HLAB 2341 Registry Review - Fixation

6 Questions | By Shushan09 | Last updated: Jan 9, 2013 | Total Attempts: 179

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This is a quiz based on the information in the Fixation powerpoint from my class.

Questions and Answers

1.

In order to obtain adequate test results, the most important decision in the histopathology lab is...
- A.
  
  The steps in processing
- B.
  
  The choice of dye
- C.
  
  The choice of fixative
- D.
  
  The order of staining reagents
2.

What are the purposes of fixation? (hint: you must choose ALL correct answers to get the question right)
- A.
  
  Minimize processing distortions
- B.
  
  Prevent putrefication, or the destruction of cellular proteins by bacteria.
- C.
  
  Prevent autolysis, or the enzymatic destruction of cells.
- D.
  
  Stabilize proteins.
- E.
  
  Enhances staining when using a mordant like Bouin or Zenker.
3.

True or False? The main cause for autolysis is delayed fixation.
- A.
  
  True
- B.
  
  False
4.

Which of the following is not an additive, or a fixative that binds to tissue to stabilize or change the charge?
- A.
  
  Formaldehyde
- B.
  
  Glutaraldehyde
- C.
  
  Methanol
- D.
  
  Picric acid
- E.
  
  Zinc Salts
5.

Which of the following lists includes a non-coagulant fixative, or a fixative which forms a solid gel that may need a vacuum in order for liquid reagents to penetrate during tissue processing?
- A.
  
  Ethanol, Glutaraldehyde, Formaldehyde
- B.
  
  Glutaraldehyde, Mercuric Chloride, Zinc Salts
- C.
  
  Picric Acid, Acetone, Ethanol
- D.
  
  Methanol, Ethanol, Acetone
6.

ALL of the non-additive fixatives will be coagulants; and mercury, zinc, and picric acid are NOT coagulants. True or False?
- A.
  
  True
- B.
  
  False

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