Histological Techniques 2

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Histological Techniques 2 - Quiz

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Questions and Answers
  • 1. 

    A lipid is stained using Sudan III or Sudan IV What is true ?

    • A.

      Nuclei are counterstained with haematoxylin

    • B.

      Nuclei are counterstained with Nuclear red

    • C.

      Nuclei appears red

    • D.

      Nuclei appears dark

    • E.

      Lipids appear red

    • F.

      Lipids appear yellow

    Correct Answer(s)
    A. Nuclei are counterstained with haematoxylin
    D. Nuclei appears dark
    E. Lipids appear red
    Explanation
    Sudan III or Sudan IV stains lipids, causing them to appear red. Haematoxylin is commonly used as a counterstain for nuclei, and it causes the nuclei to appear dark or blue. Therefore, the correct answer is that nuclei are counterstained with haematoxylin, nuclei appear dark, and lipids appear red.

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  • 2. 

    A neutral lipid is stained with Sudan III What color does it appear ?

    • A.

      Yellow

    • B.

      Pink

    • C.

      Red

    • D.

      Dark

    • E.

      Blue

    Correct Answer
    C. Red
    Explanation
    Sudan III is a dye commonly used to stain neutral lipids. When neutral lipids are stained with Sudan III, they appear red in color.

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  • 3. 

    Elastin is stained with which method ?

    • A.

      Sudan III

    • B.

      Auric chloride

    • C.

      Perl´s reaction

    • D.

      Aniline blue

    • E.

      Weigert resorcin-fuchsin technique

    Correct Answer
    E. Weigert resorcin-fuchsin technique
    Explanation
    The Weigert resorcin-fuchsin technique is used to stain elastin. This technique involves the use of resorcin-fuchsin dye, which specifically binds to elastin fibers and stains them a deep purple color. This staining method is commonly used in histology and pathology to visualize and study elastin in tissues.

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  • 4. 

    Elastin is counterstained with ? and appearing in which color(choose the 1 true)

    • A.

      Haemotoxylin

    • B.

      Eosin

    • C.

      Dark

    • D.

      Nuclear red

    • E.

      Mayers mucicarmine

    • F.

      Red

    Correct Answer(s)
    D. Nuclear red
    F. Red
    Explanation
    Elastin is counterstained with Nuclear red, which appears as red in color. This staining technique is commonly used in histology to visualize elastin fibers, as elastin itself does not readily stain with common histological stains. Nuclear red is specifically chosen as a counterstain because it stains the nuclei of cells, allowing for better contrast and visibility of the elastin fibers. The combination of Nuclear red staining and the red color it produces helps to highlight and distinguish elastin fibers in tissue samples.

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  • 5. 

    Reticular fibers are stained using which methods ?

    • A.

      Nuclear red

    • B.

      Gomoroi ammoniac silver nitrate

    • C.

      Luxol blue

    • D.

      Haemotoxylin

    • E.

      Eosin

    Correct Answer(s)
    A. Nuclear red
    B. Gomoroi ammoniac silver nitrate
    Explanation
    The correct answer for staining reticular fibers is nuclear red and Gomoroi ammoniac silver nitrate. Reticular fibers are a type of connective tissue fibers that provide support to various organs. Nuclear red is a dye that selectively stains the nuclei of cells, allowing for visualization of the reticular fibers. Gomoroi ammoniac silver nitrate is a silver staining method that specifically targets reticular fibers, making them easily distinguishable under a microscope.

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  • 6. 

    Blue trichrome is used to stain ?

    • A.

      Glycogen

    • B.

      Mucus

    • C.

      Myelin sheats

    • D.

      Acidic tissue structures

    • E.

      Basic tissue structures

    • F.

      Collagen

    Correct Answer
    F. Collagen
    Explanation
    Blue trichrome stain is commonly used to visualize collagen fibers in tissue samples. Collagen is a major component of connective tissues, such as tendons, ligaments, and cartilage. This stain helps to highlight and differentiate collagen fibers from other tissue structures, allowing researchers and pathologists to study the distribution and organization of collagen in various tissues.

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  • 7. 

    In the following picture, the connective tissue is stained using what staining method ?

    • A.

      Colloid iron

    • B.

      Perls reaction

    • C.

      Luxol blue

    • D.

      Blue trichrome

    Correct Answer
    D. Blue trichrome
    Explanation
    The correct answer is Blue trichrome because it is a staining method used to stain connective tissue.

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  • 8. 

    What is the first step before staining the sections ?

    • A.

      Heat it up and place it in paraffin at 56c

    • B.

      Rins the section out in distilled water

    • C.

      The section must be deparaffinized in Xylene

    • D.

      Freezing it

    Correct Answer
    C. The section must be deparaffinized in Xylene
    Explanation
    Before staining the sections, the first step is to deparaffinize the section in Xylene. Deparaffinization is the process of removing the paraffin wax used in tissue embedding during the preparation of histological sections. Xylene is a common solvent used for this purpose as it effectively dissolves the paraffin wax. By deparaffinizing the sections, the wax is removed, allowing the stain to penetrate the tissue and provide accurate and clear results during staining.

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  • 9. 

    What is true for basic stains ?

    • A.

      They bind to cytoplasm

    • B.

      They bind to basic structures

    • C.

      They bind to ergastoplasm

    • D.

      They bind to ground substance of cartilage

    • E.

      They bind to mucus

    • F.

      They bind to acidic tissue structures

    Correct Answer(s)
    C. They bind to ergastoplasm
    D. They bind to ground substance of cartilage
    F. They bind to acidic tissue structures
    Explanation
    Basic stains are positively charged and therefore bind to negatively charged structures in cells and tissues. They are attracted to acidic tissue structures such as nucleic acids and certain proteins. They also bind to ergastoplasm, which is a specialized region of the endoplasmic reticulum involved in protein synthesis. Additionally, basic stains can bind to the ground substance of cartilage, which is a gel-like substance that fills the spaces between cells.

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  • 10. 

    What is true about the staining method of Haemotoxylin and eosin ?

    • A.

      Haemotoxylin stains nuclei Brown

    • B.

      Eosin stains nuclei pink

    • C.

      Haemotoxylin stains nuclei Dark

    • D.

      Eosin stains structures pink

    • E.

      Eosin stains structures red

    • F.

      Haemotoxylin stains structures blue

    • G.

      Eosin stains granules of eosinophilic leukocytes intensively red

    Correct Answer(s)
    C. Haemotoxylin stains nuclei Dark
    D. Eosin stains structures pink
    G. Eosin stains granules of eosinophilic leukocytes intensively red
    Explanation
    Haemotoxylin is a stain that is commonly used in histology to stain nuclei. It binds to the DNA in the nuclei and stains them a dark color, typically blue. Eosin, on the other hand, is used to stain the cytoplasm and other structures in the tissue. It stains them pink or red, depending on the intensity. In addition, eosin can also stain the granules of eosinophilic leukocytes, which are a type of white blood cell, intensively red. Therefore, the correct answer is that Haemotoxylin stains nuclei dark, Eosin stains structures pink, and Eosin stains granules of eosinophilic leukocytes intensively red.

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  • 11. 

    Which of the following are stained intensively red by Eosin ?

    • A.

      Erythrocytes

    • B.

      Structures

    • C.

      Nuclei

    • D.

      Acidophilic cells

    • E.

      Eosin doesnt stains red

    Correct Answer(s)
    A. Erythrocytes
    D. Acidophilic cells
    Explanation
    Eosin is a red dye commonly used in histology to stain acidic structures, such as eosinophils and erythrocytes. Erythrocytes, also known as red blood cells, are stained intensively red by eosin due to their high acidophilia. Acidophilic cells, which are cells that have an affinity for acidic dyes like eosin, are also stained intensively red. Therefore, both erythrocytes and acidophilic cells are stained red by eosin.

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  • 12. 

    Acidic stains bind to:

    • A.

      Chromatin of the nucleus

    • B.

      Mucus

    • C.

      Ergastoplasm

    • D.

      Ground substance of cartilage

    Correct Answer
    B. Mucus
    Explanation
    Acidic stains are commonly used in histology and microscopy to stain acidic components in tissues. Mucus is a slimy substance produced by goblet cells and other epithelial cells, which is known to have acidic properties. Therefore, acidic stains are likely to bind to mucus, making it the correct answer in this context.

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  • 13. 

    When sections have been stained and are prepared for covering What must be done ?

    • A.

      Stained sections most be dehydrated with ethanol

    • B.

      Stained sections most be rehydrated with ethanol

    • C.

      Stained sections most be dehydrated with xylene

    • D.

      Stained sections most De-paraffinized using xylene

    • E.

      Stained sections most be embedded in paraffin

    • F.

      Stained sections are mounted in Canada balsam

    Correct Answer(s)
    A. Stained sections most be dehydrated with ethanol
    C. Stained sections most be dehydrated with xylene
    F. Stained sections are mounted in Canada balsam
    Explanation
    After the sections have been stained and prepared for covering, they need to be dehydrated with ethanol to remove any remaining water before further processing. This is necessary to prevent any water-based solutions from diluting the mounting medium or causing artifacts. Additionally, the sections must also be dehydrated with xylene to remove the ethanol and prepare them for embedding in paraffin. Finally, the stained sections are mounted in Canada balsam, which is a common mounting medium used in histology to provide a clear and permanent cover for the sections.

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  • 14. 

    In flourescent microscopy a sample is exposed to:

    • A.

      Light of long wavelength

    • B.

      Light of short wavelength

    • C.

      Light of normal wavelength

    Correct Answer
    B. Light of short wavelength
    Explanation
    Fluorescent microscopy involves the use of fluorophores, which are molecules that emit light of a longer wavelength when excited by light of a shorter wavelength. Therefore, in fluorescent microscopy, the sample is exposed to light of a short wavelength, which causes the fluorophores in the sample to emit light of a longer wavelength. This emitted light is then detected and used to create an image of the sample.

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  • 15. 

    In flourescent microscopy tissue reflects light of short wavelength back as longer wavelengths. What most the tissue contain for this to happen ?

    • A.

      Natriumflourid

    • B.

      Fluor

    • C.

      Fluorochrome

    Correct Answer
    C. Fluorochrome
    Explanation
    The correct answer is fluorochrome. Fluorochromes are fluorescent dyes or molecules that emit light of a longer wavelength when excited by light of a shorter wavelength. In fluorescent microscopy, fluorochromes are often used to label specific molecules or structures within the tissue. When the tissue containing fluorochromes is excited by a short wavelength light source, the fluorochromes absorb the light and re-emit it as longer wavelength light, allowing for visualization and analysis of the labeled structures.

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  • 16. 

    To visualize structures that do not exhibit primary fluorescence, fluorescent dyes (fluorochromes) must be utilized; light emitted by these dyes is referred to as

    • A.

      Quaternary fluorescence

    • B.

      Primary fluorescence

    • C.

      Secondary flourescence

    Correct Answer
    C. Secondary flourescence
    Explanation
    Secondary fluorescence refers to the light emitted by fluorescent dyes (fluorochromes) that are used to visualize structures that do not exhibit primary fluorescence. Primary fluorescence refers to the natural fluorescence exhibited by certain structures, while quaternary fluorescence is not mentioned in the given information. Therefore, the correct answer is secondary fluorescence.

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  • 17. 

    Which are nuclear dyes of the following in case of Fluorescence microscopy ?

    • A.

      Rhodamine 123

    • B.

      DiI

    • C.

      Acridine orange

    • D.

      Propidium iodide;

    Correct Answer(s)
    C. Acridine orange
    D. Propidium iodide;
    Explanation
    Acridine orange and propidium iodide are nuclear dyes commonly used in fluorescence microscopy. These dyes have the ability to bind to nucleic acids, specifically DNA, and emit fluorescence when excited by specific wavelengths of light. Acridine orange is a metachromatic dye that emits green fluorescence when bound to DNA, while propidium iodide emits red fluorescence. These dyes are widely used for staining and visualizing the nucleus in cells, allowing researchers to study nuclear structure and function.

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  • 18. 

    Perls‘ reaction are used for ?

    • A.

      Staining of glycogen

    • B.

      Detection of Fe3+

    • C.

      Staining of mucus

    • D.

      Detecting phospholipides in myelin sheath

    • E.

      Detecting Haemosiderin

    • F.

      Staining nuclei

    Correct Answer(s)
    B. Detection of Fe3+
    E. Detecting Haemosiderin
    Explanation
    Perls' reaction is used for the detection of Fe3+ and Haemosiderin. Fe3+ detection is important in various biological and chemical processes, while Haemosiderin is an iron-storage protein found in cells. The Perls' reaction helps in identifying the presence of these substances through a staining method. It is not used for staining glycogen, mucus, phospholipids in myelin sheath, or nuclei.

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  • 19. 

    What method is best for detection of glycogene ? and what is the color of the stain ?

    • A.

      Red

    • B.

      Best carmine

    • C.

      Blue

    • D.

      Mayers mucicarmine

    • E.

      Purple

    • F.

      Perls reaction

    • G.

      Pas reaction

    Correct Answer(s)
    B. Best carmine
    E. Purple
    G. Pas reaction
    Explanation
    The best method for detection of glycogene is the Pas reaction, which involves staining the glycogene with a specific dye called Best carmine. This staining procedure results in a purple color, allowing for easy identification and detection of glycogene.

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  • 20. 

    What method is used for detection of  acidic mucopolysaccharides ?

    • A.

      Mayers mucicarmine

    • B.

      Colloid iron according to Hale-Muller

    • C.

      Pas method

    • D.

      Nuclear red

    • E.

      Haemotoxylin

    Correct Answer(s)
    B. Colloid iron according to Hale-Muller
    D. Nuclear red
  • 21. 

    What is true when staining Mucus ?

    • A.

      The stain becomes purple

    • B.

      The counterstain is Haematoxylin

    • C.

      The counterstain is Nuclear red

    • D.

      The mucus stains pink

    • E.

      The stain used is Best carmine

    • F.

      The stain used is Mayers Mucicarmine

    Correct Answer(s)
    B. The counterstain is Haematoxylin
    D. The mucus stains pink
    F. The stain used is Mayers Mucicarmine
    Explanation
    When staining mucus, the counterstain used is Haematoxylin, which is a blue dye that helps to enhance the contrast of the stained mucus. The mucus itself stains pink, indicating its presence and location. The specific stain used for mucus staining is Mayers Mucicarmine, which is a combination of carmine dye and mucicarmine. This stain is commonly used to differentiate mucus-producing cells and to identify certain types of tumors.

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  • 22. 

    What is true for staining pigment Melanin ?

    • A.

      Weigert resorcin-fuchsin technique is used

    • B.

      Green trichrome is used as an counterstain

    • C.

      Van gieson stain is used

    • D.

      The counterstain is Hematoxylin

    • E.

      Argentaffin reaction is used

    Correct Answer
    E. Argentaffin reaction is used
    Explanation
    The correct answer is that the Argentaffin reaction is used for staining pigment Melanin. This technique is commonly used to detect and visualize melanin in tissues. The reaction involves the use of silver salts, which react with the melanin pigment to produce a brown or black color. This staining method is specifically designed to target and highlight melanin, making it easier to identify and study in histological samples.

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  • 23. 

    What of the following are true ?

    • A.

      Haematoxylin and eosin are nucleic stains

    • B.

      Haemotoyxlin and eosin are bind to basic structures

    • C.

      Argentaffin reaction stains structures black

    • D.

      Myelin sheats are counterstained with nuclear red

    • E.

      Argentaffin reaction stains Polysaccharides

    • F.

      Green trichrome are counterstained with Haemotoxylin

    Correct Answer(s)
    A. Haematoxylin and eosin are nucleic stains
    C. Argentaffin reaction stains structures black
    F. Green trichrome are counterstained with Haemotoxylin
    Explanation
    Haematoxylin and eosin are nucleic stains because they are commonly used to stain the nuclei of cells in histology. Argentaffin reaction stains structures black, as it is a method used to selectively stain structures containing certain types of chemicals, such as catecholamines, which appear black under the reaction. Green trichrome is counterstained with Haematoxylin, meaning that after the green trichrome stain is applied, the nuclei of the cells are stained with Haematoxylin to provide contrast.

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  • 24. 

    What one is the one true method for detection of DNA ?

    • A.

      Hale-muller

    • B.

      Gomori

    • C.

      Feulgens nuclear reaction reaction

    • D.

      Van giesons stain

    Correct Answer
    C. Feulgens nuclear reaction reaction
    Explanation
    Feulgen's nuclear reaction is the one true method for the detection of DNA. This reaction specifically stains DNA, allowing for its visualization under a microscope. It is based on the principle that DNA reacts with a specific dye, known as Feulgen's reagent, to form a colored complex. This staining technique is widely used in various fields, including genetics and pathology, to identify and study DNA within cells and tissues.

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  • 25. 

    Which ones are true and which ones are false ?

    • A.

      Cresyl violet is an basic aniline dye

    • B.

      Toluidine blue is an basic aniline dye

    • C.

      Eosin colors structures pinkish and Erythrocytes intensively red

    • D.

      Xylene is not an dye

    • E.

      The most common fixative is represented by(10% formalin)

    • F.

      10% formalin is really 50% formaldehyde diluted in water

    Correct Answer(s)
    A. Cresyl violet is an basic aniline dye
    B. Toluidine blue is an basic aniline dye
    C. Eosin colors structures pinkish and Erythrocytes intensively red
    D. Xylene is not an dye
    E. The most common fixative is represented by(10% formalin)
    Explanation
    Cresyl violet and Toluidine blue are both basic aniline dyes, which means they have a positive charge and are commonly used in histology to stain acidic components of cells. Eosin is a dye that colors structures pinkish and intensively stains red blood cells. Xylene, on the other hand, is not a dye but a solvent commonly used in histology to dehydrate and clear tissue samples. The most common fixative used is 10% formalin, which is a solution of formaldehyde diluted in water.

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