Microbiology Final Exam
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The time has come, ladies and gentlemen, your efforts have led you to this moment and it’s time to see if they’ve gone to waste or not! We are, of course, talking about your microbiology final exam, in which we are going to test you on all of the knowledge you’ve recently acquired through your studies of microscopic organisms. Think you know all there is to know about them? Let’s find out!
- 1.Bacterial growth phase where cellular division seems to have stopped or ceases for a period of time:
- A.
The Lag Phase
- B.
The Log Phase
- C.
The Stationary Phase
- D.
The Death Phase
- 2.
- A.
Nutrient broth
- B.
Nutrient agar
- C.
Defined media
- D.
Complex media
- 3.Protection against osmotic lyses is provided by:
- A.
The cellular plasma membrane
- B.
The cell wall
- C.
The cell's capsule
- D.
Lysosomes
- 4.Thermophiles develop at temperatures between:
- A.
-5 and 20C
- B.
40 and 80C
- C.
-5 and 35C
- D.
65 and 105C
- 5.Name associate to the presence of a cell wall and a capsule together in a bacterial cell:
- A.
The cytoplasmic membrane
- B.
The Envelop
- C.
The Capsule
- D.
The Cell Wall
- 6.Which group do strict anaerobes partner with to find an environmental condition optimal for growth?
- A.
Obligated aerobes
- B.
Aerotolerant anaerobes
- C.
Microaerophiles
- D.
Facultative anaerobes
- 7.What statement is not descriptive of the Log Exponential Phase
- A.
Population growth is uniform
- B.
Growth rate is constant
- C.
Cells exhibit balanced growth
- D.
It varies in length
- 8.What is Microbiology?
- A.
The study of organisms too small to be seen with the naked eye.
- B.
Organism or agents too small to be visible with the naked eye.
- C.
Microorganisms relatively simple in their construction.
- D.
Microorganism that become nutrients to other organisms.
- 9.Organelle responsible for the transport/delivery of ER products across different parts of the cell.
- A.
The Golgi complex
- B.
The Smooth ER
- C.
The Rough ER
- D.
The Nucleus
- 10.Bacterial growth phase where a maximal rate of cellular division is obtained:
- A.
The Lag phase
- B.
The Log phase
- C.
The Stationary phase
- D.
The Death phase
- 11.Contributes to the negative charge of Gram - bacteria:
- A.
Broun's lipoproteins
- B.
Porins
- C.
Teichoic acids
- D.
LPS
- 12.A Pure Culture:
- A.
Presents a mixture of microbial organisms
- B.
Can only be obtained by isolating the progeny of single cell
- C.
Was first created by the efforts of microbiologist Luis Pasteur
- D.
Can be obtained by growing bacteria in differential media
- 13.
- A.
Bacteria that have lost all crystal violet staining after gram's alcohol washing
- B.
Bacteria that have retained crystal violet during staining
- C.
Bacteria that maintain their peptidoglycan layer
- D.
Bacteria that failed to counterstain with safranin
- 14.Organism that can only thrive at very specific concentrations of oxygen within a medium (2-10% oxygen):
- A.
Obligate aerobes
- B.
Facultative anaerobes
- C.
Aerotolerant anaerobes
- D.
Microaerophiles
- 15.Gram positive bacteria stain:
- A.
Purple
- B.
Orange
- C.
Black
- D.
Pink
- 16.Bacterial type that presents teichoic acids associated to it's peptidoglycan cell wall:
- A.
Gram +
- B.
Gram -
- C.
Archaea
- D.
Acids Fast
- 17.Which dye will be more likely to adhere to the surface of a gram positive bacteria?
- A.
Anionic
- B.
Cationic
- C.
Neutral
- D.
Differential
- 18.Present outside the plasma membrane f organisms of domain Archaea:
- A.
Slime Layer
- B.
Endospores
- C.
Glycocalyx
- D.
S-layer
- 19.What component of the LPS structure acts as an exotoxin:
- A.
The O antigen
- B.
The Lipid A region
- C.
The core polysaccharide
- D.
The thecoid acid
- 20.Which of the following is true about the lag phase:
- A.
Cells adapt to a new medium
- B.
In some cases it may be very short
- C.
Cells replenish spent materials
- D.
All of the above
- 21.The largest most diverse group of photosynthetic bacteria:
- A.
Yeast
- B.
Lichens
- C.
Cyanobacteria
- D.
Archaea
- 22.The first stage of prokaryotic cell division entails:
- A.
Cytokinesis
- B.
Growth of a septum that separates the mother cell into two separate chambers
- C.
Growth of the cell dividing it's chromosome and enlarging it's cell volume
- D.
None of the above
- 23.Process of movement of bacteria towards or away from a particular stimulus or toxic agent:
- A.
Chemotaxis
- B.
Pseudopodia
- C.
Cilia
- D.
None of the above
- 24.Lacks a peptidoglycan cell wall and lives in extreme environments such as the hot springs of the Yellowstone Park:
- A.
Bacteria
- B.
Yeast
- C.
Prokaryotes
- D.
Archaea
- 25.Organism too small to be seen with the naked eye:
- A.
Microbiology
- B.
Microorganisms
- C.
Insects
- D.
Monkey's
- 26.
- A.
Facultative anaerobe
- B.
Strict anaerobe
- C.
Obligate aerobe
- D.
None of the above
- 27.Term that describes when light neither passes through or bounces off an object or is transmitted through them:
- A.
Transmission
- B.
Absorption
- C.
Diffraction
- D.
Refraction
- 28.Temperature below that of optimal growth cause bacterial cultures to:
- A.
Perish quickly at high temperatures
- B.
Slow their growth rate as temperatures approach the minimum
- C.
Thrive without any evident damage to the cell
- D.
None of the above
- 29.Bacterial families whose optimal growth occurs between pH 5.5 and pH 7:
- A.
Alkalophiles
- B.
Neutrophiles
- C.
Acidophiles
- 30.Observed during the death phase, it presents successive waves of genetically distinct variants:
- A.
The Log phase
- B.
The Lag phase
- C.
Prolonged decline growth
- D.
The stationary phase
- 31.Staining procedure designed for bacterial families with high lipid content and resistant to standard staining:
- A.
Gram stain
- B.
Negative staining
- C.
Simple stain
- D.
Acid fast staining
- 32.Which of the following is not considered a starvation response?
- A.
Cell death at a logarithmic rate
- B.
A decrease in cellular size and nucleoid condensation
- C.
Synthesis of starvation proteins
- D.
Endospore formation
- 33.Organelle responsible for the synthesis of protein in a eukaryotic cell:
- A.
The Golgi complex
- B.
The Smooth ER
- C.
The rough ER
- D.
The nucleus
- 34.Bacterial families whose optimal growth occurs between pH 0 and 5.5:
- A.
Alkalophiles
- B.
Neutrophiles
- C.
Acidophiles
- 35.What statement is NOT a reason for entering the stationary phase:
- A.
Nutrient limitation
- B.
Limited oxygen availability
- C.
Unlimited sources of nutrients
- D.
Reaching a critical population density
- 36.
- A.
Microscopes use long wavelengths to resolve images better
- B.
The smaller the wavelength used the better the resolution
- C.
Resolution is the ability to see two objects as separate discrete units
- D.
If resolution is poor magnification is useless
- 37.Internal structure of a prokaryotic cell that form small bodies with accumulation of cellular material:
- A.
Endospores
- B.
The Nuclear region
- C.
The Lysosomes
- D.
Inclusion bodies
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