Microbiology MCQ Trivia Questions!
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The study of microbiology deals with the many different organisms that we are typically unable to see with the naked eye due to how small they are – i. E. They can only truly be identified and analyzed with a microscope. In the following quiz, we’re going to test your knowledge on these organisms by offering you a multiple-choice quiz on the topic. Think you know your stuff? Let’s find out.
- 1.
Which of the following media is used for culturing Salmonella?
- A.
VL-broth
- B.
Sabouraud agar
- C.
Slanetz Bartley
- D.
Selenite Broth
Correct Answer
D. Selenite BrothExplanation
VL-broth is used for anaerobes. Sabouraud agar is used for fungi. Slanetz-Bartley is used for enterococci. Selenite broth is an example of a Selectively Multiplying liquid media used to culture Salmonella.Rate this question:
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- 2.
Arabinose is a test-tube test used for:
- A.
Enterococci
- B.
Salmonella
- C.
G- bacteria
- D.
Neisseriae
Correct Answer
A. EnterococciExplanation
Turns yellow = positive, remains green = negative.Rate this question:
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- 3.
Choose the incorrect statement about Endo agar:
- A.
Only some G+ bacteria can grow on it selectively
- B.
Can be used to differentiate lactose fermentation
- C.
Is similar to McConkey medium
- D.
Combines selective and diagnostic properties
Correct Answer
A. Only some G+ bacteria can grow on it selectivelyExplanation
Only some G- bacteria can grow on it selectively.Rate this question:
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- 4.
Choose the correct statement about Antigen detection:
- A.
It is an indirect method
- B.
Negative result means presence of microbe in the patient’s body
- C.
It is carried out in the laboratory using antibodies of animal origin
- D.
Uses a sample of patients saliva
Correct Answer
C. It is carried out in the laboratory using antibodies of animal originExplanation
Iit is a direct method. Positive result means presence of the microbe in the patient‘s body.Rate this question:
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- 5.
The third phase of the PCR reaction involves:
- A.
Detection of amplification product
- B.
Amplification of DNA
- C.
Breakdown of DNA
- D.
Obtaining isolated DNA
Correct Answer
A. Detection of amplification productExplanation
The amplification product should be detected by gel electrophoresis or ELISA in third phase.Rate this question:
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- 6.
Chocolat agar is an example of a pure enriched medium used for culturing:
- A.
Streptococci
- B.
Hemophili
- C.
Enterococci
- D.
Fungi
Correct Answer
B. HemophiliExplanation
It is used for pathogenous Neisseriae and Hemophili (that do not grow even on blood agar)Rate this question:
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- 7.
When using Hanja medium, if the colour of the whole medium is yellow it means:
- A.
Formation of H2S
- B.
Bacterium is not a glucose fermenter
- C.
Bacterium is a lactose fermenter
- D.
Bacterium is not a lactose fermenter
Correct Answer
C. Bacterium is a lactose fermenterExplanation
Formation of H2S causes bottom part of medium to turn black.Rate this question:
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- 8.
Optochin is used to test:
- A.
Lactose fermentation
- B.
Motility
- C.
Glucose fermentation
- D.
Antibiotic susceptibility
Correct Answer
D. Antibiotic susceptibilityExplanation
for answers b) c) and d), Hanja medium + MIU is used.Rate this question:
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- 9.
Löwentein-Jensen medium is used for culturing:
- A.
Enterobacteria
- B.
Tuberculosis
- C.
Salmonella
- D.
G+ bacteria
Correct Answer
B. TuberculosisExplanation
It is a green coloured solid enriched media in a test tube, used for TBC.Rate this question:
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- 10.
Arabinose is a test-tube test used for:
- A.
Salmonella
- B.
Neisseriae
- C.
Enterococci
- D.
G- bacteria
Correct Answer
C. EnterococciExplanation
Turns yellow = positive, remains green = negative.Rate this question:
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- 11.
Choose the incorrect statement about E-tests
- A.
The testing strip has a rising Atb concentration
- B.
The test is qualitative
- C.
The strip has a scale
- D.
Principally similar to diffusion disc test
Correct Answer
B. The test is qualitativeExplanation
The E-test is quantitativeRate this question:
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- 12.
The MIC Value is:
- A.
The highest concentration that inhibits growth
- B.
The highest concentration that allows growth
- C.
The lowest concentration that inhibits growth
- D.
The lowest concentration that inhibits growth
Correct Answer
D. The lowest concentration that inhibits growthExplanation
MIC value is used in the microdilution test.Rate this question:
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- 13.
In microdilution testing, if MIC Value > breakpoint;
- A.
Strain is suceptible
- B.
Strain is resistant
- C.
A different test is needed
- D.
None of the answers are correct
Correct Answer
B. Strain is resistantExplanation
MIC > breakpoint
= strain is resistantRate this question:
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- 14.
Haemagluttination Inhibition Test:
- A.
Is not an agglutination reaction
- B.
Is a neutralisation reaction
- C.
Result with a dense round target is positive
- D.
All answers are correct
Correct Answer
D. All answers are correctExplanation
Example of use: we can read HIT results for tick-born encephalitis.Rate this question:
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- 15.
Choose the correct statement about ASO:
- A.
Is a direct diagnostics reaction
- B.
The aim is to find a pathogen
- C.
ASO examination is indicated in rheumatoid fever
- D.
All of the answers are correct
Correct Answer
C. ASO examination is indicated in rheumatoid feverExplanation
ASO examination is indicated in rheumatoid fever. Rheumatoid fever is a condition that can occur after a streptococcal infection, and it is characterized by inflammation in the joints, heart, and other organs. ASO (Anti-Streptolysin O) is a test that measures the level of antibodies in the blood against the streptococcal bacteria. An elevated level of ASO antibodies indicates a recent streptococcal infection, which can be a potential cause of rheumatoid fever. Therefore, performing an ASO examination is recommended in cases of suspected rheumatoid fever to confirm the presence of a streptococcal infection.Rate this question:
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- 16.
Which of the following is NOT considered a classification of PCR:
- A.
Specific PCR
- B.
Multiplex PCR
- C.
Universal PCR
- D.
Segmental PCR
Correct Answer
D. Segmental PCRExplanation
Segmental PCR is not considered a classification of PCR because it is not a commonly recognized or widely used term in the field of PCR. Specific PCR, Multiplex PCR, and Universal PCR are all well-established classifications of PCR techniques. Specific PCR refers to the amplification of a specific target sequence, Multiplex PCR involves the simultaneous amplification of multiple target sequences in a single reaction, and Universal PCR is a technique used to amplify a wide range of target sequences.Rate this question:
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- 17.
In the first phase of PCR reaction:
- A.
We have to find isolated DNA
- B.
Product is initially detected
- C.
We have to amplify the DNA
- D.
None of the answers are correct
Correct Answer
A. We have to find isolated DNAExplanation
In the first phase of PCR reaction, the main objective is to find isolated DNA. This implies that the DNA needs to be separated and purified from other cellular components before proceeding with the PCR reaction. The isolation of DNA is a crucial step to ensure accurate and reliable results in PCR.Rate this question:
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- 18.
Catalase test is used for:
- A.
Diagnostics of G+ cocci
- B.
Diagnostics of G+ rods
- C.
Uses hydrogen peroxide
- D.
All answers are correct
Correct Answer
D. All answers are correctExplanation
The catalase test is used for the diagnostics of both Gram-positive cocci and Gram-positive rods. It involves the use of hydrogen peroxide to determine the presence of the enzyme catalase, which breaks down hydrogen peroxide into water and oxygen. Since all the given answers correctly describe the purpose and use of the catalase test, the answer "all answers are correct" is appropriate.Rate this question:
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- 19.
Indol test positivity is indicated by presence of:
- A.
A red ring
- B.
Colour change to yellow
- C.
Colour change to blue
- D.
Increased turbidity
Correct Answer
A. A red ringExplanation
Is used as part of MIU complex test tube test.Rate this question:
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- 20.
Octal codes are used in:
- A.
MIU test
- B.
Optochin test
- C.
Plate tests
- D.
Catalase test
Correct Answer
C. Plate testsExplanation
It is a mathematical binary system used in the plate tests., e.g. ENTEROtest16Rate this question:
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Our quizzes are rigorously reviewed, monitored and continuously updated by our expert board to maintain accuracy, relevance, and timeliness.
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Current Version
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Nov 16, 2023Quiz Edited by
ProProfs Editorial Team -
Nov 16, 2011Quiz Created by
Bosscz
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