Lab 2

The correct answer refers to a Milk Plate testing for Caseinase activity resulting in clearing of the plate.

The Citrate test is used to determine if an organism can utilize Citrate as a sole carbon source. Negative result implies the organism cannot use Citrate. Simmons Citrate Slant is the specialized media used for this test.

The correct answer describes the results of the pigment test with M. roseus on NA slant. The incorrect answers provide different scenarios with other organisms and media that do not match the given question.

The correct answer describes the Carbohydrate fermentation test, which tests for fermentation of sugars and results in acid production. The incorrect answers provide alternative tests with different purposes and results to confuse the test taker.

The Lys Test specifically tests for Lysine Decarboxylase enzyme. A negative result indicates the absence of this enzyme in the test tube.

In the citrate test, a positive result indicates that the bacterium is able to utilize citrate as its sole carbon source, leading to alkaline byproducts and a change in pH of the medium.

Barritt's reagent A is specifically used in the Voges-Proskauer test to detect the presence of acetoin, which results in a red color in the test tube. Therefore, a red color in this test indicates a positive result for non acidic fermentation.

The question is asking for the tests associated with specific reagents and their respective uses. The correct answer provides the tests MR test, Starch testMixed acid ferm. Caseinase. The incorrect answers were chosen to provide a variety of different tests and differentiate them from the correct answer. This multiple-choice question aims to test knowledge of laboratory tests and reagents.

The correct answer indicates a specific set of results observed in the tubes on the left and right side. The incorrect answers either suggest variations in pH or presence of fermentation, which are not consistent with the original scenario described in the question.

The correct answer is Catalase, as it is a common test used to detect the presence of the enzyme catalase in biological samples.

The correct answer indicates a positive result for carbohydrate fermentation with acid and gas production, which are commonly used indicators for certain tests. The incorrect answers provide different scenarios that do not align with the expected outcome of the test.

The correct answer discusses the H2S test using Peptone iron deep media and the positive result for cystein desulfurase. The incorrect answers provide different tests, media, and results, showcasing the importance of understanding specific test procedures.

In the provided question and correct answer, line A represents the killing dose of streptomycin and line B represents highly resistant colonies based on the experiment being referenced.

The correct result for this test is positive for lysine decarboxylase, which is indicated by the + sign. Arginine decarboxylase, ornithine decarboxylase, and threonine decarboxylase are not the correct results and therefore considered incorrect answers.

The Phe test is specifically used to test for the presence of phe aminase, and in a positive reaction, it produces a green color.

The urease test is conducted using urea slant as the media to detect the presence of urease enzyme and the ability of bacteria to hydrolyze urea. A positive result for this test indicates the bacteria's ability to produce urease.

IMViC test is a set of four biochemical tests used for the differentiation of Enterobacteriaceae based on their ability to ferment glucose (Glucose Fermentation), produce methyl red (MR), produce acetoin (Voges-Proskauer, VP), and utilize citrate as the sole carbon source. Indole test is used to determine the presence of tryptophanase enzyme in bacteria, where Kovac's reagent is used to detect the formation of indole.

The correct plate is EMB Plate which tests for G- or G+ organisms. The growth characteristics and color changes on the plate determine the result of the test.

A PEA (Phenylethyl Alcohol) plate is selective for Gram-positive bacteria due to the phenylethyl alcohol inhibiting the growth of Gram-negative bacteria.

The Gelatin test is to determine the presence of gelatinase enzymes. A positive result indicates the ability of the microorganism to hydrolyze gelatin.

Testing motility involves assessing the ability of microorganisms to move. Soft agar stab inoculation and hanging drop technique are common methods used to determine motility in bacteria. Blood agar plate streaking, agglutination testing, and acid-fast staining are not specific methods for testing motility.

Enterobacteriaceae are typically Bacillus-shaped, oxidase negative bacteria that produce acid in glucose fermentation. Other characteristics mentioned in the incorrect answers do not match the typical characteristics of this bacterial family.