Principles And Processes Of Biotechnology
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Time: 30 Minute
- 1.The enzyme that cuts specifically recognition sites in DNA is known as:
- A.
DNA ligase
- B.
DNA polymerase
- C.
Reverse transcriptase
- D.
Restriction endonuclease
- 2.Genetic engineering is possible because:
- A.
Phenomenon of transduction in bacteria is understood
- B.
We can see DNA by electron microscope
- C.
We can cut DNA at specific sites by endonuclease like DNAase I
- D.
Restriction endonucleases purified from bacteria can be used in vitro
- 3.The chemical knives of DNAs are __________
- A.
Ligases
- B.
Polymerases
- C.
Endonucleases
- D.
Transcriptases
- 4.When the genotype of an organism is improved by the addition of foreign genes, the process is called:
- A.
Biotechnology
- B.
Tissue culture.
- C.
Genetic engineering
- D.
Genetic diversity
- 5.Recombinant DNA technology can be used to produce large quantities of biologically active form of which one of the following products in E. coli?
- A.
Luteining hormone
- B.
Ecdysone
- C.
Rifamycin
- D.
Interferon
- 6.One bacterium which has found extensive use in genetic engineering work in plants is:
- A.
Xanthomonas citri
- B.
Bacillus coagulens
- C.
Clostridium septium
- D.
Agrobacterium tumefaciens
- 7.Eco RI cleaves DNA at:
- A.
AAGGTT
- B.
GTATATC
- C.
GAATTC
- D.
TATAGC
- 8.The enzyme used in polymerase chain reaction is:
- A.
Taq polymerase
- B.
RNA polymerase
- C.
Ribonuclease
- D.
Endonuclease
- 9.Identify the plasmid from the following:
- A.
Eco RI
- B.
PBR 322
- C.
AIUI
- D.
Hind II
- 10.Restriction endonucleases are most widely used in recombinant DNA technology. They are obtained from:
- A.
Bacteriophage
- B.
Bacterial cell
- C.
Plasmids
- D.
All prokaryotic cells
- 11.Polymerase chain reaction is most useful in:
- A.
DNA synthesis
- B.
DNA amplification
- C.
Protein synthesis
- D.
Amino acid synthesis
- 12.Ligase is used for:
- A.
Separating DNA
- B.
Joining two DNA fragments
- C.
DNA polymerase reaction
- D.
All of these
- 13.Gel electrophoresis is used for:
- A.
Isolation of DNA molecule
- B.
Cutting DNA into fragments
- C.
Separation of DNA fragments according to their size
- D.
Construction of recombination DNA by joining with closing vectors
- 14.The linking of antibiotic gene with the plasmid vector became possible with:
- A.
Exonuclease
- B.
DNA ligase
- C.
Endonucleases
- D.
DNA polymerase
- 15.Which one do you like?
- A.
Wilmut
- B.
A. Jeffreys
- C.
Kary Mullis
- D.
Eithoven
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