers to the breakdown or destruction of red blood cells. This process can occur due to various factors such as infections, toxins, or certain medical conditions. When red blood cells undergo hemolysis, their membranes rupture, leading to the release of their contents into the surrounding fluid. This can result in the release of hemoglobin, which gives the characteristic red color to blood, and other cellular components. Hemolysis can have detrimental effects on the body, including anemia and the release of substances that can cause inflammation and damage to tissues.

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  • 23. 

    Startch Hydrolysis can be tested with

    • The IMViC series.

    • A starch agar plate.

    • A DNA agar plate.

    • A Gram Stain.

    • A tube of gelatin agar, and Gram's iodine.

    Correct Answer
    A. A starch agar plate.
    Explanation
    Page 162

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  • 24. 

    EMB is selective for what Gram___________ Organisms

    • Positive

    • Negative

    Correct Answer
    A. Negative
    Explanation
    p. 172

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  • 25. 

    Which media is not only selective but differental as well?

    • PEA

    • EMB

    • PIE

    • ERA

    Correct Answer
    A. EMB
    Explanation
    EMB has lactose which detects lactose fermentation in EMB media.

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  • 26. 

    Hymolysis test is done on what type of agar?

    • DNA

    • Starch

    • Blood

    • Glucose

    Correct Answer
    A. Blood
    Explanation
    The correct answer is blood because the hemolysis test is performed on blood agar. This test is used to determine the ability of bacteria to break down red blood cells. Different types of hemolysis patterns can be observed on the blood agar, including alpha, beta, and gamma hemolysis, which can help in identifying different bacterial species.

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  • 27. 

    Test: Hymolysis Result: Colonies have a brownish green zone when held up to light. Interpretation: ____________________________

    • Beta

    • Gamma

    • Alpha

    • Delta

    • Gram negative

    • Gram positive

    • Improper inoculation/ contaminated results

    Correct Answer
    A. Alpha
    Explanation
    The interpretation for the given result is that the colonies exhibit a brownish green zone when held up to light, which indicates an Alpha hemolysis.

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  • 28. 

    PEA is selective for what Gram___________ Organisms

    • Positive

    • Negative

    Correct Answer
    A. Positive
    Explanation
    p. 172

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  • 29. 

    Media: MSA Plate Results: Growth with Yellowing of the Medium Which of the following can you interpreate from the results about the organism?

    • Staphylococcus

    • Streptococcus

    • Staphylococcus Ferments Mannitol

    • Streptococcus Ferments Mannitol

    • None of the others

    Correct Answer
    A. Staphylococcus Ferments Mannitol
    Explanation
    P 180

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  • 30. 

    In Gelatin Hydrolosis if the organism and media become liquid this means that the organism is positive for Collagenase.

    • True

    • False

    Correct Answer
    A. True
    Explanation
    Page. 164-165

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  • 31. 

    How Does MSA Work? What makes it Differential?

    • 7.5% NaCl

    • Ph balance of 6.8

    • PH indicator Phenol Red

    • Mannitol

    Correct Answer
    A. Mannitol
    Explanation
    Mannitol is the component that makes MSA (Mannitol Salt Agar) differential. MSA is a selective and differential medium used for the isolation and differentiation of Staphylococcus aureus from other staphylococci. The high concentration of salt (7.5% NaCl) in MSA inhibits the growth of most bacteria except for staphylococci. Staphylococcus aureus, which can ferment mannitol, produces acid that lowers the pH of the medium, causing a color change in the pH indicator phenol red from red to yellow. Other staphylococci that cannot ferment mannitol do not produce acid and the medium remains red. Therefore, the ability to ferment mannitol differentiates S. aureus from other staphylococci.

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  • 32. 

    Phenol Red in the Urease Test is turned what color by ammonia?

    • Hot Pink

    • Yellow

    • Clear

    • Methyl Red

    • Metallic Green

    Correct Answer
    A. Hot Pink
    Explanation
    Phenol Red in the Urease Test is turned hot pink by ammonia. This is because ammonia is alkaline and causes a change in the pH of the solution, causing the phenol red indicator to shift from its original color to hot pink.

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  • 33. 

    What enzyme hydrolyzes the breakdown of tributyrin?

    • Amylase

    • DNase

    • Collagenase

    • Caseinase

    • Lipase

    Correct Answer
    A. Lipase
    Explanation
    Page 161

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  • 34. 

    If you are innoculating as organism that has a presence of DNase in DNA agar, the agar will turn what color?

    • Off white

    • Pale Green

    • Methyl Green

    • Metallic Green

    • Clear

    Correct Answer
    A. Clear
    Explanation
    Page 164

    If DNase is present than the DNA will be broken down leaving the agar colorless.

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  • 35. 

    EMB containes

    • Phenylethyl alcohol

    • Phenol red

    • Potassium eosin acids

    • Eosin and methylene blue

    Correct Answer
    A. Eosin and methylene blue
    Explanation
    p. 172

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  • 36. 

    Catalase Test causes what reaction in Streps?

    • Bubbling

    • Smoking

    • Stringing

    • Color Change

    • None of the others

    Correct Answer
    A. None of the others
    Explanation
    Catalase has No reaction for Streps

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  • 37. 

    Test: Urease Test Results: Media turns Yellow Interpretation:____________

    • Negative for Urease

    • Positive for Urease

    • Organism not properly innoculated

    • None of the others

    Correct Answer
    A. Negative for Urease
    Explanation
    The interpretation of the result "Media turns Yellow" in the Urease Test is that the test is negative for Urease. This means that the organism being tested does not produce the enzyme urease, which is responsible for breaking down urea into ammonia and carbon dioxide. If the test were positive for Urease, the media would turn pink or magenta due to the presence of ammonia. Therefore, the correct answer is Negative for Urease.

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  • 38. 

    DNA Hydrolysis can be tested with

    • The IMViC series.

    • A starch agar plate.

    • A DNA agar plate.

    • A Gram Stain.

    • A tube of gelatin agar, and Gram's iodine.

    Correct Answer
    A. A DNA agar plate.
    Explanation
    Page 163

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  • 39. 

    PEA containes

    • Phenylethyl alcohol

    • Phenol red

    • Potassium eosin acids

    • Eosin and methylene blue

    Correct Answer
    A. Phenylethyl alcohol
    Explanation
    p. 172

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  • 40. 

    Which is Halophilic

    • Streptococcus

    • Staphylococcus

    Correct Answer
    A. Staphylococcus
    Explanation
    Staphylococcus is halophilic because it is able to tolerate high salt concentrations. This bacterium can survive and thrive in environments with high salt content, such as salty foods or the human skin. Streptococcus, on the other hand, is not halophilic and does not have the ability to tolerate high salt concentrations.

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  • 41. 

    Streps have what distint classes?

    • Alpha Beta Gamma

    • Alpha Beta Delta

    • Enterococcus and Pneumococcus

    • Gram negative and Gram positive

    Correct Answer
    A. Alpha Beta Gamma
    Explanation
    The correct answer is Alpha Beta Gamma. This suggests that streps have three distinct classes: Alpha, Beta, and Gamma.

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  • 42. 

    After Innoculation, Serratia marcescens...

    • Has a red pigment.

    • Has a metallic green sheen color.

    • Resembles a black caterpillar.

    • Must have phenol red dye added to it to see it.

    Correct Answer
    A. Has a red pigment.
    Explanation
    Page. 164
    fig. 19.3

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  • 43. 

    Media: PEA Plate Results: Organism has no growth. Which of the following can you interpreate from the results about the organism?

    • The organism is Gram Negative

    • The organism is Gram Positive

    • The organism is Gram Negative and Ferments Lactose

    • The organism is E.Coli

    • The Organism is Gram Positive and Ferments Lactose

    • None of the others

    Correct Answer
    A. The organism is Gram Negative
    Explanation
    P 174

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  • 44. 

    MSA is 

    • Selective Media

    • Differential Media

    • Selective and Differential Media

    • None of the others

    Correct Answer
    A. Selective and Differential Media
    Explanation
    Selective and differential media (SD media) is a type of culture medium that is designed to selectively promote the growth of certain microorganisms while inhibiting the growth of others. It contains specific nutrients or chemicals that favor the growth of particular organisms, allowing them to grow while suppressing the growth of other organisms. Additionally, SD media also contains indicators that can differentiate between different types of microorganisms based on their metabolic activities or biochemical characteristics. Therefore, MSA (Mannitol Salt Agar) can be classified as both selective and differential media as it selects for the growth of salt-tolerant bacteria and differentiates between bacteria based on their ability to ferment mannitol.

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  • 45. 

    Gram's Iodine is added to media after incubation to determine the presence of DNase. 

    • True

    • False

    Correct Answer
    A. False
    Explanation
    Page 162
    Gram's Iodine is added to media after incubation to determine the presence of Amylace.

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  • 46. 

    Triple Sugar Iron Test Read the results(check all that apply): Yellow Slant & Black Butt  
    • A- Acid
    • K-Alkaline
    • - - Nuetral

    • A/A

    • K/A

    • K/K

    • K/-

    • -/-

    • H2S

    • CO2

    Correct Answer(s)
    A. A/A
    A. H2S
    Explanation
    The given correct answer is A/A, H2S. This indicates that the test result shows a yellow slant and a black butt, which signifies an acid reaction. Additionally, the presence of H2S (hydrogen sulfide) is indicated.

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  • 47. 
    With Urease and Water becomes what? - ProProfs

    With Urease and Water becomes what?

    • 2NH3+CO2

    • 3NH2+NaCl

    • NaCl+CO2

    • 3NH2+O

    Correct Answer
    A. 2NH3+CO2
    Explanation
    When Urease reacts with Water, it produces 2 molecules of Ammonia (NH3) and 1 molecule of Carbon Dioxide (CO2). Therefore, the correct answer is 2NH3+CO2.

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  • 48. 

    What enzyme hydrolyzes the substrate gelatin?

    • Amylase

    • DNase

    • Collagenase

    • Caseinase

    • Lipase

    Correct Answer
    A. Collagenase
    Explanation
    Page 161

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  • 49. 

    After Innoculating in Starch agar, and adding Gram's Iodine, if the agar is clear around the organism, the results are?

    • Positive

    • Negative

    • Contaminated

    • False Positive

    Correct Answer
    A. Positive
    Explanation
    Page 162

    If Amylase is present than the Starch will be broken down leaving the agar colorless.

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    Quiz Edited by
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    Quiz Created by
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