Exam 1 Nuclear And Cytoplasmic Staining

36 Questions | By Haileyhillman | Updated: Mar 22, 2022 | Attempts: 348

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Exam 1 Nuclear And Cytoplasmic Staining - Quiz

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1.

The most common method of biochemical bonding between tissue proteins and dyes is:
- A.
  
  Electrostatic
- B.
  
  Covalent
- C.
  
  Absorption
- D.
  
  Hydrogen
2.

Most protein molecules are considered _____________ in nature because they are composed of both + and - charged free side chains which are available for biochemical bonding.
- A.
  
  Electrostatic
- B.
  
  Absorptive
- C.
  
  Covalent
- D.
  
  Amphoteric
3.

Most cytoplasmic proteins are
- A.
  
  Anionic
- B.
  
  Acidophilic
- C.
  
  Basophilic
- D.
  
  Polychromatic
4.

Nucleic acids, RNA and DNA and ribosomal RNA are
- A.
  
  Basophilic
- B.
  
  Cationic
- C.
  
  Acidophilic
- D.
  
  Metachromatic
5.

The IEP of proteins in tissue is pH
- A.
  
  5
- B.
  
  7
- C.
  
  6
- D.
  
  8
6.

Mordants are used to
- A.
  
  Oxidize tissue components
- B.
  
  Enhance tissue/dye attraction
- C.
  
  Change the refractive index of tissues
- D.
  
  Help differentiate stains
7.

Eosin is an example of a ___________
- A.
  
  Anionic dye
- B.
  
  Nuclear stain
- C.
  
  Cationic dye
- D.
  
  Mordant dye
8.

Powdered dyes have 2 main molecular components. The component that confers color is called the
- A.
  
  Auxochrome
- B.
  
  Autochrome
- C.
  
  Chromophore
- D.
  
  Chromeophore
9.

Dyes require an ionized group to bind to tissue. This group is called the
- A.
  
  Chromophore
- B.
  
  Chromeophore
- C.
  
  Autochrome
- D.
  
  Auxochrome
10.

Which of the following is NOT an example of an Acid Dye ionic group?
- A.
  
  -NH
- B.
  
  -SO4
- C.
  
  -COOH
- D.
  
  -OH
11.

A stain which has methylene blue and eosin is known as a
- A.
  
  Dichromatic
- B.
  
  Monochromatic stain
- C.
  
  Romanowsky stain
- D.
  
  Trichromatic
12.

Under alkaline conditions, methylene blue breaks down and forms two other distinct dyes. These dyes are called
- A.
  
  Azure A and B
- B.
  
  Azure 1 and 2
- C.
  
  Azure 3 and 4
- D.
  
  Azure B and D
13.

Tissues exposed to acid solutions will become more _______
- A.
  
  Negatively charged
- B.
  
  Positively charged
- C.
  
  Amphoteric
- D.
  
  Metachromatic
14.

What is the affect of the staining quality of eosin if the dye's pH becomes too alkaline?
- A.
  
  The staining intensity is increased as the tissue becomes more negatively charged
- B.
  
  No affect at all, eosin is stable across all pH ranges
- C.
  
  The staining intensity decreases as the tissue becomes more negatively charged
- D.
  
  The staining intensity is decreased due to excess hematoxylin uptake
15.

In comparison to other fixatives, formalin tends to make tissues more _____ by cross-linking to amino groups, thus, lessening the intensity of eosin staining
- A.
  
  Acidophilic
- B.
  
  Basophilic
- C.
  
  Amphoteric
- D.
  
  Autofluorescent
16.

Over fixation in which of the following fixatives will most likely impair nuclear detail?
- A.
  
  Bouins
- B.
  
  20% formalin
- C.
  
  Glutaraldehyde
- D.
  
  Glyoxal
17.

Acetic acid can be added to Harris hematoxylin to
- A.
  
  Ripen the hematoxylin
- B.
  
  For a dye lake
- C.
  
  Make nuclear staining more specific
- D.
  
  Blue the hematoxylin
18.

If the Harris hematoxylin is full strength, without acetic acid added, what is the intended mode of staining?
- A.
  
  Progressive
- B.
  
  Regressive
- C.
  
  Indirect 2-tier
- D.
  
  Autofluorescence
19.

In the VVG elastic tissue stain, Weigert hematoxylin is combined with iodine to stain elastic fibers. What is used to differentiate the tissue to clearly demonstrate the elastic fibers?
- A.
  
  Weak acid alcohol
- B.
  
  Moderate strength acetic acid
- C.
  
  Excess mordant, ferric iodine
- D.
  
  Excess mordant, ferric chloride
20.

Hematoxylin is extracted from
- A.
  
  Logwood trees
- B.
  
  Lichens
- C.
  
  Beetles
- D.
  
  Redwood trees
21.

The active staining ingredient in ripened hematoxylin solutions is
- A.
  
  Aluminum-hematoxylin lake
- B.
  
  Aluminum-hematein lake
- C.
  
  Aluminum-hematin lake
- D.
  
  Sodium iodate
22.

Ripening of hematoxylin is a process of
- A.
  
  Oxidation
- B.
  
  Reduction
- C.
  
  Hydrolysis
- D.
  
  Differentiation
23.

Harris hematoxylin is converted into a dye lake by adding
- A.
  
  Ammonium aluminum sulfate
- B.
  
  Sodium iodate
- C.
  
  Ferric chloride
- D.
  
  Citric acid
24.

You have chosen to perform a special stain procedure that uses several acid reagents. Of the choices below, which hematoxylin stain will best resist decolorization?
- A.
  
  Delafield
- B.
  
  Harris
- C.
  
  Weigert
- D.
  
  Mayer's
25.

What method can enhance pale nuclear staining of over decalcified bone?
- A.
  
  Restain the slide with Weigert iron hematoxylin
- B.
  
  Nothing can help in this situation
- C.
  
  Reprocess the tissue and then rrstain
- D.
  
  Restain the slide with Mayer's hematoxylin

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