1.
A segment of image is magnified by the eyepiece(ocular).
What is the best resolving power ?
A. 
B. 
C. 
D. 
2.
How thick are paraffin embedded sections that has been cut ?
A. 
B. 
C. 
D. 
3.
How can you achieve better resolution in microscopy ?
A. 
Using paraffin-embedded sections
B. 
Using thinner resin-embedded sections
C. 
Using formaldehyde-embedded sections
D. 
Using a mixture of paraffin and resin
4.
What are epoxy resin sections stained with after being cut with a microtome ?
A. 
B. 
C. 
D. 
5.
What staining is best for lipid detection ?
A. 
B. 
C. 
Feulgens nuclear reaction
D. 
6.
What method is used for: observation of unstained structures in dark field or using phase contrast (tissue
cultures)
A. 
B. 
C. 
D. 
7.
What is advantages and disadvantages of Frozen sections cut by microtomes ?
A. 
Disadvantage: Shrinkage of tissue
B. 
Disadvantage: it is impossible to prepare think sections
C. 
Advantage: the use of fat solvents can be avoided
D. 
Disadvantage: Inactivation of enzymes
E. 
Sections may be stained with many methods
8.
Paraffin embedded sections(part2): The block of tissue is dehydrated by passing the tissue through a series of graded ethanols up to 100% plus/or acetone prior to its embedding in paraffin.
Then ethanol must be replaced by a non-polar solvent of paraffin such as xylen or benzene
Knowing the facts from (part1) what are the disadvantages of paraffin embedding ?
A. 
It is impossible to prepare thin-sections
B. 
The sections often become cracked
C. 
D. 
The untreated sections cannot be stored permanently
E. 
Lipids cannot be detected
F. 
The enzymes become inactivated
9.
Paraffin-embedded sections(part 3): These solvents also render the tissue translucent(the so called clearing).
The tissue is then placed in melted paraffin(56 c) and in the infiltration chamber, it is embedded in paraffin.
After cooling the paraffin hardens and gives rise to the paraffin block, which is ready for sectioning.
Sections are put on the glass plates, stretched and attached with the gelatine or a mixture of the white egg and glycerol(ratio 1:1)
Which solvents render the tissue translucent ?
A. 
B. 
C. 
D. 
E. 
10.
What is the temperature of the paraffin the tissue is placed in ?
A. 
B. 
C. 
D. 
11.
4) Staining the sections
First sections must be deparaffinized in xylene and rehydrate through a series of decreasing concentrations of ethanol and finally rinsed out in Distilled water.
12.
What color will Haemotoxylins stain the nuclei ?
A. 
B. 
C. 
D. 
13.
II) Plasmatic Aniline dyes (acidic) bind to basic tissue structures(cytoplasm, collagen, mucus, fibrin, elastic fibres, etc) i.e acidophilic structures.
- Eosin: colours structures pinkish; granules of eosinophilic leukocytes, acidophilic cells in the Pituitary gland, erythrocytes etc. are stained very intensively red.
A general tissue staining: Haematoxylin and eosin(HE)
-Picric acid: in van Giesons stain only
14.
When using Nissl staining
Nissl substance in the CNS get the following color:
15.
When using Nissl staining in the CNS nuclei get the following color:
A. 
B. 
C. 
D. 
16.
Whats a general tissue staining ?
A. 
Haematoxylin and eosin(HE)
B. 
C. 
17.
Nuclear red is:
A. 
B. 
C. 
Used to stain reticular fibres
D. 
Used to stain myelin sheats
E. 
Used to stian Haemosiderin
18.
Paraffin embedded sections of tissue are cut by __________.
A. 
B. 
C. 
D. 
19.
Paraffin-embedded sections. Advantage: Relatively thin sections can be prepared from a block of tissue embedded in paraffin.
Sections may be stained with many methods. The microscopic structure of the tissue is well preserved.
Sections and blocks can be activated without any change for decades.
Disadvantage: Shrinkage of the tissue, inactivation of enzymes, lipids cannot be detected
20.
The use of fat solvents can be avoided using the following sectioning:
A) Frozen section
B) Paraffin-embedded section
Write the right one.
21.
1) Nucleic(BASIC) stains
bind to acidic tissue structures(chromatin of the nucleus, ergastoplasm, ground substance of the cartliage) i.e basophilic structures.
Haemotoxylins: several variants exist(after oxidation and addition of various mordrants). Nuclei= dark
Nuclear red: nuclei = red
Basic aniline dyes: e.g cresyl violet, toluidine blue
They stain ergastoplasm in a different shade ( = metachromatically) than nuclei.
Nissl staining( in the nervous system): nuclei = greenish blue, Nissl substance = reddish
22.
In Neurohistology:
Which of the following is used to stain Myelin sheats?
A. 
B. 
C. 
D. 
E. 
F. 
23.
In pigment staining
What is used for staining Haemosiderin?
A. 
B. 
C. 
D. 
E. 
Weigert resorcin-fuchsin technique
24.
Neutral lips are stained with ___________.
A. 
B. 
C. 
D. 
E.