HLAB 2341 Registry Review - Fixation

6 Questions | Total Attempts: 174

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HLAB Quizzes & Trivia

This is a quiz based on the information in the Fixation powerpoint from my class.


Questions and Answers
  • 1. 
    In order to obtain adequate test results, the most important decision in the histopathology lab is...
    • A. 

      The steps in processing

    • B. 

      The choice of dye

    • C. 

      The choice of fixative

    • D. 

      The order of staining reagents

  • 2. 
    What are the purposes of fixation?  (hint: you must choose ALL correct answers to get the question right)
    • A. 

      Minimize processing distortions

    • B. 

      Prevent putrefication, or the destruction of cellular proteins by bacteria.

    • C. 

      Prevent autolysis, or the enzymatic destruction of cells.

    • D. 

      Stabilize proteins.

    • E. 

      Enhances staining when using a mordant like Bouin or Zenker.

  • 3. 
    True or False? The main cause for autolysis is delayed fixation.
    • A. 

      True

    • B. 

      False

  • 4. 
    Which of the following is not an additive, or a fixative that binds to tissue to stabilize or change the charge?
    • A. 

      Formaldehyde

    • B. 

      Glutaraldehyde

    • C. 

      Methanol

    • D. 

      Picric acid

    • E. 

      Zinc Salts

  • 5. 
    Which of the following lists includes a non-coagulant fixative, or a fixative which forms a solid gel that may need a vacuum in order for liquid reagents to penetrate during tissue processing?
    • A. 

      Ethanol, Glutaraldehyde, Formaldehyde

    • B. 

      Glutaraldehyde, Mercuric Chloride, Zinc Salts

    • C. 

      Picric Acid, Acetone, Ethanol

    • D. 

      Methanol, Ethanol, Acetone

  • 6. 
    ALL of the non-additive fixatives will be coagulants; and mercury, zinc, and picric acid are NOT coagulants.  True or False?
    • A. 

      True

    • B. 

      False