Principles And Processes Of Biotechnology

15 Questions | By Jagranjosh | Updated: Mar 21, 2022 | Attempts: 3652

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Time: 30 Minute

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1.

The enzyme that cuts specifically recognition sites in DNA is known as:
- A.
  
  DNA ligase
- B.
  
  DNA polymerase
- C.
  
  Reverse transcriptase
- D.
  
  Restriction endonuclease
2.

Genetic engineering is possible because:
- A.
  
  Phenomenon of transduction in bacteria is understood
- B.
  
  We can see DNA by electron microscope
- C.
  
  We can cut DNA at specific sites by endonuclease like DNAase I
- D.
  
  Restriction endonucleases purified from bacteria can be used in vitro
3.

The chemical knives of DNAs are __________
- A.
  
  Ligases
- B.
  
  Polymerases
- C.
  
  Endonucleases
- D.
  
  Transcriptases
4.

When the genotype of an organism is improved by the addition of foreign genes, the process is called:
- A.
  
  Biotechnology
- B.
  
  Tissue culture.
- C.
  
  Genetic engineering
- D.
  
  Genetic diversity
5.

Identify the plasmid from the following:
- A.
  
  Eco RI
- B.
  
  PBR 322
- C.
  
  AIUI
- D.
  
  Hind II
6.

Recombinant DNA technology can be used to produce large quantities of biologically active form of which one of the following products in E. coli?
- A.
  
  Luteining hormone
- B.
  
  Ecdysone
- C.
  
  Rifamycin
- D.
  
  Interferon
7.

One bacterium which has found extensive use in genetic engineering work in plants is:
- A.
  
  Xanthomonas citri
- B.
  
  Bacillus coagulens
- C.
  
  Clostridium septium
- D.
  
  Agrobacterium tumefaciens
8.

Eco RI cleaves DNA at:
- A.
  
  AAGGTT
- B.
  
  GTATATC
- C.
  
  GAATTC
- D.
  
  TATAGC
9.

The enzyme used in polymerase chain reaction is:
- A.
  
  Taq polymerase
- B.
  
  RNA polymerase
- C.
  
  Ribonuclease
- D.
  
  Endonuclease
10.

Restriction endonucleases are most widely used in recombinant DNA technology. They are obtained from:
- A.
  
  Bacteriophage
- B.
  
  Bacterial cell
- C.
  
  Plasmids
- D.
  
  All prokaryotic cells
11.

Polymerase chain reaction is most useful in:
- A.
  
  DNA synthesis
- B.
  
  DNA amplification
- C.
  
  Protein synthesis
- D.
  
  Amino acid synthesis
12.

Ligase is used for:
- A.
  
  Separating DNA
- B.
  
  Joining two DNA fragments
- C.
  
  DNA polymerase reaction
- D.
  
  All of these
13.

The linking of antibiotic gene with the plasmid vector became possible with:
- A.
  
  Exonuclease
- B.
  
  DNA ligase
- C.
  
  Endonucleases
- D.
  
  DNA polymerase
14.

Gel electrophoresis is used for:
- A.
  
  Isolation of DNA molecule
- B.
  
  Cutting DNA into fragments
- C.
  
  Separation of DNA fragments according to their size
- D.
  
  Construction of recombination DNA by joining with closing vectors
15.

Which one do you like?
- A.
  
  Wilmut
- B.
  
  A. Jeffreys
- C.
  
  Kary Mullis
- D.
  
  Eithoven