Histopathologic Techniques: Routine Staining

55 Questions | By Outsider_matthew | Last updated: Jul 20, 2020 | Total Attempts: 5246

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Histopathologic Techniques: Routine Staining

A quiz on Ma'am G's lecture on Routine Staining for Third Year MedTech students of Velez College. Due to the random nature of her tests, this practice quiz is not meant to emulate the lecture exam. Rather, it serves merely as a reviewer. This tests consists of 55 items. Types of questions include: Multiple Choice, Association, Identification, simpleTrue or False, and modified multiple choice. Time limit is 60 minutes. Test questions are in random order.

Questions and Answers

1.

Which of the following are TRUE of staining? (multiple answers)
- A.
  
  Shows a hypochromic characteristic for neoplastic and malignant cells.
- B.
  
  It is the process that renders the different tissue components more visible through variation in color.
- C.
  
  Promotes easier optical dfferentiation.
- D.
  
  Sensitivity is the quality of sucessful staining which describes its ability to color one component and leaving others unstained.
- E.
  
  Accomplished by the use of dyes and stains.
2.

Principles of histological technique for distinction of tissue components are based on ____?
- A.
  
  Alteration of contrast
- B.
  
  Alteration of color
- C.
  
  Both
- D.
  
  Neither
3.

Dyes and Stains are:
- A.
  
  Essentially aromatic benzene ring components
- B.
  
  Possess the properties of color and ability to bind to tissue
- C.
  
  Both
- D.
  
  Neither
4.

What is the integral component of a dye that is responsible for altering light resonance?
- A.
  
  Chromophore
- B.
  
  Auxochrome
- C.
  
  Both
- D.
  
  Neither
5.

Cresyl fast violet is an example of
- A.
  
  Quinoid ring
- B.
  
  Quinone Imine Groups
- C.
  
  Both
- D.
  
  Neither
6.

Hematoxylin:
- A.
  
  Stains the nucleus
- B.
  
  Stains the cytoplasm
- C.
  
  Is negatively charged
- D.
  
  All of the above
- E.
  
  B and C only
7.

What is the heartwood from which hematoxylin comes from?
8.

What type of synthetic dye is EOSIN
- A.
  
  Azo dye
- B.
  
  Triphenylmethane
- C.
  
  Thiazine dye
- D.
  
  Xanthene dye
- E.
  
  Oxazine dye
9.

What type of synthetic dye is crystal violet?
- A.
  
  Azo dye
- B.
  
  Triphenylmethane
- C.
  
  Thiazine dye
- D.
  
  Xanthene dye
- E.
  
  Oxazine dye
10.

What type of dye is THIONINE?
- A.
  
  Azo dye
- B.
  
  Triphenylmethane
- C.
  
  Thiazine dye
- D.
  
  Xanthene dye
- E.
  
  Oxazine dye
11.

Janus green is a vitally stains:
- A.
  
  Nucleus
- B.
  
  Cytoplasm
- C.
  
  RES
- D.
  
  Mitochondria
- E.
  
  Chromatin
12.

Staining with a simple aqueous or alcoholic solution like Methylene Blue is an example of:
- A.
  
  Vital staining
- B.
  
  Routine staining
- C.
  
  Direct staining
- D.
  
  Indirect staining
- E.
  
  Progressive staining
- F.
  
  Regressive staining
13.

Iron hematoxylin:
- A.
  
  Enhances the combination of the dye with the nucleus
- B.
  
  Colors the nucleus black
- C.
  
  Used for progressive staining
- D.
  
  All of the above
- E.
  
  A and B
14.

Glacial HAc in hematoxylin is an example of a/an
- A.
  
  Mordant
- B.
  
  Accentuator
- C.
  
  Accelerator
- D.
  
  All of the above
- E.
  
  B and C
15.

Association: DECOLORIZATION
- A.
  
  Progressive staining
- B.
  
  Regressive staining
- C.
  
  Both
- D.
  
  Neither
16.

Association: Dye will not be the color seen in the tissue
- A.
  
  Progressive staining
- B.
  
  Regressive staining
- C.
  
  Both
- D.
  
  Neither
17.

In ripening/oxidation of hematoxylin, an alkaline pH will oxidize it:
- A.
  
  In a few hours
- B.
  
  More slowly
- C.
  
  More rapidly
- D.
  
  None of the above
18.

Which will color the nuclues BLUE?(multiple answers)
- A.
  
  Harris Hematoxylin
- B.
  
  Wiegart's Hematoxylin
- C.
  
  Ehrlich's Hematoxylin
- D.
  
  Heidenhain's Hematoxylin
- E.
  
  Delafield's Hematoxylin
- F.
  
  Mallory's Hematoxylin
19.

Which component substance in Harris Hematoxylin is not a mordant but increases staining instensity?
- A.
  
  K alum
- B.
  
  Mercuric oxide
- C.
  
  95% ethanol
- D.
  
  Glacial HAc
- E.
  
  A and B
20.

Eosin is widely used as an aqueous or alcoholic solution with a concentration of:
- A.
  
  0.1-0.5%
- B.
  
  0.5-1%
- C.
  
  1-2%
- D.
  
  5-10%
- E.
  
  70-90%
21.

For how long does the acidified counterstain, eosin, give a good result before its pH rises?
- A.
  
  1 day
- B.
  
  5 days
- C.
  
  1 week
- D.
  
  2 weeks
- E.
  
  4 weeks
22.

The commonly used kind of Eosin is: (multiple answers)
- A.
  
  Eosin Y
- B.
  
  Eosin B
- C.
  
  Bluish
- D.
  
  Yellowish
23.

The higher the concentration of the stain, the more the ionization
- A.
  
  True
- B.
  
  False
24.

The optimum pH is the pH at which each molecule has an equal number of (+) and (-) charges
- A.
  
  True
- B.
  
  False
25.

Formalin fixative increases affinity of chemical compound for:
- A.
  
  Acid stain
- B.
  
  Basic stain
- C.
  
  Both
- D.
  
  Neither