Bases, pentoses, and phosphates all contribute
Only bases
Only bases and pentoses
Only pentoses and phosphates
TTAAGGCC
CCGGAATT
UUAAGGCC
CCGGAAUU
MRNA
TRNA
SS RNA
All rRNA's
MRNA
TRNA
RRNA
SnRNA
AGP, pAC, pAA
AGPp, AC, pAA
AGp, TAp, CAA
AG, TAp, pCCA
DNA polymerase adds nucleotides in a 5' --> 3' direction
A primer strand of DNA must contain a free 3'-OH end
The primer strand of DNA determines which nucleotides are added next
The correct complementary hydrogen bonding between base pairs is the primary check on the fidelity of the newly synthesized DNA
Decreasing the concentration of DNA
Increasing the concentration of NaCl in the solution
Adding 1% urea (an organic denaturant)
All of the above
The sugars are located in the interior of the double helix
B
A left-handed helix
it has 12 bases per turn
It is found in vivox
All of the above
500
50
450
350
Changing the temperature
Increasing salt concentration
The action of topoisomerase
The action of a nuclease such as pancreatic DNAse
Higher salt concentration in the buffer
Higher GC content in the DNA
PH higher than 10 in the buffer
Longer DNA fragments
ATGCATGCATGCATGC / TACGTACGTACGTACG
AAAAAAAAACCCCCCCCC / TTTTTTTTTGGGGGGGGC
ATATATATGCGCGCGCGC / TATATATACGCGCGCGCG
CATTAAGCAGTGCTTAAGA / GAATTCGTCACGAATTCT
The absorbance at 260 decreases about 40%
The hgiher the G+C content, the higher the melting temperature
The transition from dsDNA to ssDNA is highly cooperative
The fraction of DNA that is single stranded is 0.5 at its Tm
It serves as the start point for the new DNA strand
It serves as a guide in determining the next nucleotide to be added by the polymerase
It increases the processivity of the DNA polymerase
It is essential for the 3-5' exonuclease activity
It recognizes the abnormality in the duplex DNA structure.
It nicks the DNA to provide a 3'-OH
It removes the damaged section of DNA with its 5'-3' exonuclease activity
It ligates the DNA after the repair synthesis
It insures the fidelity of the newly synthesized DNA strand
The DNA polymerases require a preexisting strand with a nucleotide having a 3'-OH.
The DNA polymerases require a preexisting strand with a nucleotide having a 5'-OH.
All of the above are correct
Are recessed with 5'-phosphates
Are recessed with 3'-OHs
Are extended with 5'-phosphates
Are extended with 3'-OHs
DNA polymerase I
Klenow fragment
DNA polymerase II
DNA polymerase III
DNA polymerase activity
5'-3' exonuclease activity
The large fragment from the C-terminal end of the polymerase
3'-5' exonuclease activity
Is primarily carried out by DNA polymerase I
Is synthesized continuously
This DNA strand is synthesized in a 3'-5' direction of synthesis
Is initially synthesized as Okazaki fragments
It syntheslzes the RNA primer in DNA repllcation.
It is a specific RNA polymerase
It is essential for DNA replication
It is also a gyrase
DNA polymerase alpha
DNA polymerase alpha
DNA polymerase delta
DNA polymerase alpha
It is responsible for incorporating most of the nucleotides in the lagging strand
It synthesizes most of the leading strand prior to aiding in the synthesis of the lagging strand
It contains a 3' to 5' exonuclease activity
It synthesizes the leading strand and the lagging strand at the same time
Primase
DNA polymerase I
DNA polymerase III
DNA gyrase
The cell contains more molecules of DNA pol I than DNA pol III
DNA pol I is a single polypeptide with three domains
DNA pol I is involved in the repair of UV damaged DNA
DNA pol I has a larger turnover number than DNA pol III
It functions int he replication but not in the repair of DNA
It forms a "DNA-AMP" covalent complex during the reaction it catalyzes
It can seal nicks in the dsDNA as long as the adjacent nucleotides surrounding the nick contain a free 3' hydroxyl and a 5' phosphate
It catalyzes the formation of a phosphoester bond in dsDNA
RNA polymerase
DNA polymerase I
DNA polymerase III
Klenow fragment from DNA polymerase I
GCGCGCGCGCGC / CGCGCGCGCGCG
GGGGGGGGCGA / CCCCCCCCGCC
ATGCATGC / TACGTACG
GGCCGGCCGG / CCGGCCGGCC
The dNTP hydrolysis to produce the incorporated NMP and the PPi
The nature of the strong connecting bonds formed int he linked nucleotides
The dNTP hydrolysis and the subsequent hydrolysis of PPi by pyrophosphatase
The inherent stability of the synthesized DNA polynucleotide
The start base for transcription in DNA
The binding site in DNA for regulatory proteins which stimulate or further promote the level of transcription
The site on DNA at which RNA polymerase binds to initiate transcription
The general region of DNA downstream from the start site.
Alpha, beta, and beta'
Alpha, beta, beta', and gamma holoenzyme
TFIID, SP1 and enhancer protein
TF IIIA, TFIIIB, and TFIIIC
Alpha
Beta
Beta prime
Gamma
Is located int he nucleolus and transcribes the major ribosomal RNA genes
Is located in the nucleoplasm (general nucleus) and transcribes protein-encoding genes
Transcribes the 5S RNA genes
Transcribes RNAs associated with mRNA processing (SNRP's)
The structural genes for lacZ, lacY, and lacA
The three structural genes listed in (a) and the operator and promoter sequences for these genes
All elements listed in (b) and the lacI gene
All elements in (c) and the promoter for lacI
They can occur within a gene
They can occur either 3' or 5' to the gene
They can be in either orientation
They can be placed at different distances from the gene without changing their influence
A
B
The rho protein
DNA polymerase
Ribosomes
RNA polymerase holoenzyme
Lac 1
Lac 2
Gene coding for the gamma-subunit of RNA polymerase
Gene encoding the catabolite activator protein
It transcribes the DNA sense strand
It transcribes the DNA antisense strand
It transcribes only protein genes
It translates DNA into protein
It consists of approximately 60 bp of dsDNA
It is recognized and bound by the gamma-subunit of RNA polymerase holoenzyme
The base sequences centered at -10 of various promoters are not identical but almost always differ by two or fewer bases from the consensus sequence
It contains a "GC rich" consensus seuqence centered at =10 called the Pribnow box
Uracyl forms unstable hydrogen bonds
Cytosine spontaneously becomes uracyl
Thymine can do the same function as uracyl
Uracyl can only from glycosidic bonds with ribose
AATCGATTCGGGCTATA
TTAGCTAAGCCCGATAT
The primer strand plus TTAGCTAAGCCCG
TTAGCTAAGCCCG
Gyrase
Topoisomerase II
Helicase
Topoisomerase I
DNA Polymerase III
DNA Polymerase gamma
DNA Polymerase delta
Gene families
An operon
Housekeeping genes
Oncogenes
Any place int he DNA after the promoter site
Any place in the DNA at the noncoding region
A consensus sequence that goes from 3' to 5'
Any region in the DNA that is closer to the 5' end
Decreases the size of the telomeres
Extends the 3' end of the telomeres lagging strand
It is not found in vertebrates
Uses a DNA primer to enxtend DNA
Lac I
Lac 2
Gene coding for the gamma-subunit of RNA polymerase
Gene encoding the catabolite activator protein
It does not contain a 3' or 5' exonuclease activity
Its holoenzyme contains four different subunits organized as alpha beta beta prime, gamma
It synthesizes mRNA, tRNA, rRNA as well as the RNA primers during replication
It uses the nucleotides UTP, GTP, ATP, and CTP as substrates
Quiz Review Timeline +
Our quizzes are rigorously reviewed, monitored and continuously updated by our expert board to maintain accuracy, relevance, and timeliness.
Wait!
Here's an interesting quiz for you.