HLAB 2341 Registry Review - Fixation
This is a quiz based on the information in the Fixation powerpoint from my class.
- 1.
In order to obtain adequate test results, the most important decision in the histopathology lab is...
- A.
The steps in processing
- B.
The choice of dye
- C.
The choice of fixative
- D.
The order of staining reagents
Correct Answer
C. The choice of fixativeExplanation
The choice of fixative is the most important decision in the histopathology lab because it determines the preservation and stability of tissue samples. Fixatives are used to prevent tissue degradation, maintain cellular structure, and prevent the growth of microorganisms. The choice of fixative can impact the quality of the samples, the ability to perform accurate diagnoses, and the overall success of the histopathology testing process.Rate this question:
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- 2.
What are the purposes of fixation? (hint: you must choose ALL correct answers to get the question right)
- A.
Minimize processing distortions
- B.
Prevent putrefication, or the destruction of cellular proteins by bacteria.
- C.
Prevent autolysis, or the enzymatic destruction of cells.
- D.
Stabilize proteins.
- E.
Enhances staining when using a mordant like Bouin or Zenker.
Correct Answer(s)
A. Minimize processing distortions
B. Prevent putrefication, or the destruction of cellular proteins by bacteria.
C. Prevent autolysis, or the enzymatic destruction of cells.
D. Stabilize proteins.
E. Enhances staining when using a mordant like Bouin or Zenker.Explanation
Fixation serves several purposes in laboratory techniques such as histology. It helps minimize processing distortions, preventing any changes or artifacts that may occur during the subsequent steps. It also prevents putrefaction, which is the decay or destruction of cellular proteins by bacteria. Additionally, fixation prevents autolysis, the enzymatic destruction of cells that can occur after death. Another purpose is to stabilize proteins, preserving their structure and preventing degradation. Lastly, fixation enhances staining when using a mordant like Bouin or Zenker, improving the visibility and contrast of the tissue samples.Rate this question:
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- 3.
True or False? The main cause for autolysis is delayed fixation.
- A.
True
- B.
False
Correct Answer
A. TrueExplanation
Autolysis refers to the self-digestion of cells or tissues by their own enzymes. Delayed fixation, which refers to a delay in the process of preserving tissues after death, can lead to autolysis. This is because without proper fixation, the enzymes responsible for autolysis are not inactivated, allowing them to break down the cellular components. Therefore, the statement that the main cause for autolysis is delayed fixation is true.Rate this question:
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- 4.
Which of the following is not an additive, or a fixative that binds to tissue to stabilize or change the charge?
- A.
Formaldehyde
- B.
Glutaraldehyde
- C.
Methanol
- D.
Picric acid
- E.
Zinc Salts
Correct Answer
C. MethanolExplanation
Methanol is an alcohol and all alcohols are non-additive fixatives. Non-additive fixatives pull out water, or dehydrates the tissue, but does not bind to the tissue, which then fixes the tissue by making it more stiff.Rate this question:
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- 5.
Which of the following lists includes a non-coagulant fixative, or a fixative which forms a solid gel that may need a vacuum in order for liquid reagents to penetrate during tissue processing?
- A.
Ethanol, Glutaraldehyde, Formaldehyde
- B.
Glutaraldehyde, Mercuric Chloride, Zinc Salts
- C.
Picric Acid, Acetone, Ethanol
- D.
Methanol, Ethanol, Acetone
Correct Answer
A. Ethanol, Glutaraldehyde, FormaldehydeExplanation
Ethanol, Glutaraldehyde, Formaldehyde are all fixatives commonly used in tissue processing. These fixatives do not form a solid gel and do not require a vacuum for liquid reagents to penetrate the tissue.Rate this question:
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- 6.
ALL of the non-additive fixatives will be coagulants; and mercury, zinc, and picric acid are NOT coagulants. True or False?
- A.
True
- B.
False
Correct Answer
B. FalseExplanation
ALL non additives will be coagulants as welll. Mercury, zinc, and picric acid are additive fixative, but will be coagulants as well.Rate this question:
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Mar 21, 2023Quiz Edited by
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Jan 21, 2010Quiz Created by
Shushan09
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