DNA Extraction Warm Up 1

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| By John Mitchell
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John Mitchell
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Quizzes Created: 100 | Total Attempts: 135,992
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DNA Extraction Warm Up 1 - Quiz

Welcome to the electronic biology classroom of the 21st Century. This biology warm-up or bell ringer concentrates on the extraction and observing of DNA taken from wheat germ, strawberry and human epithelial tissues. I hope you read the EXPERIMENT: DNA Extraction Techniques!
This laboratory warm up will permit you unlimited number of attempts to improve your test-taking and learning skills in advanced biology. As a bonus, each attempt will randomly generate new questions and answers.


Questions and Answers
  • 1. 

    How many diploid chromosomes does wheat (Triticum aestivum)have in it's nucleus during in the resting stage?

    • A.

      7

    • B.

      14

    • C.

      21

    • D.

      42

    • E.

      56

    Correct Answer
    D. 42
    Explanation
    Wheat (Triticum aestivum) has 42 diploid chromosomes in its nucleus during the resting stage. Diploid refers to the number of chromosomes in a cell, where each chromosome is present in pairs. In the case of wheat, there are 21 pairs of chromosomes, resulting in a total of 42 chromosomes.

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  • 2. 

    How many sets of chromosomes can strawberries have in their nucleus?

    • A.

      8

    • B.

      50

    • C.

      240

    • D.

      360

    Correct Answer
    A. 8
    Explanation
    Strawberries typically have 8 sets of chromosomes in their nucleus. This means that they are octoploid, with each set of chromosomes consisting of 8 chromosomes. This is in contrast to humans, who are diploid and have 2 sets of chromosomes. The number of sets of chromosomes in an organism's nucleus determines its ploidy level, which can vary among different species.

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  • 3. 

    What chemical is added to the wheat germ after preparing the test tube?

    • A.

      Isopropyl alcohol

    • B.

      Detergent solution

    • C.

      Distilled water

    • D.

      DNA Helicase

    • E.

      Sodium Chloride solution

    Correct Answer
    C. Distilled water
    Explanation
    After preparing the test tube, distilled water is added to the wheat germ. Distilled water is used because it is free from impurities and contaminants. It serves as a control in the experiment, allowing for accurate comparison and measurement of the effects of other substances added to the wheat germ.

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  • 4. 

    What chemical is added to the wheat germ and distilled water?

    • A.

      Isopropyl alcohol

    • B.

      Detergent solution

    • C.

      Distilled water

    • D.

      DNA Helicase

    • E.

      Sodium Chloride solution

    Correct Answer
    B. Detergent solution
    Explanation
    The correct answer is detergent solution. Detergent solution is added to the wheat germ and distilled water in order to break down the cell membranes and release the DNA. Detergents are able to disrupt the lipid bilayer of the cell membrane, allowing the DNA to be extracted.

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  • 5. 

    What chemical is added to the wheat germ, distilled water, detergent and sodium chloride solution?

    • A.

      Isopropyl alcohol

    • B.

      Detergent solution

    • C.

      Distilled water

    • D.

      DNA Helicase

    • E.

      Sodium Chloride solution

    Correct Answer
    A. Isopropyl alcohol
    Explanation
    Isopropyl alcohol is added to the wheat germ, distilled water, detergent, and sodium chloride solution. This chemical is commonly used as a solvent and disinfectant. It is likely added to the solution to sterilize the equipment or to extract certain compounds from the mixture. Isopropyl alcohol has a low boiling point, which makes it easy to evaporate and leave behind the desired components.

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  • 6. 

    What chemical is added to the wheat germ, distilled water, detergent and sodium chloride solution. What layer should be on the top of the column in the test tube?

    • A.

      Isopropyl alcohol

    • B.

      Detergent solution

    • C.

      Distilled water

    • D.

      DNA Helicase

    • E.

      Sodium Chloride solution

    Correct Answer
    A. Isopropyl alcohol
    Explanation
    Isopropyl alcohol should be added to the mixture of wheat germ, distilled water, detergent, and sodium chloride solution. The layer of isopropyl alcohol should be on the top of the column in the test tube.

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  • 7. 

    What chemical is added to the wheat germ, distilled water, detergent and sodium chloride solution. What layer should be in the middle layer of the column in the test tube?

    • A.

      Isopropyl alcohol

    • B.

      Detergent solution

    • C.

      Distilled water

    • D.

      Extracted DNA

    • E.

      Sodium Chloride solution

    Correct Answer
    D. Extracted DNA
    Explanation
    The correct answer is "Extracted DNA" because the question is asking for the chemical that is added to the mixture of wheat germ, distilled water, detergent, and sodium chloride solution. The extracted DNA is the chemical that is added to the mixture to observe its effects or analyze it further. The other options mentioned are not chemicals that are added to the mixture but rather components or solutions that are already present in the experiment.

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  • 8. 

    What chemical is added to the wheat germ, distilled water, detergent and sodium chloride solution. What layer should be in the bottom  of the column in the test tube?

    • A.

      Isopropyl alcohol

    • B.

      Detergent solution

    • C.

      Distilled water

    • D.

      Extracted DNA

    • E.

      Sodium Chloride solution

    • F.

      Wheat germ

    Correct Answer
    F. Wheat germ
    Explanation
    In this experiment, various chemicals are added to the test tube, including wheat germ, distilled water, detergent solution, and sodium chloride solution. The question asks which layer should be in the bottom of the column in the test tube. Since the question does not specify any specific layers or column, it is difficult to determine the exact answer. However, based on the given options, it can be inferred that the wheat germ is the correct answer because it is the only option that is not a solution or a chemical, but rather a solid substance.

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  • 9. 

    Which chemicals should be mixed into the "wheat germ" layer?  (HINT: there are multiple answers for this question.)

    • A.

      Distilled water

    • B.

      Sodium Chloride solution

    • C.

      Detergent solution

    • D.

      Isopropyl alcohol

    • E.

      Strawberry Extracted DNA

    Correct Answer(s)
    A. Distilled water
    B. Sodium Chloride solution
    C. Detergent solution
    Explanation
    The chemicals that should be mixed into the "wheat germ" layer are distilled water, sodium chloride solution, and detergent solution. These chemicals are commonly used in DNA extraction protocols. Distilled water is used as a solvent to dissolve other substances. Sodium chloride solution is used to create a hypertonic environment, which helps to separate DNA from other cellular components. Detergent solution is used to break down the cell membranes and release the DNA. Therefore, mixing these chemicals into the "wheat germ" layer would facilitate the extraction of DNA from wheat germ.

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  • 10. 

    Describe the temperature of the isopropyl alcohol at the time mixing it into the test tube.

    • A.

      Room Temperature

    • B.

      Warm

    • C.

      Hot

    • D.

      Cold

    • E.

      Extremely Cold

    Correct Answer
    E. Extremely Cold
    Explanation
    The isopropyl alcohol is described as "extremely cold" when it is mixed into the test tube. This suggests that the temperature of the isopropyl alcohol is significantly lower than room temperature, warm, hot, or cold. The term "extremely cold" implies that the isopropyl alcohol is at a very low temperature, possibly close to or below freezing point.

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  • Current Version
  • Mar 22, 2023
    Quiz Edited by
    ProProfs Editorial Team
  • Mar 23, 2012
    Quiz Created by
    John Mitchell

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