Histopathology Quiz Questions And Answers

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Histopathology Quiz Questions And Answers - Quiz

Test your knowledge of histopathology with this quiz that includes informative questions and answers today! Do you feel that you can pass this quiz? Histopathology is the microscopic examination of tissue to study all of the elements of the disease. Specifically, in clinical medicine, histopathology refers to the study of a biopsy or surgical specimen by a pathologist after the specimen. Try this quiz and see how much you actually know about this subject. All the best!


Questions and Answers
  • 1. 

    Which is not the aim of fixation?

    • A.

      To prevent putrefaction

    • B.

      Remove excess water from the tissue

    • C.

      Prevent osmotic swelling

    • D.

      Render the tissue suitable for subsequent staining

    • E.

      To prevent lysosomal activity

    Correct Answer
    D. Render the tissue suitable for subsequent staining
    Explanation
    Fixation is a process used in histology to preserve tissue samples for further analysis. The aim of fixation is to prevent putrefaction, remove excess water from the tissue, prevent osmotic swelling, and prevent lysosomal activity. Rendering the tissue suitable for subsequent staining is not the aim of fixation. Fixation helps to preserve the structure and cellular components of the tissue, making it easier to analyze and study under a microscope. Staining is a separate step in the histology process that involves adding dyes to highlight specific structures or cells in the tissue sample.

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  • 2. 

    Which statement about Formaldehyde is false?

    • A.

      Most commonly used Aldehyde fixative

    • B.

      It is a water soluble gas (sat. approx. 40%)

    • C.

      Concentrated formaldehyde is 40%

    • D.

      Penetrates tissue rapidly

    • E.

      Fixes tissue rapidly

    • F.

      The best fixative for nucleic acids

    Correct Answer
    F. The best fixative for nucleic acids
    Explanation
    Formaldehyde is a commonly used aldehyde fixative, but it is not the best fixative for nucleic acids. Other fixatives, such as ethanol or methanol, are more effective in preserving nucleic acids.

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  • 3. 

    Which statement about Glutaraldehyde is false?

    • A.

      Widely used in EM

    • B.

      Gives a better morphological picture at the EM level

    • C.

      Cross-links less extensively than formaldehyde

    • D.

      Diffuses into tissue very slowly

    • E.

      Fixes tissue components rapidly

    • F.

      Renders tissue too hard for paraffin sectioning

    Correct Answer
    C. Cross-links less extensively than formaldehyde
    Explanation
    Glutaraldehyde cross-links tissue components extensively, making it a better fixative for preserving the morphology of tissues at the electron microscopy (EM) level compared to formaldehyde. This statement implies that glutaraldehyde does not cross-link as extensively as formaldehyde, which is false. Glutaraldehyde actually cross-links more extensively than formaldehyde, resulting in better preservation of tissue structure at the EM level.

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  • 4. 

    Mercuric Chloride is restricted in use because:

    • A.

      It's toxic

    • B.

      Its corrosive

    • C.

      Its a pollutant

    • D.

      All of the above

    Correct Answer
    D. All of the above
    Explanation
    Mercuric chloride is restricted in use because it is toxic, corrosive, and a pollutant. Being toxic means that it can cause harm or even be fatal if ingested, inhaled, or absorbed through the skin. Its corrosive nature means that it can cause severe damage to tissues and materials it comes into contact with. Additionally, it is considered a pollutant because it can contaminate the environment and have harmful effects on ecosystems and human health. Therefore, due to its toxic, corrosive, and polluting properties, mercuric chloride is restricted in use.

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  • 5. 

    Formalin Solution (10%, unbuffered) is composed of:

    • A.

      10ml Formaldehyde (40%) and 90 ml distilled water

    • B.

      50ml Formaldehyde (40%) and 50 ml distilled water

    • C.

      90ml Formaldehyde (10%) and 10 ml distilled water

    • D.

      90ml Formaldehyde (10%) and 10 ml distilled water

    Correct Answer
    A. 10ml Formaldehyde (40%) and 90 ml distilled water
    Explanation
    Formalin Solution (10%, unbuffered) is composed of 10 ml Formaldehyde (40%) and 90 ml distilled water. This means that the solution contains 10% formaldehyde, which is achieved by diluting 10 ml of a 40% formaldehyde solution with 90 ml of distilled water. The other options either have different concentrations of formaldehyde or incorrect proportions of formaldehyde and water.

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  • 6. 

    Which is not an Aldehyde fixative?

    • A.

      Picric Acid

    • B.

      Potassium Dichromate

    • C.

      Glutaldehyde

    • D.

      Mercuric chloride

    Correct Answer
    B. Potassium Dichromate
    Explanation
    Potassium Dichromate is not an Aldehyde fixative because it is a strong oxidizing agent that reacts with aldehydes, converting them into carboxylic acids. Aldehyde fixatives are used to preserve and stabilize tissue samples by cross-linking proteins, and they typically contain aldehydes such as formaldehyde or glutaraldehyde. Picric Acid, Glutaldehyde, and Mercuric chloride are all examples of aldehyde fixatives commonly used in histology and pathology laboratories.

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  • 7. 

    Why isn't microwave fixation suitable for large specimens (of tissues/organs)?

    • A.

      Microwave fixation is best suited on tissue blocks 10-12mm thick

    • B.

      The microwave radiation can damage fine tissue structures

    • C.

      It is unable to heat the center of the tissue/organ sufficiently without overheating the outside

    • D.

      Using microwaves accelerates the rate of putrefaction

    Correct Answer
    C. It is unable to heat the center of the tissue/organ sufficiently without overheating the outside
    Explanation
    Microwave fixation is not suitable for large specimens because it is unable to heat the center of the tissue or organ sufficiently without overheating the outside. This can lead to uneven fixation and potential damage to the tissue structure.

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  • 8. 

    Acetic acid...

    • A.

      Is a denaturing agent

    • B.

      Swells collagen

    • C.

      Is a precipitating fixative

    • D.

      Is an alcohol

    • E.

      Is all of the above

    Correct Answer
    E. Is all of the above
    Explanation
    Acetic acid is a versatile compound that can act as a denaturing agent, meaning it can disrupt the structure and function of proteins. It can also swell collagen, which is a fibrous protein found in connective tissues. Additionally, acetic acid can be used as a precipitating fixative, meaning it can cause proteins to precipitate out of solution. Lastly, acetic acid is a type of alcohol. Therefore, the correct answer is that acetic acid is all of the above.

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  • 9. 

    Microwave fixation fixes blocks in normal saline solution at  ___°C for ___ seconds.

    • A.

      74;60

    • B.

      26;20

    • C.

      62;30

    • D.

      50;25

    Correct Answer
    C. 62;30
    Explanation
    Microwave fixation is a method used to fix blocks in a normal saline solution. The correct answer for the temperature and time of fixation is 62°C for 30 seconds. This specific temperature and time combination ensures effective fixation of the blocks, preserving their structure and preventing degradation.

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  • 10. 

    Which is NOT an essential of good fixation?

    • A.

      Fix it ASAP

    • B.

      Refrigerate it if fixation isn't immediately possible

    • C.

      Reagents are carefully made up

    • D.

      Fixative is recycled and reused

    • E.

      A suitable treatment follows fixation

    Correct Answer
    D. Fixative is recycled and reused
    Explanation
    The correct answer is "Fixative is recycled and reused". This is because recycling and reusing fixative can lead to contamination and inconsistent results. Fixative is used to preserve and stabilize biological samples, so it is important to use fresh fixative each time to ensure proper fixation. Recycling and reusing fixative can introduce impurities and compromise the quality of the fixation process.

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Nicolette Natale |BA |
Medical Expert
Nicolette is an accomplished physician, research coordinator, and medical writer, boasting over 6 years of expertise in healthcare, research, psychology, and education. Her qualifications include a D.O. from Nova Southeastern University and B.A. degrees in English Literature and Psychology from the University of Miami. Nicolette is deeply involved in medical research and patient care, demonstrating a commitment to advancing the field of medicine.
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