Trivia Questions Quiz On Amnis Imaging Cytometer! The human body is made up of different cells, and through imaging cytometer, you can observe the cells on a microscopic level and characterize hematological malignancies. Do you know the different things you can see through this method? Take this quiz and get to learn more about the imagining cytometer. All the best!
No, because you have to have tens of thousands of cells per sample
Yes, because you can reuse your sample
Yes, but results can be questionable due to subjective analysis of a small number of cells per sample, especially for rare events
No, western blot analysis is the only accepted method
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Yes, but it’s nice to have the pictures
Yes, so long as you use a live/dead nuclear dye
Yes, but only if you catch the correct time course
No, there is not really a discernible difference in FSC, SSC, or fluorescence intensity
No, SSC is too high to get an accurate read-out
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Superior resolution
Pseudocolor representation of images
Statistical power ensures that image-based analysis is representative of the sample
The ability to stitch images together
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No, because you can't fractionate the cells into nuclear and cytoplasmic fractions
Yes, but you cannot get single cell translocation status or quantify translocation in subpopulations wihtin the sample
Yes, and it is the only method that works
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Whole blood leukocytes
Tissue sections
Mature plated neurons
Adherent cells not adaptable to flow
Adherent cells adaptable to flow
Non-adherent cell lines
ImageStream provides objective collection of images, thereby eliminating operator collection bias that can occur with manual image acquisition on a traditional fluorescence microscope
ImageStream high acquisition rate provides a statistically significant number of events to analyze, allowing the researcher to conclude that there is a time-dependent accumulation of CpGB in the lysosome
ImageStream provides superior image resolution compared to confocal microscopy, and for this reason is the instrument of choice for this assay
ImageStream does not have the optical resolution to measure co-localization, and for this reason should not be used for this assay
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Discriminating cells based on changes in intensity
Determining shape changes
Determining co-localization events
Investigating cellular trafficking and polarization
Discriminating cells based on their appearance
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The value that imagery brings to flow cytometry
The value that population statistics brings to microscopy
Visual feedback for no guesswork gating
The power of combining flow cytometry with microscopy as a protocol development tool
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Visual feedback for no guesswork gating
Discrimination of debris and other artifacts from real cells
Identification of cell conjugates or doublets
Identification of surface or intracellular staining
Quantitative image analysis of rare cells
Physical sorting of cells based on morphology for use in downstream experiments
Objective collection and analysis of a statistically relevant number of images per sample
High speed image acquisition for statistical microscopy applications
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A means of verifying gates
The ability to measure the spatial distribution of signals
The ability to measure changes in appearance and intensity with statistical power
Z stack analysis for true confocal resolution
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Discrimination of double-positive single cells from conjugate artifacts
Quantification of chemokine-induced shape change in human monocytes
Quantification of nuclear translocation in rare, immunophenotypically-defined subsets within whole blood leukocyte preps
Evaluation of parasite burden within RBC
Quantification of cirrhosis-induced scarring on liver tissue sections
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Cannot be used to recapitulate plots from conventional flow cytometers
Are also used in standard flow cytometry
Can be used to plot many types of parameters in addition to fluorescence intensity
Are linked directly to the images they represent, enabling visual verification of dots and histogram bins
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Difficult to acquire and analyze a statistically large number of events per sample
Images are laborious and time consuming to acquire
Poor resolution for blood cells
Difficult to quantify images in a standardized and objective manner despite the tremendous amount of information present in each image
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Both provide 40X imagery
All quantitave data for both platforms are derived from images
The Quantitative Imaging (QI) capability comes standard on the ISX, but is an option for FlowSight
Both can be upgraded with the Extended Depth of Field (EDF) element
Both enable image visualization and therefore guess-free gating
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Throughput is higher, allowing greater statistical power
More information per probe
Rare event analysis
Quantitative parameters include intensity, shape and co-localization metrics
Image visualization of dots on dot plots, enabling guess-free gating
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Enables gating with confidence
Helps discrimate single cells expressing two surface markers from doublets
Verifies overall health of cells
Provides visual validation of staining protocol
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At lowest resolution, can complete ten 384-well plates in one day, and is therefore well suited for primary screening labs
Can run multiple experiments on the same 96-well plate
Can be configured to send email notification for well failures
Can be run overnight
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Directs researcher toward applications that leverage unique benefits of the platform
Clarifies goals of the sample evaluation
Provides specific details for planning the evaluation samples
Is required for exporting images
Is used to design a practical experiment that efficiently evaluates the platform’s unique value
Begins documentation of the evaluation process
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Is designed for traditional flow applications
Provides imagery in up to 12 channels of every acquired cell
Acquires brightfield, darkfield, and fluorescence images
Can measure size, SSC and Intensity, plus additional morphology-based parameters
Has a 785 nm Laser dedicated to Side Scatter and optimized for WBCs
Is an excellent protocol development tool
Requires collecting a minimum of 10,000 images
Can generate FCS-compatible data
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Throughput is higher, allowing greater statistical power
More information per probe
Rare event analysis
Quantitative parameters include intensity, shape and co-localization metrics
Image visualization, enabling guess-free gating
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Throughput is higher, allowing greater statistical power
More information per probe
Rare event analysis
Quantitative parameters include intensity, shape and co-localization metrics
Image visualization, enabling guess-free gating
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10 Channel brightfield for flexibility in flow panel design
An autosampler
Enhanced image analysis features with IDEAS
Enhanced 488 laser power for high sensitivity imaging
Wizard-based analysis for ease of use
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