Time: 30 Minute
DNA ligase
DNA polymerase
Reverse transcriptase
Restriction endonuclease
Phenomenon of transduction in bacteria is understood
We can see DNA by electron microscope
We can cut DNA at specific sites by endonuclease like DNAase I
Restriction endonucleases purified from bacteria can be used in vitro
Ligases
Polymerases
Endonucleases
Transcriptases
Biotechnology
Tissue culture.
Genetic engineering
Genetic diversity
Eco RI
PBR 322
AIUI
Hind II
Luteining hormone
Ecdysone
Rifamycin
Interferon
Xanthomonas citri
Bacillus coagulens
Clostridium septium
Agrobacterium tumefaciens
AAGGTT
GTATATC
GAATTC
TATAGC
Taq polymerase
RNA polymerase
Ribonuclease
Endonuclease
Bacteriophage
Bacterial cell
Plasmids
All prokaryotic cells
DNA synthesis
DNA amplification
Protein synthesis
Amino acid synthesis
Separating DNA
Joining two DNA fragments
DNA polymerase reaction
All of these
Exonuclease
DNA ligase
Endonucleases
DNA polymerase
Isolation of DNA molecule
Cutting DNA into fragments
Separation of DNA fragments according to their size
Construction of recombination DNA by joining with closing vectors
Wilmut
A. Jeffreys
Kary Mullis
Eithoven
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