Histology MCQ
460 Questions
| Attempts: 2263
Questions and Answers
- 1.
When using a compound microscope, objective lenses can be found to have a magnification of all of the following, EXCEPT?
- A.
4X
- B.
10X
- C.
40X
- D.
100X
- E.
1000X
Correct Answer
E. 1000XExplanation
A compound microscope has two lenses: an eyepiece lens and objective lens. The eyepiece lens usually has a magnification of 10X. There are objective lenses on the revolving nosepiece with varying magnifications. Most compound microscopes have objective lenses with magnification of 4X, 10X, and 40X. Some compound microscopes also have an oil immersion lens with a magnification of 100X.Rate this question:
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- 2.
What is "compound microscope"?
- A.
Microscope with the capability to view oil immersion
- B.
Microscope with the capability to view compounds
- C.
Microscope with a single lens
- D.
Microscope with two lenses
- E.
Microscope with three lenses
Correct Answer
D. Microscope with two lensesExplanation
A compound microscope has two lenses: an eyepiece lens and objective lens. The eyepiece lens usually has a magnification of 10X. There are objective lenses on the revolving nosepiece with varying magnifications. Most compound microscopes have objective lenses with magnification of 4X, 10X, and 40X. Some compound microscopes also have an oil immersion lens with a magnification of 100X.Rate this question:
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- 3.
What is the total magnification achieved with a compound microscope?
- A.
Magnification of objective lens
- B.
Magnification of ocular lens
- C.
Magnification of ocular lens added to the magnification of the objective lens
- D.
Magnification of ocular lens multiplied by the magnification of the objective
- E.
Magnification of condenser lens multiplied by the magnification of the objective lens
Correct Answer
D. Magnification of ocular lens multiplied by the magnification of the objectiveExplanation
To calculate the total magnification achieved with a compound microscope, the magnification of the ocular lens is multiplied by the magnification of the objective lens. For example, if viewing a sample with the 40x objective, the total magnification would be calculated as follows: a 10X ocular lens used with a 40X objective lens, the total magnification is 400X (10 x 40).Rate this question:
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- 4.
What is the maximum resolving power seen with a compound microscope?
- A.
2 millimeters
- B.
2 micrometers
- C.
2 angstroms
Correct Answer
B. 2 micrometersExplanation
Resolving power is the ability to see two things as discrete images. With normal vision, there is a resolving power of about of 100 micrometers. A compound microscope has a resolving power of approximately .2 micrometers. In other words, two marks .2 micrometers apart can be seen as two distinct entities. Any closer than this, they are perceived as one object.Rate this question:
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- 5.
What is the turret?
- A.
Base
- B.
Nosepiece
- C.
Stage
- D.
Tube
- E.
Diaphragm
Correct Answer
B. NosepieceExplanation
The base is the bottom of the microscope. The revolving nosepiece is also called a turret. The objective lens are attached to the nosepiece (or turret). The slide rests on the stage. The tube is the structure which connects the eyepiece to the objective lenses (it is shaped like a tube; thus, its name). The diaphragm controls the diameter of the cone of light.Rate this question:
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- 6.
On a microscope, what structure connects the eyepiece to the objective lens?
- A.
Base
- B.
Nosepiece
- C.
Stage
- D.
Tube
- E.
Diaphragm
Correct Answer
D. TubeExplanation
The base is the bottom of the microscope. The revolving nosepiece is also called a turret. The objective lens are attached to the nosepiece (or turret). The slide rests on the stage. The tube is the structure which connects the eyepiece to the objective lenses (it is shaped like a tube; thus, its name). The diaphragm controls the diameter of the cone of light.Rate this question:
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- 7.
In a good compound microscope, the focus knob does not have to be readjusted when changing the magnification. What is this phenomenon called?
- A.
Parfocal
- B.
Unifocal
- C.
Bifocal
- D.
Focused
- E.
Convergent
Correct Answer
A. ParfocalExplanation
Parafocal is the term used for a microscope if the focus knob does not have to be readjusted when changing the magnifications. This phenomenon is seen with good compound microscopes. In other words, when the specimen is in focus at 4X and the objective is switched to 10X, the specimen remains in focus.Rate this question:
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- 8.
What is another name for the light microscope?
- A.
Simple microscope
- B.
Compound microscope
- C.
Phase contrast microscope
- D.
Dissection microscope
- E.
Transmission electron microscope
Correct Answer
B. Compound microscopeExplanation
A simple microscope has only one lens. A compound microscope utilizes two lenses: an ocular lens and an objective lens. The compound microscope is also referred to as a "light microscope" or "bright field microscope". A phase contrast microscope is useful for examining living cells, because the specimen does not need to be stained. A dissection microscope uses low power magnification. Things appear three dimensional with a dissection microscope. A transmission electron microscope does not use light, but rather a beam of electronsRate this question:
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- 9.
Which microscope does not rely on visible light?
- A.
Simple microscope
- B.
Compound microscope
- C.
Phase contrast microscope
- D.
Dissection microscope
- E.
Transmission electron microscope
Correct Answer
E. Transmission electron microscopeExplanation
A simple microscope has only one lens. A compound microscope utilizes two lenses: an ocular lens and an objective lens. The compound microscope is also referred to as a "light microscope" or "bright field microscope". A phase contrast microscope is useful for examining living cells, because the specimen does not need to be stained. A dissection microscope uses low power magnification. Things appear three dimensional with a dissection microscope. A transmission electron microscope does not use light, but rather a beam of electrons.Rate this question:
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- 10.
Which microscope makes things appear three dimensional?
- A.
Simple microscope
- B.
Compound microscope
- C.
Phase contrast microscope
- D.
Dissection microscope
- E.
Transmission electron microscope
Correct Answer
D. Dissection microscopeExplanation
A simple microscope has only one lens. A compound microscope utilizes two lenses: an ocular lens and an objective lens. The compound microscope is also referred to as a "light microscope" or "bright field microscope". A phase contrast microscope is useful for examining living cells, because the specimen does not need to be stained. A dissection microscope uses low power magnification. Things appear three dimensional with a dissection microscope. A transmission electron microscope does not use light, but rather a beam of electrons.Rate this question:
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- 11.
When using a compound microscope, what is the magnification of the oil immersion lens?
- A.
4X
- B.
10X
- C.
40X
- D.
100X
- E.
1000X
Correct Answer
D. 100XExplanation
A compound microscope has two lenses: an eyepiece lens and objective lens. The eyepiece lens usually has a magnification of 10X. There are objective lenses on the revolving nosepiece with varying magnifications. Most compound microscopes have objective lenses with magnification of 4X, 10X, and 40X. Some compound microscopes also have an oil immersion lens with a magnification of 100X.Rate this question:
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- 12.
What is the usual magnification of the ocular lens on a compound microscope?
- A.
1X
- B.
10X
- C.
100X
- D.
1000X
- E.
10,000 X
Correct Answer
B. 10XExplanation
The usual magnification of an ocular lens on a compound microscope is 10X. Some microscopes have a 15X eyepiece lens. The ocular lens is the lens at the top of the tube, the one that you first look through when using a microscope. It is also called the eyepiece lens.Rate this question:
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- 13.
When using oil immersion to view a tissue, what is the refractive index of the oil?
- A.
Zero
- B.
Same as air
- C.
Same as glass
- D.
Same as water
- E.
None of the above
Correct Answer
C. Same as glassExplanation
With light microscopy, there normally is a space of air between the slide and the lens. Oil immersion replaces that space of air with oil. The refractive index of the oil is the same as glassRate this question:
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- 14.
What is the role of the condenser lens?
- A.
Control the aperture of light
- B.
Increase the magnification
- C.
Focus the light on the specimen
- D.
Initial magnification of 10X
- E.
Provide light
Correct Answer
C. Focus the light on the specimenExplanation
The role of the condenser lens is to focus light on the specimen. It is used with higher magnifications.Rate this question:
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- 15.
On a microscope, what structure varies the diameter of the cone of light?
- A.
Base
- B.
Nosepiece
- C.
Stage
- D.
Tube
- E.
Diaphragm
Correct Answer
E. DiaphragmExplanation
The base is the bottom of the microscope. The revolving nosepiece is also called a turret. The objective lens are attached to the nosepiece (or turret). The slide rests on the stage. The tube is the structure which connects the eyepiece to the objective lenses (it is shaped like a tube; thus, its name). The diaphragm controls the diameter of the cone of light.Rate this question:
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- 16.
Where do you place the slide when using a microscope?
- A.
Base
- B.
Nosepiece
- C.
Stage
- D.
Tube
- E.
Diaphragm
Correct Answer
C. StageExplanation
The base is the bottom of the microscope. The revolving nosepiece is also called a turret. The objective lens are attached to the nosepiece (or turret). The slide rests on the stage. The tube is the structure which connects the eyepiece to the objective lenses (it is shaped like a tube; thus, its name). The diaphragm controls the diameter of the cone of light.Rate this question:
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- 17.
What is the bottom of a microscope called?
- A.
Base
- B.
Nosepiece
- C.
Stage
- D.
Tube
- E.
Diaphragm
Correct Answer
A. BaseExplanation
The base is the bottom of the microscope. The revolving nosepiece is also called a turret. The objective lens are attached to the nosepiece (or turret). The slide rests on the stage. The tube is the structure which connects the eyepiece to the objective lenses (it is shaped like a tube; thus, its name). The diaphragm controls the diameter of the cone of light.Rate this question:
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- 18.
What is another name for the bright field microscope?
- A.
Simple microscope
- B.
Compound microscope
- C.
Phase contrast microscope
- D.
Dissection microscope
- E.
Transmission electron microscope
Correct Answer
B. Compound microscopeExplanation
A simple microscope has only one lens. A compound microscope utilizes two lenses: an ocular lens and an objective lens. The compound microscope is also referred to as a "light microscope" or "bright field microscope". A phase contrast microscope is useful for examining living cells, because the specimen does not need to be stained. A dissection microscope uses low power magnification. Things appear three dimensional with a dissection microscope. A transmission electron microscope does not use light, but rather a beam of electrons.Rate this question:
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- 19.
Which microscope would be particularly useful for looking at living cells?
- A.
Simple microscope
- B.
Compound microscope
- C.
Phase contrast microscope
- D.
Dissection microscope
- E.
Transmission electron microscope
Correct Answer
C. Phase contrast microscopeExplanation
A simple microscope has only one lens. A compound microscope utilizes two lenses: an ocular lens and an objective lens. The compound microscope is also referred to as a "light microscope" or "bright field microscope". A phase contrast microscope is useful for examining living cells, because the specimen does not need to be stained. A dissection microscope uses low power magnification. Things appear three dimensional with a dissection microscope. A transmission electron microscope does not use light, but rather a beam of electrons.Rate this question:
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- 20.
Which type of microscope has only one lens?
- A.
Simple microscope
- B.
Compound microscope
- C.
Phase contrast microscope
- D.
Dissection microscope
- E.
Transmission electron microscope
Correct Answer
A. Simple microscopeExplanation
A simple microscope has only one lens. A compound microscope utilizes two lenses: an ocular lens and an objective lens. The compound microscope is also referred to as a "light microscope" or "bright field microscope". A phase contrast microscope is useful for examining living cells, because the specimen does not need to be stained. A dissection microscope uses low power magnification. Things appear three dimensional with a dissection microscope. A transmission electron microscope does not use light, but rather a beam of electrons.Rate this question:
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- 21.
Which of the following stain blue with H&E stain?
- A.
Cytoplasm
- B.
Collagen fibers
- C.
Nucleus
- D.
Elastic fibers
- E.
Decalcified bone matrix
Correct Answer
C. NucleusExplanation
"H&E" stands for hematoxylin and eosin. Hematoxylin can be thought of as a basic dye. It binds to acidic structures, staining them blue to purple. For example, it will bind and stain nucleic acids. Therefore, the nucleus stains blue.Rate this question:
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- 22.
What are Sudan stains used primarily for?
- A.
Blood
- B.
Fat
- C.
Nervous tissue
- D.
Elastic fibers
- E.
Decalcified bone matrix
Correct Answer
B. FatExplanation
Sudan stains are used for staining of lipids and phospholipids. Examples of such stains are sudan black and sudan IV. Oil red O is another stain used for demonstrating fat.Rate this question:
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- 23.
What is Mucicarmine stain used primarily for?
- A.
Blood
- B.
Fat
- C.
Nervous tissue
- D.
Elastic fibers
- E.
Epithelial mucin
Correct Answer
E. Epithelial mucinExplanation
Mucicarmine is used to see epithelial mucin. It stains it a deep red.Rate this question:
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- 24.
What is Wright's stain used primarily for?
- A.
Blood
- B.
Fat
- C.
Nervous tissue
- D.
Elastic fibers
- E.
Decalcified bone matrix
Correct Answer
A. BloodExplanation
Wright's stain is used for blood smears and bone marrow. Red blood cells will stain pink.Rate this question:
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- 25.
What color do elastic fibers stain with Verhoeff Elastic stain?
- A.
Red/Orange
- B.
Pink/red
- C.
Purple/Red
- D.
Blue/black
- E.
Green/blue
Correct Answer
D. Blue/blackExplanation
Verhoeff Elastic stain stains elastic fibers blue/black. Collagen stains pink/red.Rate this question:
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- 26.
During the preparation of a routine H&E slide, what step occurs after the tissue is preserved?
- A.
Fixation
- B.
Embedding in paraffin
- C.
Staining
- D.
Slicing
- E.
Dehydration
Correct Answer
E. Dehydration -
- 27.
During the preparation of a routine H&E slide, how is the tissue preserved?
- A.
Fixation
- B.
Embedding in paraffin
- C.
Staining
- D.
Slicing
- E.
Dehydration
Correct Answer
A. Fixation -
- 28.
During the preparation of a routine H&E slide, what allows the tissue to be visualized?
- A.
Fixation
- B.
Embedding in paraffin
- C.
Staining
- D.
Slicing
- E.
Dehydration
Correct Answer
C. StainingExplanation
There are several steps in the preparation of a slide for microscopic examination. First, the tissue is "fixed" with formalin. This preserves it. After the tissue is fixed, it is dehydrated (dehydration will occur twice during slide preparation). The tissue sample is then embedded in paraffin. This forms a solid block, so that it can be placed in a microtome. It is then thinly sliced using a microtome. A thin slice of the specimen is then placed on a slide. The paraffin is removed by dissolving it with a chemical. The tissue is then rehydrated. In order to view the tissue, it is stained. After it is stained, it is again dehydrated. A coverslip is the mounted on top of it. The specimen is then ready for viewing.Rate this question:
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- 29.
Which of the following would be best suited to visualize reticular fibers?
- A.
Wright's stain
- B.
Hematoxylin and eosin stain
- C.
Sudan stain
- D.
Silver impregnation
- E.
Masson's trichrome stain
Correct Answer
D. Silver impregnation -
- 30.
Which of the following would be best suited to visualize lipid?
- A.
Wright's stain
- B.
Hematoxylin and eosin stain
- C.
Sudan stain
- D.
Silver impregnation
- E.
Masson's trichrome stain
Correct Answer
C. Sudan stain -
- 31.
During the preparation of a routine H&E slide, what allows the tissue to hold its form?
- A.
Fixation
- B.
Embedding in paraffin
- C.
Staining
- D.
Slicing
- E.
Dehydration
Correct Answer
B. Embedding in paraffin -
- 32.
During the preparation of a routine H&E slide, what step occurs after the tissue is embedded in paraffin?
- A.
Fixation
- B.
Rehydration
- C.
Staining
- D.
Slicing
- E.
Dehydration
Correct Answer
D. Slicing -
- 33.
During the preparation of a routine H&E slide, what step occurs after the tissue is stained?
- A.
Fixation
- B.
Embedding in paraffin
- C.
Staining
- D.
Slicing
- E.
Dehydration
Correct Answer
E. DehydrationExplanation
There are several steps in the preparation of a slide for microscopic examination. First, the tissue is "fixed" with formalin. This preserves it. After the tissue is fixed, it is dehydrated (dehydration will occur twice during slide preparation). The tissue sample is then embedded in paraffin. This forms a solid block, so that it can be placed in a microtome. It is then thinly sliced using a microtome. A thin slice of the specimen is then placed on a slide. The paraffin is removed by dissolving it with a chemical. The tissue is then rehydrated. In order to view the tissue, it is stained. After it is stained, it is again dehydrated. A coverslip is then mounted on top of it. The specimen is then ready for viewing.Rate this question:
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- 34.
Which of the following is an acid dye?
- A.
Azure II
- B.
Toluidine blue
- C.
Methylene blue
- D.
Eosin
- E.
Thionine
Correct Answer
D. EosinExplanation
Eosin is an acid dye. It will bind to and stain basic structures (or negatively charged structures), such as cationic amino groups on proteins. It stains them pink. Cytoplasm, muscle, connective tissue, and decalcified bone matrix all stain pink with eosin. "H&E" stand for hematoxylin and eosin. Hematoxylin and eosin stain is used for routine tissue preparation frequently.
Azure II, Toluidine blue, Methylene blue, Thionine are all basic dyes.Rate this question:
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- 35.
Which of the following would be best suited for peripheral blood smears?
- A.
Wright's stain
- B.
Hematoxylin and eosin stain
- C.
Sudan stain
- D.
Silver impregnation
- E.
Masson's trichrome stain
Correct Answer
A. Wright's stain -
- 36.
Which of the following stains is used for routine histological examination?
- A.
Wright's stain
- B.
Hematoxylin and eosin stain
- C.
Sudan stain
- D.
Silver impregnation
- E.
Masson's trichrome stain
Correct Answer
B. Hematoxylin and eosin stainExplanation
A peripheral blood smear would be best visualized with Wright's stain. Hematoxylin and eosin stain is the most commonly used tissue stain for routine histological examination. Lipids are best displayed with a sudan stain. Silver impregnation, such as with a reticular stain, can be used to visualize reticular fibers. Collagen fibers can be differentiated from other fibers by staining with Masson's trichrome stain.Rate this question:
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- 37.
What is Prussian blue stain used for?
- A.
Sodium
- B.
Potassium
- C.
Calcium
- D.
Copper
- E.
Iron
Correct Answer
E. IronExplanation
Prussian blue stain is used to stain iron.Rate this question:
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- 38.
What color does hematoxylin stain structures?
- A.
Orange
- B.
Blue
- C.
Pink
- D.
Red
- E.
Green
Correct Answer
B. BlueExplanation
"H & E" stands for hematoxylin and eosin. Hematoxylin can be thought of as a basic dye. It binds to acidic structures, staining them blue to purple. For example, it will bind and stains nucleic acids. Therefore, the nucleus stains blue.Rate this question:
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- 39.
Which of the following would be best suited to differentiate collagen fibers from other fibers ?
- A.
Wright's stain
- B.
Hematoxylin and eosin stain
- C.
Sudan stain
- D.
Silver impregnation
- E.
Masson's trichrome stain
Correct Answer
E. Masson's trichrome stainExplanation
A peripheral blood smear would be best visualized with Wright's stain. Hematoxylin and eosin stain is the most commonly used tissue stain for routine histological examination. Lipids are best displayed with a sudan stain. Silver impregnation, such as with a reticular stain, can be used to visualize reticular fibers. Collagen fibers can be differentiated from other fibers by staining with Masson's trichrome stain.Rate this question:
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- 40.
Which of the following is NOT a membranous organelle?
- A.
Lysosomes
- B.
Peroxisomes
- C.
Mitochondria
- D.
Ribosomes
- E.
Endoplasmic reticulum
Correct Answer
D. RibosomesExplanation
Lysosomes, peroxisomes, mitochondria, and endoplasmic reticulum are all membranous organelles.Rate this question:
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- 41.
Which organelle contains digestive enzymes?
- A.
Rough endoplasmic reticulum
- B.
Smooth endoplasmic reticulum
- C.
Lysosome
- D.
Golgi apparatus
- E.
Mitochondria
Correct Answer
C. LysosomeExplanation
Lysosomes are membrane bound sacs of digestive enzymes. They are nicknamed "suicide sacs".Rate this question:
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- 42.
Which organelle is considered the powerhouse of the cell?
- A.
Rough endoplasmic reticulum
- B.
Smooth endoplasmic reticulum
- C.
Lysosome
- D.
Golgi apparatus
- E.
Mitochondria
Correct Answer
E. MitochondriaExplanation
The mitochondria is the site of ATP production for the cell. ATP is the energy currency of the cell. Mitochondria are nicknamed, the "powerhouse".Rate this question:
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- 43.
What structure is at the base of cilia?
- A.
Ribosomes
- B.
Peroxisome
- C.
Microfilaments
- D.
Centrioles
- E.
Nucleoli
Correct Answer
D. CentriolesExplanation
Centrioles are composed of microtubules. Centrioles are at the base of cilia and flagella. They are also involved with cell division.Rate this question:
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- 44.
Which of the following is NOT considered an inclusion?
- A.
Pigment
- B.
Glycogen
- C.
Lipid
- D.
Secretory granules
- E.
Mitochondria
Correct Answer
E. MitochondriaExplanation
Mitochondria is an organelle. Pigment, glycogen, lipid, and secretory granules are all considered inclusions. Organelles perform an activity; they have a job; they require energy. Inclusions are for storage. Inclusions do not perform an energy requiring job.Rate this question:
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- 45.
Collectively, what is the cytoplasm and nucleus called?
- A.
Plasmalemma
- B.
Glycocalyx
- C.
Protoplasm
- D.
Cristae
- E.
Ground substance
Correct Answer
C. ProtoplasmExplanation
The cell boundary is defined by the cell membrane or plasmalemma. Glycocalyx is a polysacchride that can be found on the outer surface of the cell membranes. Collectively, the cytoplasm and nucleus form the protoplasm of a cell. The folds on the inner mitochodrial membrane are called cristae. The cytoplasm that surrounds organelles is the cytoplasmic ground substance or cytosol.Rate this question:
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- 46.
How thick is the plasma membrane?
- A.
8-10 angstroms
- B.
8-10 nanometers
- C.
8-10 micrometers
- D.
8-10 millimeters
- E.
None of the above
Correct Answer
B. 8-10 nanometersExplanation
The plasma membrane is 8-10 nanometers thick.Rate this question:
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- 47.
What is the term for the general process that cells us to expunge material from the cell?
- A.
Endocytosis
- B.
Exocytosis
- C.
Pinocytosis
- D.
Phagocytosis
- E.
Active transport
Correct Answer
B. ExocytosisExplanation
Endotyosis is the term for the process cells use to have substances enter them. Exoctyosis is the term for the process cells use to remove substances. Phagocytosis is "cell eating" and is used for the transport of particulate matter. Pinocytosis is "cell drinking". Active transport is a method which requires energy.Rate this question:
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- 48.
Which organelle sorts and packages proteins within a cell?
- A.
Rough endoplasmic reticulum
- B.
Smooth endoplasmic reticulum
- C.
Lysosome
- D.
Golgi apparatus
- E.
Mitochondria
Correct Answer
D. Golgi apparatusExplanation
The Golgi apparatus are stacks of membranous sacs. Proteins are sorted and packaged in the Golgi apparatus.Rate this question:
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- 49.
Which of the following is NOT a membranous organelle?
- A.
Lysosomes
- B.
Filaments
- C.
Peroxisomes
- D.
Mitochondria
- E.
Endoplasmic reticulum
Correct Answer
B. FilamentsExplanation
Lysosomes, peroxisomes, mitochondria, and endoplasmic reticulum are all membranous organelles.Rate this question:
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- 50.
What are the folds on the inner mitochodrial membrane called?
- A.
Plasmalemma
- B.
Glycocalyx
- C.
Protoplasm
- D.
Cristae
- E.
Ground substance
Correct Answer
D. CristaeExplanation
The cell boundary is defined by the cell membrane or plasmalemma. Glycocalyx is a polysacchride that can be found on the outer surface of the cell membranes. Collectively, the cytoplasm and nucleus form the protoplasm of a cell. The folds on the inner mitochodrial membrane are called cristae. The cytoplasm that surrounds organelles is the cytoplasmic ground substance or cytosol.Rate this question:
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Mar 21, 2022Quiz Edited by
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Feb 28, 2016Quiz Created by
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