Micro Practice Practical For Perryman Lab

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    Many Bacteria and Fungi Produce what that they release into their enviroment?

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Feel free to take this mock exam as many times as you would like. *Remember this Exam is for fun and to review your knowledge. It will in no way effect your final grade, and should not be used as a substitute for studying. *

Micro Practice Practical For Perryman Lab - Quiz

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  • 2. 

    What enzyme hydrolyzes the substrate starch?

    • Amylase

    • DNase

    • Collagenase

    • Caseinase

    • Lipase

    Correct Answer
    A. Amylase
    Explanation
    Page 161

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  • 3. 

    What enzyme hydrolyzes the substrate gelatin?

    • Amylase

    • DNase

    • Collagenase

    • Caseinase

    • Lipase

    Correct Answer
    A. Collagenase
    Explanation
    Page 161

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  • 4. 

    What enzyme hydrolyzes the casein protein?

    • Amylase

    • DNase

    • Collagenase

    • Caseinase

    • Lipase

    Correct Answer
    A. Caseinase
    Explanation
    Page 161

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  • 5. 

    What enzyme hydrolyzes the breakdown of tributyrin?

    • Amylase

    • DNase

    • Collagenase

    • Caseinase

    • Lipase

    Correct Answer
    A. Lipase
    Explanation
    Page 161

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  • 6. 

    Startch Hydrolysis can be tested with

    • The IMViC series.

    • A starch agar plate.

    • A DNA agar plate.

    • A Gram Stain.

    • A tube of gelatin agar, and Gram's iodine.

    Correct Answer
    A. A starch agar plate.
    Explanation
    Page 162

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  • 7. 

    Gram's Iodine is added to media after incubation to determine the presence of DNase. 

    • True

    • False

    Correct Answer
    A. False
    Explanation
    Page 162
    Gram's Iodine is added to media after incubation to determine the presence of Amylace.

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  • 8. 

    DNA Hydrolysis can be tested with

    • The IMViC series.

    • A starch agar plate.

    • A DNA agar plate.

    • A Gram Stain.

    • A tube of gelatin agar, and Gram's iodine.

    Correct Answer
    A. A DNA agar plate.
    Explanation
    Page 163

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  • 9. 

    After adding Gram's Iodine starch agar turns purple unless there is a presence of this enzyme

    • Amylase

    • DNase

    • Collagenase

    • Caseinase

    • Lipase

    Correct Answer
    A. Amylase
    Explanation
    Page 163

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  • 10. 

    After innoculating an organism in Starch agar and adding Gram's Iodine, if the organism has a presence of amylase the starch agar will turn what color?

    • Purple

    • Red

    • Yellow

    • Metallic Green

    • None of the others

    Correct Answer
    A. None of the others
    Explanation
    Page 163

    If amylase is present than the starch will be broken down leaving the agar colorless.

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  • 11. 

    If you are innoculating as organism that has a presence of DNase in DNA agar, the agar will turn what color?

    • Off white

    • Pale Green

    • Methyl Green

    • Metallic Green

    • Clear

    Correct Answer
    A. Clear
    Explanation
    Page 164

    If DNase is present than the DNA will be broken down leaving the agar colorless.

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  • 12. 

    After Innoculating in DNA agar, if the agar is methly green around the organism, the results are?

    • Positive

    • Negative

    • Contaminated

    • False Positive

    Correct Answer
    A. Negative
    Explanation
    Page 164

    If DNase is present than the DNA will be broken down leaving the agar colorless.

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  • 13. 

    After Innoculating in Starch agar, and adding Gram's Iodine, if the agar is clear around the organism, the results are?

    • Positive

    • Negative

    • Contaminated

    • False Positive

    Correct Answer
    A. Positive
    Explanation
    Page 162

    If Amylase is present than the Starch will be broken down leaving the agar colorless.

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  • 14. 

    Starch agars is a Selective Media.

    • True

    • False

    Correct Answer
    A. False
    Explanation
    Page 162

    Starch agar detects the presence of Amylace but any organism will be able to grow on Starch agar, meaning that it is NOT Selective.

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  • 15. 

    In Gelatin Hydrolosis if the organism and media become liquid this means that the organism is positive for Collagenase.

    • True

    • False

    Correct Answer
    A. True
    Explanation
    Page. 164-165

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  • 16. 

    After Innoculation, Serratia marcescens...

    • Has a red pigment.

    • Has a metallic green sheen color.

    • Resembles a black caterpillar.

    • Must have phenol red dye added to it to see it.

    Correct Answer
    A. Has a red pigment.
    Explanation
    Page. 164
    fig. 19.3

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  • 17. 

    Collagenase can be detected by use of Gelatin agar.

    • True

    • False

    Correct Answer
    A. True
    Explanation
    Page 164

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  • 18. 

    Can any organism grow in differential media?

    • Yes

    • No

    Correct Answer
    A. Yes
    Explanation
    Differential media will allow all organisms to grow.

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  • 19. 

    PEA is selective for what Gram___________ Organisms

    • Positive

    • Negative

    Correct Answer
    A. Positive
    Explanation
    p. 172

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  • 20. 

    EMB is selective for what Gram___________ Organisms

    • Positive

    • Negative

    Correct Answer
    A. Negative
    Explanation
    p. 172

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  • 21. 

    PEA containes

    • Phenylethyl alcohol

    • Phenol red

    • Potassium eosin acids

    • Eosin and methylene blue

    Correct Answer
    A. Phenylethyl alcohol
    Explanation
    p. 172

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  • 22. 

    EMB containes

    • Phenylethyl alcohol

    • Phenol red

    • Potassium eosin acids

    • Eosin and methylene blue

    Correct Answer
    A. Eosin and methylene blue
    Explanation
    p. 172

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  • 23. 

    Which media is not only selective but differental as well?

    • PEA

    • EMB

    • PIE

    • ERA

    Correct Answer
    A. EMB
    Explanation
    EMB has lactose which detects lactose fermentation in EMB media.

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  • 24. 

    If an organism has growth on a PEA plate it is

    • Gram Positive

    • Gram Negative

    • Staphylococcus

    • Streptococcus

    Correct Answer
    A. Gram Positive
    Explanation
    p 174

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  • 25. 

    If an organism has growth on a EMB plate it is

    • Gram Positive

    • Gram Negative

    • Staphylococcus

    • Streptococcus

    Correct Answer
    A. Gram Negative
    Explanation
    p 174

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  • 26. 

    Media: EMB Plate Results: Organism has no growth. Which of the following can you interpreate from the results about the organism?

    • The organism is Gram Negative

    • The organism is Gram Positive

    • The organism is Gram Negative and Ferments Lactose

    • The organism is E.Coli

    • The Organism is Gram Positive and Ferments Lactose

    • None of the others

    Correct Answer
    A. The organism is Gram Positive
    Explanation
    P 174

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  • 27. 

    Media: PEA Plate Results: Organism has no growth. Which of the following can you interpreate from the results about the organism?

    • The organism is Gram Negative

    • The organism is Gram Positive

    • The organism is Gram Negative and Ferments Lactose

    • The organism is E.Coli

    • The Organism is Gram Positive and Ferments Lactose

    • None of the others

    Correct Answer
    A. The organism is Gram Negative
    Explanation
    P 174

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  • 28. 

    Media: PEA Plate Results: Organism has growth. Which of the following can you interpreate from the results about the organism?

    • The organism is Gram Negative

    • The organism is Gram Positive

    • The organism is Gram Negative and Ferments Lactose

    • The organism is E.Coli

    • The Organism is Gram Positive and Ferments Lactose

    • None of the others

    Correct Answer
    A. The organism is Gram Positive
    Explanation
    P 174

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  • 29. 

    Media: EMB Plate Results: Organism has growth. Which of the following can you interpreate from the results about the organism?

    • The organism is Gram Negative

    • The organism is Gram Positive

    • The organism is Gram Negative and Ferments Lactose

    • The organism is E.Coli

    • The Organism is Gram Positive and Ferments Lactose

    • None of the others

    Correct Answer
    A. The organism is Gram Negative
    Explanation
    P 174

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  • 30. 

    Media: EMB Plate Results: Organism has Metalic Green Sheen. Which of the following can you interpreate from the results about the organism?

    • The organism is Gram Negative

    • The organism is Gram Positive

    • The organism is Gram Negative and Ferments Lactose

    • The organism is E.Coli

    • The Organism is Gram Positive and Ferments Lactose

    • None of the others

    Correct Answer
    A. The organism is E.Coli
    Explanation
    P 174

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  • 31. 

    Media: EMB Plate Results: Organism has growth with dark circles. Which of the following can you interpreate from the results about the organism?

    • The organism is Gram Negative

    • The organism is Gram Positive

    • The organism is Gram Negative and Ferments Lactose

    • The organism is E.Coli

    • The Organism is Gram Positive and Ferments Lactose

    • None of the others

    Correct Answer
    A. The organism is Gram Negative and Ferments Lactose
    Explanation
    P 174

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  • 32. 

    Media: MSA Plate Results: No Growth Which of the following can you interpreate from the results about the organism?

    • Staphylococcus

    • Streptococcus

    • Staphylococcus Ferments Mannitol

    • Streptococcus Ferments Mannitol

    • None of the others

    Correct Answer
    A. None of the others
    Explanation
    P 180
    No Growth means the Organism is NOT Staphylococcus

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  • 33. 

    Media: MSA Plate Results: Growth Which of the following can you interpreate from the results about the organism?

    • Staphylococcus

    • Streptococcus

    • Staphylococcus Ferments Mannitol

    • Streptococcus Ferments Mannitol

    • None of the others

    Correct Answer
    A. Staphylococcus
    Explanation
    P 180

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  • 34. 

    Media: MSA Plate Results: Growth with Yellowing of the Medium Which of the following can you interpreate from the results about the organism?

    • Staphylococcus

    • Streptococcus

    • Staphylococcus Ferments Mannitol

    • Streptococcus Ferments Mannitol

    • None of the others

    Correct Answer
    A. Staphylococcus Ferments Mannitol
    Explanation
    P 180

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  • 35. 

    MSA is 

    • Selective Media

    • Differential Media

    • Selective and Differential Media

    • None of the others

    Correct Answer
    A. Selective and Differential Media
    Explanation
    Selective and differential media (SD media) is a type of culture medium that is designed to selectively promote the growth of certain microorganisms while inhibiting the growth of others. It contains specific nutrients or chemicals that favor the growth of particular organisms, allowing them to grow while suppressing the growth of other organisms. Additionally, SD media also contains indicators that can differentiate between different types of microorganisms based on their metabolic activities or biochemical characteristics. Therefore, MSA (Mannitol Salt Agar) can be classified as both selective and differential media as it selects for the growth of salt-tolerant bacteria and differentiates between bacteria based on their ability to ferment mannitol.

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  • 36. 

    MSA stands for

    • Mannitol Salt Algar

    • Media Sulfur Acid

    • My Sweet Alex

    • MultiSellular Actins

    Correct Answer
    A. Mannitol Salt Algar
  • 37. 

    MSA identifies mmembers of

    • Streptococcus

    • Staphylococcus

    • Bacillus

    • Gram Positive

    Correct Answer
    A. Staphylococcus
    Explanation
    MSA (Mannitol Salt Agar) is a selective and differential medium used to identify and isolate Staphylococcus species. It contains high salt concentration, which inhibits the growth of most bacteria except for Staphylococcus, which can tolerate high salt levels. The medium also contains mannitol sugar, which is fermented by certain Staphylococcus species, causing a change in the pH of the agar and resulting in a yellow color change. Therefore, the presence of yellow colonies on MSA indicates the presence of Staphylococcus.

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  • 38. 

    What does Coagulase do?

    • Breaks down Enzyme Coage

    • Clots Plasma

    • Detects Gram Positive Organisms

    • Detects Gram Negative Organisms

    Correct Answer
    A. Clots Plasma
    Explanation
    Coagulase is an enzyme produced by certain bacteria that promotes blood clotting. When present, coagulase causes the conversion of fibrinogen to fibrin, resulting in the formation of a clot. This ability is important for the bacteria's survival as it helps them to evade the host immune response by forming a protective barrier around themselves. Therefore, the correct answer is "Clots Plasma".

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  • 39. 

    Staphylococcus aureus is the only Staph that has

    • Teticohalogen

    • Coagulase

    • Leukocidin

    • Staphylokinase

    Correct Answer
    A. Coagulase
    Explanation
    Coagulase is an enzyme produced by Staphylococcus aureus that is responsible for the formation of fibrin clots. This ability is unique to Staphylococcus aureus and is not found in other staphylococcal species. Coagulase plays a crucial role in the pathogenesis of Staphylococcus aureus infections by helping the bacteria evade the host immune response and form protective barriers.

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  • 40. 

    How Does MSA Work? What makes it Selective?

    • 7.5% NaCl

    • Ph balance of 6.8

    • PH indicator Phenol Red

    • Mannitol

    Correct Answer
    A. 7.5% NaCl
    Explanation
    The correct answer is 7.5% NaCl. This solution is selective because it contains a high concentration of salt (NaCl). This high salt concentration creates a hypertonic environment, which inhibits the growth of most bacteria. Only halophilic (salt-loving) bacteria are able to survive and grow in this selective medium.

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  • 41. 

    How Does MSA Work? What makes it Differential?

    • 7.5% NaCl

    • Ph balance of 6.8

    • PH indicator Phenol Red

    • Mannitol

    Correct Answer
    A. Mannitol
    Explanation
    Mannitol is the component that makes MSA (Mannitol Salt Agar) differential. MSA is a selective and differential medium used for the isolation and differentiation of Staphylococcus aureus from other staphylococci. The high concentration of salt (7.5% NaCl) in MSA inhibits the growth of most bacteria except for staphylococci. Staphylococcus aureus, which can ferment mannitol, produces acid that lowers the pH of the medium, causing a color change in the pH indicator phenol red from red to yellow. Other staphylococci that cannot ferment mannitol do not produce acid and the medium remains red. Therefore, the ability to ferment mannitol differentiates S. aureus from other staphylococci.

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  • 42. 

    MSA has a pH indicator, if the agar turns yellow, the results should be interpreted as

    • Positive for Mannitol

    • Negative for Mannitol

    • Positive for NaCl

    • Negative for NaCl

    Correct Answer
    A. Positive for Mannitol
    Explanation
    If the MSA agar turns yellow, it indicates a low pH level. This change in color suggests that the bacteria present on the agar have fermented the mannitol sugar, producing acid as a byproduct. Therefore, the result is interpreted as positive for mannitol fermentation.

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  • 43. 

    What enzyme degrades hydrogen peroxide to water and oxygen gas?

    Correct Answer
    Catalase
    catalase
    catlase
    Catlase
    Catalace
    Catalace
    Explanation
    Catalase is the correct answer for this question. Catalase is an enzyme that is found in cells and helps to break down hydrogen peroxide into water and oxygen gas. It plays a crucial role in protecting cells from the harmful effects of hydrogen peroxide, which is a byproduct of various metabolic processes. By catalyzing the decomposition of hydrogen peroxide, catalase ensures that it is converted into harmless substances, preventing any damage to the cell.

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  • 44. 

    Catalase Test causes what reaction in Staphs?

    • Bubbling

    • Smoking

    • Stringing

    • Color Change

    • None of the others

    Correct Answer
    A. Bubbling
    Explanation
    The catalase test causes bubbling in Staphs. This reaction occurs because catalase is an enzyme that breaks down hydrogen peroxide into water and oxygen. When hydrogen peroxide is added to a sample containing Staphylococci, the catalase enzyme present in the bacteria catalyzes the breakdown of hydrogen peroxide, resulting in the release of oxygen gas. This release of oxygen gas causes bubbling to occur, indicating a positive catalase test.

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  • 45. 

    Catalase Test causes what reaction in Streps?

    • Bubbling

    • Smoking

    • Stringing

    • Color Change

    • None of the others

    Correct Answer
    A. None of the others
    Explanation
    Catalase has No reaction for Streps

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  • 46. 

    Catalase is a fast test to determine whether an organism is a Staph or Strep

    • True

    • False

    Correct Answer
    A. True
    Explanation
    Catalase is indeed a fast test that can be used to differentiate between Staphylococcus and Streptococcus organisms. This test involves adding hydrogen peroxide to a bacterial culture, and if the bacteria produce the enzyme catalase, they will break down the hydrogen peroxide into water and oxygen, resulting in the formation of bubbles. Staphylococcus species produce catalase, while Streptococcus species do not. Therefore, the statement that catalase is a fast test to determine whether an organism is a Staph or Strep is true.

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  • 47. 

    Staphlycoccus are Gram Positive Cocci 

    • True

    • False

    Correct Answer
    A. False
    Explanation
    p 189

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  • 48. 

    Streptococcus are Gram Positive Cocci 

    • True

    • False

    Correct Answer
    A. True
    Explanation
    p 189

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  • 49. 

    Which is Halophilic

    • Streptococcus

    • Staphylococcus

    Correct Answer
    A. Staphylococcus
    Explanation
    Staphylococcus is halophilic because it is able to tolerate high salt concentrations. This bacterium can survive and thrive in environments with high salt content, such as salty foods or the human skin. Streptococcus, on the other hand, is not halophilic and does not have the ability to tolerate high salt concentrations.

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  • Current Version
  • Dec 14, 2023
    Quiz Edited by
    ProProfs Editorial Team
  • Nov 09, 2011
    Quiz Created by
    Cellascarpi

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