Microbiology MCQ Trivia Questions!

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Microbiology MCQ Trivia Questions! - Quiz

The study of microbiology deals with the many different organisms that we are typically unable to see with the naked eye due to how small they are – i. E. They can only truly be identified and analyzed with a microscope. In the following quiz, we’re going to test your knowledge on these organisms by offering you a multiple-choice quiz on the topic. Think you know your stuff? Let’s find out.


Questions and Answers
  • 1. 

    Which of the following media is used for culturing Salmonella?

    • A.

      VL-broth

    • B.

      Sabouraud agar

    • C.

      Slanetz Bartley

    • D.

      Selenite Broth

    Correct Answer
    D. Selenite Broth
    Explanation
    VL-broth is used for anaerobes. Sabouraud agar is used for fungi. Slanetz-Bartley is used for enterococci. Selenite broth is an example of a Selectively Multiplying liquid media used to culture Salmonella.

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  • 2. 

    Arabinose is a test-tube test used for:

    • A.

      Enterococci

    • B.

      Salmonella

    • C.

      G- bacteria

    • D.

      Neisseriae

    Correct Answer
    A. Enterococci
    Explanation
    Turns yellow = positive, remains green = negative.

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  • 3. 

    Choose the incorrect statement about Endo agar:

    • A.

      Only some G+ bacteria can grow on it selectively

    • B.

      Can be used to differentiate lactose fermentation

    • C.

      Is similar to McConkey medium

    • D.

      Combines selective and diagnostic properties

    Correct Answer
    A. Only some G+ bacteria can grow on it selectively
    Explanation
    Only some G- bacteria can grow on it selectively.

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  • 4. 

    Choose the correct statement about Antigen detection:

    • A.

      It is an indirect method

    • B.

      Negative result means presence of microbe in the patient’s body

    • C.

      It is carried out in the laboratory using antibodies of animal origin

    • D.

      Uses a sample of patients saliva

    Correct Answer
    C. It is carried out in the laboratory using antibodies of animal origin
    Explanation
    Iit is a direct method. Positive result means presence of the microbe in the patient‘s body.

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  • 5. 

    The third phase of the PCR reaction involves:

    • A.

      Detection of amplification product

    • B.

      Amplification of DNA

    • C.

      Breakdown of DNA

    • D.

      Obtaining isolated DNA

    Correct Answer
    A. Detection of amplification product
    Explanation
    The amplification product should be detected by gel electrophoresis or ELISA in third phase.

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  • 6. 

    Chocolat agar is an example of a pure enriched medium used for culturing:

    • A.

      Streptococci

    • B.

      Hemophili

    • C.

      Enterococci

    • D.

      Fungi

    Correct Answer
    B. Hemophili
    Explanation
    It is used for pathogenous Neisseriae and Hemophili (that do not grow even on blood agar)

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  • 7. 

    When using Hanja medium, if the colour of the whole medium is yellow it means:

    • A.

      Formation of H2S

    • B.

      Bacterium is not a glucose fermenter

    • C.

      Bacterium is a lactose fermenter

    • D.

      Bacterium is not a lactose fermenter

    Correct Answer
    C. Bacterium is a lactose fermenter
    Explanation
    Formation of H2S causes bottom part of medium to turn black.

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  • 8. 

    Optochin is used to test:

    • A.

      Lactose fermentation

    • B.

      Motility

    • C.

      Glucose fermentation

    • D.

      Antibiotic susceptibility

    Correct Answer
    D. Antibiotic susceptibility
    Explanation
    for answers b) c) and d), Hanja medium + MIU is used.

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  • 9. 

    Löwentein-Jensen medium is used for culturing:

    • A.

      Enterobacteria

    • B.

      Tuberculosis

    • C.

      Salmonella

    • D.

      G+ bacteria

    Correct Answer
    B. Tuberculosis
    Explanation
    It is a green coloured solid enriched media in a test tube, used for TBC.

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  • 10. 

    Arabinose is a test-tube test used for:

    • A.

      Salmonella

    • B.

      Neisseriae

    • C.

      Enterococci

    • D.

      G- bacteria

    Correct Answer
    C. Enterococci
    Explanation
    Turns yellow = positive, remains green = negative.

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  • 11. 

    Choose the incorrect statement about E-tests

    • A.

      The testing strip has a rising Atb concentration

    • B.

      The test is qualitative

    • C.

      The strip has a scale

    • D.

      Principally similar to diffusion disc test

    Correct Answer
    B. The test is qualitative
    Explanation
    The E-test is quantitative

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  • 12. 

    The MIC Value is:

    • A.

      The highest concentration that inhibits growth

    • B.

      The highest concentration that allows growth

    • C.

      The lowest concentration that inhibits growth

    • D.

      The lowest concentration that inhibits growth

    Correct Answer
    D. The lowest concentration that inhibits growth
    Explanation
    MIC value is used in the microdilution test.

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  • 13. 

    In microdilution testing, if MIC Value > breakpoint;

    • A.

      Strain is suceptible

    • B.

      Strain is resistant

    • C.

      A different test is needed

    • D.

      None of the answers are correct

    Correct Answer
    B. Strain is resistant
    Explanation
    MIC > breakpoint

    = strain is resistant

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  • 14. 

    Haemagluttination Inhibition Test:

    • A.

      Is not an agglutination reaction

    • B.

      Is a neutralisation reaction

    • C.

      Result with a dense round target is positive

    • D.

      All answers are correct

    Correct Answer
    D. All answers are correct
    Explanation
    Example of use: we can read HIT results for tick-born encephalitis.

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  • 15. 

    Choose the correct statement about ASO:

    • A.

      Is a direct diagnostics reaction

    • B.

      The aim is to find a pathogen

    • C.

      ASO examination is indicated in rheumatoid fever

    • D.

      All of the answers are correct

    Correct Answer
    C. ASO examination is indicated in rheumatoid fever
    Explanation
    ASO examination is indicated in rheumatoid fever. Rheumatoid fever is a condition that can occur after a streptococcal infection, and it is characterized by inflammation in the joints, heart, and other organs. ASO (Anti-Streptolysin O) is a test that measures the level of antibodies in the blood against the streptococcal bacteria. An elevated level of ASO antibodies indicates a recent streptococcal infection, which can be a potential cause of rheumatoid fever. Therefore, performing an ASO examination is recommended in cases of suspected rheumatoid fever to confirm the presence of a streptococcal infection.

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  • 16. 

    Which of the following is NOT considered a classification of PCR:

    • A.

      Specific PCR

    • B.

      Multiplex PCR

    • C.

      Universal PCR

    • D.

      Segmental PCR

    Correct Answer
    D. Segmental PCR
    Explanation
    Segmental PCR is not considered a classification of PCR because it is not a commonly recognized or widely used term in the field of PCR. Specific PCR, Multiplex PCR, and Universal PCR are all well-established classifications of PCR techniques. Specific PCR refers to the amplification of a specific target sequence, Multiplex PCR involves the simultaneous amplification of multiple target sequences in a single reaction, and Universal PCR is a technique used to amplify a wide range of target sequences.

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  • 17. 

    In the first phase of PCR reaction:

    • A.

      We have to find isolated DNA

    • B.

      Product is initially detected

    • C.

      We have to amplify the DNA

    • D.

      None of the answers are correct

    Correct Answer
    A. We have to find isolated DNA
    Explanation
    In the first phase of PCR reaction, the main objective is to find isolated DNA. This implies that the DNA needs to be separated and purified from other cellular components before proceeding with the PCR reaction. The isolation of DNA is a crucial step to ensure accurate and reliable results in PCR.

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  • 18. 

    Catalase test is used for:

    • A.

      Diagnostics of G+ cocci

    • B.

      Diagnostics of G+ rods

    • C.

      Uses hydrogen peroxide

    • D.

      All answers are correct

    Correct Answer
    D. All answers are correct
    Explanation
    The catalase test is used for the diagnostics of both Gram-positive cocci and Gram-positive rods. It involves the use of hydrogen peroxide to determine the presence of the enzyme catalase, which breaks down hydrogen peroxide into water and oxygen. Since all the given answers correctly describe the purpose and use of the catalase test, the answer "all answers are correct" is appropriate.

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  • 19. 

    Indol test positivity is indicated by presence of:

    • A.

      A red ring

    • B.

      Colour change to yellow

    • C.

      Colour change to blue

    • D.

      Increased turbidity

    Correct Answer
    A. A red ring
    Explanation
    Is used as part of MIU complex test tube test.

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  • 20. 

    Octal codes are used in:

    • A.

      MIU test

    • B.

      Optochin test

    • C.

      Plate tests

    • D.

      Catalase test

    Correct Answer
    C. Plate tests
    Explanation
    It is a mathematical binary system used in the plate tests., e.g. ENTEROtest16

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