The 'DNA Biotechnology Sample Questions' quiz assesses understanding of key biotechnological methods involving DNA manipulation. Topics include restriction endonucleases, plasmids, DNA fingerprinting, northern blot analysis, and genetic disease detection, emphasizing practical applications in medicine and research.
Histocompatibility loci
Centromeres
Microsatellite tandem repeats (STRs)
Restriction enzyme sites
Single-copy sequences
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They always yield overhanging single-stranded ends
They recognize methylated DNA sequences
They recognize triplet repeats
They cleave both strands of duplex DNA
They always yield blunt ends
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They are found more commonly in viruses than in bacteria
They are single-stranded circles
They sometimes enhance bacterial susceptibility to antibiotics
They sometimes enhance bacterial resistance to antibiotics
They are too small to be useful as vectors for the cloning of mammalian DNA segments
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DNA can be isolated from blood, skin, or sperm and analyzed for variable patterns of restriction fragments arising from tandemly repeated sequences (microsatellites)
DNA is copied from blood, skin, or sperm RNA using reverse transcriptase and analyzed for patterns of complementary DNAs
DNA is isolated from blood, skin, or sperm and its fragment size distribution is analyzed by gel electrophorsis
DNA is isolated from blood, skin, or sperm and hybridized with probes from the HLA locus to visualize HLA gene patterns
DNA is isolated from blood, skin, or sperm, centrifuged to separate satellite DNA fractions, and analyzed by gel electrophoresis.
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Isolation of DNA from red blood cells followed by polymerase chain reaction (PCR) amplification and restriction enzyme digestion.
Isolation of DNA from blood leukocytes followed by Southern blot analysis to detect globin gene exon sizes
Isolation of DNA from blood leukocytes followed by DNA sequencing of globin gene introns
Isolation of DNA from blood leukocytes followed by polymerase chain reaction (PCR) amplification and allele-specific oligonucleotide (ASO) hybridization
Western blot analysis of red blood cell extracts
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CCTCACCTCAGG
CCTGTGGAGAAG
GGACACCTCTTC
CTTCTCCACAGG
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GAGTCCCTCAAGTCCTTC and CGGCGGTGGCGGCTGTTG
CTTCCTGAACTCCCTGAG and CAACAGCCGCCACCGCCG
CTTCCTGAACTCCCTGAG and CGGCGGTGGCGGCTGTTG
GAGTCCCTCAAGTCCTTC and CAACAGCCGCCACCGCCG
GAGTCCCTCAAGTCCTTC and GTTGTCGGCGGTGGCGGC
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