Histopathology Techniques Quiz With Answers

24 Questions | By Ferna | Last updated: Mar 22, 2022 | Total Attempts: 14996

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Histopathology Techniques Quiz With Answers - Quiz

Here is an interesting quiz about ' histopathology techniques' that will test your knowledge about this topic. So, if you think you're really good at this subject, then you must take this quiz. Here, we will ask you a few simple questions and you've to pick the option that you think is correct. We will display the results in the end, and you can check how well you scored. Sounds cool, right? So, let's start this quiz then. Wishing you the best of luck!

Questions and Answers

1.

A segment of image is magnified by the eyepiece(ocular). What is the best resolving power ?
- A.
  
  0.1 nM
- B.
  
  0.4 mm
- C.
  
  0.2 uM
- D.
  
  3.0 uM
2.

How thick are paraffin embedded sections that has been cut ?
- A.
  
  5-8 uM
- B.
  
  5-8 nM
- C.
  
  3-4 mm
- D.
  
  3-4 cm
3.

How can you achieve better resolution in microscopy ?
- A.
  
  Using paraffin-embedded sections
- B.
  
  Using thinner resin-embedded sections
- C.
  
  Using formaldehyde-embedded sections
- D.
  
  Using a mixture of paraffin and resin
4.

What are epoxy resin sections stained with after being cut with a microtome ?
- A.
  
  Blue trichrome
- B.
  
  Hematoxylin and eosin
- C.
  
  Sudan III
- D.
  
  Toluidine blue
5.

What staining is best for lipid detection ?
- A.
  
  Sudan III IV
- B.
  
  Berlin blue
- C.
  
  Feulgens nuclear reaction
- D.
  
  Colloid gold
6.

What method is used for: observation of unstained structures in dark field or using phase contrast (tissue cultures)
- A.
  
  Electron microscopy
- B.
  
  Transmission EM
- C.
  
  Light microscopy
- D.
  
  Autoradiography
7.

What is advantages and disadvantages of Frozen sections cut by microtomes ?
- A.
  
  Disadvantage: Shrinkage of tissue
- B.
  
  Disadvantage: it is impossible to prepare think sections
- C.
  
  Advantage: the use of fat solvents can be avoided
- D.
  
  Disadvantage: Inactivation of enzymes
- E.
  
  Sections may be stained with many methods
8.

Paraffin embedded sections(part2): The block of tissue is dehydrated by passing the tissue through a series of graded ethanols up to 100% plus/or acetone prior to its embedding in paraffin. Then ethanol must be replaced by a non-polar solvent of paraffin such as xylen or benzene Knowing the facts from (part1) what are the disadvantages of paraffin embedding ?
- A.
  
  It is impossible to prepare thin-sections
- B.
  
  The sections often become cracked
- C.
  
  The tissue shrinks
- D.
  
  The untreated sections cannot be stored permanently
- E.
  
  Lipids cannot be detected
- F.
  
  The enzymes become inactivated
9.

Paraffin-embedded sections(part 3): These solvents also render the tissue translucent(the so called clearing). The tissue is then placed in melted paraffin(56 c) and in the infiltration chamber, it is embedded in paraffin. After cooling the paraffin hardens and gives rise to the paraffin block, which is ready for sectioning. Sections are put on the glass plates, stretched and attached with the gelatine or a mixture of the white egg and glycerol(ratio 1:1) Which solvents render the tissue translucent ?
- A.
  
  Ethanol
- B.
  
  Xylen
- C.
  
  Benzene
- D.
  
  Paraffin
- E.
  
  Eosin
10.

What is the temperature of the paraffin the tissue is placed in ?
- A.
  
  76 c
- B.
  
  35 c
- C.
  
  56 c
- D.
  
  70 c
11.

4) Staining the sections First sections must be deparaffinized in xylene and rehydrate through a series of decreasing concentrations of ethanol and finally rinsed out in Distilled water.
- A.
  
  True
- B.
  
  False
12.

What color will Haemotoxylins stain the nuclei ?
- A.
  
  Red
- B.
  
  Dark
- C.
  
  Greenish blue
- D.
  
  Reddish
13.

II) Plasmatic Aniline dyes (acidic) bind to basic tissue structures(cytoplasm, collagen, mucus, fibrin, elastic fibres, etc) i.e acidophilic structures. - Eosin: colours structures pinkish; granules of eosinophilic leukocytes, acidophilic cells in the Pituitary gland, erythrocytes etc. are stained very intensively red. A general tissue staining: Haematoxylin and eosin(HE) -Picric acid: in van Giesons stain only
- A.
  
  True
- B.
  
  False
14.

When using Nissl staining Nissl substance in the CNS get the following color:
- A.
  
  Red
- B.
  
  Blue
- C.
  
  Greenish
15.

When using Nissl staining in the CNS nuclei get the following color:
- A.
  
  Dark
- B.
  
  Reddish
- C.
  
  Greenish blue
- D.
  
  Yellow
16.

Whats a general tissue staining ?
- A.
  
  Haematoxylin and eosin(HE)
- B.
  
  Toluidine blue
- C.
  
  Sudan IV and eosin
17.

Nuclear red is:
- A.
  
  Basic stain
- B.
  
  Acidic stain
- C.
  
  Used to stain reticular fibres
- D.
  
  Used to stain myelin sheats
- E.
  
  Used to stian Haemosiderin
18.

Paraffin embedded sections of tissue are cut by __________.
- A.
  
  Electron beams
- B.
  
  Sharp knife
- C.
  
  Laser
- D.
  
  Microtome
19.

Paraffin-embedded sections. Advantage: Relatively thin sections can be prepared from a block of tissue embedded in paraffin. Sections may be stained with many methods. The microscopic structure of the tissue is well preserved. Sections and blocks can be activated without any change for decades. Disadvantage: Shrinkage of the tissue, inactivation of enzymes, lipids cannot be detected
- A.
  
  True
- B.
  
  False
20.

The use of fat solvents can be avoided using the following sectioning: A) Frozen section B) Paraffin-embedded section Write the right one.
21.

1) Nucleic(BASIC) stains bind to acidic tissue structures(chromatin of the nucleus, ergastoplasm, ground substance of the cartliage) i.e basophilic structures. Haemotoxylins: several variants exist(after oxidation and addition of various mordrants). Nuclei= dark Nuclear red: nuclei = red Basic aniline dyes: e.g cresyl violet, toluidine blue They stain ergastoplasm in a different shade ( = metachromatically) than nuclei. Nissl staining( in the nervous system): nuclei = greenish blue, Nissl substance = reddish
- A.
  
  True
- B.
  
  False
22.

In Neurohistology: Which of the following is used to stain Myelin sheats?
- A.
  
  Cresyl violet
- B.
  
  Best carmien
- C.
  
  Perls reaction
- D.
  
  Sudan III
- E.
  
  Canada balsam
- F.
  
  Luxol blue
23.

In pigment staining What is used for staining Haemosiderin?
- A.
  
  Perl´s reaction
- B.
  
  Nuclear red
- C.
  
  Best carmine
- D.
  
  Green trichrome
- E.
  
  Weigert resorcin-fuchsin technique
24.

Neutral lips are stained with ___________.
- A.
  
  Haematoxylin
- B.
  
  Eosin
- C.
  
  Sudan III
- D.
  
  Sudan IV
- E.
  
  Auric chloride