Introduction To Microbiology Quiz Questions And Answers
About This Quiz
The scientific study of biology deals with everything that we know about life on earth. Take this introduction to microbiology quiz questions and answers to check your knowledge. Biology has you covered, whether it’s the wonderful species in the animal kingdom, the plants that make up our environment, or even us ourselves. Today, however, we’ll be talking about those organisms See moreyou can’t see with the naked eye as we take a dive into microbiology! Let’s begin with this introductory quiz!
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- 2.
A portion of well-mixed urine can be aliquoted into B-D vacutainer urine C&S transport kit that contains freeze-dried preservative to preserve (prevent overgrowth of bacteria) during transport
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True
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False
Correct Answer
A. TrueExplanation
The statement is true because the B-D vacutainer urine C&S transport kit contains a freeze-dried preservative that helps preserve the urine and prevent the overgrowth of bacteria during transport. This allows for accurate testing of the urine sample without the risk of bacterial contamination.Rate this question:
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- 3.
A large sterile tube with a funnel-like insert to direct sputum into the tube is commonly used as a sputum collection system
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True
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False
Correct Answer
A. TrueExplanation
The statement is true because a large sterile tube with a funnel-like insert is indeed commonly used as a sputum collection system. This type of system helps to ensure that the sputum is collected in a clean and sterile manner, reducing the risk of contamination. The funnel-like insert helps to direct the sputum into the tube, making it easier to collect and transport for further analysis or testing. Overall, this type of collection system is widely used in healthcare settings for sputum collection.Rate this question:
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- 4.
Stool specimen in Cary Blair medium can be used for C&S.
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True
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False
Correct Answer
A. TrueExplanation
Cary Blair medium is a transport medium commonly used to preserve stool specimens for culture and sensitivity (C&S) testing. It contains a combination of ingredients that help maintain the viability of bacteria in the specimen during transportation to the laboratory. This allows for the isolation and identification of potential pathogens present in the stool, as well as testing their susceptibility to various antibiotics. Therefore, it is correct to say that stool specimens in Cary Blair medium can be used for C&S.Rate this question:
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- 5.
It is common for the patient to first rinse their mouth with plain water to reduce the number of oral (mouth) bacteria present that can contaminate the sputum sample
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True
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False
Correct Answer
A. TrueExplanation
Rinsing the mouth with plain water before collecting a sputum sample is a common practice to reduce the number of oral bacteria present in the mouth. This is important because these bacteria can contaminate the sputum sample, leading to inaccurate test results. By rinsing the mouth, the patient can minimize the presence of oral bacteria and ensure a more reliable sputum sample for testing. Therefore, the statement is true.Rate this question:
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- 6.
A sputum specimen may be canceled if there are too many oral bacteria present.
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True
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False
Correct Answer
A. TrueExplanation
A sputum specimen may be canceled if there are too many oral bacteria present. This is because oral bacteria can contaminate the sputum sample, making it difficult to accurately diagnose any respiratory infections or diseases. Therefore, if there are too many oral bacteria present in the specimen, it may be deemed unusable and the test may need to be canceled or repeated.Rate this question:
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- 7.
When transporting a liquid specimen like sputum for culture, the specimen should be placed in a biohazard transport bag in the exact same compartment of the bag as the paperwork.
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True
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False
Correct Answer
A. FalseExplanation
When transporting a liquid specimen like sputum for culture, the specimen should not be placed in the exact same compartment of the bag as the paperwork. This is because the specimen may leak or spill during transportation, potentially contaminating the paperwork and making it unreadable. Therefore, it is important to separate the specimen from the paperwork by placing it in a separate compartment or using a separate bag to ensure the safety and integrity of both the specimen and the paperwork.Rate this question:
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- 8.
Stool specimen in PVA and formalin can be used for C&S.
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True
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False
Correct Answer
A. FalseExplanation
Stool specimens in PVA (polyvinyl alcohol) and formalin are not suitable for culture and sensitivity (C&S) testing. PVA is used for preserving parasites and formalin is used for preserving tissues, but they are not appropriate for bacterial culture. Therefore, the statement is false.Rate this question:
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- 9.
Enterobius vermicularis can be collected by the use of
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A microscope slide, tongue depressor and cellophane tape system only
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Perianal pabble swab
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Both
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None
Correct Answer
A. BothExplanation
The correct answer is both. Enterobius vermicularis, also known as pinworm, can be collected using both the microscope slide, tongue depressor, and cellophane tape system, as well as the perianal swab method. The tape system is commonly used to collect pinworm eggs by pressing it against the perianal area to pick up any eggs present. The perianal swab method involves swabbing the area around the anus to collect any pinworm eggs or adult worms. Both methods are effective in collecting Enterobius vermicularis for examination and diagnosis.Rate this question:
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- 10.
Criteria for urine for C&S. Choose all that apply.
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Sterile cup
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Midstream clean catch collection
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Bleaching the urethra collection
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Aseptic collection technique
Correct Answer(s)
A. Sterile cup
A. Midstream clean catch collection
A. Aseptic collection techniqueExplanation
The criteria for urine for C&S testing include using a sterile cup to collect the urine sample, performing a midstream clean catch collection technique to minimize contamination, and using an aseptic collection technique to ensure a sterile sample. These criteria are important to prevent the introduction of bacteria or other contaminants into the urine sample, which could affect the accuracy of the culture and sensitivity testing.Rate this question:
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- 11.
Stool specimens can be collected from an infant's diaper.
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True
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False
Correct Answer
A. TrueExplanation
Stool specimens can indeed be collected from an infant's diaper. Since infants are not yet potty trained, using a diaper is the most practical way to collect a stool sample. A special collection pad or small bag can be used inside the diaper to catch the stool. After collection, the sample is transferred to a sterile container for analysis. It's important to ensure the sample is not contaminated during collection to ensure accurate results. This method is both convenient and effective for collecting stool specimens from infants.Rate this question:
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- 12.
What can be discovered in an O&P stool test?
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Protozoan cysts
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Protozoan trophozoites
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Helminthes ova (eggs)
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Larvae or immature forms of helminthes
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Chicken egg
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Arthropod mother
Correct Answer(s)
A. Protozoan cysts
A. Protozoan trophozoites
A. Helminthes ova (eggs)
A. Larvae or immature forms of helminthesExplanation
An O&P stool test is a laboratory test that is used to detect the presence of parasites in the digestive tract. The test can identify various forms of parasites, including protozoan cysts and trophozoites, helminthes ova (eggs), and larvae or immature forms of helminthes. These findings can help diagnose parasitic infections in individuals and guide appropriate treatment. The presence of a chicken egg and arthropod mother in the test would not be expected findings and may suggest contamination or other issues with the test.Rate this question:
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- 13.
Please choose all methods used to identify bacteria.
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Microscopic appearance
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Dancing technique on plate
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Colonial morphology
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Selective or indicating media
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Gram and other stains
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Biochemical reactions
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GENE PROBES
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ANTIBODY REACTIONS
Correct Answer(s)
A. Microscopic appearance
A. Colonial morphology
A. Selective or indicating media
A. Gram and other stains
A. Biochemical reactions
A. GENE PROBES
A. ANTIBODY REACTIONSExplanation
The methods used to identify bacteria include microscopic appearance, colonial morphology, selective or indicating media, gram and other stains, biochemical reactions, gene probes, and antibody reactions. These methods allow scientists to observe the physical characteristics of the bacteria, such as their size and shape under a microscope, as well as their appearance on agar plates. Selective or indicating media helps to isolate specific types of bacteria, while gram and other stains provide information about the cell wall composition. Biochemical reactions can be used to determine the metabolic capabilities of the bacteria. Gene probes and antibody reactions involve the use of specific genetic markers or antibodies to identify the presence of certain bacteria.Rate this question:
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- 14.
Choose all preservatives that can be used to preserve stool for parasite examination.
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10% formalin
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PVA (polyvinyl alcohol)
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Cary Blair
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C&S
Correct Answer(s)
A. 10% formalin
A. PVA (polyvinyl alcohol)Explanation
10% formalin and PVA (polyvinyl alcohol) are both commonly used preservatives for preserving stool samples for parasite examination. 10% formalin is a solution of formaldehyde gas dissolved in water, which helps to fix and preserve the stool sample by preventing decomposition and killing any parasites present. PVA is a synthetic polymer that forms a stable gel when mixed with water, which helps to preserve the stool sample by preventing dehydration and maintaining the structure of any parasites present. Cary Blair and C&S are not commonly used preservatives for stool samples in the context of parasite examination.Rate this question:
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- 15.
Choose all pathogenic organisms that are usually detected in a stool specimen.
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Salmonella
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Shigella
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Escherichia coli O157:H7
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Clostridium difficile
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Campylobacter
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Plasmodium
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HCG
Correct Answer(s)
A. Salmonella
A. Shigella
A. Escherichia coli O157:H7
A. Clostridium difficile
A. Campylobacter
A. PlasmodiumExplanation
The correct answer includes Salmonella, Shigella, Escherichia coli O157:H7, Clostridium difficile, Campylobacter, and Plasmodium. These organisms are commonly detected in stool specimens and are known to cause various gastrointestinal infections. Salmonella and Shigella are bacteria that can cause severe diarrhea and abdominal pain. Escherichia coli O157:H7 is a strain of E. coli that produces toxins and can cause bloody diarrhea. Clostridium difficile is a bacteria that can cause antibiotic-associated diarrhea. Campylobacter is a common cause of bacterial gastroenteritis. Plasmodium is a parasite that causes malaria. HCG is not a pathogenic organism and is not usually detected in stool specimens.Rate this question:
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Current Version
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Mar 18, 2025Quiz Edited by
ProProfs Editorial Team
Expert Reviewed by
Lindsey Block -
Jan 25, 2013Quiz Created by
Lola Agbetunsin