Histopathology Techniques Quiz Questions And Answers
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Try out this quiz based on histopathology techniques and give correct answers to the questions asked here to evaluate your knowledge regarding histology lab techniques. Histopathology is a branch of pathology that deals with the study of microscopic examination of the diseases of the tissues. The quiz below asks some important questions related to the techniques and equipment used in histopathology. If you want to explore more about these techniques, you can take up this test and learn about them.
- 1.Dyes and Stains are:
- A.
Essentially aromatic benzene ring components
- B.
Possess the properties of color and ability to bind to tissue
- C.
Both
- D.
Neither
- 2.What is the integral component of a dye that is responsible for altering light resonance?
- A.
Chromophore
- B.
Auxochrome
- C.
Both
- D.
Neither
- 3.Cresyl fast violet is an example of
- A.
Quinoid ring
- B.
Quinone Imine Groups
- C.
Both
- D.
Neither
- 4.Hematoxylin:
- A.
Stains the nucleus
- B.
Stains the cytoplasm
- C.
Is negatively charged
- D.
All of the above
- E.
B and C only
- 5.What is the heartwood from which hematoxylin comes from?
- 6.What type of synthetic dye is EOSIN
- A.
Azo dye
- B.
Triphenylmethane
- C.
Thiazine dye
- D.
Xanthene dye
- E.
Oxazine dye
- 7.What type of synthetic dye is crystal violet?
- A.
Azo dye
- B.
Triphenylmethane
- C.
Thiazine dye
- D.
Xanthene dye
- E.
Oxazine dye
- 8.What type of dye is THIONINE?
- A.
Azo dye
- B.
Triphenylmethane
- C.
Thiazine dye
- D.
Xanthene dye
- E.
Oxazine dye
- 9.Janus green is a vitally stains:
- A.
Nucleus
- B.
Cytoplasm
- C.
RES
- D.
Mitochondria
- E.
Chromatin
- 10.Staining with a simple aqueous or alcoholic solution like Methylene Blue is an example of:
- A.
Vital staining
- B.
Routine staining
- C.
Direct staining
- D.
Indirect staining
- E.
Progressive staining
- F.
Regressive staining
- 11.Iron hematoxylin:
- A.
Enhances the combination of the dye with the nucleus
- B.
Colors the nucleus black
- C.
Used for progressive staining
- D.
All of the above
- E.
A and B
- 12.Glacial HAc in hematoxylin is an example of a/an
- A.
Mordant
- B.
Accentuator
- C.
Accelerator
- D.
All of the above
- E.
B and C
- 13.Association: DECOLORIZATION
- A.
Progressive staining
- B.
Regressive staining
- C.
Both
- D.
Neither
- 14.Association: Dye will not be the color seen in the tissue
- A.
Progressive staining
- B.
Regressive staining
- C.
Both
- D.
Neither
- 15.In ripening/oxidation of hematoxylin, an alkaline pH will oxidize it:
- A.
In a few hours
- B.
More slowly
- C.
More rapidly
- D.
None of the above
- 16.Which will color the nuclues BLUE?(multiple answers)
- A.
Harris Hematoxylin
- B.
Wiegart's Hematoxylin
- C.
Ehrlich's Hematoxylin
- D.
Heidenhain's Hematoxylin
- E.
Delafield's Hematoxylin
- F.
Mallory's Hematoxylin
- 17.Which component substance in Harris Hematoxylin is not a mordant but increases staining instensity?
- A.
K alum
- B.
Mercuric oxide
- C.
95% ethanol
- D.
Glacial HAc
- E.
A and B
- 18.Eosin is widely used as an aqueous or alcoholic solution with a concentration of:
- A.
0.1-0.5%
- B.
0.5-1%
- C.
1-2%
- D.
5-10%
- E.
70-90%
- 19.For how long does the acidified counterstain, eosin, give a good result before its pH rises?
- A.
1 day
- B.
5 days
- C.
1 week
- D.
2 weeks
- E.
4 weeks
- 20.The commonly used kind of Eosin is: (multiple answers)
- A.
Eosin Y
- B.
Eosin B
- C.
Bluish
- D.
Yellowish
- 21.The higher the concentration of the stain, the more the ionization
- A.
True
- B.
False
- 22.The optimum pH is the pH at which each molecule has an equal number of (+) and (-) charges
- A.
True
- B.
False
- 23.Formalin fixative increases affinity of chemical compound for:
- A.
Acid stain
- B.
Basic stain
- C.
Both
- D.
Neither
- 24.TRUE for deparaffinization:
- A.
Makes stain permeate tissue section with highly organic solvents
- B.
1-2 changes of alcohol for 1 min
- C.
Uses 95% ehanol
- D.
All of the above
- E.
B and C only
- 25.The most commonly used agent for removal of alcohol, making tissue transparent
- A.
Ethanol
- B.
Paraffin
- C.
Xylene
- D.
Acetone
- E.
H&E
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