Biochemistry - Glycolysis & Gluconeogensis Questions

Reviewed by Editorial Team
The ProProfs editorial team is comprised of experienced subject matter experts. They've collectively created over 10,000 quizzes and lessons, serving over 100 million users. Our team includes in-house content moderators and subject matter experts, as well as a global network of rigorously trained contributors. All adhere to our comprehensive editorial guidelines, ensuring the delivery of high-quality content.
Learn about Our Editorial Process
| By Catherine Halcomb
Catherine Halcomb
Community Contributor
Quizzes Created: 1443 | Total Attempts: 6,686,485
| Attempts: 1,124
SettingsSettings
Please wait...
  • 1/13 Questions

    In glycolysis, the two priming reactions (requiring ATP) are? 

    • 1 & 3
    • 7 & 10
    • 6 & 4
    • 7 & 9
    • 3 & 7
Please wait...
About This Quiz

This quiz focuses on Glycolysis and Gluconeogenesis, assessing knowledge on reaction steps, energy yield, and enzyme activities. It is designed for learners to validate their understanding of these crucial metabolic pathways, enhancing their grasp of biochemistry fundamentals.

Metabolic Pathways Quizzes & Trivia

Quiz Preview

  • 2. 

    Which statement about glycolysis is correct

    • The reaction pathway liberates 1400 kJ/mol of energy

    • The pathway involves 10 reactions

    • 5 of the reactions in glycolysis are essentially irreversible

    • The coenzyme NADPH is required in the pathway

    • Involves the splitting of a 6 carbon compound into three 2 carbon compounds

    Correct Answer
    A. The pathway involves 10 reactions
    Explanation
    Glycolysis is a metabolic pathway that involves the breakdown of glucose into pyruvate. It is a series of 10 reactions that occur in the cytoplasm of cells. Each reaction is catalyzed by a specific enzyme, and the overall process results in the production of ATP and NADH. Therefore, the statement that "The pathway involves 10 reactions" is correct.

    Rate this question:

  • 3. 

    In glycolysis, most reactions have G values around zero except: 

    • Reactions 1, 3, 10

    • Reactions 2, 4, 5

    • Reactions 7 and 8

    • Reactions 9 and 10

    • Reaction 8 and 10

    Correct Answer
    A. Reactions 1, 3, 10
  • 4. 

    The irreversible reactions of glycolysis are bypassed in gluconeogenesis using the following enzymes:

    • Oxaloacetase, PFK-1, glycogen phosphorylase, debranching enzyme

    • Phosphoglucomutase, branching enzyme, glycogen synthase

    • PFK-1, Hexokinase, Aldolase

    • Glucose 6-phosphatase; fructose 1,6 bisphosphatase; Pyruvate carboxylase; PEP carboxykinase

    • NADH, FADH2, glucose, glycogen

    Correct Answer
    A. Glucose 6-phosphatase; fructose 1,6 bisphosphatase; Pyruvate carboxylase; PEP carboxykinase
    Explanation
    Gluconeogenesis is the process by which glucose is synthesized from non-carbohydrate precursors. It is essentially the reverse of glycolysis, with a few key differences. The irreversible reactions of glycolysis are bypassed in gluconeogenesis by using specific enzymes. Glucose 6-phosphatase is responsible for converting glucose 6-phosphate to glucose, allowing glucose to be released into the bloodstream. Fructose 1,6 bisphosphatase catalyzes the hydrolysis of fructose 1,6 bisphosphate to fructose 6-phosphate, an important step in reversing the committed step of glycolysis. Pyruvate carboxylase and PEP carboxykinase are involved in converting pyruvate to phosphoenolpyruvate (PEP), another key step in gluconeogenesis. These enzymes are necessary for the synthesis of glucose from non-carbohydrate sources.

    Rate this question:

  • 5. 

    In comparison with the resting state, actively contracting human muscle tissue has a

    • Higher concentrations of ATP

    • Higher rate of lactate formation

    • Lower consumption of glucose

    • Lower rate of consumption of oxygen

    • Lower ratio of NADH to NAD+

    Correct Answer
    A. Higher rate of lactate formation
    Explanation
    During muscle contraction, the demand for energy increases, leading to higher concentrations of ATP. To meet this demand, the breakdown of glucose is accelerated, resulting in a higher rate of lactate formation. This occurs when the breakdown of glucose proceeds faster than the rate at which it can be completely oxidized to produce ATP. Therefore, the higher rate of lactate formation is a result of the increased energy requirements during muscle contraction.

    Rate this question:

  • 6. 

    Which enzyme does NOT catalyse an irreversible reaction in either glycolysis or gluconeogenesis?

    • Hexokinase

    • Glucose 6-phosphatase

    • Pyruvate kinase

    • Phosphofructokinase-1

    • Enolase

    Correct Answer
    A. Enolase
    Explanation
    Enolase is the correct answer because it catalyzes a reversible reaction in both glycolysis and gluconeogenesis. It converts 2-phosphoglycerate to phosphoenolpyruvate in glycolysis and the reverse reaction in gluconeogenesis. The other enzymes listed in the options catalyze irreversible reactions in either glycolysis or gluconeogenesis. Hexokinase phosphorylates glucose to glucose 6-phosphate, glucose 6-phosphatase dephosphorylates glucose 6-phosphate to glucose, pyruvate kinase converts phosphoenolpyruvate to pyruvate, and phosphofructokinase-1 phosphorylates fructose 6-phosphate to fructose 1,6-bisphosphate.

    Rate this question:

  • 7. 

    Which statement about the energy production and usage in glycolysis and gluconeogenesis is correct?  

    • Glycolysis produces 2 ATP; gluconeogenesis requires 2 ATP

    • Glycolysis produces 32 ATP; gluconeogenesis produces 2 ATP

    • Glycolysis requires a net input of 2 ATP to run the final reaction (step 10); gluconeogenesis requires the net input of 6 ATP

    • Glycolysis uses 2 ATP but produces 4 ATP. Gluconeogenesis requires 4 ATP and 2 GTP

    • All enzymes in glycolysis and gluconeogenesis require energy in the form of ATP

    Correct Answer
    A. Glycolysis uses 2 ATP but produces 4 ATP. Gluconeogenesis requires 4 ATP and 2 GTP
    Explanation
    Glycolysis is a metabolic pathway that breaks down glucose to produce energy in the form of ATP. It uses 2 ATP molecules as an initial investment but ultimately produces 4 ATP molecules. This is due to the production of 4 ATP molecules through substrate-level phosphorylation. On the other hand, gluconeogenesis is a process that synthesizes glucose from non-carbohydrate sources. It requires 4 ATP and 2 GTP molecules for the conversion of pyruvate to glucose. Therefore, the statement "Glycolysis uses 2 ATP but produces 4 ATP. Gluconeogenesis requires 4 ATP and 2 GTP" is correct as it accurately describes the energy production and usage in both pathways.

    Rate this question:

  • 8. 

    The catabolism of 4 mol of glucose into 8 mol of pyruvate via glycolysis yields: 

    • 1 mol of ATP and 4 mol of NADH

    • 2 mol of ATP and 1 mol of NADH

    • 8 mol of ATP and 8 mol of NADH

    • 32 mol of ATP and 2 mol of NADH

    • None of the above

    Correct Answer
    A. 8 mol of ATP and 8 mol of NADH
    Explanation
    During the catabolism of 4 mol of glucose into 8 mol of pyruvate via glycolysis, a net of 8 mol of ATP is produced through substrate-level phosphorylation. Additionally, 8 mol of NADH is generated through the reduction of NAD+ to NADH. This occurs through the oxidation of glucose and subsequent formation of pyruvate. Therefore, the correct answer is 8 mol of ATP and 8 mol of NADH.

    Rate this question:

  • 9. 

    Following glycolysis, which of these molecules is NOT a possible fate of pyruvate catabolism?

    • Glycogen

    • CO2

    • Ethanol

    • Lactate

    • Acetyl CoA

    Correct Answer
    A. Glycogen
    Explanation
    Glycogen is not a possible fate of pyruvate catabolism because pyruvate cannot be directly converted into glycogen. Instead, pyruvate can be converted into CO2, ethanol, lactate, or Acetyl CoA through various metabolic pathways. Glycogen is a storage form of glucose and is synthesized from glucose molecules, not from pyruvate.

    Rate this question:

  • 10. 

    In glycolysis, the reaction(s) that produce(s) NADH is? 

    • Reaction 5

    • Reaction 6

    • Reactions 5 & 6

    • Reaction 9

    • Reaction 7

    Correct Answer
    A. Reaction 6
    Explanation
    In glycolysis, reaction 6 produces NADH. This reaction involves the conversion of glyceraldehyde-3-phosphate to 1,3-bisphosphoglycerate, with the reduction of NAD+ to NADH. This reaction is important because it generates NADH, which can then be used in the later steps of glycolysis and in other metabolic pathways.

    Rate this question:

  • 11. 

    State the stoichiometry of the glycolytic pathway, in relation to both ATP and NADH production. Describe the appropriate reactions in your answer. 

  • 12. 

    Describe the fates of pyruvate in both aerobic and anaerobic tissues. 

  • 13. 

    Explain the steps in the gluconeogenesis pathway which are not the reverse of reactions of the glycolysis pathway. 

Quiz Review Timeline (Updated): Mar 21, 2023 +

Our quizzes are rigorously reviewed, monitored and continuously updated by our expert board to maintain accuracy, relevance, and timeliness.

  • Current Version
  • Mar 21, 2023
    Quiz Edited by
    ProProfs Editorial Team
  • Apr 22, 2016
    Quiz Created by
    Catherine Halcomb
Back to Top Back to top
Advertisement
×

Wait!
Here's an interesting quiz for you.

We have other quizzes matching your interest.