Principles And Processes Of Biotechnology

15 Questions

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Biotechnology Quizzes & Trivia

Time: 30 Minute


Questions and Answers
  • 1. 
    The enzyme that cuts specifically recognition sites in DNA is known as:
    • A. 

      DNA ligase

    • B. 

      DNA polymerase

    • C. 

      Reverse transcriptase

    • D. 

      Restriction endonuclease

  • 2. 
    Genetic engineering is possible because:
    • A. 

      Phenomenon of transduction in bacteria is understood

    • B. 

      We can see DNA by electron microscope

    • C. 

      We can cut DNA at specific sites by endonuclease like DNAase I

    • D. 

      Restriction endonucleases purified from bacteria can be used in vitro

  • 3. 
    The chemical knives of DNAs are __________
    • A. 

      Ligases

    • B. 

      Polymerases

    • C. 

      Endonucleases

    • D. 

      Transcriptases

  • 4. 
    When the genotype of an organism is improved by the addition of foreign genes, the process is called:
    • A. 

      Biotechnology

    • B. 

      Tissue culture.

    • C. 

      Genetic engineering

    • D. 

      Genetic diversity

  • 5. 
    Recombinant DNA technology can be used to produce large quantities of biologically active form of which one of the following products in E. coli?
    • A. 

      Luteining hormone

    • B. 

      Ecdysone

    • C. 

      Rifamycin

    • D. 

      Interferon

  • 6. 
    One bacterium which has found extensive use in genetic engineering work in plants is:
    • A. 

      Xanthomonas citri

    • B. 

      Bacillus coagulens

    • C. 

      Clostridium septium

    • D. 

      Agrobacterium tumefaciens

  • 7. 
    Eco RI cleaves DNA at:
    • A. 

      AAGGTT

    • B. 

      GTATATC

    • C. 

      GAATTC

    • D. 

      TATAGC

  • 8. 
    The enzyme used in polymerase chain reaction is:
    • A. 

      Taq polymerase

    • B. 

      RNA polymerase

    • C. 

      Ribonuclease

    • D. 

      Endonuclease

  • 9. 
    Identify the plasmid from the following:
    • A. 

      Eco RI

    • B. 

      PBR 322

    • C. 

      AIUI

    • D. 

      Hind II

  • 10. 
    Restriction endonucleases are most widely used in recombinant DNA technology. They are obtained from:
    • A. 

      Bacteriophage

    • B. 

      Bacterial cell

    • C. 

      Plasmids

    • D. 

      All prokaryotic cells

  • 11. 
    Polymerase chain reaction is most useful in:
    • A. 

      DNA synthesis

    • B. 

      DNA amplification

    • C. 

      Protein synthesis

    • D. 

      Amino acid synthesis

  • 12. 
    Ligase is used for:
    • A. 

      Separating DNA

    • B. 

      Joining two DNA fragments

    • C. 

      DNA polymerase reaction

    • D. 

      All of these

  • 13. 
    Gel electrophoresis is used for:
    • A. 

      Isolation of DNA molecule

    • B. 

      Cutting DNA into fragments

    • C. 

      Separation of DNA fragments according to their size

    • D. 

      Construction of recombination DNA by joining with closing vectors

  • 14. 
    The linking of antibiotic gene with the plasmid vector became possible with:
    • A. 

      Exonuclease

    • B. 

      DNA ligase

    • C. 

      Endonucleases

    • D. 

      DNA polymerase

  • 15. 
    Which one do you like?
    • A. 

      Wilmut

    • B. 

      A. Jeffreys

    • C. 

      Kary Mullis

    • D. 

      Eithoven