Basic Microbiology Quiz

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Basic Microbiology Quiz - Quiz

Microbiology is the study of microbes; this informative quiz on basic Microbiology aims to test your knowledge about this vast field. Microbiology is one of the subjects that has gained a lot of traction over the past few years, and students are enrolling in the subject. The quiz contains various questions ranging from easy, medium, and hard levels that cover the theoretical and practical aspects of the subject. If you find this quiz helpful, do share it with others. All the best!


Questions and Answers
  • 1. 

    A respiratory culture usually uses which plates?

    • A.

      BAP,CHOC,MAC

    • B.

      BAP, CHOC, MAC, CNA, THIO

    • C.

      BAP, CNA, MAC

    • D.

      CNA, THIO, KV LAKED, MANNITOL

    Correct Answer
    B. BAP, CHOC, MAC, CNA, THIO
    Explanation
    A respiratory culture usually uses BAP (Blood Agar Plate), CHOC (Chocolate Agar Plate), MAC (MacConkey Agar Plate), CNA (Columbia CNA Agar Plate), and THIO (Thioglycollate Agar Plate). These plates are commonly used to culture and identify bacteria and other microorganisms present in respiratory samples. BAP supports the growth of most bacteria and helps in identifying different types of organisms. CHOC agar is used to culture fastidious organisms like Haemophilus influenzae. MAC agar is selective for gram-negative bacteria. CNA agar is selective for gram-positive bacteria. THIO agar is used to culture anaerobic bacteria.

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  • 2. 

    A Strep Screen usually uses which plates?

    • A.

      BAP, or CNA, or SXT

    • B.

      BAP only

    • C.

      CHOC

    • D.

      BAP, MAC, CNA

    Correct Answer
    A. BAP, or CNA, or SXT
    Explanation
    A Strep Screen usually uses BAP (Blood Agar Plate), CNA (Colistin Nalidixic Acid Agar), or SXT (Sulfamethoxazole-Trimethoprim Agar) plates. These plates are commonly used in clinical laboratories to isolate and identify Streptococcus species, which are known to cause various infections in humans. BAP is a general-purpose medium that supports the growth of most bacteria, while CNA is selective for Gram-positive bacteria, including Streptococcus. SXT agar is used to detect the presence of Group B Streptococcus in pregnant women.

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  • 3. 

    A stool cultures usually uses which plates?

    • A.

      BAP, CHOC, CNA, MAC

    • B.

      BAP, CNA, MAC, MAC w/SORB

    • C.

      BAP, CNA, MAC, MAC w/SORB, HE

    • D.

      BAP, CNA, MAC, MAC w/SORB, HE, CHOC

    Correct Answer
    C. BAP, CNA, MAC, MAC w/SORB, HE
  • 4. 

    IMViC is the pneumonic for

    • A.

      Indole, Methyl Red, VP, Citrate

    • B.

      Indole, Methyl Red, VP, Catalase

    • C.

      Inducible, Methyl Red, VP, Citrate

    • D.

      Indole, Methyl Red, VP, Coagulase

    Correct Answer
    A. Indole, Methyl Red, VP, Citrate
    Explanation
    IMViC is a mnemonic used to remember a series of biochemical tests commonly used in microbiology to identify enteric bacteria. The correct answer, "Indole, Methyl Red, VP, Citrate", represents the four tests included in the IMViC series. Indole test is used to detect the production of indole from the breakdown of tryptophan, Methyl Red test is used to determine the ability of an organism to perform mixed acid fermentation, Voges-Proskauer (VP) test is used to detect the production of acetoin, and Citrate test is used to determine the ability of an organism to utilize citrate as a sole carbon source.

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  • 5. 

    What organism is IMViC ++--

    • A.

      E. Coli

    • B.

      PsA

    • C.

      Staph Aureus

    • D.

      Citrobactor

    Correct Answer
    A. E. Coli
    Explanation
    The IMViC test is a series of four biochemical tests used to identify bacteria in the Enterobacteriaceae family. The letters in IMViC stand for the tests: Indole, Methyl Red, Voges-Proskauer, and Citrate. The result "++--" indicates that the organism is positive for the Indole test, negative for the Methyl Red test, negative for the Voges-Proskauer test, and negative for the Citrate test. Among the given options, E. Coli is the only organism known to produce these specific results in the IMViC test.

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  • 6. 

    What organism is IMViC - + - -

    • A.

      E. Coli

    • B.

      PsA

    • C.

      Shigella

    • D.

      Citrobactor

    Correct Answer
    C. Shigella
    Explanation
    The IMViC test is a series of four biochemical tests used to differentiate between different species of bacteria. In this case, the result of IMViC - + - - suggests that the organism is Shigella. Each letter in IMViC represents a different test: I for indole production (negative), M for methyl red test (positive), V for Voges-Proskauer test (negative), and C for citrate utilization test (negative). Shigella is known to exhibit this specific combination of test results, leading to the conclusion that it is the correct answer.

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  • 7. 

    What organism is IMViC - + - +

    • A.

      E. Coli

    • B.

      PsA

    • C.

      Shigella

    • D.

      Salmonella

    Correct Answer
    D. Salmonella
    Explanation
    The IMViC test is a series of biochemical tests used to identify certain enteric bacteria. The pattern IMViC - + - + indicates that the organism is negative for indole production, positive for methyl red test, negative for Voges-Proskauer test, and positive for citrate utilization. Among the given options, Salmonella is the only organism that fits this pattern. E. Coli is positive for indole production, PsA is positive for Voges-Proskauer test, and Shigella is negative for both methyl red test and citrate utilization.

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  • 8. 

    What organism is IMViC - - + +

    • A.

      E. Coli

    • B.

      Klebsiella

    • C.

      Shigella

    • D.

      Salmonella

    Correct Answer
    B. Klebsiella
    Explanation
    The IMViC test is used to differentiate between different species of bacteria based on their metabolic characteristics. The + - - + pattern indicates that the organism is negative for indole production, positive for methyl red test, negative for Voges-Proskauer test, and positive for citrate utilization. Among the given options, Klebsiella is the only organism that matches this pattern. Therefore, the correct answer is Klebsiella.

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  • 9. 

    What organism is IMViC ++--

    • A.

      E. Coli

    • B.

      Proteus Vulgaris

    • C.

      Staph Aureus

    • D.

      Proteus Mirabilis

    Correct Answer
    B. Proteus Vulgaris
    Explanation
    The IMViC test is a series of tests used to differentiate between different species of bacteria. In this case, the answer "Proteus Vulgaris" is correct because it is the only organism listed that matches the IMViC pattern of ++--. E. Coli, Staph Aureus, and Proteus Mirabilis do not match this pattern.

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  • 10. 

    What organism is IMViC - + - +

    • A.

      E. Coli

    • B.

      Proteus Vulgaris

    • C.

      Staph Aureus

    • D.

      Proteus Mirabilis

    Correct Answer
    D. Proteus Mirabilis
    Explanation
    The IMViC test is a series of four tests used to identify certain bacteria, including Escherichia coli (E. coli), Proteus Vulgaris, Staphylococcus aureus (Staph Aureus), and Proteus Mirabilis. The results of the IMViC test for the given organism show a positive result for the Indole test (I), a negative result for the Methyl Red test (M), a positive result for the Voges-Proskauer test (V), and a negative result for the Citrate test (C). Based on these results, the correct answer is Proteus Mirabilis.

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  • 11. 

    What organism is IMViC - + - +

    • A.

      E. Coli

    • B.

      Proteus Vulgaris

    • C.

      Staph Aureus

    • D.

      Citrobactor

    Correct Answer
    D. Citrobactor
    Explanation
    The IMViC test is used to identify certain Enterobacteriaceae, a family of bacteria that includes Citrobacter. The test involves four different reactions: Indole production (I), Methyl Red test (M), Voges-Proskauer test (V), and Citrate utilization (C). The given result "IMViC - + - +" suggests that the organism is negative for indole production (I-), positive for methyl red test (M+), negative for Voges-Proskauer test (V-), and positive for citrate utilization (C+). Among the options provided, Citrobacter is the only organism that matches this pattern.

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  • 12. 

    What organism is IMViC - - + +

    • A.

      E. Coli

    • B.

      Enterobacter

    • C.

      Shigella

    • D.

      Salmonella

    Correct Answer
    B. Enterobacter
  • 13. 

    What does TSI agar mean?

    • A.

      Triphosphate iron

    • B.

      Triple sugar iron

    • C.

      Triple sucrose iron

    • D.

      Ten sugar ion

    Correct Answer
    B. Triple sugar iron
    Explanation
    TSI agar refers to triple sugar iron agar, which is a type of agar medium used in microbiology to differentiate and identify bacteria based on their ability to ferment sugars and produce hydrogen sulfide gas. The medium contains three sugars (glucose, lactose, and sucrose) and iron salts. Bacteria that can ferment any of these sugars produce acid, which causes a color change in the agar. Additionally, bacteria that can produce hydrogen sulfide gas will react with the iron salts, resulting in the formation of a black precipitate. Therefore, the correct answer is "triple sugar iron."

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  • 14. 

    What does TSI contain?

    • A.

      Phenol red, glucose, lactose, sucrose, iron

    • B.

      Glucose, lactose, sucrose, ions

    • C.

      Methyl red, glucose, lactose, sucrose

    • D.

      VP, glucose, lactose, sucrose, iron

    • E.

      A

    Correct Answer
    A. Phenol red, glucose, lactose, sucrose, iron
    Explanation
    TSI (Triple Sugar Iron) is a type of agar medium used in microbiology to differentiate bacteria based on their ability to ferment different sugars and produce hydrogen sulfide gas. The correct answer, "phenol red, glucose, lactose, sucrose, iron," refers to the components present in TSI. Phenol red is the pH indicator that turns yellow in the presence of acid produced during sugar fermentation. Glucose, lactose, and sucrose are the three sugars present in TSI, which bacteria can ferment. Iron is added to detect the production of hydrogen sulfide gas, which reacts with iron to form a black precipitate.

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  • 15. 

    What does this TSI Indicate

    • A.

      K/A-

    • B.

      K/A+

    • C.

      A/A-

    • D.

      A/A+

    Correct Answer
    A. K/A-
    Explanation
    The TSI indicates the presence of antibodies in the blood that are specific to the bacteria that causes Klebsiella pneumoniae. The "K" stands for Klebsiella and the "A-" indicates the presence of antibodies against it. This result suggests that the person has been exposed to or has an ongoing infection with Klebsiella pneumoniae.

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  • 16. 

    What does this TSI Indicate

    • A.

      A/A H2S negative with gas

    • B.

      K/A-

    • C.

      K/A+

    • D.

      A/A-

    Correct Answer
    C. K/A+
    Explanation
    The TSI (Triple Sugar Iron) test is used to differentiate bacteria based on their ability to ferment sugars and produce gas. The given answer "K/A+" indicates that the organism is capable of fermenting glucose (K) but not lactose or sucrose (A), and it produces gas (+). This suggests that the organism is a glucose fermenter and gas producer, but does not ferment lactose or sucrose.

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  • 17. 

    What does this TSI Indicate

    • A.

      K/A-

    • B.

      K/A+

    • C.

      A/A-

    • D.

      A/A+

    Correct Answer
    D. A/A+
    Explanation
    The TSI (Thyroid Stimulating Immunoglobulin) measures the presence of antibodies in the blood that stimulate the thyroid gland. In this case, an A/A+ result indicates a high level of thyroid-stimulating antibodies, suggesting that the individual may have Graves' disease, an autoimmune disorder that causes the thyroid gland to produce too much thyroid hormone.

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  • 18. 

    What does this TSI Indicate

    • A.

      K/A-

    • B.

      K/A+

    • C.

      A/A-

    • D.

      A/A+

    Correct Answer
    C. A/A-
    Explanation
    This TSI (Thyroid Stimulating Immunoglobulin) indicates that the patient has high levels of thyroid-stimulating antibodies, which can lead to an overactive thyroid gland. The "A/A-" indicates the presence of these antibodies, suggesting a potential diagnosis of Graves' disease, an autoimmune disorder that causes hyperthyroidism. This condition can result in symptoms such as weight loss, rapid heartbeat, and anxiety. Treatment options may include medication to suppress thyroid function or surgery to remove the thyroid gland.

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  • 19. 

    What does this TSI Indicate

    • A.

      K/A-

    • B.

      K/A+

    • C.

      A/A-

    • D.

      A/A H2S negative with gas

    Correct Answer
    D. A/A H2S negative with gas
    Explanation
    The TSI (Triple Sugar Iron) test is used to determine the ability of an organism to ferment sugars and produce gas, as well as its ability to produce hydrogen sulfide (H2S). In this case, the TSI indicates that the organism is able to ferment both glucose and lactose (A/A), does not produce hydrogen sulfide (H2S negative), and produces gas.

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  • 20. 

    What common organism(s) might this TSI indicate?

    • A.

      E. Coli,Enterobactor,Klebsiella

    • B.

      Shigella, Citrobacter,Providencia,Proteus,Salmonella,Yersinia

    • C.

      Salmonella, Citrobacter, or Edwardsiella

    • D.

      Serratia,E. Coli,Enterobacter,Klebsiella

    Correct Answer
    B. Shigella, Citrobacter,Providencia,Proteus,Salmonella,Yersinia
    Explanation
    The correct answer indicates that the TSI might indicate the presence of Shigella, Citrobacter, Providencia, Proteus, Salmonella, and Yersinia.

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  • 21. 

    What common organism(s) might this TSI indicate?

    • A.

      E. Coli,Enterobactor,Klebsiella

    • B.

      Shigella, Citorbacter,Providencia,Proteus,Salmonella,Yersinia

    • C.

      Salmonella, Citrobacter, or Edwardsiella

    • D.

      Serratia,E. Coli,Enterobacter,Klebsiella

    Correct Answer
    C. Salmonella, Citrobacter, or Edwardsiella
    Explanation
    This TSI indicates the presence of Salmonella, Citrobacter, or Edwardsiella.

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  • 22. 

    What common organism(s) might this TSI indicate?

    • A.

      E. Coli,Enterobactor,Klebsiella

    • B.

      Shigella, Citorbacter,Providencia,Proteus,Salmonella,Yersinia

    • C.

      Salmonella, Citrobacter, or Edwardsiella

    • D.

      Serratia,E. Coli,Enterobacter,Klebsiella

    Correct Answer
    D. Serratia,E. Coli,Enterobacter,Klebsiella
    Explanation
    This TSI indicates the presence of Serratia, E. Coli, Enterobacter, and Klebsiella.

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  • 23. 

    What common organism(s) might this TSI indicate?

    • A.

      Citrobacter,Salmonella, Arizonae,Proteus

    • B.

      Shigella, Citorbacter,Providencia,Proteus,Salmonella,Yersinia

    • C.

      Salmonella, Citrobacter, or Edwardsiella

    • D.

      Serratia,E. Coli,Enterobacter,Klebsiella

    Correct Answer
    A. Citrobacter,Salmonella, Arizonae,Proteus
    Explanation
    The TSI (Triple Sugar Iron) test is used to differentiate between different species of Enterobacteriaceae based on their ability to ferment sugars and produce gas. The given correct answer includes Citrobacter, Salmonella, Arizonae, and Proteus, which are all common organisms that can be indicated by the TSI test. These organisms are known to ferment sugars and produce gas, which is characteristic of a positive TSI reaction.

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  • 24. 

    What common organism(s) might this TSI indicate?

    • A.

      E. Coli,Enterobactor,Klebsiella

    • B.

      Shigella, Citorbacter,Providencia,Proteus,Salmonella,Yersinia

    • C.

      Salmonella, Citrobacter, or Edwardsiella

    • D.

      Serratia,E. Coli,Enterobacter,Klebsiella

    Correct Answer
    A. E. Coli,Enterobactor,Klebsiella
    Explanation
    This TSI indicates the presence of E. Coli, Enterobactor, and Klebsiella.

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  • 25. 

    True or False This is GPC CL

    • A.

      True

    • B.

      False

    Correct Answer
    B. False

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  • Current Version
  • Sep 04, 2023
    Quiz Edited by
    ProProfs Editorial Team
  • Mar 05, 2015
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