Histopathology Techniques Quiz Questions And Answers

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Histopathology Techniques Quiz Questions And Answers - Quiz


Try out this quiz based on histopathology techniques and give correct answers to the questions asked here to evaluate your knowledge regarding histology lab techniques. Histopathology is a branch of pathology that deals with the study of microscopic examination of the diseases of the tissues. The quiz below asks some important questions related to the techniques and equipment used in histopathology. If you want to explore more about these techniques, you can take up this test and learn about them.


Questions and Answers
  • 1. 

    Which of the following are TRUE of staining? (multiple answers)

    • A.

      Shows a hypochromic characteristic for neoplastic and malignant cells.

    • B.

      It is the process that renders the different tissue components more visible through variation in color.

    • C.

      Promotes easier optical dfferentiation.

    • D.

      Sensitivity is the quality of sucessful staining which describes its ability to color one component and leaving others unstained.

    • E.

      Accomplished by the use of dyes and stains.

    Correct Answer(s)
    B. It is the process that renders the different tissue components more visible through variation in color.
    C. Promotes easier optical dfferentiation.
    E. Accomplished by the use of dyes and stains.
    Explanation
    Staining is a process that renders the different tissue components more visible through variation in color. This is achieved by using dyes and stains, which promote easier optical differentiation. Sensitivity is not a characteristic of successful staining, as it refers to the ability to color one component while leaving others unstained. The statement about hypochromic characteristic for neoplastic and malignant cells is not true, as staining does not specifically show this characteristic.

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  • 2. 

    Principles of histological technique for distinction of tissue components are based on ____?

    • A.

      Alteration of contrast

    • B.

      Alteration of color

    • C.

      Both

    • D.

      Neither

    Correct Answer
    C. Both
    Explanation
    The principles of histological technique for distinction of tissue components are based on both alteration of contrast and alteration of color. Alteration of contrast involves enhancing the differences in intensity between different tissue components, making them easier to distinguish. Alteration of color involves using stains or dyes to selectively color different tissue components, highlighting their differences in color. By combining these two principles, histological techniques can effectively differentiate between different tissue components.

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  • 3. 

    Dyes and Stains are:

    • A.

      Essentially aromatic benzene ring components

    • B.

      Possess the properties of color and ability to bind to tissue

    • C.

      Both

    • D.

      Neither

    Correct Answer
    C. Both
    Explanation
    Dyes and stains are both essentially aromatic benzene ring components and possess the properties of color and ability to bind to tissue. This means that they are capable of providing color to substances and have the ability to bind to tissues, making them useful in various applications such as coloring fabrics or staining biological samples for microscopic examination.

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  • 4. 

    What is the integral component of a dye that is responsible for altering light resonance?

    • A.

      Chromophore

    • B.

      Auxochrome

    • C.

      Both

    • D.

      Neither

    Correct Answer
    A. Chromophore
    Explanation
    The integral component of a dye that is responsible for altering light resonance is known as a chromophore. A chromophore is a chemical group or moiety within a molecule that absorbs certain wavelengths of light and gives the dye its characteristic color. It is the presence of the chromophore that allows the dye to interact with light and produce the desired color. An auxochrome, on the other hand, is a functional group that modifies the color of the chromophore but is not essential for altering light resonance. Therefore, the correct answer is Chromophore.

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  • 5. 

    Cresyl fast violet is an example of

    • A.

      Quinoid ring

    • B.

      Quinone Imine Groups

    • C.

      Both

    • D.

      Neither

    Correct Answer
    B. Quinone Imine Groups
    Explanation
    Cresyl fast violet contains quinone imine groups. Quinone imine groups are a type of chemical structure that contain both a quinone and an imine functional group. Therefore, cresyl fast violet can be categorized as an example of quinone imine groups.

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  • 6. 

    Hematoxylin:

    • A.

      Stains the nucleus

    • B.

      Stains the cytoplasm

    • C.

      Is negatively charged

    • D.

      All of the above

    • E.

      B and C only

    Correct Answer
    A. Stains the nucleus
    Explanation
    Hematoxylin is a dye commonly used in histology to stain the nucleus of cells. It has an affinity for DNA and RNA, allowing it to bind and color the nucleus. The other options, staining the cytoplasm and being negatively charged, are incorrect as hematoxylin specifically targets the nucleus. Therefore, the correct answer is that hematoxylin stains the nucleus.

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  • 7. 

    What is the heartwood from which hematoxylin comes from?

    Correct Answer
    Hematoxylon campechianum
    Explanation
    Hematoxylin is derived from the heartwood of the Hematoxylon campechianum tree. The heartwood refers to the inner, darker, and non-living part of the tree trunk, which is typically harder and more durable than the outer sapwood. Hematoxylin is a natural dye extracted from the heartwood of this particular tree species and is commonly used in histology and staining techniques.

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  • 8. 

    What type of synthetic dye is EOSIN

    • A.

      Azo dye

    • B.

      Triphenylmethane

    • C.

      Thiazine dye

    • D.

      Xanthene dye

    • E.

      Oxazine dye

    Correct Answer
    D. Xanthene dye
    Explanation
    EOSIN is classified as a xanthene dye. Xanthene dyes are a type of synthetic dye that are characterized by their xanthene ring structure. They are commonly used as fluorescent dyes in various applications, including microscopy and histology. EOSIN, specifically, is a red dye that is often used as a counterstain in histological staining procedures. It is known for its ability to stain cytoplasm and extracellular connective tissue, making it useful for highlighting specific structures in tissue samples.

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  • 9. 

    What type of synthetic dye is crystal violet?

    • A.

      Azo dye

    • B.

      Triphenylmethane

    • C.

      Thiazine dye

    • D.

      Xanthene dye

    • E.

      Oxazine dye

    Correct Answer
    B. Triphenylmethane
    Explanation
    Crystal violet is classified as a triphenylmethane dye. Triphenylmethane dyes are a class of synthetic dyes that contain a central triphenylmethane structure. Crystal violet is a dark green powder that is commonly used as a biological stain and as a dye in various industries. Its triphenylmethane structure gives it its characteristic color and chemical properties, making it suitable for a range of applications.

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  • 10. 

    What type of dye is THIONINE?

    • A.

      Azo dye

    • B.

      Triphenylmethane

    • C.

      Thiazine dye

    • D.

      Xanthene dye

    • E.

      Oxazine dye

    Correct Answer
    C. Thiazine dye
    Explanation
    Thionine is classified as a thiazine dye because it contains a thiazine ring structure in its chemical composition. Thiazine dyes are a class of synthetic dyes that are commonly used in various applications such as textile dyeing, biological staining, and as sensitizers in photography. Thionine, specifically, is a blue-violet dye that is often used in histology to stain cell nuclei.

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  • 11. 

    Janus green is a vitally stains:

    • A.

      Nucleus

    • B.

      Cytoplasm

    • C.

      RES

    • D.

      Mitochondria

    • E.

      Chromatin

    Correct Answer
    D. Mitochondria
    Explanation
    Janus green is a vital stain that is commonly used to stain mitochondria in cells. Vital stains are dyes that can selectively stain specific structures or organelles within cells. In the case of Janus green, it has an affinity for mitochondria and can specifically stain these organelles. This staining allows researchers to visualize and study the morphology and function of mitochondria within cells. Therefore, the correct answer for this question is mitochondria.

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  • 12. 

    Staining with a simple aqueous or alcoholic solution like Methylene Blue is an example of:

    • A.

      Vital staining

    • B.

      Routine staining

    • C.

      Direct staining

    • D.

      Indirect staining

    • E.

      Progressive staining

    • F.

      Regressive staining

    Correct Answer
    C. Direct staining
    Explanation
    Direct staining refers to a staining technique where the dye is applied directly to the specimen, without any prior treatment or fixation. In this case, staining with a simple aqueous or alcoholic solution like Methylene Blue is an example of direct staining because the dye is directly applied to the specimen without any additional steps. This technique is commonly used in microbiology to visualize microorganisms under a microscope.

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  • 13. 

    Iron hematoxylin:

    • A.

      Enhances the combination of the dye with the nucleus

    • B.

      Colors the nucleus black

    • C.

      Used for progressive staining

    • D.

      All of the above

    Correct Answer
    D. All of the above
    Explanation
    Iron hematoxylin is a staining technique used in histology to visualize structures within cells, particularly the nucleus. It has the following features:
    Enhances the combination of the dye with the nucleus: Iron hematoxylin enhances the binding of the stain to nuclear material, allowing for better visualization of cell nuclei.
    Colors the nucleus black: This staining technique imparts a dark color, typically black, to the nuclei, providing a clear contrast with other cell components.
    Used for progressive staining: Iron hematoxylin can be used for progressive staining, where the staining process is carefully controlled to achieve the desired intensity without overstaining.
    Thus, iron hematoxylin fulfills all these characteristics, making "all of the above" the correct answer. This staining method is useful in histological studies where precise and clear visualization of cellular structures is required.

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  • 14. 

    Glacial HAc in hematoxylin is an example of a/an

    • A.

      Mordant

    • B.

      Accentuator

    • C.

      Accelerator

    • D.

      All of the above

    • E.

      B and C

    Correct Answer
    B. Accentuator
    Explanation
    Glacial HAc in hematoxylin acts as an accentuator. In staining techniques, accentuators are used to intensify the color of the dye or stain. In this case, glacial HAc enhances the staining properties of hematoxylin, which is commonly used in histology to stain cell nuclei. It helps to produce a more vivid and distinct staining of the target structures, aiding in their identification and analysis under a microscope.

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  • 15. 

    Association: DECOLORIZATION

    • A.

      Progressive staining

    • B.

      Regressive staining

    • C.

      Both

    • D.

      Neither

    Correct Answer
    B. Regressive staining
    Explanation
    Regressive staining is the correct answer because it refers to a staining technique where the excess stain is removed from the tissue, resulting in a lighter color. This process is commonly used in histology to highlight specific features of the tissue. Decolorization, which is mentioned in the association, is a key step in regressive staining. Progressive staining, on the other hand, involves intensifying the stain by leaving it on the tissue for a longer period.

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  • 16. 

    Association: Dye will not be the color seen in the tissue.

    • A.

      Progressive staining

    • B.

      Regressive staining

    • C.

      Both

    • D.

      Neither

    Correct Answer
    D. Neither
    Explanation
    In histology, both progressive and regressive staining methods result in the dye creating visible colors in the tissue. The association that dye will not be the color seen in the tissue does not align with these staining methods. Progressive staining involves controlled application to achieve the desired color, while regressive staining includes an additional step to remove excess dye. Both methods ultimately create a specific coloration in the tissue, making the association incorrect for these techniques.

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  • 17. 

    In ripening/oxidation of hematoxylin, an alkaline pH will oxidize it:

    • A.

      In a few hours

    • B.

      More slowly

    • C.

      More rapidly

    • D.

      None of the above

    Correct Answer
    C. More rapidly
    Explanation
    An alkaline pH will accelerate the ripening/oxidation process of hematoxylin, causing it to oxidize more rapidly. This means that the color change or staining effect produced by hematoxylin will occur at a faster rate when the pH is alkaline compared to other pH levels.

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  • 18. 

    Which will color the nuclues BLUE?(multiple answers)

    • A.

      Harris Hematoxylin

    • B.

      Wiegart's Hematoxylin

    • C.

      Ehrlich's Hematoxylin

    • D.

      Heidenhain's Hematoxylin

    • E.

      Delafield's Hematoxylin

    • F.

      Mallory's Hematoxylin

    Correct Answer(s)
    A. Harris Hematoxylin
    C. Ehrlich's Hematoxylin
    E. Delafield's Hematoxylin
    Explanation
    The correct answer is Harris Hematoxylin, Ehrlich's Hematoxylin, and Delafield's Hematoxylin. These three hematoxylins are commonly used in histology and pathology laboratories to stain the nucleus of cells blue. Hematoxylin is a natural dye that binds to DNA and RNA, and when combined with certain chemicals, it produces a blue color. Other hematoxylins listed in the question, such as Wiegart's Hematoxylin, Heidenhain's Hematoxylin, and Mallory's Hematoxylin, may produce different colors or have different staining properties.

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  • 19. 

    Which component substance in Harris Hematoxylin is not a mordant but increases staining instensity?

    • A.

      K alum

    • B.

      Mercuric oxide

    • C.

      95% ethanol

    • D.

      Glacial HAc

    • E.

      A and B

    Correct Answer
    D. Glacial HAc
    Explanation
    Glacial HAc, or glacial acetic acid, is the component substance in Harris Hematoxylin that is not a mordant but increases staining intensity. It acts as a solvent and helps to enhance the staining process by increasing the penetration of the dye into the tissue.

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  • 20. 

    Eosin is widely used as an aqueous or alcoholic solution with a concentration of:

    • A.

      0.1-0.5%

    • B.

      0.5-1%

    • C.

      1-2%

    • D.

      5-10%

    • E.

      70-90%

    Correct Answer
    B. 0.5-1%
    Explanation
    Eosin is commonly used as a stain in histology and microscopy. It is used to enhance the visibility of certain structures and tissues under a microscope. The concentration of eosin used is typically between 0.5-1%. This concentration range provides optimal staining results without causing excessive background staining or distortions in the tissue samples. Using a higher concentration could lead to oversaturation and loss of detail, while a lower concentration may not provide enough contrast for clear visualization. Therefore, a concentration of 0.5-1% is considered suitable for most staining purposes.

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  • 21. 

    For how long does the acidified counterstain, eosin, give a good result before its pH rises?

    • A.

      1 day

    • B.

      5 days

    • C.

      1 week

    • D.

      2 weeks

    • E.

      4 weeks

    Correct Answer
    D. 2 weeks
    Explanation
    Eosin is an acidified counterstain that is commonly used in histology and microscopy. It gives a good result before its pH rises, indicating that it remains effective for a certain period of time. The correct answer, 2 weeks, suggests that eosin can maintain its acidic properties and provide accurate staining for up to 2 weeks before its pH starts to increase. This means that it can be used reliably for staining purposes within this timeframe, ensuring accurate and consistent results.

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  • 22. 

    The commonly used kind of Eosin is: (multiple answers)

    • A.

      Eosin Y

    • B.

      Eosin B

    • C.

      Bluish

    • D.

      Yellowish

    Correct Answer(s)
    A. Eosin Y
    D. Yellowish
    Explanation
    Eosin is a commonly used dye in the field of histology and microscopy. It is available in different types, including eosin Y and eosin B. The question specifically asks for the commonly used kind of eosin, and the correct answer is eosin Y. Additionally, eosin Y is described as yellowish in color, which further supports its identification as the commonly used kind of eosin.

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  • 23. 

    The higher the concentration of the stain, the more the ionization

    • A.

      True

    • B.

      False

    Correct Answer
    A. True
    Explanation
    This statement is true because the concentration of a stain refers to the amount of the stain present in a given volume of solution. When the concentration of the stain is higher, there are more stain molecules available to interact with the solvent molecules. This increases the likelihood of ionization, which is the process of forming ions by gaining or losing electrons. Therefore, the higher the concentration of the stain, the more the ionization.

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  • 24. 

    The optimum pH is the pH at which each molecule has an equal number of (+) and (-) charges

    • A.

      True

    • B.

      False

    Correct Answer
    B. False
    Explanation
    The optimum pH refers to the specific pH level at which an enzyme or other biological molecule functions most effectively. It does not necessarily indicate that each molecule has an equal number of positive (+) and negative (-) charges. The term describing a pH where a molecule has an equal number of positive and negative charges is the isoelectric point (pI). At the isoelectric point, a molecule, such as a protein, has no net charge. This is different from the optimum pH, which is related to the conditions under which a molecule, like an enzyme, exhibits maximum activity or stability. Thus, the statement that the optimum pH is where each molecule has an equal number of positive and negative charges is incorrect. The correct term for that description is isoelectric point.

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  • 25. 

    Formalin fixative increases affinity of chemical compound for:

    • A.

      Acid stain

    • B.

      Basic stain

    • C.

      Both

    • D.

      Neither

    Correct Answer
    B. Basic stain
    Explanation
    Formalin fixative increases the affinity of chemical compounds for basic stains. This means that when tissues or cells are treated with formalin fixative, they become more likely to bind to basic dyes, resulting in a stronger and more visible staining. This is an important step in histology and pathology, as it helps to enhance the contrast and visibility of cellular structures under a microscope.

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  • 26. 

    TRUE for deparaffinization:

    • A.

      Makes stain permeate tissue section with highly organic solvents

    • B.

      1-2 changes of alcohol for 1 min

    • C.

      Uses 95% ehanol

    • D.

      All of the above

    • E.

      B and C only

    Correct Answer
    A. Makes stain permeate tissue section with highly organic solvents
    Explanation
    The correct answer is "all of the above." Deparaffinization is a process used in histology to remove paraffin wax from tissue sections before staining. This is done to allow the stain to penetrate the tissue effectively. The deparaffinization process typically involves multiple changes of alcohol, including the use of 95% ethanol, which is a highly organic solvent. Therefore, all of the given statements are true for deparaffinization.

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  • 27. 

    The most commonly used agent for removal of alcohol, making tissue transparent

    • A.

      Ethanol

    • B.

      Paraffin

    • C.

      Xylene

    • D.

      Acetone

    • E.

      H&E

    Correct Answer
    C. Xylene
    Explanation
    Xylene is commonly used as an agent for removing alcohol and making tissue transparent. It is a clear, colorless liquid that has the ability to dissolve alcohol and other substances, allowing for the dehydration and clearing of tissues. Xylene is frequently used in histology laboratories for processing tissue samples before embedding them in paraffin wax. It helps to remove any remaining water and alcohol from the tissues, making them transparent and ready for further processing and staining.

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  • 28. 

    This process serves to provide a clear visualization of tissue components studied using a medium with a Rf close to the slide and the tissue.

    • A.

      Deparaffinization

    • B.

      Hydration

    • C.

      Washing

    • D.

      Clearing

    • E.

      Mounting

    Correct Answer
    E. Mounting
    Explanation
    Mounting is the process of placing a tissue sample onto a microscope slide using a mounting medium. This medium is chosen to have a refractive index (Rf) similar to that of the slide and the tissue, which helps to minimize the distortion of the tissue components and provides a clear visualization. By mounting the tissue, it is securely attached to the slide and can be easily observed under a microscope.

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  • 29. 

    Von Apathy's gum syrup is used for

    • A.

      Permanent mounts

    • B.

      Temporary mounts

    • C.

      Both

    • D.

      Neither

    Correct Answer
    B. Temporary mounts
    Explanation
    von Apathy's gum syrup is a type of adhesive that is commonly used in microscopy for creating temporary mounts. Temporary mounts are prepared when the specimen is not required to be preserved for a long period of time. The gum syrup provides a temporary adhesive medium that allows the specimen to be easily mounted and observed under a microscope. It can be easily removed or dissolved when the observation is complete, making it suitable for temporary use.

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  • 30. 

    Blotting should be thouroughly done to dry up the section.

    • A.

      True

    • B.

      False

    Correct Answer
    B. False
    Explanation
    The statement suggests that blotting should be thoroughly done to dry up the section. However, the correct answer is False because blotting is not used to dry up a section. Blotting is a technique used to transfer substances from one surface to another, typically done to remove excess liquid or to separate components of a mixture.

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  • 31. 

    Clearing is done by:

    • A.

      10 dips in alcohol

    • B.

      Gradual run thru decreasing conc of alcohol

    • C.

      2 changes of xylol, 2 min each

    • D.

      2 or 3 changes of xylene for 1min

    • E.

      1-2 changes of absolute alcohol

    Correct Answer
    D. 2 or 3 changes of xylene for 1min
    Explanation
    The correct answer is 2 or 3 changes of xylene for 1 minute. This step is necessary in the clearing process to remove any remaining alcohol and prepare the specimen for embedding in a medium such as paraffin. Xylene is a common clearing agent used in histology to dehydrate and remove alcohol from tissue samples. Multiple changes of xylene ensure thorough clearing and optimal preservation of the tissue. The duration of 1 minute is typically sufficient for the xylene to effectively clear the specimen.

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  • 32. 

    Acid fuchsin is a counterstain that colors:

    • A.

      Magenta

    • B.

      Blue

    • C.

      Red

    • D.

      Orange

    • E.

      Red/purple

    Correct Answer
    A. Magenta
    Explanation
    Acid fuchsin is a counterstain that is known for coloring tissues and cells in a magenta shade. Counterstains are used in microscopy to provide contrast and enhance the visibility of certain structures or components. In this case, acid fuchsin is specifically chosen for its ability to produce a magenta color, which helps to differentiate and highlight specific elements in the sample being observed.

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  • 33. 

    Fixation treatment of tissues may cause:

    • A.

      Increased rates of penetration

    • B.

      Decreased rates of penetration

    • C.

      Both

    • D.

      Neither

    Correct Answer
    C. Both
    Explanation
    Fixation treatment of tissues can cause both increased and decreased rates of penetration. Fixation involves the use of chemicals to preserve and stabilize tissues for further analysis. Some fixation methods, such as formalin fixation, can cause tissue shrinkage and hardening, which can decrease the rate of penetration of subsequent reagents or stains. On the other hand, fixation can also increase the rate of penetration by improving tissue permeability and allowing better diffusion of reagents. Therefore, depending on the specific fixation method and conditions, both increased and decreased rates of penetration can occur.

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  • 34. 

    This process prevents rapid movement of fluid into and out of the tissue sections

    • A.

      Deparaffinization

    • B.

      Hyrdation

    • C.

      Washing

    • D.

      Dehydration

    • E.

      Clearing

    Correct Answer
    B. Hyrdation
    Explanation
    Hydration is the correct answer because it involves the addition of water or an aqueous solution to the tissue sections, which helps to restore their natural state and remove any dehydrating agents. This process prevents rapid movement of fluid into and out of the tissue sections, ensuring that the tissue remains hydrated and intact during subsequent steps of the procedure.

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  • 35. 

    An increase in temperature slows the rate of penetration

    • A.

      True

    • B.

      False

    Correct Answer
    B. False
    Explanation
    An increase in temperature actually speeds up the rate of penetration. This is because higher temperatures increase the kinetic energy of molecules, leading to faster diffusion and reaction rates. As a result, the molecules are able to move more quickly and penetrate more easily. Therefore, the statement that an increase in temperature slows the rate of penetration is incorrect.

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  • 36. 

    This process facilitates easy handling and storage

    • A.

      Deparaffinization

    • B.

      Hydration

    • C.

      Dehydration

    • D.

      Clearing

    • E.

      Mounting

    Correct Answer
    E. Mounting
    Explanation
    Mounting is the correct answer because it refers to the process of placing a specimen onto a slide and covering it with a coverslip. This step is essential for easy handling and storage of the specimen, as it protects it from damage and allows for easy examination under a microscope. The other options, such as deparaffinization, hydration, dehydration, and clearing, are all steps involved in the preparation of the specimen before mounting.

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  • 37. 

    TRUE for the mounting medium that is best for the Romanowsky stain (multiple answers)

    • A.

      Water insoluble

    • B.

      Temporary mount

    • C.

      Lquid paraffin

    • D.

      Synthetic resinous mounting medium

    • E.

      Slowly becomes acid because it oxidizes xylene

    Correct Answer(s)
    B. Temporary mount
    C. Lquid paraffin
    Explanation
    The Romanowsky stain is a type of stain commonly used in histology to stain blood cells. It requires a mounting medium that is water insoluble, as water-soluble mounting mediums would cause the stain to wash off. Temporary mount and liquid paraffin are both water insoluble mounting mediums that can be used with Romanowsky stain. Therefore, these two options are correct answers.

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  • 38. 

    Excess xylene favors bubbles on the slide

    • A.

      True

    • B.

      False

    Correct Answer
    A. True
    Explanation
    Excess xylene, a common solvent used in histology and microscopy, can cause the formation of bubbles on a slide. This is because xylene has a low surface tension, meaning it does not easily spread out and form a smooth layer on the slide. Instead, it tends to form small droplets that can trap air and create bubbles. Therefore, the statement "Excess xylene favors bubbles on the slide" is true.

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  • 39. 

    Optimum pH of Eosin

    • A.

      4.5-5

    • B.

      5.-8

    • C.

      6.3-7

    • D.

      5.4-5.6

    • E.

      None of the above

    Correct Answer
    D. 5.4-5.6
    Explanation
    The optimum pH of Eosin is 5.4-5.6. This means that Eosin exhibits its maximum effectiveness or activity at a pH range of 5.4-5.6. At this pH range, Eosin will have the highest solubility and will be most stable, resulting in the best performance for its intended purpose.

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  • 40. 

    If a drop of hematoxylin is added to water and the solution turns red brown, this means:

    • A.

      There is porportionate amount of hematoxylin and hematein

    • B.

      The stain is effective

    • C.

      It is completely hematein

    • D.

      It is ineffective

    • E.

      A and B

    • F.

      C and D

    Correct Answer
    E. A and B
    Explanation
    If a drop of hematoxylin is added to water and the solution turns red-brown, it means there is a disproportionate amount of hematoxylin and hematein. Hematoxylin is a natural dye that is extracted from the logwood tree and is commonly used in histology for staining nuclei in tissues. Hematein, on the other hand, is an oxidation product of hematoxylin. When hematoxylin is fresh and in its active form, it appears colorless to yellowish in solution. However, over time, hematoxylin oxidizes to form hematein, which imparts a red-brown color to the solution. A red-brown color indicates that the hematoxylin has undergone oxidation, resulting in a mixture of hematoxylin and hematein that is not in the ideal proportion for staining purposes. In this case, options A and B from the given choices would be correct, as they suggest that the stain is not effective and there is a disproportionate amount of hematoxylin and hematein present in the solution.

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  • 41. 

    Iron mordants stain the cytoplasm black

    • A.

      True

    • B.

      False

    Correct Answer
    B. False
    Explanation
    Iron mordants are used in histological staining, typically in conjunction with hematoxylin, to create a stable dye complex that primarily stains nuclei, giving them a black or dark blue color. The use of iron mordants in staining focuses on enhancing the dye's affinity for nucleic acids, like DNA and RNA, allowing for precise visualization of cell nuclei. However, iron mordants do not generally stain the cytoplasm black. The cytoplasm typically remains unstained or takes on a different color, depending on the staining protocol used. Therefore, the statement that iron mordants stain the cytoplasm black is incorrect; their primary function is to target nuclear material for staining.

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  • 42. 

    The mordant in Harris hematoxylin is K alum

    • A.

      True

    • B.

      False

    Correct Answer
    A. True
    Explanation
    Harris hematoxylin is a commonly used stain in histology and microscopy. A mordant is a substance that enhances the binding of a dye to the target tissue or structure. In the case of Harris hematoxylin, the mordant used is K alum, which helps to intensify the staining of nuclei. Therefore, the statement that the mordant in Harris hematoxylin is K alum is true.

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  • 43. 

    ASSOCIATION: congo red

    • A.

      Synthetic dye

    • B.

      Azo dye

    • C.

      Both

    • D.

      Neither

    Correct Answer
    C. Both
    Explanation
    Congo red is a synthetic dye that belongs to the class of azo dyes. Azo dyes are a type of synthetic dyes that contain one or more azo groups (-N=N-) in their chemical structure. Since Congo red is a synthetic dye and also an azo dye, the correct answer is "both".

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  • 44. 

    Artificial dyes are:

    • A.

      Coal tar dyes

    • B.

      Aniline dyes

    • C.

      Derived from hydrocarbon benzene

    • D.

      All of the above

    • E.

      A and C only

    Correct Answer
    D. All of the above
    Explanation
    Artificial dyes are classified as coal tar dyes, aniline dyes, and derived from the hydrocarbon benzene. This means that all of the options listed in the answer are correct. Artificial dyes can be obtained from coal tar, which is a byproduct of coal processing. Aniline dyes are synthetic dyes derived from aniline, a chemical compound derived from benzene. Therefore, artificial dyes can be both coal tar dyes and derived from the hydrocarbon benzene.

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  • 45. 

    When using Heidenhain's Iron hematoxylin, one should mix equal volumes of the mordant solution and stain solution

    • A.

      True

    • B.

      False

    Correct Answer
    B. False
    Explanation
    : When using Heidenhain's Iron Hematoxylin, the staining process typically involves separate applications of mordant and stain solution at different stages. It does not involve mixing equal volumes of the mordant solution and stain solution. Heidenhain's Iron Hematoxylin uses a specific staining protocol where the mordant is applied separately to prepare the tissue for staining, followed by the hematoxylin solution, which binds to the tissue through the mordant. Mixing the mordant and stain solutions could disrupt the staining process and lead to undesirable results. The key to Heidenhain's Iron Hematoxylin staining is the sequence and timing of applying the mordant and stain solutions to achieve precise and controlled staining, typically focusing on cell nuclei.

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  • 46. 

    ASSOCIATION: Kleermount

    • A.

      Synthetic resinous mounting medium

    • B.

      Natural resinous mounting medium

    • C.

      Both

    • D.

      Neither

    Correct Answer
    A. Synthetic resinous mounting medium
    Explanation
    The association "Kleermount" suggests a specific type of mounting medium used for a particular purpose. The term "synthetic resinous" indicates that the mounting medium is made from synthetic materials, specifically resins. This type of mounting medium is commonly used in laboratories and scientific settings for mounting specimens or slides. The term "natural resinous" refers to mounting mediums made from natural resins, which are less commonly used in scientific applications. Therefore, the correct answer is "synthetic resinous mounting medium" as it aligns with the association and its common usage.

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  • 47. 

    ASSOCIATION: Kaiser's glycerol jelly

    • A.

      Water soluble mounting medium

    • B.

      Aqueous mounting medium

    • C.

      Both

    • D.

      Neither

    Correct Answer
    C. Both
    Explanation
    Both water soluble mounting medium and aqueous mounting medium can be associated with Kaiser's glycerol jelly. This suggests that Kaiser's glycerol jelly can be used as a mounting medium that is both water soluble and aqueous.

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  • 48. 

    All of the following are natural dyes EXCEPT:

    • A.

      Saffron

    • B.

      Hematoxylin

    • C.

      Brazilin

    • D.

      Orecein

    • E.

      None of the above

    Correct Answer
    E. None of the above
    Explanation
    Saffron, hematoxylin, brazilin, and orecein are all examples of natural dyes used in various applications, including histology and textile dyeing.Saffron is derived from the saffron crocus and is used as a dye and spice.Hematoxylin is extracted from the heartwood of the logwood tree and is used in histological staining.Brazilin is derived from the Brazilwood tree and used as a natural dye.Orecein comes from lichens and is used as a natural dye in histology.Since all of these are natural dyes, the correct answer is none of the above. Each of these dyes is derived from natural sources and has applications in various fields, including histological staining and fabric dyeing.

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  • 49. 

    The staining done in Reticulocyte count is an example of:

    • A.

      Vital staining

    • B.

      Intravital staining

    • C.

      Supravitral staining

    • D.

      A and B

    • E.

      A and C

    Correct Answer
    E. A and C
    Explanation
    The staining done in Reticulocyte count is an example of vital staining because it involves staining living cells to observe their structure and function. It is also an example of supravital staining because it requires staining cells that have been removed from the body and are still viable.

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  • 50. 

    This non-mordant susbtance increases the rate of staining reaction but does not for a "lake"

    • A.

      Accentuator

    • B.

      Accelerator

    • C.

      Mordant

    • D.

      A and C

    • E.

      Band C

    Correct Answer
    B. Accelerator
    Explanation
    An accelerator is a non-mordant substance that increases the rate of staining reaction but does not form a "lake". This means that it helps to speed up the staining process without causing the dye to bind permanently to the material being stained, resulting in a "lake" formation.

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Quiz Review Timeline +

Our quizzes are rigorously reviewed, monitored and continuously updated by our expert board to maintain accuracy, relevance, and timeliness.

  • Current Version
  • Apr 24, 2024
    Quiz Edited by
    ProProfs Editorial Team
  • Jan 08, 2011
    Quiz Created by
    Outsider_matthew
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