Basic Microbiology Quiz
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Microbiology is the study of microbes; this informative quiz on basic Microbiology aims to test your knowledge about this vast field. Microbiology is one of the subjects that has gained a lot of traction over the past few years, and students are enrolling in the subject. The quiz contains various questions ranging from easy, medium, and hard levels that cover the theoretical and practical aspects of the subject. If you find this quiz helpful, do share it with others. All the best!
Questions and Answers
- 1.
A respiratory culture usually uses which plates?
- A.
BAP,CHOC,MAC
- B.
BAP, CHOC, MAC, CNA, THIO
- C.
BAP, CNA, MAC
- D.
CNA, THIO, KV LAKED, MANNITOL
Correct Answer
B. BAP, CHOC, MAC, CNA, THIOExplanation
A respiratory culture usually uses BAP (Blood Agar Plate), CHOC (Chocolate Agar Plate), MAC (MacConkey Agar Plate), CNA (Columbia CNA Agar Plate), and THIO (Thioglycollate Agar Plate). These plates are commonly used to culture and identify bacteria and other microorganisms present in respiratory samples. BAP supports the growth of most bacteria and helps in identifying different types of organisms. CHOC agar is used to culture fastidious organisms like Haemophilus influenzae. MAC agar is selective for gram-negative bacteria. CNA agar is selective for gram-positive bacteria. THIO agar is used to culture anaerobic bacteria.Rate this question:
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- 2.
A Strep Screen usually uses which plates?
- A.
BAP, or CNA, or SXT
- B.
BAP only
- C.
CHOC
- D.
BAP, MAC, CNA
Correct Answer
A. BAP, or CNA, or SXTExplanation
A Strep Screen usually uses BAP (Blood Agar Plate), CNA (Colistin Nalidixic Acid Agar), or SXT (Sulfamethoxazole-Trimethoprim Agar) plates. These plates are commonly used in clinical laboratories to isolate and identify Streptococcus species, which are known to cause various infections in humans. BAP is a general-purpose medium that supports the growth of most bacteria, while CNA is selective for Gram-positive bacteria, including Streptococcus. SXT agar is used to detect the presence of Group B Streptococcus in pregnant women.Rate this question:
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- 3.
A stool cultures usually uses which plates?
- A.
BAP, CHOC, CNA, MAC
- B.
BAP, CNA, MAC, MAC w/SORB
- C.
BAP, CNA, MAC, MAC w/SORB, HE
- D.
BAP, CNA, MAC, MAC w/SORB, HE, CHOC
Correct Answer
C. BAP, CNA, MAC, MAC w/SORB, HE -
- 4.
IMViC is the pneumonic for
- A.
Indole, Methyl Red, VP, Citrate
- B.
Indole, Methyl Red, VP, Catalase
- C.
Inducible, Methyl Red, VP, Citrate
- D.
Indole, Methyl Red, VP, Coagulase
Correct Answer
A. Indole, Methyl Red, VP, CitrateExplanation
IMViC is a mnemonic used to remember a series of biochemical tests commonly used in microbiology to identify enteric bacteria. The correct answer, "Indole, Methyl Red, VP, Citrate", represents the four tests included in the IMViC series. Indole test is used to detect the production of indole from the breakdown of tryptophan, Methyl Red test is used to determine the ability of an organism to perform mixed acid fermentation, Voges-Proskauer (VP) test is used to detect the production of acetoin, and Citrate test is used to determine the ability of an organism to utilize citrate as a sole carbon source.Rate this question:
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- 5.
What organism is IMViC ++--
- A.
E. Coli
- B.
PsA
- C.
Staph Aureus
- D.
Citrobactor
Correct Answer
A. E. ColiExplanation
The IMViC test is a series of four biochemical tests used to identify bacteria in the Enterobacteriaceae family. The letters in IMViC stand for the tests: Indole, Methyl Red, Voges-Proskauer, and Citrate. The result "++--" indicates that the organism is positive for the Indole test, negative for the Methyl Red test, negative for the Voges-Proskauer test, and negative for the Citrate test. Among the given options, E. Coli is the only organism known to produce these specific results in the IMViC test.Rate this question:
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- 6.
What organism is IMViC - + - -
- A.
E. Coli
- B.
PsA
- C.
Shigella
- D.
Citrobactor
Correct Answer
C. ShigellaExplanation
The IMViC test is a series of four biochemical tests used to differentiate between different species of bacteria. In this case, the result of IMViC - + - - suggests that the organism is Shigella. Each letter in IMViC represents a different test: I for indole production (negative), M for methyl red test (positive), V for Voges-Proskauer test (negative), and C for citrate utilization test (negative). Shigella is known to exhibit this specific combination of test results, leading to the conclusion that it is the correct answer.Rate this question:
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- 7.
What organism is IMViC - + - +
- A.
E. Coli
- B.
PsA
- C.
Shigella
- D.
Salmonella
Correct Answer
D. SalmonellaExplanation
The IMViC test is a series of biochemical tests used to identify certain enteric bacteria. The pattern IMViC - + - + indicates that the organism is negative for indole production, positive for methyl red test, negative for Voges-Proskauer test, and positive for citrate utilization. Among the given options, Salmonella is the only organism that fits this pattern. E. Coli is positive for indole production, PsA is positive for Voges-Proskauer test, and Shigella is negative for both methyl red test and citrate utilization.Rate this question:
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- 8.
What organism is IMViC - - + +
- A.
E. Coli
- B.
Klebsiella
- C.
Shigella
- D.
Salmonella
Correct Answer
B. KlebsiellaExplanation
The IMViC test is used to differentiate between different species of bacteria based on their metabolic characteristics. The + - - + pattern indicates that the organism is negative for indole production, positive for methyl red test, negative for Voges-Proskauer test, and positive for citrate utilization. Among the given options, Klebsiella is the only organism that matches this pattern. Therefore, the correct answer is Klebsiella.Rate this question:
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- 9.
What organism is IMViC ++--
- A.
E. Coli
- B.
Proteus Vulgaris
- C.
Staph Aureus
- D.
Proteus Mirabilis
Correct Answer
B. Proteus VulgarisExplanation
The IMViC test is a series of tests used to differentiate between different species of bacteria. In this case, the answer "Proteus Vulgaris" is correct because it is the only organism listed that matches the IMViC pattern of ++--. E. Coli, Staph Aureus, and Proteus Mirabilis do not match this pattern.Rate this question:
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- 10.
What organism is IMViC - + - +
- A.
E. Coli
- B.
Proteus Vulgaris
- C.
Staph Aureus
- D.
Proteus Mirabilis
Correct Answer
D. Proteus MirabilisExplanation
The IMViC test is a series of four tests used to identify certain bacteria, including Escherichia coli (E. coli), Proteus Vulgaris, Staphylococcus aureus (Staph Aureus), and Proteus Mirabilis. The results of the IMViC test for the given organism show a positive result for the Indole test (I), a negative result for the Methyl Red test (M), a positive result for the Voges-Proskauer test (V), and a negative result for the Citrate test (C). Based on these results, the correct answer is Proteus Mirabilis.Rate this question:
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- 11.
What organism is IMViC - + - +
- A.
E. Coli
- B.
Proteus Vulgaris
- C.
Staph Aureus
- D.
Citrobactor
Correct Answer
D. CitrobactorExplanation
The IMViC test is used to identify certain Enterobacteriaceae, a family of bacteria that includes Citrobacter. The test involves four different reactions: Indole production (I), Methyl Red test (M), Voges-Proskauer test (V), and Citrate utilization (C). The given result "IMViC - + - +" suggests that the organism is negative for indole production (I-), positive for methyl red test (M+), negative for Voges-Proskauer test (V-), and positive for citrate utilization (C+). Among the options provided, Citrobacter is the only organism that matches this pattern.Rate this question:
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- 12.
What organism is IMViC - - + +
- A.
E. Coli
- B.
Enterobacter
- C.
Shigella
- D.
Salmonella
Correct Answer
B. Enterobacter -
- 13.
What does TSI agar mean?
- A.
Triphosphate iron
- B.
Triple sugar iron
- C.
Triple sucrose iron
- D.
Ten sugar ion
Correct Answer
B. Triple sugar ironExplanation
TSI agar refers to triple sugar iron agar, which is a type of agar medium used in microbiology to differentiate and identify bacteria based on their ability to ferment sugars and produce hydrogen sulfide gas. The medium contains three sugars (glucose, lactose, and sucrose) and iron salts. Bacteria that can ferment any of these sugars produce acid, which causes a color change in the agar. Additionally, bacteria that can produce hydrogen sulfide gas will react with the iron salts, resulting in the formation of a black precipitate. Therefore, the correct answer is "triple sugar iron."Rate this question:
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- 14.
What does TSI contain?
- A.
Phenol red, glucose, lactose, sucrose, iron
- B.
Glucose, lactose, sucrose, ions
- C.
Methyl red, glucose, lactose, sucrose
- D.
VP, glucose, lactose, sucrose, iron
- E.
A
Correct Answer
A. Phenol red, glucose, lactose, sucrose, ironExplanation
TSI (Triple Sugar Iron) is a type of agar medium used in microbiology to differentiate bacteria based on their ability to ferment different sugars and produce hydrogen sulfide gas. The correct answer, "phenol red, glucose, lactose, sucrose, iron," refers to the components present in TSI. Phenol red is the pH indicator that turns yellow in the presence of acid produced during sugar fermentation. Glucose, lactose, and sucrose are the three sugars present in TSI, which bacteria can ferment. Iron is added to detect the production of hydrogen sulfide gas, which reacts with iron to form a black precipitate.Rate this question:
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- 15.
What does this TSI Indicate
- A.
K/A-
- B.
K/A+
- C.
A/A-
- D.
A/A+
Correct Answer
A. K/A-Explanation
The TSI indicates the presence of antibodies in the blood that are specific to the bacteria that causes Klebsiella pneumoniae. The "K" stands for Klebsiella and the "A-" indicates the presence of antibodies against it. This result suggests that the person has been exposed to or has an ongoing infection with Klebsiella pneumoniae.Rate this question:
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- 16.
What does this TSI Indicate
- A.
A/A H2S negative with gas
- B.
K/A-
- C.
K/A+
- D.
A/A-
Correct Answer
C. K/A+Explanation
The TSI (Triple Sugar Iron) test is used to differentiate bacteria based on their ability to ferment sugars and produce gas. The given answer "K/A+" indicates that the organism is capable of fermenting glucose (K) but not lactose or sucrose (A), and it produces gas (+). This suggests that the organism is a glucose fermenter and gas producer, but does not ferment lactose or sucrose.Rate this question:
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- 17.
What does this TSI Indicate
- A.
K/A-
- B.
K/A+
- C.
A/A-
- D.
A/A+
Correct Answer
D. A/A+Explanation
The TSI (Thyroid Stimulating Immunoglobulin) measures the presence of antibodies in the blood that stimulate the thyroid gland. In this case, an A/A+ result indicates a high level of thyroid-stimulating antibodies, suggesting that the individual may have Graves' disease, an autoimmune disorder that causes the thyroid gland to produce too much thyroid hormone.Rate this question:
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- 18.
What does this TSI Indicate
- A.
K/A-
- B.
K/A+
- C.
A/A-
- D.
A/A+
Correct Answer
C. A/A-Explanation
This TSI (Thyroid Stimulating Immunoglobulin) indicates that the patient has high levels of thyroid-stimulating antibodies, which can lead to an overactive thyroid gland. The "A/A-" indicates the presence of these antibodies, suggesting a potential diagnosis of Graves' disease, an autoimmune disorder that causes hyperthyroidism. This condition can result in symptoms such as weight loss, rapid heartbeat, and anxiety. Treatment options may include medication to suppress thyroid function or surgery to remove the thyroid gland.Rate this question:
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- 19.
What does this TSI Indicate
- A.
K/A-
- B.
K/A+
- C.
A/A-
- D.
A/A H2S negative with gas
Correct Answer
D. A/A H2S negative with gasExplanation
The TSI (Triple Sugar Iron) test is used to determine the ability of an organism to ferment sugars and produce gas, as well as its ability to produce hydrogen sulfide (H2S). In this case, the TSI indicates that the organism is able to ferment both glucose and lactose (A/A), does not produce hydrogen sulfide (H2S negative), and produces gas.Rate this question:
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- 20.
What common organism(s) might this TSI indicate?
- A.
E. Coli,Enterobactor,Klebsiella
- B.
Shigella, Citrobacter,Providencia,Proteus,Salmonella,Yersinia
- C.
Salmonella, Citrobacter, or Edwardsiella
- D.
Serratia,E. Coli,Enterobacter,Klebsiella
Correct Answer
B. Shigella, Citrobacter,Providencia,Proteus,Salmonella,YersiniaExplanation
The correct answer indicates that the TSI might indicate the presence of Shigella, Citrobacter, Providencia, Proteus, Salmonella, and Yersinia.Rate this question:
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- 21.
What common organism(s) might this TSI indicate?
- A.
E. Coli,Enterobactor,Klebsiella
- B.
Shigella, Citorbacter,Providencia,Proteus,Salmonella,Yersinia
- C.
Salmonella, Citrobacter, or Edwardsiella
- D.
Serratia,E. Coli,Enterobacter,Klebsiella
Correct Answer
C. Salmonella, Citrobacter, or EdwardsiellaExplanation
This TSI indicates the presence of Salmonella, Citrobacter, or Edwardsiella.Rate this question:
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- 22.
What common organism(s) might this TSI indicate?
- A.
E. Coli,Enterobactor,Klebsiella
- B.
Shigella, Citorbacter,Providencia,Proteus,Salmonella,Yersinia
- C.
Salmonella, Citrobacter, or Edwardsiella
- D.
Serratia,E. Coli,Enterobacter,Klebsiella
Correct Answer
D. Serratia,E. Coli,Enterobacter,KlebsiellaExplanation
This TSI indicates the presence of Serratia, E. Coli, Enterobacter, and Klebsiella.Rate this question:
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- 23.
What common organism(s) might this TSI indicate?
- A.
Citrobacter,Salmonella, Arizonae,Proteus
- B.
Shigella, Citorbacter,Providencia,Proteus,Salmonella,Yersinia
- C.
Salmonella, Citrobacter, or Edwardsiella
- D.
Serratia,E. Coli,Enterobacter,Klebsiella
Correct Answer
A. Citrobacter,Salmonella, Arizonae,ProteusExplanation
The TSI (Triple Sugar Iron) test is used to differentiate between different species of Enterobacteriaceae based on their ability to ferment sugars and produce gas. The given correct answer includes Citrobacter, Salmonella, Arizonae, and Proteus, which are all common organisms that can be indicated by the TSI test. These organisms are known to ferment sugars and produce gas, which is characteristic of a positive TSI reaction.Rate this question:
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- 24.
What common organism(s) might this TSI indicate?
- A.
E. Coli,Enterobactor,Klebsiella
- B.
Shigella, Citorbacter,Providencia,Proteus,Salmonella,Yersinia
- C.
Salmonella, Citrobacter, or Edwardsiella
- D.
Serratia,E. Coli,Enterobacter,Klebsiella
Correct Answer
A. E. Coli,Enterobactor,KlebsiellaExplanation
This TSI indicates the presence of E. Coli, Enterobactor, and Klebsiella.Rate this question:
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- 25.
True or False This is GPC CL
- A.
True
- B.
False
Correct Answer
B. False -
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Sep 04, 2023Quiz Edited by
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Mar 05, 2015Quiz Created by
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