HLAB 2341 Registry Review - Fixation

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| By Shushan09
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Shushan09
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Quizzes Created: 4 | Total Attempts: 794
Questions: 6 | Attempts: 206

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HLAB Quizzes & Trivia

This is a quiz based on the information in the Fixation powerpoint from my class.


Questions and Answers
  • 1. 

    In order to obtain adequate test results, the most important decision in the histopathology lab is...

    • A.

      The steps in processing

    • B.

      The choice of dye

    • C.

      The choice of fixative

    • D.

      The order of staining reagents

    Correct Answer
    C. The choice of fixative
    Explanation
    The choice of fixative is the most important decision in the histopathology lab because it determines the preservation and stability of tissue samples. Fixatives are used to prevent tissue degradation, maintain cellular structure, and prevent the growth of microorganisms. The choice of fixative can impact the quality of the samples, the ability to perform accurate diagnoses, and the overall success of the histopathology testing process.

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  • 2. 

    What are the purposes of fixation?  (hint: you must choose ALL correct answers to get the question right)

    • A.

      Minimize processing distortions

    • B.

      Prevent putrefication, or the destruction of cellular proteins by bacteria.

    • C.

      Prevent autolysis, or the enzymatic destruction of cells.

    • D.

      Stabilize proteins.

    • E.

      Enhances staining when using a mordant like Bouin or Zenker.

    Correct Answer(s)
    A. Minimize processing distortions
    B. Prevent putrefication, or the destruction of cellular proteins by bacteria.
    C. Prevent autolysis, or the enzymatic destruction of cells.
    D. Stabilize proteins.
    E. Enhances staining when using a mordant like Bouin or Zenker.
    Explanation
    Fixation serves several purposes in laboratory techniques such as histology. It helps minimize processing distortions, preventing any changes or artifacts that may occur during the subsequent steps. It also prevents putrefaction, which is the decay or destruction of cellular proteins by bacteria. Additionally, fixation prevents autolysis, the enzymatic destruction of cells that can occur after death. Another purpose is to stabilize proteins, preserving their structure and preventing degradation. Lastly, fixation enhances staining when using a mordant like Bouin or Zenker, improving the visibility and contrast of the tissue samples.

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  • 3. 

    True or False? The main cause for autolysis is delayed fixation.

    • A.

      True

    • B.

      False

    Correct Answer
    A. True
    Explanation
    Autolysis refers to the self-digestion of cells or tissues by their own enzymes. Delayed fixation, which refers to a delay in the process of preserving tissues after death, can lead to autolysis. This is because without proper fixation, the enzymes responsible for autolysis are not inactivated, allowing them to break down the cellular components. Therefore, the statement that the main cause for autolysis is delayed fixation is true.

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  • 4. 

    Which of the following is not an additive, or a fixative that binds to tissue to stabilize or change the charge?

    • A.

      Formaldehyde

    • B.

      Glutaraldehyde

    • C.

      Methanol

    • D.

      Picric acid

    • E.

      Zinc Salts

    Correct Answer
    C. Methanol
    Explanation
    Methanol is an alcohol and all alcohols are non-additive fixatives. Non-additive fixatives pull out water, or dehydrates the tissue, but does not bind to the tissue, which then fixes the tissue by making it more stiff.

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  • 5. 

    Which of the following lists includes a non-coagulant fixative, or a fixative which forms a solid gel that may need a vacuum in order for liquid reagents to penetrate during tissue processing?

    • A.

      Ethanol, Glutaraldehyde, Formaldehyde

    • B.

      Glutaraldehyde, Mercuric Chloride, Zinc Salts

    • C.

      Picric Acid, Acetone, Ethanol

    • D.

      Methanol, Ethanol, Acetone

    Correct Answer
    A. Ethanol, Glutaraldehyde, Formaldehyde
    Explanation
    Ethanol, Glutaraldehyde, Formaldehyde are all fixatives commonly used in tissue processing. These fixatives do not form a solid gel and do not require a vacuum for liquid reagents to penetrate the tissue.

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  • 6. 

    ALL of the non-additive fixatives will be coagulants; and mercury, zinc, and picric acid are NOT coagulants.  True or False?

    • A.

      True

    • B.

      False

    Correct Answer
    B. False
    Explanation
    ALL non additives will be coagulants as welll. Mercury, zinc, and picric acid are additive fixative, but will be coagulants as well.

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  • Current Version
  • Mar 21, 2023
    Quiz Edited by
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  • Jan 21, 2010
    Quiz Created by
    Shushan09
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