Electrostatic
Covalent
Absorption
Hydrogen
Electrostatic
Absorptive
Covalent
Amphoteric
Anionic
Acidophilic
Basophilic
Polychromatic
Basophilic
Cationic
Acidophilic
Metachromatic
5
7
6
8
Oxidize tissue components
Enhance tissue/dye attraction
Change the refractive index of tissues
Help differentiate stains
Anionic dye
Nuclear stain
Cationic dye
Mordant dye
Auxochrome
Autochrome
Chromophore
Chromeophore
Chromophore
Chromeophore
Autochrome
Auxochrome
-NH
-SO4
-COOH
-OH
Dichromatic
Monochromatic stain
Romanowsky stain
Trichromatic
Azure A and B
Azure 1 and 2
Azure 3 and 4
Azure B and D
Negatively charged
Positively charged
Amphoteric
Metachromatic
The staining intensity is increased as the tissue becomes more negatively charged
No affect at all, eosin is stable across all pH ranges
The staining intensity decreases as the tissue becomes more negatively charged
The staining intensity is decreased due to excess hematoxylin uptake
Acidophilic
Basophilic
Amphoteric
Autofluorescent
Bouins
20% formalin
Glutaraldehyde
Glyoxal
Ripen the hematoxylin
For a dye lake
Make nuclear staining more specific
Blue the hematoxylin
Progressive
Regressive
Indirect 2-tier
Autofluorescence
Weak acid alcohol
Moderate strength acetic acid
Excess mordant, ferric iodine
Excess mordant, ferric chloride
Logwood trees
Lichens
Beetles
Redwood trees
Aluminum-hematoxylin lake
Aluminum-hematein lake
Aluminum-hematin lake
Sodium iodate
Oxidation
Reduction
Hydrolysis
Differentiation
Ammonium aluminum sulfate
Sodium iodate
Ferric chloride
Citric acid
Delafield
Harris
Weigert
Mayer's
Restain the slide with Weigert iron hematoxylin
Nothing can help in this situation
Reprocess the tissue and then rrstain
Restain the slide with Mayer's hematoxylin
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