Quiz On The Terms Of Biotechniques

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| By Eric James

Eric James

Community Contributor

Quizzes Created: 1 | Total Attempts: 446

Questions: 8 | Attempts: 446 | Updated: Mar 19, 2023

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The term “biotechnique” is given to any form of technique used in the study of biotechnology – i. E. The exploitation of biological processes for industrial and other processes. What do you know about these techniques? Let’s find out!

Questions and Answers

1.

1. A handy tool for determining the differences between two cell types.
- A.
  
  DNA Extraction
- B.
  
  DNA Microarray
- C.
  
  Gel Electrophoresis
- D.
  
  Polymerase Chain Reaction
Correct Answer
B. DNA Microarray

Explanation
A DNA microarray is a handy tool for determining the differences between two cell types. It allows researchers to compare the expression levels of thousands of genes simultaneously. By using DNA probes that are specific to different genes, the microarray can detect and measure the amount of mRNA present in each cell type. This information can then be used to identify genes that are differentially expressed between the two cell types and gain insights into their functional differences.

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1
0
2.

1. A type of RNA that always end in a sequence of adenines.
- A.
  
  Messenger RNA (mRNA)
- B.
  
  Transfer RNA (tRNA)
- C.
  
  Ribosomal RNA (rRNA)
- D.
  
  None of the above
Correct Answer
A. Messenger RNA (mRNA)

Explanation
Messenger RNA (mRNA) is a type of RNA that carries genetic information from DNA to the ribosomes in the cytoplasm. It is synthesized in the nucleus and contains a poly-A tail at its 3' end, which is a sequence of adenines. This poly-A tail helps to stabilize the mRNA molecule and regulate its stability and translation efficiency. Therefore, mRNA is the correct answer as it is the only type of RNA that always ends in a sequence of adenines.

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1
0
3.

1. Substance used to wash RNA which will only bind to RNA strands that have a poly-A tail.
- A.
  
  Buffer Solution
- B.
  
  Columns with Oligo-dT beads
- C.
  
  Solvent Solution
- D.
  
  Microarray Washing Solution
Correct Answer
B. Columns with Oligo-dT beads

Explanation
Columns with Oligo-dT beads are used to wash RNA because they have a poly-A tail. The poly-A tail is a sequence of adenine nucleotides that is added to the 3' end of mRNA during post-transcriptional processing. The Oligo-dT beads have a sequence of thymine nucleotides that can bind specifically to the poly-A tail of mRNA. By washing the RNA sample with these beads, only the RNA strands that have a poly-A tail will bind to the beads, allowing for the isolation and purification of mRNA from the sample.

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4.

1. Why do we have to make a complementary DNA? Why not just use mRNA?
- A.
  
  Because our sample is DNA.
- B.
  
  It’s because that’s the result and we can’t do anything about it.
- C.
  
  DNA is much more stable than RNA.
- D.
  
  None of the above.
Correct Answer
C. DNA is much more stable than RNA.

Explanation
Complementary DNA (cDNA) is made from mRNA using the enzyme reverse transcriptase. This process allows for the conversion of RNA into DNA, which is more stable and easier to work with in the laboratory. DNA is less prone to degradation and can be stored for longer periods of time compared to RNA. Additionally, cDNA allows for the amplification and analysis of specific genes of interest. Therefore, making cDNA from mRNA is preferred over using mRNA directly in many molecular biology applications.

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5.

1. Which among the following is not a component of labeling mix?
- A.
  
  Poly-T primers
- B.
  
  Oligo-dT beads
- C.
  
  Reverse transcriptase
- D.
  
  Labeled nucleotides
Correct Answer
B. Oligo-dT beads

Explanation
Oligo-dT beads are not a component of labeling mix. The labeling mix typically consists of Poly-T primers, reverse transcriptase, and labeled nucleotides. Oligo-dT beads are used for isolating and purifying mRNA, but they are not directly involved in the labeling process.

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6.

1. A key property of DNA.
- A.
  
  Double stranded
- B.
  
  Sugar
- C.
  
  Phosphates
- D.
  
  Complementary base pairing
Correct Answer
D. Complementary base pairing

Explanation
Complementary base pairing is a key property of DNA. This refers to the specific pairing of nucleotide bases, where adenine (A) always pairs with thymine (T), and guanine (G) always pairs with cytosine (C). This pairing is essential for DNA replication and transcription, as it ensures that the genetic information is accurately transferred. The complementary base pairing also allows for the formation of the DNA double helix structure, where two strands of DNA are held together by hydrogen bonds between the paired bases.

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7.

1. A process where two complementary DNA strands from different sources can pair with one another.
- A.
  
  Translation
- B.
  
  Transcription
- C.
  
  Hybridization
- D.
  
  Synthesis
Correct Answer
C. Hybridization

Explanation
Hybridization refers to a process in which two complementary DNA strands from different sources can pair with one another. This can occur when single-stranded DNA or RNA molecules bind to their complementary sequences. Hybridization is commonly used in molecular biology techniques such as DNA sequencing, polymerase chain reaction (PCR), and DNA microarray analysis. It allows scientists to study and manipulate DNA by creating hybrid molecules that contain genetic information from different sources.

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8.

1. A material used to analyze the date from microarray.
- A.
  
  Microarray Database
- B.
  
  Microarray Scanner
- C.
  
  Microarray Microscope
- D.
  
  All of the above
Correct Answer
C. Microarray Microscope

Explanation
A microarray microscope is a tool used to analyze the data from a microarray. It is specifically designed to visualize and capture images of the microarray slide, allowing researchers to examine the patterns and intensity of gene expression. This microscope is equipped with high-resolution optics and advanced imaging software to accurately interpret the data and provide valuable insights into gene expression levels. It is an essential instrument in the field of genomics and plays a crucial role in understanding the complex biological processes.

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Current Version
Mar 19, 2023

Quiz Edited by
ProProfs Editorial Team
Sep 14, 2009

Quiz Created by
Eric James

Quiz On The Terms Of Biotechniques

1. A handy tool for determining the differences between two cell types.

1. A type of RNA that always end in a sequence of adenines.

1. Substance used to wash RNA which will only bind to RNA strands that have a poly-A tail.

1. Why do we have to make a complementary DNA? Why not just use mRNA?

1. Which among the following is not a component of labeling mix?

1. A key property of DNA.

1. A process where two complementary DNA strands from different sources can pair with one another.

1. A material used to analyze the date from microarray.