Block 9 Micro Viral Replication Prt 1

20 Questions | Total Attempts: 217

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Block 9 Micro Viral Replication Prt 1

Questions and Answers
  • 1. 
    Which viral element from previous question would determine cell susceptibility?
    • A. 

      Capsid

    • B. 

      (+) ssRNA

    • C. 

      RNA-dependent RNA polymerase

    • D. 

      Matrix

    • E. 

      G protein

  • 2. 
    Which enzyme is required for the replication of dsDNA viruses?
    • A. 

      DNA-dependent RNA polymerase

    • B. 

      RNA-dependent DNA polymerase

    • C. 

      RNA-dependent RNA polymerase

    • D. 

      DNA-dependent DNA polymerase

    • E. 

      Reverse transcriptase

  • 3. 
    What is the virion structure indicated by the arrow?
    • A. 

      Envelope

    • B. 

      Nucleus

    • C. 

      Tegument

    • D. 

      Capsid

    • E. 

      Matrix

  • 4. 
    What is the function of the structure identified in question 2?
    • A. 

      Transcription of genomic cargo

    • B. 

      Binding of virion to cell

    • C. 

      Membrane fusion and entry

    • D. 

      Translation of genomic cargo

    • E. 

      Protection of genomic cargo

  • 5. 
    What would have been the function(s) of the structure identified in question 2 if the virus had been naked?
    • A. 

      Protection of genomic cargo

    • B. 

      Binding of virion to cell

    • C. 

      Membrane fusion and entry

    • D. 

      Protection of genomic cargo and cell binding

  • 6. 
    What enzyme is required for the replication of (-) ssRNA viruses?
    • A. 

      DNA-dependent RNA polymerase

    • B. 

      RNA-dependent DNA polymerase

    • C. 

      RNA-dependent RNA polymerase

    • D. 

      DNA-dependent DNA polymerase

    • E. 

      Reverse transcriptase

  • 7. 
    Hepatitis B virus (HBV; Hepadnaviridae), the only known gapped DNA virus to infect humans, requires which polymerases to complete its replicative cycle?
    • A. 

      Cellular RNA-dependent RNA polymerase and reverse transcriptase

    • B. 

      Cellular DNA-dependent RNA polymerase and reverse transcriptase

    • C. 

      Viral RNA-dependent RNA polymerase and reverse transcriptase

    • D. 

      Viral DNA-dependent RNA polymerase and reverse transcriptase

    • E. 

      Viral RNA-dependent DNA polymerase and reverse transcriptase

  • 8. 
    Retroviruses such as the Human immunodeficiency virus type 1 (HIV-1) require which polymerases to complete their replication cycle?
    • A. 

      Viral RNA-dependent DNA polymerase and cellular RNA-dependent RNA polymerase

    • B. 

      Viral RNA-dependent DNA polymerase and viral RNA-dependent RNA polymerase

    • C. 

      Viral RNA-dependent DNA polymerase and cellular DNA-dependent RNA polymerase

    • D. 

      Cellular RNA-dependent DNA polymerase and viral DNA-dependent RNA polymerase

    • E. 

      Cellular RNA-dependent DNA polymerase and viral RNA-dependent DNA polymerase

  • 9. 
    A virology research lab (Dead End Empirical Technologies Inc.) engineers a composite virus with the following characteristics: The virion possesses a capsid derived from Human papillomavirus type 16 (HPV-16; Papillomaviridae) containing Rhinovirus type 99 (Picornaviridae) (+) ssRNA and Hepatitis A virus (HAV; Picornaviridae) RNA-dependent RNA polymerase. The nucleocapsid is confined into a Human immunodeficiency virus type 1 (HIV-1; Retroviridae) matrix, which is in turn surrounded by an envelope derived from HEK 293 cells (human embryonic kidney 293 cells) pseudotyped with murine vesicular stomatitis virus (VSV; Rhabdoviridae) G proteins (i. e. the viral envelope contains VSV G proteins). If this virion were to infect permissive cells, which kind of virus would be generated?
    • A. 

      HPV-16

    • B. 

      Rhinovirus type 99

    • C. 

      HAV

    • D. 

      HIV-1

    • E. 

      VSV

  • 10. 
    Which viral element from question 8 would determine cell susceptibility?
    • A. 

      Capsid

    • B. 

      (+) ssRNA

    • C. 

      RNA-dependent RNA polymerase

    • D. 

      Matrix

    • E. 

      G protein

  • 11. 
    What would be the composition of the progeny virus?
    • A. 

      (+) ssRNA & capsid

    • B. 

      (+) ssRNA, capsid & envelope

    • C. 

      (+) ssRNA, capsid, matrix & envelope

    • D. 

      (+) ssRNA, polymerase, capsid, matrix & envelope

    • E. 

      (+) ssRNA, polymerase & capsid

    • F. 

      (+) ssRNA, polymerase, capsid & envelope

  • 12. 
    7.1) In theory, what would be the outcome of a non-vaccinated, laboratory technician accidentally infecting him(her)self with a liquid suspension of Measles virus (+) ssRNA contained in a syringe?
    • A. 

      Viral replication/Disease

    • B. 

      Viral replication/No disease

    • C. 

      No viral replication/Disease

    • D. 

      No viral replication/No disease

    • E. 

      A & B

  • 13. 
    7.2) In theory, what would be the outcome of a non-vaccinated, laboratory technician accidentally infecting him(her)self with a liquid suspension of Ebola virus (-) ssRNA contained in a syringe?
    • A. 

      Viral replication/Disease

    • B. 

      Viral replication/No disease

    • C. 

      No viral replication/Disease

    • D. 

      No viral replication/No disease

  • 14. 
    7.3) Under natural conditions (i. e. not in laboratory or immunization settings), which of the following viruses would be the simplest virus capable of completing its infectious cycle?
    • A. 

      A virus that would consist of its nucleic acid and its capsid

    • B. 

      A virus that would consist of its nucleic acid, its corresponding polymerase and its capsid

    • C. 

      A virus that would consist of its nucleic acid, its corresponding polymerase, its capsid and its envelope

    • D. 

      A virus that would consist of its nucleic acid, its corresponding polymerase, its capsid, its matrix and its envelope

    • E. 

      A virus that would consist of its nucleic acid, its corresponding polymerase, its capsid, its matrix, its tegument and its envelope

  • 15. 
    Which statement best applies to virions?
    • A. 

      Virions are the vessels that protect and deliver viral self-replicating proteins from susceptible host cells to other susceptible host cells.

    • B. 

      Virions are the vessels that protect and deliver viral self-replicating proteins from permissive host cells to other permissive host cells

    • C. 

      Virions are vessels that protect and deliver viral genomes from susceptible host cells to other susceptible host cells.

    • D. 

      Virions are vessels that protect and deliver viral genomes from permissive host cells to other permissive host cells.

    • E. 

      Virions are vessels that need to be processed by viral proteases in order to become fully infectious viruses.

  • 16. 
    Which of the following accurately describe(s) the actual order(s) of (a) viral replication cycle(s)? (More than one answer is possible)
    • A. 

      Attachment---Entry---Uncoating---Genome replication---Assembly---Release---Maturation

    • B. 

      Attachment---Entry---Genome replication---Uncoating---Assembly---Release---Maturation

    • C. 

      Attachment---Entry---Uncoating---Genome replication---Release---Assembly---Maturation

    • D. 

      Attachment---Uncoating---Entry---Genome replication---Assembly---Release---Maturation

    • E. 

      Attachment---Entry---Uncoating---Assembly---Genome replication---Release

  • 17. 
    Which of the following accurately describes cell susceptibility to viral infection?
    • A. 

      Host cell possesses all the components required for viral replication

    • B. 

      Host cell possesses the proper receptor(s) required for viral binding

    • C. 

      Host cell possesses the appropriate polymerase(s) required for viral replication

    • D. 

      Host cell possesses the elements required for initiating viral uncoating

    • E. 

      Host cell possesses the appropriate cellular transport machinery required for trafficking viral subunits to sites of virion assembly

  • 18. 
    IN GENERAL, what is the order of the events occurring during sequential viral assembly?
    • A. 

      Capsid protein synthesis first---Genome replication second---Packaging of genome into preformed capsid third

    • B. 

      Genome replication first---Capsid protein synthesis second---Packaging of genome into preformed capsid third

    • C. 

      Capsid protein synthesis first---Packaging of genome into preformed capsid second---Genome replication third

    • D. 

      Capsid proteins are synthesized while the genome is being replicated, and assembly progresses as the capsid subunits are formed and the genome elongated.

    • E. 

      Viral assembly is a series of random events taking place invariably in the cytosol, i. e. there is no order.

  • 19. 
    Which of the following assertions is false?
    • A. 

      Viral packaging requires packaging signals

    • B. 

      Viral packaging relates to the incorporation of the genomic nucleic acid into the capsid.

    • C. 

      Viral packaging can be done prior to capsid formation

    • D. 

      Viral assembly relates to the incorporation of virally encoded enzymes into the capsid.

    • E. 

      Viral assembly relates to the incorporation of tRNAs.

  • 20. 
    Maraviroc is an FDA (Food and Drug Administration) approved chemokine receptor antagonist that binds to human chemokine receptor CCR5. Given the function of CCR5 in the replication cycle of Human immunodeficiency virus type 1 (HIV-1), which event is affected by this drug?
    • A. 

      Entry

    • B. 

      Uncoating

    • C. 

      Assembly

    • D. 

      Release

    • E. 

      Maturation