Histological Techniques 2

25 Questions | Total Attempts: 1148

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Histological Techniques 2 - Quiz

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Questions and Answers
  • 1. 
    A lipid is stained using Sudan III or Sudan IV What is true ?
    • A. 

      Nuclei are counterstained with haematoxylin

    • B. 

      Nuclei are counterstained with Nuclear red

    • C. 

      Nuclei appears red

    • D. 

      Nuclei appears dark

    • E. 

      Lipids appear red

    • F. 

      Lipids appear yellow

  • 2. 
    A neutral lipid is stained with Sudan III What color does it appear ?
    • A. 

      Yellow

    • B. 

      Pink

    • C. 

      Red

    • D. 

      Dark

    • E. 

      Blue

  • 3. 
    Elastin is stained with which method ?
    • A. 

      Sudan III

    • B. 

      Auric chloride

    • C. 

      PerlĀ“s reaction

    • D. 

      Aniline blue

    • E. 

      Weigert resorcin-fuchsin technique

  • 4. 
    Elastin is counterstained with ? and appearing in which color(choose the 1 true)
    • A. 

      Haemotoxylin

    • B. 

      Eosin

    • C. 

      Dark

    • D. 

      Nuclear red

    • E. 

      Mayers mucicarmine

    • F. 

      Red

  • 5. 
    Reticular fibers are stained using which methods ?
    • A. 

      Nuclear red

    • B. 

      Gomoroi ammoniac silver nitrate

    • C. 

      Luxol blue

    • D. 

      Haemotoxylin

    • E. 

      Eosin

  • 6. 
    Blue trichrome is used to stain ?
    • A. 

      Glycogen

    • B. 

      Mucus

    • C. 

      Myelin sheats

    • D. 

      Acidic tissue structures

    • E. 

      Basic tissue structures

    • F. 

      Collagen

  • 7. 
    In the following picture, the connective tissue is stained using what staining method ?
    • A. 

      Colloid iron

    • B. 

      Perls reaction

    • C. 

      Luxol blue

    • D. 

      Blue trichrome

  • 8. 
    What is the first step before staining the sections ?
    • A. 

      Heat it up and place it in paraffin at 56c

    • B. 

      Rins the section out in distilled water

    • C. 

      The section must be deparaffinized in Xylene

    • D. 

      Freezing it

  • 9. 
    What is true for basic stains ?
    • A. 

      They bind to cytoplasm

    • B. 

      They bind to basic structures

    • C. 

      They bind to ergastoplasm

    • D. 

      They bind to ground substance of cartilage

    • E. 

      They bind to mucus

    • F. 

      They bind to acidic tissue structures

  • 10. 
    What is true about the staining method of Haemotoxylin and eosin ?
    • A. 

      Haemotoxylin stains nuclei Brown

    • B. 

      Eosin stains nuclei pink

    • C. 

      Haemotoxylin stains nuclei Dark

    • D. 

      Eosin stains structures pink

    • E. 

      Eosin stains structures red

    • F. 

      Haemotoxylin stains structures blue

    • G. 

      Eosin stains granules of eosinophilic leukocytes intensively red

  • 11. 
    Which of the following are stained intensively red by Eosin ?
    • A. 

      Erythrocytes

    • B. 

      Structures

    • C. 

      Nuclei

    • D. 

      Acidophilic cells

    • E. 

      Eosin doesnt stains red

  • 12. 
    Acidic stains bind to:
    • A. 

      Chromatin of the nucleus

    • B. 

      Mucus

    • C. 

      Ergastoplasm

    • D. 

      Ground substance of cartilage

  • 13. 
    When sections have been stained and are prepared for covering What must be done ?
    • A. 

      Stained sections most be dehydrated with ethanol

    • B. 

      Stained sections most be rehydrated with ethanol

    • C. 

      Stained sections most be dehydrated with xylene

    • D. 

      Stained sections most De-paraffinized using xylene

    • E. 

      Stained sections most be embedded in paraffin

    • F. 

      Stained sections are mounted in Canada balsam

  • 14. 
    In flourescent microscopy a sample is exposed to:
    • A. 

      Light of long wavelength

    • B. 

      Light of short wavelength

    • C. 

      Light of normal wavelength

  • 15. 
    In flourescent microscopy tissue reflects light of short wavelength back as longer wavelengths. What most the tissue contain for this to happen ?
    • A. 

      Natriumflourid

    • B. 

      Fluor

    • C. 

      Fluorochrome

  • 16. 
    To visualize structures that do not exhibit primary fluorescence, fluorescent dyes (fluorochromes) must be utilized; light emitted by these dyes is referred to as
    • A. 

      Quaternary fluorescence

    • B. 

      Primary fluorescence

    • C. 

      Secondary flourescence

  • 17. 
    Which are nuclear dyes of the following in case of Fluorescence microscopy ?
    • A. 

      Rhodamine 123

    • B. 

      DiI

    • C. 

      Acridine orange

    • D. 

      Propidium iodide;

  • 18. 
    Perls‘ reaction are used for ?
    • A. 

      Staining of glycogen

    • B. 

      Detection of Fe3+

    • C. 

      Staining of mucus

    • D. 

      Detecting phospholipides in myelin sheath

    • E. 

      Detecting Haemosiderin

    • F. 

      Staining nuclei

  • 19. 
    What method is best for detection of glycogene ? and what is the color of the stain ?
    • A. 

      Red

    • B. 

      Best carmine

    • C. 

      Blue

    • D. 

      Mayers mucicarmine

    • E. 

      Purple

    • F. 

      Perls reaction

    • G. 

      Pas reaction

  • 20. 
    What method is used for detection of  acidic mucopolysaccharides ?
    • A. 

      Mayers mucicarmine

    • B. 

      Colloid iron according to Hale-Muller

    • C. 

      Pas method

    • D. 

      Nuclear red

    • E. 

      Haemotoxylin

  • 21. 
    What is true when staining Mucus ?
    • A. 

      The stain becomes purple

    • B. 

      The counterstain is Haematoxylin

    • C. 

      The counterstain is Nuclear red

    • D. 

      The mucus stains pink

    • E. 

      The stain used is Best carmine

    • F. 

      The stain used is Mayers Mucicarmine

  • 22. 
    What is true for staining pigment Melanin ?
    • A. 

      Weigert resorcin-fuchsin technique is used

    • B. 

      Green trichrome is used as an counterstain

    • C. 

      Van gieson stain is used

    • D. 

      The counterstain is Hematoxylin

    • E. 

      Argentaffin reaction is used

  • 23. 
    What of the following are true ?
    • A. 

      Haematoxylin and eosin are nucleic stains

    • B. 

      Haemotoyxlin and eosin are bind to basic structures

    • C. 

      Argentaffin reaction stains structures black

    • D. 

      Myelin sheats are counterstained with nuclear red

    • E. 

      Argentaffin reaction stains Polysaccharides

    • F. 

      Green trichrome are counterstained with Haemotoxylin

  • 24. 
    What one is the one true method for detection of DNA ?
    • A. 

      Hale-muller

    • B. 

      Gomori

    • C. 

      Feulgens nuclear reaction reaction

    • D. 

      Van giesons stain

  • 25. 
    Which ones are true and which ones are false ?
    • A. 

      Cresyl violet is an basic aniline dye

    • B. 

      Toluidine blue is an basic aniline dye

    • C. 

      Eosin colors structures pinkish and Erythrocytes intensively red

    • D. 

      Xylene is not an dye

    • E. 

      The most common fixative is represented by(10% formalin)

    • F. 

      10% formalin is really 50% formaldehyde diluted in water

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