HLAB 2341 Registry Review - Fixation
6 Questions
| Attempts: 203
This is a quiz based on the information in the Fixation powerpoint from my class.
Questions and Answers
- 1.In order to obtain adequate test results, the most important decision in the histopathology lab is...
- A.
The steps in processing
- B.
The choice of dye
- C.
The choice of fixative
- D.
The order of staining reagents
- 2.What are the purposes of fixation? (hint: you must choose ALL correct answers to get the question right)
- A.
Minimize processing distortions
- B.
Prevent putrefication, or the destruction of cellular proteins by bacteria.
- C.
Prevent autolysis, or the enzymatic destruction of cells.
- D.
Stabilize proteins.
- E.
Enhances staining when using a mordant like Bouin or Zenker.
- 3.True or False? The main cause for autolysis is delayed fixation.
- A.
True
- B.
False
- 4.Which of the following is not an additive, or a fixative that binds to tissue to stabilize or change the charge?
- A.
Formaldehyde
- B.
Glutaraldehyde
- C.
Methanol
- D.
Picric acid
- E.
Zinc Salts
- 5.Which of the following lists includes a non-coagulant fixative, or a fixative which forms a solid gel that may need a vacuum in order for liquid reagents to penetrate during tissue processing?
- A.
Ethanol, Glutaraldehyde, Formaldehyde
- B.
Glutaraldehyde, Mercuric Chloride, Zinc Salts
- C.
Picric Acid, Acetone, Ethanol
- D.
Methanol, Ethanol, Acetone
- 6.ALL of the non-additive fixatives will be coagulants; and mercury, zinc, and picric acid are NOT coagulants. True or False?
- A.
True
- B.
False
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