Trivia Questions Quiz On Amnis Imaging Cytometer!
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Trivia Questions Quiz On Amnis Imaging Cytometer! The human body is made up of different cells, and through imaging cytometer, you can observe the cells on a microscopic level and characterize hematological malignancies. Do you know the different things you can see through this method? Take this quiz and get to learn more about the imagining cytometer. All the best!
- 1.Traditional flow cytometry is useful for:
- A.
Discriminating cells based on changes in intensity
- B.
Determining shape changes
- C.
Determining co-localization events
- D.
Investigating cellular trafficking and polarization
- E.
Discriminating cells based on their appearance
- 2.Traditional fluorescence microscopy is limited by the following problems:
- A.
Difficult to acquire and analyze a statistically large number of events per sample
- B.
Images are laborious and time consuming to acquire
- C.
Poor resolution for blood cells
- D.
Difficult to quantify images in a standardized and objective manner despite the tremendous amount of information present in each image
- 3.Combining flow cytometry and microscopy in a single platform enables:
- A.
Visual feedback for no guesswork gating
- B.
Discrimination of debris and other artifacts from real cells
- C.
Identification of cell conjugates or doublets
- D.
Identification of surface or intracellular staining
- E.
Quantitative image analysis of rare cells
- F.
Physical sorting of cells based on morphology for use in downstream experiments
- G.
Objective collection and analysis of a statistically relevant number of images per sample
- H.
High speed image acquisition for statistical microscopy applications
- 4.Primary reason(s) for approaching Microscopists with Amnis technology:
- A.
Superior resolution
- B.
Pseudocolor representation of images
- C.
Statistical power ensures that image-based analysis is representative of the sample
- D.
The ability to stitch images together
- 5.Imaging in flow provides:
- A.
A means of verifying gates
- B.
The ability to measure the spatial distribution of signals
- C.
The ability to measure changes in appearance and intensity with statistical power
- D.
Z stack analysis for true confocal resolution
- 6.What is the advantage of having an image for immunophenotyping experiments?
- A.
Enables gating with confidence
- B.
Helps discrimate single cells expressing two surface markers from doublets
- C.
Verifies overall health of cells
- D.
Provides visual validation of staining protocol
- 7.
- A.
Yes, but it’s nice to have the pictures
- B.
Yes, so long as you use a live/dead nuclear dye
- C.
Yes, but only if you catch the correct time course
- D.
No, there is not really a discernible difference in FSC, SSC, or fluorescence intensity
- E.
No, SSC is too high to get an accurate read-out
- 8.
- A.
No, because you have to have tens of thousands of cells per sample
- B.
Yes, because you can reuse your sample
- C.
Yes, but results can be questionable due to subjective analysis of a small number of cells per sample, especially for rare events
- D.
No, western blot analysis is the only accepted method
- 9.
- A.
No, because you can't fractionate the cells into nuclear and cytoplasmic fractions
- B.
Yes, but you cannot get single cell translocation status or quantify translocation in subpopulations wihtin the sample
- C.
Yes, and it is the only method that works
- 10.Which kind of samples are well-suited for analysis using Amnis cytometers?
- A.
Whole blood leukocytes
- B.
Tissue sections
- C.
Mature plated neurons
- D.
Adherent cells not adaptable to flow
- E.
Adherent cells adaptable to flow
- F.
Non-adherent cell lines
- 11.Which of the following assay(s) are well-suited for analysis using Amnis cytometers?
- A.
Discrimination of double-positive single cells from conjugate artifacts
- B.
Quantification of chemokine-induced shape change in human monocytes
- C.
Quantification of nuclear translocation in rare, immunophenotypically-defined subsets within whole blood leukocyte preps
- D.
Evaluation of parasite burden within RBC
- E.
Quantification of cirrhosis-induced scarring on liver tissue sections
- 12.What are some advantages of conventional flow cytometry over conventional fluorescence microscopy?
- A.
Throughput is higher, allowing greater statistical power
- B.
More information per probe
- C.
Rare event analysis
- D.
Quantitative parameters include intensity, shape and co-localization metrics
- E.
Image visualization of dots on dot plots, enabling guess-free gating
- 13.What are some advantages of FlowSight Basic cytometry over conventional flow cytometry?
- A.
Throughput is higher, allowing greater statistical power
- B.
More information per probe
- C.
Rare event analysis
- D.
Quantitative parameters include intensity, shape and co-localization metrics
- E.
Image visualization, enabling guess-free gating
- 14.What are some advantages of FlowSight Quantitative Imaging cytometry over conventional flow cytometry?
- A.
Throughput is higher, allowing greater statistical power
- B.
More information per probe
- C.
Rare event analysis
- D.
Quantitative parameters include intensity, shape and co-localization metrics
- E.
Image visualization, enabling guess-free gating
- 15.FlowSight:
- A.
Is designed for traditional flow applications
- B.
Provides imagery in up to 12 channels of every acquired cell
- C.
Acquires brightfield, darkfield, and fluorescence images
- D.
Can measure size, SSC and Intensity, plus additional morphology-based parameters
- E.
Has a 785 nm Laser dedicated to Side Scatter and optimized for WBCs
- F.
Is an excellent protocol development tool
- G.
Requires collecting a minimum of 10,000 images
- H.
Can generate FCS-compatible data
- 16.FlowSight Quantitative Imaging (QI) upgrade includes:
- A.
10 Channel brightfield for flexibility in flow panel design
- B.
An autosampler
- C.
Enhanced image analysis features with IDEAS
- D.
Enhanced 488 laser power for high sensitivity imaging
- E.
Wizard-based analysis for ease of use
- 17.Autosampler: which of the following is correct?
- A.
At lowest resolution, can complete ten 384-well plates in one day, and is therefore well suited for primary screening labs
- B.
Can run multiple experiments on the same 96-well plate
- C.
Can be configured to send email notification for well failures
- D.
Can be run overnight
- 18.
![Which system would better discriminate dim signals from background?]( "Which system would better discriminate dim signals from background?")
Which system would better discriminate dim signals from background?- A.
A (on left)
- B.
B (on right)
- C.
Both are similar
- 19.The Experiment Evaluation Worksheet:
- A.
Directs researcher toward applications that leverage unique benefits of the platform
- B.
Clarifies goals of the sample evaluation
- C.
Provides specific details for planning the evaluation samples
- D.
Is required for exporting images
- E.
Is used to design a practical experiment that efficiently evaluates the platform’s unique value
- F.
Begins documentation of the evaluation process
- 20.Dot plots and histograms in IDEAS:
- A.
Cannot be used to recapitulate plots from conventional flow cytometers
- B.
Are also used in standard flow cytometry
- C.
Can be used to plot many types of parameters in addition to fluorescence intensity
- D.
Are linked directly to the images they represent, enabling visual verification of dots and histogram bins
- 21.Which of the following statement(s) regarding the ImageStream and FlowSight are true?
- A.
Both provide 40X imagery
- B.
All quantitave data for both platforms are derived from images
- C.
The Quantitative Imaging (QI) capability comes standard on the ISX, but is an option for FlowSight
- D.
Both can be upgraded with the Extended Depth of Field (EDF) element
- E.
Both enable image visualization and therefore guess-free gating
- 22.Consider the ImageStream endosomal and lysosomal co-localization experiment described in the Jan 5 Introductory webinar. Which of the following statement(s) are true?
- A.
ImageStream provides objective collection of images, thereby eliminating operator collection bias that can occur with manual image acquisition on a traditional fluorescence microscope
- B.
ImageStream high acquisition rate provides a statistically significant number of events to analyze, allowing the researcher to conclude that there is a time-dependent accumulation of CpGB in the lysosome
- C.
ImageStream provides superior image resolution compared to confocal microscopy, and for this reason is the instrument of choice for this assay
- D.
ImageStream does not have the optical resolution to measure co-localization, and for this reason should not be used for this assay
- 23.What are the primary value propositions of Amnis technology?
- A.
The value that imagery brings to flow cytometry
- B.
The value that population statistics brings to microscopy
- C.
Visual feedback for no guesswork gating
- D.
The power of combining flow cytometry with microscopy as a protocol development tool
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