Bio1339 Cells - Techniques In Cell Biology - Lecture Two

The resolution of a microscope refers to its ability to distinguish two closely spaced objects as separate entities. Smaller wavelength of light means that the light waves are closer together, allowing for better resolution. This is because smaller wavelengths can detect smaller details and provide a clearer image. Therefore, the statement "Smaller wavelength = better resolution" is true for microscopes.

The optical resolution limit refers to the minimum distance required for recognizing object details. This limit is determined by the capabilities of the optical system, such as the lens and sensor, to accurately capture and distinguish fine details. It signifies the point at which further reduction in distance would result in a loss of clarity and recognition of object features.