Histological Techniques 2

25 Questions | By Ferna | Updated: Mar 21, 2022 | Attempts: 1650

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1.

A lipid is stained using Sudan III or Sudan IV What is true ?
- A.
  
  Nuclei are counterstained with haematoxylin
- B.
  
  Nuclei are counterstained with Nuclear red
- C.
  
  Nuclei appears red
- D.
  
  Nuclei appears dark
- E.
  
  Lipids appear red
- F.
  
  Lipids appear yellow
2.

A neutral lipid is stained with Sudan III What color does it appear ?
- A.
  
  Yellow
- B.
  
  Pink
- C.
  
  Red
- D.
  
  Dark
- E.
  
  Blue
3.

Elastin is stained with which method ?
- A.
  
  Sudan III
- B.
  
  Auric chloride
- C.
  
  Perl´s reaction
- D.
  
  Aniline blue
- E.
  
  Weigert resorcin-fuchsin technique
4.

Elastin is counterstained with ? and appearing in which color(choose the 1 true)
- A.
  
  Haemotoxylin
- B.
  
  Eosin
- C.
  
  Dark
- D.
  
  Nuclear red
- E.
  
  Mayers mucicarmine
- F.
  
  Red
5.

Reticular fibers are stained using which methods ?
- A.
  
  Nuclear red
- B.
  
  Gomoroi ammoniac silver nitrate
- C.
  
  Luxol blue
- D.
  
  Haemotoxylin
- E.
  
  Eosin
6.

Blue trichrome is used to stain ?
- A.
  
  Glycogen
- B.
  
  Mucus
- C.
  
  Myelin sheats
- D.
  
  Acidic tissue structures
- E.
  
  Basic tissue structures
- F.
  
  Collagen
7.

In the following picture, the connective tissue is stained using what staining method ?
- A.
  
  Colloid iron
- B.
  
  Perls reaction
- C.
  
  Luxol blue
- D.
  
  Blue trichrome
8.

What is the first step before staining the sections ?
- A.
  
  Heat it up and place it in paraffin at 56c
- B.
  
  Rins the section out in distilled water
- C.
  
  The section must be deparaffinized in Xylene
- D.
  
  Freezing it
9.

What is true for basic stains ?
- A.
  
  They bind to cytoplasm
- B.
  
  They bind to basic structures
- C.
  
  They bind to ergastoplasm
- D.
  
  They bind to ground substance of cartilage
- E.
  
  They bind to mucus
- F.
  
  They bind to acidic tissue structures
10.

What is true about the staining method of Haemotoxylin and eosin ?
- A.
  
  Haemotoxylin stains nuclei Brown
- B.
  
  Eosin stains nuclei pink
- C.
  
  Haemotoxylin stains nuclei Dark
- D.
  
  Eosin stains structures pink
- E.
  
  Eosin stains structures red
- F.
  
  Haemotoxylin stains structures blue
- G.
  
  Eosin stains granules of eosinophilic leukocytes intensively red
11.

Which of the following are stained intensively red by Eosin ?
- A.
  
  Erythrocytes
- B.
  
  Structures
- C.
  
  Nuclei
- D.
  
  Acidophilic cells
- E.
  
  Eosin doesnt stains red
12.

Acidic stains bind to:
- A.
  
  Chromatin of the nucleus
- B.
  
  Mucus
- C.
  
  Ergastoplasm
- D.
  
  Ground substance of cartilage
13.

When sections have been stained and are prepared for covering What must be done ?
- A.
  
  Stained sections most be dehydrated with ethanol
- B.
  
  Stained sections most be rehydrated with ethanol
- C.
  
  Stained sections most be dehydrated with xylene
- D.
  
  Stained sections most De-paraffinized using xylene
- E.
  
  Stained sections most be embedded in paraffin
- F.
  
  Stained sections are mounted in Canada balsam
14.

In flourescent microscopy a sample is exposed to:
- A.
  
  Light of long wavelength
- B.
  
  Light of short wavelength
- C.
  
  Light of normal wavelength
15.

In flourescent microscopy tissue reflects light of short wavelength back as longer wavelengths. What most the tissue contain for this to happen ?
- A.
  
  Natriumflourid
- B.
  
  Fluor
- C.
  
  Fluorochrome
16.

To visualize structures that do not exhibit primary fluorescence, fluorescent dyes (fluorochromes) must be utilized; light emitted by these dyes is referred to as
- A.
  
  Quaternary fluorescence
- B.
  
  Primary fluorescence
- C.
  
  Secondary flourescence
17.

Which are nuclear dyes of the following in case of Fluorescence microscopy ?
- A.
  
  Rhodamine 123
- B.
  
  DiI
- C.
  
  Acridine orange
- D.
  
  Propidium iodide;
18.

Perls‘ reaction are used for ?
- A.
  
  Staining of glycogen
- B.
  
  Detection of Fe3+
- C.
  
  Staining of mucus
- D.
  
  Detecting phospholipides in myelin sheath
- E.
  
  Detecting Haemosiderin
- F.
  
  Staining nuclei
19.

What method is best for detection of glycogene ? and what is the color of the stain ?
- A.
  
  Red
- B.
  
  Best carmine
- C.
  
  Blue
- D.
  
  Mayers mucicarmine
- E.
  
  Purple
- F.
  
  Perls reaction
- G.
  
  Pas reaction
20.

What method is used for detection of acidic mucopolysaccharides ?
- A.
  
  Mayers mucicarmine
- B.
  
  Colloid iron according to Hale-Muller
- C.
  
  Pas method
- D.
  
  Nuclear red
- E.
  
  Haemotoxylin
21.

What is true when staining Mucus ?
- A.
  
  The stain becomes purple
- B.
  
  The counterstain is Haematoxylin
- C.
  
  The counterstain is Nuclear red
- D.
  
  The mucus stains pink
- E.
  
  The stain used is Best carmine
- F.
  
  The stain used is Mayers Mucicarmine
22.

What is true for staining pigment Melanin ?
- A.
  
  Weigert resorcin-fuchsin technique is used
- B.
  
  Green trichrome is used as an counterstain
- C.
  
  Van gieson stain is used
- D.
  
  The counterstain is Hematoxylin
- E.
  
  Argentaffin reaction is used
23.

What of the following are true ?
- A.
  
  Haematoxylin and eosin are nucleic stains
- B.
  
  Haemotoyxlin and eosin are bind to basic structures
- C.
  
  Argentaffin reaction stains structures black
- D.
  
  Myelin sheats are counterstained with nuclear red
- E.
  
  Argentaffin reaction stains Polysaccharides
- F.
  
  Green trichrome are counterstained with Haemotoxylin
24.

What one is the one true method for detection of DNA ?
- A.
  
  Hale-muller
- B.
  
  Gomori
- C.
  
  Feulgens nuclear reaction reaction
- D.
  
  Van giesons stain
25.

Which ones are true and which ones are false ?
- A.
  
  Cresyl violet is an basic aniline dye
- B.
  
  Toluidine blue is an basic aniline dye
- C.
  
  Eosin colors structures pinkish and Erythrocytes intensively red
- D.
  
  Xylene is not an dye
- E.
  
  The most common fixative is represented by(10% formalin)
- F.
  
  10% formalin is really 50% formaldehyde diluted in water