Bacterial Transformation Quiz! Trivia Questions

10 Questions | Total Attempts: 1151

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Bacterial Transformation Quiz! Trivia Questions

What do you know about bacterial transformation? Do you assume you can pass this quiz? Bacterial transformation is a horizontal gene transfer process by which some bacteria take up foreign genetic material (naked DNA) from the environment. The four bacterial transformation steps are competent cell preparation, the transformation of cells, cell recovery, and cell plating. Taking this quiz will put your knowledge of bacterial transformation to the test.


Questions and Answers
  • 1. 
    DNA transformation involves the transfer of naked DNA into a recipient cell.
    • A. 

      True

    • B. 

      False

  • 2. 
    In the first step double-stranded donor DNA binds to specific receptors on the surface of a competent cell.
    • A. 

      True

    • B. 

      False

  • 3. 
    The purpose of surface proteins on bacterial species is to recognize and transport DNA from closely related bacterial species into the cell.
    • A. 

      True

    • B. 

      False

  • 4. 
    In Bacterial transformation, the bacteria take up and express foreign DNA usually in the form of a________
    • A. 

      Plasmid

    • B. 

      Enzyme

    • C. 

      Cofactor

    • D. 

      RNA

  • 5. 
    Does amplification occur after transformation?
    • A. 

      True

    • B. 

      Flase

  • 6. 
    In order to persist and be stably maintained in the cell, a plasmid DNA molecule must contain what?
    • A. 

      DNA

    • B. 

      RNA

    • C. 

      Proteins

    • D. 

      Origin of replication

  • 7. 
    Markers are used for identifying E.coli cells.
    • A. 

      True

    • B. 

      False

  • 8. 
    At what temperature should E. coli be incubated?
    • A. 

      10 C

    • B. 

      15 C

    • C. 

      20 C

    • D. 

      37 C

  • 9. 
    The purpose of calcium chloride is to allow the plasmid to pass through the bacterial cell wall.
    • A. 

      True

    • B. 

      False

  • 10. 
    Electroporation is a mechanical method used to introduce polar molecules into a host cell through the cell membrane.
    • A. 

      True

    • B. 

      False