Biochemistry 3 (Tests)

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1. How many helical turns (the linking number) are found in a relaxed circular DNA of 4500 bp?
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2. The linking number of a highly SUPERCOILED plasmid can be readily changed WITHOUT producing a relaxed DNA by:
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3. In duplex DNA, how many hydrogen bonds does thymine use?
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4. The nucleic acid that is unstable in ACID is
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5. A feature which is distinctive to Z-DNA is
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6. Which of the following characteristic IS NOT associated with E. coli PRIMASE
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7. Which statement is INCORRECT about eh synthesis of RNA by RNA polymerase in E. coli?
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8. A DNA is found to exhibit a melting temperature of 75 C in 0.1 M NaCl.  Under what experimental conditions would the Tm be INCREASED?
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9. Which one of the following characteristics IS NOT associated with E. coli PRIMASE?
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10. If you found a DNA molecule containing URACYL, the most probably CAUSE for it would be:
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11. When DNA is placed in dilute acid, it gets
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12. The molecule that carries the pure genetic message in eukaryotes is:
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13. A feature which is distinctive to Z-DNA is
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14. The polymerase which plays a similar role as that of Prokaryotic Pol III in eukaryotic replication is (pg 1006)
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15. Which item of experimental evidence gives the most support to the conclusion that "Every PURINE BASE pairs with a PYRIMIDINE BASE"
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16. The component (s) of DNA which is responsible for the absorbanc band at 250-270 nm is
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17. All are true of the DNA double helix EXCEPT:
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18. The RNA molecules which are involved in PROCESSING the hnRNA are
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19. A strain of E.coli produces the same high level of beta-galactosidase regardless of concentration of lactose in the medium. This strain most likely contains a deltion mutation int he gene (Note: you may assume that deletion mutation causes normal function
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20. In the repair of DNA, the E.coli DNA polymerase involved performs which function in addition to its polymerase activity?
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21. Which statement is INCORRECT about the STRUCTURE of native E. COLI chromosomal DNA?
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22. Why is a primer strand needed in DNA replication?
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23. E. coli replication on the lagging strand (pg 1002)
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24. DNA pol III  REPLICATES the LAGGING strand by using
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25. E. coli REPLICATION on the LAGGING strand
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26. DOUBLE-stranded RNA is found in?
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27. The linking number of a highly supercoiled plasmid can be readily changed without producing a relaxed DNA by
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28. Which one of the following characteristics ls not associated with E.coli primase?
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29. Which IS NOT a property of E. coli DNA Polymerase III?
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30. The Pribnow box is located
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31. In eukaryotes, there are many MORE PROTEINS than GENES.  This can be explained by
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32. The KLENOW fragment of E. Coli DNA Polymerase I can be used to ADD nucleotides to a DNA WITHOUT staggered ends,  IF THE ENDS...
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33. In eukaryotic cells, the only RNA molecule which has a poly (A) tail attached to the 3' end is
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34. A DNA is found to exhibit a melting temperature of 75 degrees Celsius in 0.1M NaCl.  Under what experimental conditions would the Tm be increased? (pg 372)
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35. INCREASE in gene expression in response to a METABOLITE is called:
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36. A HELICASE is characterized by:
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37. Which statement is INCORRECT about E. coli DNA Polymerase I?
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38. IN THE REPAIR of DNA, the E.coli DNA POLYMERASE involved performs which function IN ADDITION to its polymerase activity?
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39. One of the following is used for protein synthesis:
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40. In order to ACTIVATE the catabolite activator protein (CAP), it has to be
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41. Telomerase is found in humans in
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42. The reason why DNA is stable in BASE and RNA is not is because
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43. What is the primary role of the template strand in DNA replication?
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44. Why is a primer strand needed in DNA replication?
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45. Which E. coli ENZYME has the GREATEST FREQUENCY of incorporating the INCORRECT NUCLEOTIDE into is nucleic acid POLYMER?
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46. The TUS protein is a type of
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47. A GENE is a DNA SEQUENCE that encodes for:
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48. E. coli DNA polymerase I has all of the following characteristics EXCEPT:
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49. POSITIVE control of the LAC operon is ACHIEVED by:
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50. Which E. coli enzyme has the greatest frequency of incorporating the incorrect nucleotide into its nucleic acid polymer?
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51. Pure DNA has an absorbance ratio of:
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52. Which of the following characteristics is associated with the B form of DNA?
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53. Which of the following IS NOT a characteristic for DNA synthesis?
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54. To what target sequence on DNA is the oligonucleotide pAATTCCGG complementary? (sequence are written as 5'-3')
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55. The RNA molecules which are involved in processing the hnRNA are
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56. Which of the following is not a characteristic for DNA synthesis?
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57. Which of the following enzymes possesses a 5' to 3' exonuclease activity?
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58. In eukaryotes, a DNA is found in:
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59. The CLOSE promoter COMPLEX is
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60. Partial digestion of the oligonucleotide d-AGTACAA by pancreatic phosphodiesterase (a "b" cutter) would result in which set of products?
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61. In the Sanger's DNA sequencing method, what is the reason for the stop of the chain elongation?
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62. In the repair of DNA, the E.coli DNA polymerase invovled performs which function in addition to its polymerase activity?
Submit
63. Which statement is INCORRECT about the structure of native E.coli chromosomal DNA?
Submit
64. An individual heterogeneous nuclear RNA (hnRNA)
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65. The RNA Polymerase SLOWS down in
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66. A STRONG promoter is one that
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67. In the RELAXED "B" DNA, one of these group of numbers is CORRECT:
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68. The Klenow fragment of E. coli DNA polymerase I can be used to add nucleotides to a DNA with staggered ends if the ends...
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69. TRANSCRIPTION is throught to generate
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70. The following are part of DNA REPLICATION in E. coli, EXCEPT
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71. Telomerase is an enzyme that (p 1008)
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72. Which statement is incorrect about a typical E. coli promoter?
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73. E. coli DNA POLYMERASE I has all of the following characteristics EXCEPT
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74. The genes for the enzymes needed int he synthetic pathway of a biomolecule usually are found int he form of
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75. The POLYMERASE IN EUKARYOTIC REPLICATION which plays a similar role as that of PROKARYOTIC'S Polymerase III is?
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76. The SITE  where RNA polymerase BINDS is the
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77. The COMMON activities of DNA pol I and pol III are:
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78. Which one of the following DNA sequences is palindromic?
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79. Which statement is INCORRECT about E. coli DNA polymerase I? (pg 991)
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80. The oligonucleotide, AUGGp, has all these properties, EXCEPT
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81. Since the HEPTAPEPTIDE repeats (in the carboxy terminal domain located in the large subunit of RNA Polymerase II) can be phosphorylated, the repeats must contain:
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82. Which statement is INCORRECT about E. coli DNA ligase?
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83. LEUCINE is coded by 6 different codons, this represents
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84. If snake venon phosphodiesterase (3' - "a" cutter) cleaved the molecule above, what fragments would you expect as a result
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85. T-RNA (Met) and t-RNA (fMet)
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86. Which is not a property of E.coli DNA polymerase III?
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87. The term "upstream" means (p 1019)
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88. Which of the following IS NOT a characteristic of REPLICATION of the E.coli CIRCULAR chromosome?
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89. Which of the following statements is incorrect when dsDNA is denatured? (pg 371-2)
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90. The KLENOW fragment has the following activities
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91. The Klenow fragment from E.coli DNA polymerase contains all of the following except (pg 993)
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92. This ENZYME carries ITS OWN PRIMER
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93. In dsDNA the direction of the strand is
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94. Under which of the following conditions does the melting temperature for the denaturation of duplex DNA decrease?
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95. In EUKARYOTES, mRNA is made by
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96. Which of the following factors or subunits is contained within the core RNA polymerase in prokaryotic organisms? (pg 1016)
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97. Prokaryotic genes have conserved DNA sequences centered around -10 and -35 before the initiation point of transcription.  THese regions contain a binding site for:
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98. The reason why DNA does not contain uracyl is because
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99. What statement is INCORRECT about the different RNA molecules?
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100. One of these codons is likely to code for NON-POLAR amino acid
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101. In eukaryotic translation
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102. Which statement is CORRECT about  RNA polymerase in E. coli?
Submit
103. In the processing of the Okazaki fragments into a continuous DNA strand in E. coli, which of the following enzyme activity IS NOT required?
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104. Which of the following double-stranded DNAs may convert to a cruciform structure?
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105. In the Sanger's DNA sequencing method of the following oligonucleotide, what will be the sequence read form the gel? AATCGATTCGGGCTATA.  The primer is 4 nucleotides long. (pg 359)
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106. The enzyme which REMOVES the RNA PRIMER from the OKAZAKI fragment is:
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107. The "PROOF-READER" activitiy of Pol I is its
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108. An enzyme that changes the shape of supercoiled DNA without changing its linking number is (pg 376 or 576)
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109. A strain of E. coli produces the same high level of beta-galactosidase regardless of the concentration of lactose in the medium.  This strain most liekly contains a deletion mutation in the gene (Note: you may assume that the deletion mutation causes loss of normal function) (pg 10)
Submit
110. DNA-DEPENDENT RNA polymerase acts on the DNA template in EUKARYOTIC CELLS in the SYNTHESIS of:
Submit
111. The lac operon includes (pg 1031)
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112. Which statemnt is INCORRECT aobut enhancer sequences in eukaryotes (pg 1015)
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113. Which statement is INCORRECT about a typical E. coli promoter? (pg 1019)
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114. Which polymerase has the highest processivity?
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115. The promoter site is
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116. The synthesis of the DNA strand is thermodynamically driven by
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117. RNA polymerase II (pg 102)
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118. Which statement is INCORRECT about RNA polymerase in E. coli?
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119. The subunit involved in melting dsDNA in RNA polymerase is
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120. Which E. coli DNA polymerase is responsible for the repair of DNA?
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121. TRANSCRIPTION is finished by one of these subunits ***
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122. Which of the following double-stranded DNAs may convert to a cruciform structure?
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123. The subunit in E. coli RNA polymerase which is required fro recognition of the promoter sequence is
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How many helical turns (the linking number) are found in a relaxed...
The linking number of a highly SUPERCOILED plasmid can be readily...
In duplex DNA, how many hydrogen bonds does thymine use?
The nucleic acid that is unstable in ACID is
A feature which is distinctive to Z-DNA is
Which of the following characteristic IS NOT associated with E. coli...
Which statement is INCORRECT about eh synthesis of RNA by RNA...
A DNA is found to exhibit a melting temperature of 75 C in 0.1 M NaCl....
Which one of the following characteristics IS NOT associated with E....
If you found a DNA molecule containing URACYL, the most probably CAUSE...
When DNA is placed in dilute acid, it gets
The molecule that carries the pure genetic message in eukaryotes is:
A feature which is distinctive to Z-DNA is
The polymerase which plays a similar role as that of Prokaryotic Pol...
Which item of experimental evidence gives the most support to the...
The component (s) of DNA which is responsible for the absorbanc band...
All are true of the DNA double helix EXCEPT:
The RNA molecules which are involved in PROCESSING the hnRNA are
A strain of E.coli produces the same high level of beta-galactosidase...
In the repair of DNA, the E.coli DNA polymerase involved performs...
Which statement is INCORRECT about the STRUCTURE of native E. COLI...
Why is a primer strand needed in DNA replication?
E. coli replication on the lagging strand (pg 1002)
DNA pol III  REPLICATES the LAGGING strand by using
E. coli REPLICATION on the LAGGING strand
DOUBLE-stranded RNA is found in?
The linking number of a highly supercoiled plasmid can be readily...
Which one of the following characteristics ls not associated with...
Which IS NOT a property of E. coli DNA Polymerase III?
The Pribnow box is located
In eukaryotes, there are many MORE PROTEINS than GENES.  This can...
The KLENOW fragment of E. Coli DNA Polymerase I can be used to ADD...
In eukaryotic cells, the only RNA molecule which has a poly (A) tail...
A DNA is found to exhibit a melting temperature of 75 degrees Celsius...
INCREASE in gene expression in response to a METABOLITE is called:
A HELICASE is characterized by:
Which statement is INCORRECT about E. coli DNA Polymerase I?
IN THE REPAIR of DNA, the E.coli DNA POLYMERASE involved performs...
One of the following is used for protein synthesis:
In order to ACTIVATE the catabolite activator protein (CAP), it has to...
Telomerase is found in humans in
The reason why DNA is stable in BASE and RNA is not is because
What is the primary role of the template strand in DNA replication?
Why is a primer strand needed in DNA replication?
Which E. coli ENZYME has the GREATEST FREQUENCY of incorporating the...
The TUS protein is a type of
A GENE is a DNA SEQUENCE that encodes for:
E. coli DNA polymerase I has all of the following characteristics...
POSITIVE control of the LAC operon is ACHIEVED by:
Which E. coli enzyme has the greatest frequency of incorporating the...
Pure DNA has an absorbance ratio of:
Which of the following characteristics is associated with the B form...
Which of the following IS NOT a characteristic for DNA synthesis?
To what target sequence on DNA is the oligonucleotide pAATTCCGG...
The RNA molecules which are involved in processing the hnRNA are
Which of the following is not a characteristic for DNA synthesis?
Which of the following enzymes possesses a 5' to 3'...
In eukaryotes, a DNA is found in:
The CLOSE promoter COMPLEX is
Partial digestion of the oligonucleotide d-AGTACAA by pancreatic...
In the Sanger's DNA sequencing method, what is the reason for the...
In the repair of DNA, the E.coli DNA polymerase invovled performs...
Which statement is INCORRECT about the structure of native E.coli...
An individual heterogeneous nuclear RNA (hnRNA)
The RNA Polymerase SLOWS down in
A STRONG promoter is one that
In the RELAXED "B" DNA, one of these group of numbers is...
The Klenow fragment of E. coli DNA polymerase I can be used to add...
TRANSCRIPTION is throught to generate
The following are part of DNA REPLICATION in E. coli, EXCEPT
Telomerase is an enzyme that (p 1008)
Which statement is incorrect about a typical E. coli promoter?
E. coli DNA POLYMERASE I has all of the following characteristics...
The genes for the enzymes needed int he synthetic pathway of a...
The POLYMERASE IN EUKARYOTIC REPLICATION which plays a similar role as...
The SITE  where RNA polymerase BINDS is the
The COMMON activities of DNA pol I and pol III are:
Which one of the following DNA sequences is palindromic?
Which statement is INCORRECT about E. coli DNA polymerase I? (pg...
The oligonucleotide, AUGGp, has all these properties, EXCEPT
Since the HEPTAPEPTIDE repeats (in the carboxy terminal domain located...
Which statement is INCORRECT about E. coli DNA ligase?
LEUCINE is coded by 6 different codons, this represents
If snake venon phosphodiesterase (3' - "a" cutter)...
T-RNA (Met) and t-RNA (fMet)
Which is not a property of E.coli DNA polymerase III?
The term "upstream" means (p 1019)
Which of the following IS NOT a characteristic of REPLICATION of the...
Which of the following statements is incorrect when dsDNA is...
The KLENOW fragment has the following activities
The Klenow fragment from E.coli DNA polymerase contains all of the...
This ENZYME carries ITS OWN PRIMER
In dsDNA the direction of the strand is
Under which of the following conditions does the melting temperature...
In EUKARYOTES, mRNA is made by
Which of the following factors or subunits is contained within the...
Prokaryotic genes have conserved DNA sequences centered around -10 and...
The reason why DNA does not contain uracyl is because
What statement is INCORRECT about the different RNA molecules?
One of these codons is likely to code for NON-POLAR amino acid
In eukaryotic translation
Which statement is CORRECT about  RNA polymerase in E. coli?
In the processing of the Okazaki fragments into a continuous DNA...
Which of the following double-stranded DNAs may convert to a cruciform...
In the Sanger's DNA sequencing method of the following...
The enzyme which REMOVES the RNA PRIMER from the OKAZAKI fragment is:
The "PROOF-READER" activitiy of Pol I is its
An enzyme that changes the shape of supercoiled DNA without changing...
A strain of E. coli produces the same high level of beta-galactosidase...
DNA-DEPENDENT RNA polymerase acts on the DNA template in EUKARYOTIC...
The lac operon includes (pg 1031)
Which statemnt is INCORRECT aobut enhancer sequences in eukaryotes (pg...
Which statement is INCORRECT about a typical E. coli promoter? (pg...
Which polymerase has the highest processivity?
The promoter site is
The synthesis of the DNA strand is thermodynamically driven by
RNA polymerase II (pg 102)
Which statement is INCORRECT about RNA polymerase in E. coli?
The subunit involved in melting dsDNA in RNA polymerase is
Which E. coli DNA polymerase is responsible for the repair of DNA?
TRANSCRIPTION is finished by one of these subunits ***
Which of the following double-stranded DNAs may convert to a cruciform...
The subunit in E. coli RNA polymerase which is required fro...
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