Wheat/Human DNA Extraction Test

35 Questions | Total Attempts: 171

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Wheat/Human DNA Extraction Test

Welcome to the electronic frontier classroom of the 21st century. This testing instrument deals with the simple extraction of DNA from wheat germ and attempts of human DNA. You will find additional information on my science web site @ Mitchell's Cosmic Adventure. I hope your biology instructor will allow you the chance to complete an activity on extraction and observation of DNA.


Questions and Answers
  • 1. 
    Which substance is added first to the wheat germ?
    • A. 

      Detergent

    • B. 

      Oil

    • C. 

      Salt

    • D. 

      Water

  • 2. 
    Which step did you add the detergent?
    • A. 

      1

    • B. 

      2

    • C. 

      3

    • D. 

      4

    • E. 

      5

  • 3. 
    How many drops of salt solution was used for this experiment?
    • A. 

      5 - 10

    • B. 

      15 - 20

    • C. 

      25 - 30

    • D. 

      35 - 40

    • E. 

      45 - 50

  • 4. 
    The increase number of bubbles produces ____ DNA amount from the wheat germ.
    • A. 

      Decrease

    • B. 

      Increase

    • C. 

      No DNA

  • 5. 
    How many minutes do you swirl the test tube after detergent was added?
    • A. 

      1

    • B. 

      3

    • C. 

      5

    • D. 

      8

  • 6. 
    How many minutes do you swirl the test tube after the addition of salt?
    • A. 

      1

    • B. 

      2

    • C. 

      3

    • D. 

      5

  • 7. 
    The DNA can be extracted from the layer between wheat germ and alcohol.
    • A. 

      True

    • B. 

      False

  • 8. 
    Which cellular organelles were disrupted by the addition of detergent?
    • A. 

      Ribosomes and Plasma Membrane

    • B. 

      Plasma Membrane

    • C. 

      Nuclear and Plasma Membranes

    • D. 

      Only Nuclear Membrane

    • E. 

      None of the above are correct answers

  • 9. 
    The DNA molecules travel away from the alcohol layer.
    • A. 

      True

    • B. 

      False

  • 10. 
    After adding 25 - 30 mL of detergent. You gently shook the mixture.
    • A. 

      True

    • B. 

      False

  • 11. 
    You cleaned up your "work area" with soapy water and paper towels.
    • A. 

      True

    • B. 

      False

  • 12. 
    Which kingdom does the wheat belong too?
    • A. 

      Animalia

    • B. 

      Fungi

    • C. 

      Plantae

    • D. 

      Protista

  • 13. 
    Which chemical substance was it's volume doubled the total amount in the test tube?
    • A. 

      Alcohol

    • B. 

      Detergent

    • C. 

      Salt

    • D. 

      Wheat Germ

  • 14. 
    Where is the DNA located in the wheat germ cell? (please use all lower case letters when entering your answer for this question.)
  • 15. 
    Which substance was added first to the wheat germ placed into the test tube?
    • A. 

      Cold ethyl alcohol

    • B. 

      Cold isopropyl alcohol

    • C. 

      Warm Detergent

    • D. 

      Warm Water

  • 16. 
    How did you add the isopropyl alcohol to the test tube?
    • A. 

      Fast pour

    • B. 

      Slowly to the test tube straight up

    • C. 

      Test tube tilted at the angle

    • D. 

      Did not add alcohol to the mixture

  • 17. 
    A bent paper clip was used in your experiment to collect a DNA sample.
    • A. 

      True

    • B. 

      False

  • 18. 
    Which of the substances used in this experiment has the lowest density?
    • A. 

      Alcohol

    • B. 

      Salt

    • C. 

      Warm Water

  • 19. 
    How many strands make up a DNA molecule?
    • A. 

      1

    • B. 

      2

    • C. 

      4

  • 20. 
    DNA from just one wheat germ cell is visible without the use of a lens or microscopy.
    • A. 

      True

    • B. 

      False

  • 21. 
    If you used toasted wheat germ in this investigation.  You would have gotten more DNA in the test tube.
    • A. 

      True

    • B. 

      False

  • 22. 
    A light microscope of 2,000 X is strong enough to see individual DNA molecules.
    • A. 

      True

    • B. 

      False

  • 23. 
    What is the correct order of adding chemicals to the wheat germ?
    • A. 

      Water - Alcohol - Salt - Detergent

    • B. 

      Detergent - Alcohol - Salt - Water

    • C. 

      Water - Detergent - Salt - Alcohol

  • 24. 
    If you added alcohol - water - detergent - salt in this order.  DNA would still form inside of the test tube.
    • A. 

      True

    • B. 

      False

  • 25. 
    Forensic scientists use DNA to solve crime scene investigations.
    • A. 

      True

    • B. 

      False