Quiz: Hardest Exam On Microbiology! Trivia

33 Questions

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Quiz: Hardest Exam On Microbiology! Trivia

Are you looking for a way to test how much you understand when it comes to microbiology? Below is what is considered the hardest exams created when it comes all the ones prepared on microbiology and not all people even attain half the marks. Give it a shot and see if you can attain the genius status of passing half of it.


Questions and Answers
  • 1. 
    Natually occurring enzymes that cut DNA into fragments, cut in predictable and controllable manner
    • A. 

      Primers

    • B. 

      Restriction Fragment

    • C. 

      Restriction enzymes

  • 2. 
    Restriction enzymes generates pieces of DNA called restriction fragments what do they do?
    • A. 

      These fragments can be joined to new fragments

    • B. 

      Enzymes produce jagged cuts called sticky ends or blunt cut called blunt ends/ ends anneal together to form new strand

    • C. 

      Naturally occuring enzymes that cut DNa into fragments

    • D. 

      Only B and C are correct

    • E. 

      Only A and B are correct

  • 3. 
    DNA ligase covalently joins fragments
    • A. 

      False

    • B. 

      True

  • 4. 
    Gel Electrophoresis...
    • A. 

      A highly purified form of agar, or polyacrylamide

    • B. 

      Used to separate DNA fragments according to size

    • C. 

      Forms a covalent bond between the sugar-phosphate residues of adjacent nucleotides

  • 5. 
    Put in correct order process of Gel electrophoresis1. DNA move through the gel towards positive electrode2. DNA is put into wells in gel 3.Gel subjected to current (negative to positive)
    • A. 

      1,3,2

    • B. 

      1,2,3

    • C. 

      2,3,1

    • D. 

      3,2,1

    • E. 

      2,1,3

  • 6. 
    • A. 

      Small fragment remain high in gel/ large fragments migrate lower

    • B. 

      Large fragments remain high in gel/ small fragments migrate lower

    • C. 

      Both large and small fragments remain high in gel

    • D. 

      Both large and small fragmetns migrate lower

  • 7. 
    Gel must be stained to veiw DNA
    • A. 

      True

    • B. 

      False

  • 8. 
    • A. 

      Used to locate nucleotide sequence in DNA or RNA strand

    • B. 

      Used to detect specific DNA sequences in colonies grown in agar plates

    • C. 

      Is single-stranded pice of DNA tagged with detectable marker (location can be easily determined)

    • D. 

      Uses to detect DNA sequences in restriction fragments seaparted using gel electrophoresis

    • E. 

      Will hybridize to complementary fragment of interest

  • 9. 
    • A. 

      Used to detect specific DNA sequences in colonies grown in agar plates (colonies are transferred in place on nylon membrane)

    • B. 

      Are used to determine which cells contain gene of interest

    • C. 

      Application is locating DNA sequences similar to ones being studied

    • D. 

      Used to located nucleotide sequence in DNA or RNA

    • E. 

      Will hybridze to complementary fragment of interest

  • 10. 
    • A. 

      Used to detect specific DNA sequences in colonies grown in agar plates

    • B. 

      Uses fluorescently labeled probe to detect certain nucleotide sequences

    • C. 

      Uses probes to detect DNA sequences in restriciton fragments separted using gel electrophoresis

    • D. 

      Application of Southern blotting is locating DNA sequences similar to ones being studed

    • E. 

      Can be used to identify specific properites of bacteria

  • 11. 
    Norther Blot is to Protein as Western Blot is to RNA
    • A. 

      True

    • B. 

      False

  • 12. 
    • A. 

      Uses fluorescently labeled probe to detect certain nucleotide sequences

    • B. 

      Uses fluorescently labeled probe to detect certain nucleotide sequences / detects sequences inside intact cell

    • C. 

      FISH can be used to identify specific properties of bacteria

    • D. 

      Used to detect specific DNA sequnces in colonies grown in agar plates

    • E. 

      Colony blots are used to determine which cells contain gene of interest

  • 13. 
    • A. 

      Enable researchers to screen sample for numerous sequences simultaneously

    • B. 

      DNA sequence analysis assists in studying evolutionary

    • C. 

      Used to study gene expression under certain conditon

    • D. 

      DNA sequence analysis assists in studying evolutionary relatedness

    • E. 

      Order of color reflects nucleotide sequence

  • 14. 
    • A. 

      I can hear your heart crying out for meeeeeeee

    • B. 

      DNA array are solid support

    • C. 

      Won't you listen to you're heart

    • D. 

      Entire DNA speciment to be studied is labeled

    • E. 

      Come on in come on in come on in and save me :)

  • 15. 
    Knowing DNA sequence of particular cell helps identify genetic alterations, alterations that may result in desease are...
    • A. 

      Sickle cell anemia

    • B. 

      HIV

    • C. 

      Cystic fibrosis

    • D. 

      Hemophilia

    • E. 

      Both A and C are correct

  • 16. 
    Sickle cell anemia is not due to single base-pair change in gene
    • A. 

      True

    • B. 

      False

  • 17. 
    Cystic fibrosis is caused by three base-pair deletion
    • A. 

      True

    • B. 

      False

  • 18. 
    DNA sequence analysis assists in studying evolutionary relatedness
    • A. 

      False

    • B. 

      True

  • 19. 
    Elements for termination includesingle stranded DNA templage, primer that anneal to template, DNA polymerase, Each of the nucleotide bases (one of these bases is labeled with marker for detection), dideoxynucleotides (like deoxynucleotide counterparts but lack 3' OH incorporation causes chain termination). What type of termination is this?
    • A. 

      Dideoxynucleotides termination

    • B. 

      Dideoxychain termination

    • C. 

      Chain terminators

    • D. 

      All of the above are correct

    • E. 

      None of the above are correct

  • 20. 
    Polyacrylamide gel electrophoresis used to separate DNA fragments by size
    • A. 

      False

    • B. 

      True

  • 21. 
    • A. 

      Electrophoresis used to separate DNA fragmetns by size

    • B. 

      Uses fluorescent dyes to detect newly synthesized DNA

    • C. 

      Gel electrophoresis used to separate fragmetns into colored bands

    • D. 

      Laser used to detect color differences (order of color reflects nucleotide sequence)

    • E. 

      DNA sequence analysis assits in studying evolutinoary relatedness

  • 22. 
    Primers
    • A. 

      Single-stranded DNA fragments bind the sequence of DNA, used in in vitro DNA synthesis, serves as fargment for additon of DNA nucleotides (PCR)

    • B. 

      Creates millions of copies of given region of DNA in matter of hours

    • C. 

      DNA is amplified exponentially

    • D. 

      Starting with double-stranded DNA molecule, process involves number of amplification cycles

  • 23. 
    Creates millions of copies of given region of DNA in matter of hours (technique exploits specificity of primers, allows for selective replication of chosen regions, termed target DNA, large amounts of DNA can be produced from very small sample are...
    • A. 

      DNA cloning

    • B. 

      Polymerase Chain Reaction

    • C. 

      Primers

    • D. 

      Fundamental Tools of Biotechnology

    • E. 

      I just want to take it nice and slow

  • 24. 
    In Polymerase Chain reaction, starting with double-stranded DNA molecule, process involves number of amplification cycle
    • A. 

      True

    • B. 

      False

  • 25. 
    PCR requires three step amplification cycle place the steps in order. Step 1: primers anneal to complementary sequence of target DNA  and DNA syntheiss occurs with heat stable DNA polymeraseStep 2: duplication of target DNA Step 3: double-stranded DNA denatured by heat
    • A. 

      1,2,3

    • B. 

      3,1,2

    • C. 

      2,1,3

    • D. 

      3,2,1

  • 26. 
    DNA is amplified exponentially
    • A. 

      True

    • B. 

      False

  • 27. 
    Process of producing copies of DNA is...
    • A. 

      RNA cloning

    • B. 

      DNA cloning

    • C. 

      PCR

  • 28. 
    Cloned DNA generally combined with carrier molecule called cloning vector, insures replication of target DNA.
    • A. 

      False

    • B. 

      True

  • 29. 
    Gene expression, regulation and function can be studied by gene fusion
    • A. 

      False

    • B. 

      True

  • 30. 
    Reporter gene encodes observable trait, trait makes it possible to determine the conditons that affect gene activity
    • A. 

      True

    • B. 

      False

  • 31. 
    Yeast is not an important eukaryotic model for gene function and regulation
    • A. 

      True

    • B. 

      False

  • 32. 
    Define transgenic organsim
    • A. 

      Plants or animals that receive engineered gene

    • B. 

      Joining gene being studed to reporter gene

    • C. 

      Researching gene function and regulation

    • D. 

      All of the above are correct

    • E. 

      None of the above are correct

  • 33. 
    Examples of genetically altered plants include... please check all that apply
    • A. 

      Pest-resistant plants (corn, cotton and potatoes)

    • B. 

      Herbicide- resistant plants (soybeans, cotton and corn)

    • C. 

      Plants with imporved nutrient value (rice)

    • D. 

      Plants as edible vaccines

    • E. 

      Plants are used for smoking weed